BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00274 (748 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 130 2e-31 SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 83 4e-17 SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 75 1e-14 SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schiz... 73 3e-14 SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S... 66 5e-12 SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo... 59 8e-10 SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 55 9e-09 SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 53 5e-08 SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pomb... 47 3e-06 SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 38 0.002 SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||... 37 0.003 SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po... 35 0.011 SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomy... 33 0.033 SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosac... 33 0.057 SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces p... 32 0.100 SPBC947.01 |||AAA family ATPase, unknown biological role|Schizos... 31 0.17 SPCC24B10.10c |||mitochondrial outer membrane ATPase Msp1 |Schiz... 31 0.17 SPBC32H8.09 |||WD repeat protein, human WDR8 family|Schizosaccha... 27 2.1 SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|c... 27 2.8 SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 27 2.8 SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 27 3.8 SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 26 5.0 SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosacchar... 26 6.6 SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharo... 26 6.6 SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 8.7 SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 25 8.7 SPCC16A11.06c |gpi10||pig-B|Schizosaccharomyces pombe|chr 3|||Ma... 25 8.7 >SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizosaccharomyces pombe|chr 3|||Manual Length = 389 Score = 130 bits (314), Expect = 2e-31 Identities = 58/78 (74%), Positives = 71/78 (91%) Frame = +2 Query: 497 IVEASASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIREAVE 676 +++ SASVAL +HSNALVD+LPPEADSSISML+ DE+PDV Y+D+GG+D QKQE+REAVE Sbjct: 92 LLKPSASVALQRHSNALVDILPPEADSSISMLRPDERPDVSYADVGGLDVQKQEVREAVE 151 Query: 677 LPLTHVELYRQIGIEPPR 730 LPLT +LYRQIGI+PPR Sbjct: 152 LPLTQGDLYRQIGIDPPR 169 Score = 122 bits (294), Expect = 5e-29 Identities = 57/80 (71%), Positives = 71/80 (88%) Frame = +3 Query: 258 KYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFLEAVDQNTGI 437 ++K L+R LE L++QE +IK++ ++LK+E + AQEEVKRIQSVPLVIGQFLEA+DQNT I Sbjct: 12 RWKALERQLEMLDLQEGFIKEDCKSLKRELIRAQEEVKRIQSVPLVIGQFLEAIDQNTAI 71 Query: 438 VGSTTGSNYYVRILSTIDRE 497 VGSTTGSNY VRILST+DRE Sbjct: 72 VGSTTGSNYVVRILSTLDRE 91 >SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 83.0 bits (196), Expect = 4e-17 Identities = 36/78 (46%), Positives = 57/78 (73%) Frame = +2 Query: 497 IVEASASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIREAVE 676 ++E SV LH + ++V +L + D I++++ D+ P Y+DIGG+++Q QEI+EAVE Sbjct: 149 MLEPGCSVLLHHKAMSIVGLLLDDTDPMINVMKLDKAPTESYADIGGLESQIQEIKEAVE 208 Query: 677 LPLTHVELYRQIGIEPPR 730 LPLTH ELY ++GI+PP+ Sbjct: 209 LPLTHPELYEEMGIKPPK 226 Score = 54.4 bits (125), Expect = 2e-08 Identities = 30/80 (37%), Positives = 50/80 (62%), Gaps = 3/80 (3%) Frame = +3 Query: 267 KLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEE---VKRIQSVPLVIGQFLEAVDQNTGI 