Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00270
(727 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g10180.1 68416.m01219 kinesin motor protein-related similar t... 35 0.048
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 34 0.083
At4g36520.1 68417.m05185 trichohyalin-related low similarity to ... 31 0.78
At3g47180.1 68416.m05123 zinc finger (C3HC4-type RING finger) fa... 29 2.4
At3g28770.1 68416.m03591 expressed protein 29 2.4
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 29 3.1
At2g10350.1 68415.m01087 Ulp1 protease family protein similar to... 29 3.1
At1g51900.1 68414.m05850 hypothetical protein 29 3.1
At1g23230.1 68414.m02906 expressed protein 29 3.1
At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative si... 29 4.1
At4g11780.1 68417.m01877 hypothetical protein 29 4.1
At3g45530.1 68416.m04917 DC1 domain-containing protein contains ... 29 4.1
At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containi... 29 4.1
At5g60500.1 68418.m07587 undecaprenyl pyrophosphate synthetase f... 28 5.5
At3g57780.1 68416.m06436 expressed protein 28 5.5
At5g42490.1 68418.m05172 kinesin motor family protein contains P... 28 7.2
At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA methyltran... 28 7.2
At2g02720.1 68415.m00214 pectate lyase family protein similar to... 28 7.2
At1g49980.1 68414.m05609 UMUC-like DNA repair family protein low... 27 9.6
>At3g10180.1 68416.m01219 kinesin motor protein-related similar to
centromere protein E GB:4502781 [Homo sapiens]
Length = 1348
Score = 35.1 bits (77), Expect = 0.048
Identities = 25/83 (30%), Positives = 38/83 (45%)
Frame = +1
Query: 214 RRH*EDPEVFIRRYEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKL 393
++H ++ +E S L H E EK+ + + KEK E TKL
Sbjct: 776 QKHETSLKMLSEHHESERSDLLSHIECLEKD-IGSLSSSSLAKEKENLRKDFEK-TKTKL 833
Query: 394 KHTETCEKNSLPTKDVIEQEKSA 462
K TE+ KNS+ K +E EK++
Sbjct: 834 KDTESKLKNSMQDKTKLEAEKAS 856
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 34.3 bits (75), Expect = 0.083
Identities = 32/131 (24%), Positives = 52/131 (39%), Gaps = 4/131 (3%)
Frame = +3
Query: 3 PTESLSARIFYPLPHQKYIDSEWPAP*VTLPP*KT----SPRSPQT*RVSSKASTPAVSV 170
P+ ++ R+ P P + + S PA + PP + SP ++ +P+ S
Sbjct: 452 PSPPVAQRLPSPPPRRAGLPSPPPAQRLPSPPPRRAGLPSPMRIGGSHAANHLESPSPSS 511
Query: 171 TSTPMKRLCFRLLKTSPLRRPRSLYSTVREV*FEPAEAHRDSGEEPASGQRRYRSGEGKE 350
S P ++ ++L + P+RR RSL V H G G GK
Sbjct: 512 LSPPGRK---KVLPSPPVRRRRSLTPDEERVSLSQGGRHTSPSHIKQDGSMSPVRGRGKS 568
Query: 351 QIPERHRELRS 383
RH++ RS
Sbjct: 569 SPSSRHQKARS 579
>At4g36520.1 68417.m05185 trichohyalin-related low similarity to
SP|Q07283 Trichohyalin {Homo sapiens}
Length = 1400
Score = 31.1 bits (67), Expect = 0.78
Identities = 18/78 (23%), Positives = 34/78 (43%), Gaps = 1/78 (1%)
Frame = +1
Query: 274 QLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFD-PTKLKHTETCEKNSLPTKDVIEQ 450
Q+K + +E+N K+ +E + + K +E + +LK T E+N ++ IE
Sbjct: 778 QIKERQEREENERRAKEVLEQAENERKLKEALEQKENERRLKETREKEENKKKLREAIEL 837
Query: 451 EKSAXXXXXXXXXANVSR 504
E+ A + R
Sbjct: 838 EEKEKRLIEAFERAEIER 855
Score = 28.7 bits (61), Expect = 4.1
Identities = 16/68 (23%), Positives = 36/68 (52%), Gaps = 1/68 (1%)
Frame = +1
Query: 256 EKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFD-PTKLKHTETCEKNSLPT 432
+K + +LK T +E+N ++AIE E+++ + + E + +LK E+ +
Sbjct: 811 QKENERRLKETREKEENKKKLREAIELEEKEKRLIEAFERAEIERRLKEDLEQEEMRMRL 870
Query: 433 KDVIEQEK 456
++ E+E+
Sbjct: 871 QEAKERER 878
>At3g47180.1 68416.m05123 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 210
