Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00265
(755 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr... 145 3e-37
AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic ac... 23 4.1
AB047034-1|BAB64310.1| 1598|Apis mellifera mblk-1 protein. 22 5.4
DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated... 22 7.1
AF144379-1|AAD34586.1| 543|Apis mellifera glutamate transporter... 21 9.4
>AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor
protein.
Length = 1370
Score = 145 bits (352), Expect = 3e-37
Identities = 56/80 (70%), Positives = 66/80 (82%)
Frame = +3
Query: 15 KTTDFLCKYETHCFAICHCCDYDACDCEMTCPHNCTCYHDPLWNTNVVDCSGQSSMEIPQ 194
++ FLC+YE HCFA+CHCCD+DACDCEMTCP C CY+D WNTN VDCSG EIP+
Sbjct: 731 RSEQFLCRYEAHCFALCHCCDFDACDCEMTCPAGCKCYNDRTWNTNAVDCSGLGVEEIPR 790
Query: 195 KIPMDATEVFLDGNNIRELQ 254
+IPMDATEV+LDGN +RELQ
Sbjct: 791 RIPMDATEVYLDGNVLRELQ 810
Score = 119 bits (286), Expect = 3e-29
Identities = 51/82 (62%), Positives = 72/82 (87%)
Frame = +2
Query: 248 IADHVFIGRQKMRTLYVNNSNVDSIQNRTFSGLNSLQILHLGNNKLKELKGYEFQQLNNL 427
+ +HVFIGR+ MR LYVN S ++SIQNRTF+GLN+LQILHL +N+++ELKG+EF++L++L
Sbjct: 809 LQNHVFIGRKNMRVLYVNGSGIESIQNRTFNGLNNLQILHLEDNRIRELKGFEFERLSHL 868
Query: 428 KELFLQNNLISHIANISFLSLK 493
+EL+LQNNLI I N++FL L+
Sbjct: 869 RELYLQNNLIGFIGNLTFLPLR 890
Score = 110 bits (264), Expect = 2e-26
Identities = 46/91 (50%), Positives = 67/91 (73%), Gaps = 1/91 (1%)
Frame = +3
Query: 486 LLSPRVVTLDGNRLVDFGVWSFSNNINLKALSLGNNLWSCKCRYLQELTAFLAENAQKII 665
L S ++ L GNRLV F VW + N L LSLG+N WSC+C++LQEL++++++NA K++
Sbjct: 889 LRSLEILRLSGNRLVTFPVWQVTLNARLVELSLGSNPWSCRCKFLQELSSWVSDNAHKVV 948
Query: 666 DITDVWC-WNGDAKPPQKKELNLNGTACSDY 755
D +DVWC + GDA+P ++ LN+N T CSDY
Sbjct: 949 DASDVWCYYGGDARPAYRRRLNVNETVCSDY 979
Score = 40.3 bits (90), Expect = 2e-05
Identities = 16/64 (25%), Positives = 37/64 (57%)
Frame = +2
Query: 290 LYVNNSNVDSIQNRTFSGLNSLQILHLGNNKLKELKGYEFQQLNNLKELFLQNNLISHIA 469
+Y++ + + +QN F G ++++L++ + ++ ++ F LNNL+ L L++N I +
Sbjct: 799 VYLDGNVLRELQNHVFIGRKNMRVLYVNGSGIESIQNRTFNGLNNLQILHLEDNRIRELK 858
Query: 470 NISF 481
F
Sbjct: 859 GFEF 862
Score = 35.9 bits (79), Expect = 4e-04
Identities = 21/57 (36%), Positives = 30/57 (52%)
Frame = +2
Query: 281 MRTLYVNNSNVDSIQNRTFSGLNSLQILHLGNNKLKELKGYEFQQLNNLKELFLQNN 451
+R LY+ N+ + I N TF L SL+IL L N+L ++ L EL L +N
Sbjct: 868 LRELYLQNNLIGFIGNLTFLPLRSLEILRLSGNRLVTFPVWQVTLNARLVELSLGSN 924
Score = 35.1 bits (77), Expect = 7e-04
Identities = 15/49 (30%), Positives = 31/49 (63%)
Frame = +2
Query: 248 IADHVFIGRQKMRTLYVNNSNVDSIQNRTFSGLNSLQILHLGNNKLKEL 394
+A F+G +++ TL + SNV ++ + L++LQ L+L N+L+++
Sbjct: 138 LAPDSFLGLRELHTLEIVESNVQALPVNSLCSLDNLQTLNLTENRLRDI 186
Score = 35.1 bits (77), Expect = 7e-04
Identities = 21/68 (30%), Positives = 34/68 (50%)
Frame = +2
Query: 248 IADHVFIGRQKMRTLYVNNSNVDSIQNRTFSGLNSLQILHLGNNKLKELKGYEFQQLNNL 427
+ + F+G ++ L ++ + + I R F L LQIL L NN + ++ F L NL
Sbjct: 326 VDETTFLGLIRLIVLNLSYNMLTHIDARMFKDLFFLQILDLRNNSIDRIESNAFLPLYNL 385
Query: 428 KELFLQNN 451
L L +N
Sbjct: 386 HTLELSDN 393
Score = 34.7 bits (76), Expect = 0.001
Identities = 18/73 (24%), Positives = 38/73 (52%)
Frame = +2
Query: 248 IADHVFIGRQKMRTLYVNNSNVDSIQNRTFSGLNSLQILHLGNNKLKELKGYEFQQLNNL 427