437 K+QR+ + L ++EEY+++++R LK + QEE V I+ P+ +G E +D + I Sbjct: 71 KMQRIHDHLLMEEEYVQNQER-LKPQDERTQEERNRVDEIRGTPMSVGTLEEIIDDDHAI 129 Query: 438 VGSTTGSNYYVRILSTIDRE 497 V ST G YYV I+S +D++ Sbjct: 130 V-STAGPEYYVSIMSFVDKD 148 >SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit Rpt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 74.5 bits (175), Expect = 1e-14 Identities = 29/73 (39%), Positives = 50/73 (68%) Frame = +2 Query: 518 VALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIREAVELPLTHVE 697 + ++K S ++D LP E DS + ++ DEKP +YSDIGG+ Q +E+ EA+ LP+ + Sbjct: 150 IGVNKDSYLIIDKLPSEYDSRVKAMEVDEKPTERYSDIGGLSKQIEELFEAIVLPMQQAD 209 Query: 698 LYRQIGIEPPRVC 736 +R++G++PP+ C Sbjct: 210 KFRKLGVKPPKGC 222 >SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 73.3 bits (172), Expect = 3e-14 Identities = 32/77 (41%), Positives = 49/77 (63%) Frame = +2 Query: 500 VEASASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIREAVEL 679 +E V ++ A+ LPP+ D S++M+Q +EKPDV Y D+GG Q + +RE VEL Sbjct: 138 IEEGMRVGCDRNKYAIQLPLPPKIDPSVTMMQVEEKPDVTYGDVGGCKEQIERLREVVEL 197 Query: 680 PLTHVELYRQIGIEPPR 730 PL E + ++GI+PP+ Sbjct: 198 PLLSPERFVKLGIDPPK 214 >SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|Schizosaccharomyces pombe|chr 2|||Manual Length = 403 Score = 66.1 bits (154), Expect = 5e-12 Identities = 27/77 (35%), Positives = 48/77 (62%) Frame = +2 Query: 500 VEASASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIREAVEL 679 ++ + VAL S L+ +LP + D +S++ ++ PD Y +GG++ Q +EI+E +EL Sbjct: 104 IKPNIRVALRNDSYQLIKILPNKVDPLVSLMMVEKIPDSTYEMVGGLEKQIKEIKEVIEL 163 Query: 680 PLTHVELYRQIGIEPPR 730 P+ H EL+ +GI P+ Sbjct: 164 PVKHPELFESLGIPQPK 180 >SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizosaccharomyces pombe|chr 3|||Manual Length = 388 Score = 58.8 bits (136), Expect = 8e-10 Identities = 23/71 (32%), Positives = 47/71 (66%) Frame = +2 Query: 518 VALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIREAVELPLTHVE 697 V+L + ++ +LP E D + + ++ D+ ++ +GG++ Q +E+RE +ELPL + E Sbjct: 97 VSLDMTTLTIMRILPREVDPLVYNMSIEDPGDISFAGVGGLNEQIRELREVIELPLKNPE 156 Query: 698 LYRQIGIEPPR 730 L+ ++GI+PP+ Sbjct: 157 LFLRVGIKPPK 167 Score = 40.7 bits (91), Expect = 2e-04 Identities = 18/56 (32%), Positives = 35/56 (62%) Frame = +3 Query: 327 RNLKKEYLHAQEEVKRIQSVPLVIGQFLEAVDQNTGIVGSTTGSNYYVRILSTIDR 494 R+L K+Y ++++K +QSV +IG+ L+ +D IV +++G Y V + +D+ Sbjct: 33 RDLVKKYDKTEDDIKSLQSVGQIIGEVLKQLDSERFIVKASSGPRYVVGCRNNVDQ 88 >SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 55.2 bits (127), Expect = 9e-09 Identities = 21/43 (48%), Positives = 33/43 (76%) Frame = +2 Query: 602 EKPDVQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPPR 730 E P+V +SDIGG + KQ+++E+VE PLTH E + ++G+ PP+ Sbjct: 541 ESPNVHWSDIGGQEEVKQKLKESVEWPLTHGETFSRLGVRPPK 583 Score = 40.7 bits (91), Expect = 2e-04 Identities = 18/39 (46%), Positives = 25/39 (64%) Frame = +2 Query: 614 VQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPPR 730 V +S IGG+ Q +IR+ VELP + EL++ I PPR Sbjct: 276 VTFSSIGGLQAQIAQIRDIVELPFQNPELFKFFNIMPPR 314 >SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 52.8 bits (121), Expect = 5e-08 Identities = 23/40 (57%), Positives = 29/40 (72%) Frame = +2 Query: 611 DVQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPPR 730 +V Y DIGG Q +IRE VELPL H +L++ IGI+PPR Sbjct: 220 EVGYDDIGGCRRQMAQIRELVELPLRHPQLFKSIGIKPPR 259 Score = 45.2 bits (102), Expect = 1e-05 Identities = 16/43 (37%), Positives = 31/43 (72%) Frame = +2 Query: 602 EKPDVQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPPR 730 E P+V++ DIGG++ K+E+RE V++P+ + E + + G+ P + Sbjct: 490 EVPNVRWEDIGGLEEVKRELRETVQMPVMYAEKFLRFGVTPSK 532 >SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 779 Score = 46.8 bits (106), Expect = 3e-06 Identities = 21/48 (43%), Positives = 31/48 (64%), Gaps = 2/48 (4%) Frame = +2 Query: 593 QADEKP--DVQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPPR 730 +A+ +P D+ SDIGG+D E+ E V +P+ H E+Y+ GI PPR Sbjct: 162 EANREPPSDISLSDIGGLDDCINELLELVAMPIKHPEVYQYTGIHPPR 209 Score = 33.9 bits (74), Expect = 0.025 Identities = 13/40 (32%), Positives = 25/40 (62%) Frame = +2 Query: 608 PDVQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPP 727 P V +++IG + + + E++ A+ P+ ELY+ +GI P Sbjct: 487 PGVSWNNIGALKSIRVELQMAIVQPIKRPELYQSVGISAP 526 >SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1190 Score = 37.5 bits (83), Expect = 0.002 Identities = 12/39 (30%), Positives = 26/39 (66%) Frame = +2 Query: 614 VQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPPR 730 + + +GG+D +++E V LPL + E++++ ++PPR Sbjct: 263 LSFESVGGLDNYINQLKEMVMLPLLYPEIFQRFNMQPPR 301 >SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 948 Score = 37.1 bits (82), Expect = 0.003 Identities = 16/39 (41%), Positives = 26/39 (66%) Frame = +2 Query: 608 PDVQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEP 724 P V + DIGG++ K +R+ ++LPL EL+ Q G++P Sbjct: 650 PKVNWDDIGGLEEAKTVLRDTLQLPLQFPELFSQ-GLKP 687 >SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 35.1 bits (77), Expect = 0.011 Identities = 16/56 (28%), Positives = 33/56 (58%) Frame = +2 Query: 536 SNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIREAVELPLTHVELY 703 +N +D + S+++ EKP+V++ DI G++ K+ ++E V LP+ +L+ Sbjct: 100 ANEALDSDAKKLRSALTSAILVEKPNVRWDDIAGLENAKEALKETVLLPIKLPQLF 155 >SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1201 Score = 33.5 bits (73), Expect = 0.033 Identities = 13/42 (30%), Positives = 27/42 (64%) Frame = +2 Query: 605 KPDVQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPPR 730 + ++ ++ IGG++ +++E V LPL + E++ + I PPR Sbjct: 366 RENLDFNSIGGLEDIILQLKEMVMLPLLYPEVFLHLHITPPR 407 >SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosaccharomyces pombe|chr 2|||Manual Length = 506 Score = 32.7 bits (71), Expect = 0.057 Identities = 14/52 (26%), Positives = 28/52 (53%), Gaps = 1/52 (1%) Frame = -3 Query: 449 GAANYACILINCLK-KLSNDKRNRLYALYFFLCMQIFFLQVSLFILNVLFLY 297 G Y C+++ + ++N +N+ A FF C+ + F Q++L+ +F Y Sbjct: 182 GHFQYNCVMLGLVMYAIANLLKNQYVAATFFFCLALTFKQMALYFAPPIFFY 233 >SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces pombe|chr 2|||Manual Length = 773 Score = 31.9 bits (69), Expect = 0.100 Identities = 15/52 (28%), Positives = 29/52 (55%) Frame = +2 Query: 575 SSISMLQADEKPDVQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPPR 730 S M + ++++D+ G+D K+EI E V+ L + + Y ++G + PR Sbjct: 280 SRAKMFNHETDIKIKFADVAGVDEAKEEIMEFVKF-LKNPKFYERLGAKIPR 330 >SPBC947.01 |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 660 Score = 31.1 bits (67), Expect = 0.17 Identities = 15/38 (39%), Positives = 22/38 (57%) Frame = +2 Query: 614 VQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPP 727 V +SDI G+D K ++EAV P EL++ G+ P Sbjct: 376 VYWSDIAGLDDAKNSLKEAVIYPFLRPELFQ--GLREP 411 >SPCC24B10.10c |||mitochondrial outer membrane ATPase Msp1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 355 Score = 31.1 bits (67), Expect = 0.17 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = +2 