Score = 29.5 bits (63), Expect = 2.4
Identities = 16/60 (26%), Positives = 30/60 (50%)
Frame = +1
Query: 226 EDPEVFIRRYEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTE 405
++ E+ YE FDS+ E + L D+++ +E++ FL + DP +L + E
Sbjct: 62 DEEEINENYYEYFDSNGFGVDEDEINEFLEDQESNSNLEEEDDFLEEEDEIDPDQLSYEE 121
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 29.5 bits (63), Expect = 2.4
Identities = 20/66 (30%), Positives = 31/66 (46%), Gaps = 1/66 (1%)
Frame = +1
Query: 244 IRRYEKFDSSQLKHTETQEKNPLPD-KDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKN 420
+R E+ K +E EK + KDA E + NK L+ EN D K + E +++
Sbjct: 768 VRNKEENVQGNKKESEKVEKGEKKESKDAKSVETKDNKKLSSTENRDEAKERSGEDNKED 827
Query: 421 SLPTKD 438
+KD
Sbjct: 828 KEESKD 833
>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam
domain PF04931: DNA polymerase V
Length = 1306
Score = 29.1 bits (62), Expect = 3.1
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +1
Query: 286 TETQEKNPLPDKDAIEAEKEKNKFLNGIENFD 381
TE E + LPD ++ +K K + +N + N+D
Sbjct: 12 TELVENDNLPDSSIVKKKKSKTEKMNTVANYD 43
>At2g10350.1 68415.m01087 Ulp1 protease family protein similar to
At5g28170, At1g35110, At1g44880, At3g42530, At4g19320,
At5g36020, At4g03970, At3g43010 ; contains Pfam profile
PF02902: Ulp1 protease family, C-terminal catalytic
domain
Length = 1110
Score = 29.1 bits (62), Expect = 3.1
Identities = 20/49 (40%), Positives = 30/49 (61%), Gaps = 5/49 (10%)
Frame = +2
Query: 581 YNGNTAWAMAAYSNPGFYSNP-NDK-SRLTPI---NSEWTP*TDRITDN 712
++G+ AM YS+P + +N +DK S +P NSE TP D+ITD+
Sbjct: 742 FDGDPMKAMVIYSHPIYDTNSKHDKQSESSPTHSANSELTPTEDQITDS 790
>At1g51900.1 68414.m05850 hypothetical protein
Length = 774
Score = 29.1 bits (62), Expect = 3.1
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = +1
Query: 229 DPEVFIRRYEKFDSSQLKHTETQEKNPLPDKDAIEAEK 342
DP+++IR YE+ + K + T + + + D+IE K
Sbjct: 362 DPDIYIRSYEESPNEVYKFSLTDLEEEIMENDSIEGVK 399
>At1g23230.1 68414.m02906 expressed protein
Length = 1615
Score = 29.1 bits (62), Expect = 3.1
Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = -1
Query: 436 PLWGASSSRTFPCASA*WDRSSRC-RSGICSFPSP 335
PL+G + + P S W+R+ RC R I + PSP
Sbjct: 393 PLYGEDLAMSIPKGSLDWERAVRCIRHAIRTTPSP 427
>At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative
similar to sucrose-phosphate synthase isoform 1, Citrus
unshiu, PIR:S72648
Length = 894
Score = 28.7 bits (61), Expect = 4.1
Identities = 23/74 (31%), Positives = 36/74 (48%)
Frame = +2
Query: 8 RVAECTNLLSPSSSKIY*FRMACSVSDTPSLKDLPKVATDLKSQLEGFNTSCLRDVDTNE 187
R+A C P ++ F + S S + SL+D+ ++ +LK L+G + VDTN
Sbjct: 513 RIASCKQR-HPKWQRVE-FENSDSDSPSDSLRDINDISLNLKLSLDGEKSGSNNGVDTN- 569
Query: 188 KIVLPSAEDVATEK 229
AED A E+
Sbjct: 570 ----LDAEDRAAER 579
>At4g11780.1 68417.m01877 hypothetical protein
Length = 473
Score = 28.7 bits (61), Expect = 4.1
Identities = 15/47 (31%), Positives = 27/47 (57%)
Frame = -3
Query: 542 RSDLHQY*NTAN*RDTFASNCYKVVVQADFSCSMTSFVGSEFFSHVS 402
+SDL +T + + TF+++ + V +F +S GSEFF++ S
Sbjct: 170 QSDLFSQISTTDDKITFSTSAAAITVVEEFISGDSSSYGSEFFTNSS 216
>At3g45530.1 68416.m04917 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 692
Score = 28.7 bits (61), Expect = 4.1
Identities = 14/43 (32%), Positives = 21/43 (48%), Gaps = 2/43 (4%)
Frame = -3
Query: 482 CYKVVVQADFSCSMT--SFVGSEFFSHVSVCFSLVGSKFSMPF 360
C++ ++ DF C F F+S V F L+ S +MPF
Sbjct: 438 CFESIIDDDFLCRACYQMFCEGFFYSSKGVNFDLICSSITMPF 480
>At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containing
protein contains Pfam profile PF01388: ARID/BRIGHT DNA