I + F+ + TL ++++ + ++ + F+GL L L L N + + F+ ++L
Sbjct: 374 IESNAFLPLYNLHTLELSDNKLRTVGAQLFNGLFVLNRLTLSGNAIASIDPLAFRNCSDL 433
Query: 428 KELFLQNNLISHI 466
KEL L N ++ +
Sbjct: 434 KELDLSGNELTSV 446
Score = 34.3 bits (75), Expect = 0.001
Identities = 23/83 (27%), Positives = 38/83 (45%), Gaps = 2/83 (2%)
Frame = +2
Query: 248 IADHVFIGRQKMRTLYVNNSNVDSIQNRTFSGLNSLQILHLGNNKLKELKGYEFQQLNNL 427
IA G +RT + +++DS+ F+ L+ +HL N L++L F +L L
Sbjct: 252 IAGDALTGLTVLRTFNASYNSLDSLPEGLFASTRDLREIHLAYNGLRDLPKGIFTRLEQL 311
Query: 428 KELFLQNNLI--SHIANISFLSL 490
L L N + + +FL L
Sbjct: 312 LVLNLAGNRLGSDRVDETTFLGL 334
Score = 33.1 bits (72), Expect = 0.003
Identities = 17/71 (23%), Positives = 37/71 (52%)
Frame = +2
Query: 263 FIGRQKMRTLYVNNSNVDSIQNRTFSGLNSLQILHLGNNKLKELKGYEFQQLNNLKELFL 442
F ++ L + +++ ++ L +LQIL+L NK++ ++ Y F++ L+ + L
Sbjct: 474 FRNLDQLTGLRLIGNDIGNLSRGMLWDLPNLQILNLARNKVQHVERYAFERNMRLEAIRL 533
Query: 443 QNNLISHIANI 475
N +S I +
Sbjct: 534 DGNFLSDINGV 544
Score = 24.2 bits (50), Expect = 1.3
Identities = 17/82 (20%), Positives = 39/82 (47%)
Frame = +2
Query: 248 IADHVFIGRQKMRTLYVNNSNVDSIQNRTFSGLNSLQILHLGNNKLKELKGYEFQQLNNL 427
+ ++ I K++TL +++ + + S +S+++L + NN + ++ F NL
Sbjct: 586 LGNYYKIRDSKVKTLDASHNRITELS--PLSVPDSVELLFINNNYINLVRPNTFTDKVNL 643
Query: 428 KELFLQNNLISHIANISFLSLK 493
+ + N+I + S L K
Sbjct: 644 TRVDMYANMIETMELTSLLLTK 665
Score = 21.4 bits (43), Expect = 9.4
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = +3
Query: 498 RVVTLDGNRLVDFGVWSFSNNINLKALSLGNNL 596
+++ L+ NR+ + + F +L+ L L NNL
Sbjct: 845 QILHLEDNRIRELKGFEFERLSHLRELYLQNNL 877
Score = 21.4 bits (43), Expect = 9.4
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = -2
Query: 625 SCR*RHLQDHKLFPNDNAFKFI 560
SCR + LQ+ + +DNA K +
Sbjct: 927 SCRCKFLQELSSWVSDNAHKVV 948
>AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic
acetylcholine receptorApisa2 subunit protein.
Length = 541
Score = 22.6 bits (46), Expect = 4.1
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1
Query: 7 LTLRRQTFFVSMRLIV 54
+TLRR+T F ++ LIV
Sbjct: 229 ITLRRKTLFYTVNLIV 244
>AB047034-1|BAB64310.1| 1598|Apis mellifera mblk-1 protein.
Length = 1598
Score = 22.2 bits (45), Expect = 5.4
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = -2
Query: 547 LHTPKSTSLLPSRVTT 500
LH P S++ LP+ +TT
Sbjct: 94 LHPPASSTSLPATITT 109
>DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated
ion channel subunit protein.
Length = 510
Score = 21.8 bits (44), Expect = 7.1
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +3
Query: 594 LWSCKCRYLQELTAFLA 644
L SCKC Y+Q + +A
Sbjct: 180 LGSCKCIYVQSINLCMA 196
>AF144379-1|AAD34586.1| 543|Apis mellifera glutamate transporter
Am-EAAT protein.
Length = 543
Score = 21.4 bits (43), Expect = 9.4
Identities = 7/21 (33%), Positives = 14/21 (66%)
Frame = +3
Query: 207 DATEVFLDGNNIRELQITCLS 269
+AT V+ DG N+ + + C++
Sbjct: 229 EATLVYKDGTNVMGMIVFCIT 249
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 202,470
Number of Sequences: 438
Number of extensions: 4741
Number of successful extensions: 22
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 23753925
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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