Query: 584 SMLQADEKPDVQYSDIGGMDTQKQEIREAVELPLTHVELY 703 S L + DV + DIGGMD ++ + V PL + E++ Sbjct: 76 SQLVLPSEIDVSFDDIGGMDEHVNQLLQDVLFPLKYPEVF 115 >SPBC32H8.09 |||WD repeat protein, human WDR8 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 483 Score = 27.5 bits (58), Expect = 2.1 Identities = 11/33 (33%), Positives = 15/33 (45%) Frame = -1 Query: 370 FTSSCACKYSFFKFRCSSLMYSSCTSKNSNILW 272 F C S +F C ++ TSK N+LW Sbjct: 313 FPEPSFCSASKIRFNCDETYAATITSKYPNVLW 345 >SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 581 Score = 27.1 bits (57), Expect = 2.8 Identities = 14/37 (37%), Positives = 21/37 (56%) Frame = +2 Query: 551 DVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEI 661 D L + D + +Q+D KPDV+ SD MD K ++ Sbjct: 255 DQLTVKEDFDLKDMQSDIKPDVEKSD--AMDDSKMKV 289 >SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 709 Score = 27.1 bits (57), Expect = 2.8 Identities = 13/40 (32%), Positives = 25/40 (62%) Frame = +2 Query: 611 DVQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPPR 730 +V++SD+ G+D K+E+ E V+ L + ++G + PR Sbjct: 263 NVRFSDVQGVDEAKEELEEIVDF-LRDPTHFTRLGGKLPR 301 >SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces pombe|chr 2|||Manual Length = 262 Score = 26.6 bits (56), Expect = 3.8 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = +3 Query: 252 ITKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLH 353 I+ Y+++QR+LE LE++E D L+ Y+H Sbjct: 92 ISDYQQIQRLLEKLEIKEGNYGDGYA-LQPSYIH 124 >SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 347 Score = 26.2 bits (55), Expect = 5.0 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +3 Query: 336 KKEYLHAQEEVKRIQSVPLVIGQFLEAVDQNTGI 437 K ++ A E+ + VPL +F + VDQN G+ Sbjct: 51 KSKHSEAVVEILKSAKVPLKNKEFSDLVDQNIGV 84 >SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 682 Score = 25.8 bits (54), Expect = 6.6 Identities = 11/27 (40%), Positives = 19/27 (70%) Frame = -3 Query: 380 LYALYFFLCMQIFFLQVSLFILNVLFL 300 +++L+FFLCM+ FF +++ LFL Sbjct: 360 IFSLFFFLCMRNFFPGFTIY-FPALFL 385 >SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharomyces pombe|chr 1|||Manual Length = 672 Score = 25.8 bits (54), Expect = 6.6 Identities = 10/19 (52%), Positives = 16/19 (84%) Frame = -1 Query: 295 SKNSNILWSFLYFVINLQI 239 SK+ NILW +L+ ++NL+I Sbjct: 413 SKHLNILWLWLFRMLNLRI 431 >SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 25.4 bits (53), Expect = 8.7 Identities = 10/21 (47%), Positives = 16/21 (76%) Frame = +3 Query: 366 VKRIQSVPLVIGQFLEAVDQN 428 +K+ +SVP + GQF+E V+ N Sbjct: 193 LKKSKSVPRLRGQFMEPVEHN 213 >SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 231 Score = 25.4 bits (53), Expect = 8.7 Identities = 8/23 (34%), Positives = 15/23 (65%) Frame = +3 Query: 51 SSTTLCLCVRHISDSQIIIR*YT 119 + T+ C RH+ SQ++I+ Y+ Sbjct: 93 NKTSAVFCKRHVGSSQVVIKIYS 115 >SPCC16A11.06c |gpi10||pig-B|Schizosaccharomyces pombe|chr 3|||Manual Length = 506 Score = 25.4 bits (53), Expect = 8.7 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = -3 Query: 380 LYALYFFLCMQIFFLQVSLFILNVL 306 LYAL + IF L VSLF++N++ Sbjct: 224 LYALGYGRLFGIFVLCVSLFLVNII 248 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,905,891 Number of Sequences: 5004 Number of extensions: 57358 Number of successful extensions: 198 Number of sequences better than 10.0: 27 Number of HSP's better than 10.0 without gapping: 181 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 198 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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