Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00287
         (504 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC830.08c |||Golgi membrane protein |Schizosaccharomyces pombe...    64   9e-12
SPBC30D10.09c |||HVA22/TB2/DP1 family protein|Schizosaccharomyce...    31   0.13 
SPAC20G8.04c |||mitochondrial electron transfer flavoprotein-ubi...    29   0.52 
SPCC61.05 |||S. pombe specific multicopy membrane protein family...    27   2.1  
SPAC15A10.06 |||CPA1 sodium ion/proton antiporter|Schizosaccharo...    26   2.8  
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    26   2.8  
SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces ...    25   6.5  
SPBC660.17c |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    25   6.5  

>SPCC830.08c |||Golgi membrane protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 182

 Score = 64.5 bits (150), Expect = 9e-12
 Identities = 26/72 (36%), Positives = 45/72 (62%)
 Frame = +2

Query: 38  EQKVGVNRVYIFLGLVAFTGLYLVFGFGAELICNSIGFVYPAYMSMKALESPQKDDDTKW 217
           E+  GV+++Y+FL       L+L   +G  L+ N + F  PA+ S+ A+E+  K DDT+W
Sbjct: 28  EKNFGVSKLYVFLTAAGIYALFLFLNWGGFLLTNLLAFAMPAFFSINAIETTNKADDTQW 87

Query: 218 LTYWVVYACFSI 253
           LTY++V +  ++
Sbjct: 88  LTYYLVTSFLNV 99



 Score = 61.3 bits (142), Expect = 8e-11
 Identities = 27/53 (50%), Positives = 37/53 (69%)
 Frame = +1

Query: 256 EYFSDFIVGWFPLYWLLKCIFVIWCYLPTEYNGSLVIYYRIIRPYYQKHHGRI 414
           EY+S  I+ + P+YWLLK IF+IW  LP ++NG+ +IY  +IRPY   H  RI
Sbjct: 101 EYWSQLILYYVPVYWLLKAIFLIWLALP-KFNGATIIYRHLIRPYITPHVIRI 152


>SPBC30D10.09c |||HVA22/TB2/DP1 family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 217

 Score = 30.7 bits (66), Expect = 0.13
 Identities = 14/48 (29%), Positives = 21/48 (43%)
 Frame = +1

Query: 277 VGWFPLYWLLKCIFVIWCYLPTEYNGSLVIYYRIIRPYYQKHHGRIDD 420
           + W P Y   K +F +W   P    G+  IY   I P+   H   I++
Sbjct: 124 LSWVPFYSTSKIVFWLWLLNP-RTQGAAFIYASYISPFLSDHKAAINN 170


>SPAC20G8.04c |||mitochondrial electron transfer
           flavoprotein-ubiquinone
           oxidoreductase|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 632

 Score = 28.7 bits (61), Expect = 0.52
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = -1

Query: 123 APKPNTKYKPVNATKPKKMYTLFTPTFCSASS 28
           A K  +KYKP+N  KP  + +   PT  S S+
Sbjct: 510 ATKSASKYKPINYPKPDNVLSFDIPTSVSRSA 541


>SPCC61.05 |||S. pombe specific multicopy membrane protein family
           1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 469

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 16/64 (25%), Positives = 29/64 (45%)
 Frame = +1

Query: 277 VGWFPLYWLLKCIFVIWCYLPTEYNGSLVIYYRIIRPYYQKHHGRIDDMANTVAWLVVTR 456
           +G+  L WLL+CI       P + + +  ++  +    YQ +        +   W VV+ 
Sbjct: 210 LGFITLTWLLRCIKSQSGVQPAQISLAFWVFIFVFTHSYQVYSMVAIGRGSFSTWYVVSF 269

Query: 457 LFNL 468
           LF+L
Sbjct: 270 LFSL 273


>SPAC15A10.06 |||CPA1 sodium ion/proton
           antiporter|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 569

 Score = 26.2 bits (55), Expect = 2.8
 Identities = 13/37 (35%), Positives = 22/37 (59%)
 Frame = +2

Query: 2   KSKPWTKYFELAEQKVGVNRVYIFLGLVAFTGLYLVF 112
           K+K  TKY      ++  N ++I+LG+  FT + LV+
Sbjct: 311 KAKLSTKYVFRVLAQLSENFIFIYLGMSLFTQVDLVY 347


>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
           Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 26.2 bits (55), Expect = 2.8
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = +3

Query: 213 NGLHIGWCTPVFLSGVLFRFH 275
           NGL   WC+ +FL G L  F+
Sbjct: 149 NGLLSDWCSNIFLPGYLLFFY 169


>SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 253

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 15/53 (28%), Positives = 25/53 (47%)
 Frame = -1

Query: 279 DDEIGKVLPIEKQAYTTQYVSHFVSSSFCGDSRAFIDMYAGYTKPIELHINSA 121
           +DE+ KV+P++KQ    Q  + F +    GDS   + +     K +   I  A
Sbjct: 76  NDEVMKVVPVQKQTRAGQR-TRFKAFVVIGDSDGHVGLGIKCAKEVATAIRGA 127


>SPBC660.17c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 172

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 15/46 (32%), Positives = 26/46 (56%)
 Frame = +1

Query: 208 YKMAYILGGVRLFFYREYFSDFIVGWFPLYWLLKCIFVIWCYLPTE 345
           +   Y+L  V LF Y EY++  +V    L++   C +V++ +LP E
Sbjct: 51  FSFLYLLLIVPLFLYPEYWAFKLVELLSLFY---CAYVMY-FLPLE 92


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,308,195
Number of Sequences: 5004
Number of extensions: 51029
Number of successful extensions: 144
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 200198394
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)