binding domain
Length = 786
Score = 28.7 bits (61), Expect = 4.1
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 3/58 (5%)
Frame = +2
Query: 77 SVSDTPSLKDLPKVATDLKSQLEGF---NTSCLRDVDTNEKIVLPSAEDVATEKTQKS 241
S+ + + DLPK+ + SQ E + S +DT E ++ P+AED E S
Sbjct: 78 SLEEVTNADDLPKIDDEKNSQFETSPHPSPSPSVALDTEEGLINPTAEDTVEENIVSS 135
>At5g60500.1 68418.m07587 undecaprenyl pyrophosphate synthetase
family protein / UPP synthetase family protein contains
putative undecaprenyl diphosphate synthase domain
[PF01255]; similar to S. cerevisiae dehydrodolichyl
diphosphate synthetase (DEDOL-PP
synthase)(Rer2)[SP|P35196], a cis-prenyltransferase
Length = 271
Score = 28.3 bits (60), Expect = 5.5
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = -1
Query: 229 LLSGDVFSRRKHNLFIGVDVTETAGVEAFELTLQVCGDLG 110
++ G+ +KHNL IG+D AG + + LQ C ++G
Sbjct: 41 IMDGNRRFAKKHNL-IGLDAGHRAGFISVKYILQYCKEIG 79
>At3g57780.1 68416.m06436 expressed protein
Length = 670
Score = 28.3 bits (60), Expect = 5.5
Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +1
Query: 265 DSSQLKHTETQEK-NPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCE 414
DSS+ TE +E N L D E EKE++K L+ I + + ETCE
Sbjct: 72 DSSE--KTEKEETINGLACDDEDEEEKEESKELDAIAHEKTDSVSSPETCE 120
>At5g42490.1 68418.m05172 kinesin motor family protein contains Pfam
domain, PF00225: Kinesin motor domain
Length = 1087
Score = 27.9 bits (59), Expect = 7.2
Identities = 20/64 (31%), Positives = 32/64 (50%)
Frame = +1
Query: 256 EKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNSLPTK 435
E +S + + E Q KN ++ + K+ + LN EN ++ + TE CE NSLP
Sbjct: 564 EAQESQESVNEEEQMKNE--ERKMSPSTKQAEQCLNKEENAQ-SEQQSTEDCELNSLPIN 620
Query: 436 DVIE 447
+ E
Sbjct: 621 NQSE 624
>At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA
methyltransferase family protein similar to
N2,N2-dimethylguanosine tRNA methyltransferase [Homo
sapiens] GI:11066198; contains Pfam profile PF02005:
N2,N2-dimethylguanosine tRNA methyltransferase
Length = 599
Score = 27.9 bits (59), Expect = 7.2
Identities = 16/49 (32%), Positives = 25/49 (51%)
Frame = +1
Query: 235 EVFIRRYEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFD 381
E +R ++ ++ K T K + +KDA EA KE+ NG+ N D
Sbjct: 45 EFLSKRKQEHEAKSSKRTRPASK--VIEKDASEASKEETPSENGMNNGD 91
>At2g02720.1 68415.m00214 pectate lyase family protein similar to
pectate lyase P59 SP:P15722 from [Lycopersicon
esculentum]
Length = 455
Score = 27.9 bits (59), Expect = 7.2
Identities = 16/58 (27%), Positives = 23/58 (39%)
Frame = +1
Query: 280 KHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNSLPTKDVIEQE 453
K +E P P+ + + EK+ FLNG K KN +P K I +
Sbjct: 375 KQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFAIRPQ 432
>At1g49980.1 68414.m05609 UMUC-like DNA repair family protein low
similarity to DNA polymerase kappa [Mus musculus]
GI:14279087; contains Pfam profile PF00817:
ImpB/MucB/SamB family
Length = 785
Score = 27.5 bits (58), Expect = 9.6
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = -1
Query: 232 GLLSGDVFSRRKHNLFIGVDVTETAGVEAFELTLQVCGDLGE 107
GL G++ + +++ +T +AGV A L +VC D+ +
Sbjct: 214 GLSGGEIAEELRSSVYSETGLTCSAGVAANRLLAKVCSDINK 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,578,278
Number of Sequences: 28952
Number of extensions: 334573
Number of successful extensions: 1156
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1061
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1141
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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