BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00258
(769 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pomb... 28 1.3
SPBC409.09c |mis13|cnl1|kinetochore protein Mis13|Schizosaccharo... 27 3.9
SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce... 26 5.2
SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2 |Schizosa... 26 5.2
SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomy... 26 6.8
SPBC16H5.12c |||conserved fungal protein|Schizosaccharomyces pom... 26 6.8
SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 9.0
>SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 910
Score = 28.3 bits (60), Expect = 1.3
Identities = 16/59 (27%), Positives = 22/59 (37%)
Frame = +2
Query: 518 RFLSAARHALGSV*TSFAFALNSFSHTTSHVDYYQFDYFIR*TYTVLESIYWKLDNRAC 694
RF+ ARH + T F LN F S Y D F+ Y + + + C
Sbjct: 182 RFIELARHENPKIRTDALFCLNQFVLIQSQSLYAHIDTFLETCYALATDVSPNVRKNVC 240
>SPBC409.09c |mis13|cnl1|kinetochore protein
Mis13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 329
Score = 26.6 bits (56), Expect = 3.9
Identities = 11/23 (47%), Positives = 13/23 (56%)
Frame = -2
Query: 588 NELRAKANDVHTEPRAWRAADKN 520
+EL AK +H E AWRA N
Sbjct: 186 DELSAKLTQLHNEEAAWRAVAAN 208
>SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 964
Score = 26.2 bits (55), Expect = 5.2
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +2
Query: 590 SHTTSHVDYYQFDYFIR 640
SH +DYY +DYF++
Sbjct: 32 SHPNESLDYYIYDYFVK 48
>SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 459
Score = 26.2 bits (55), Expect = 5.2
Identities = 17/64 (26%), Positives = 29/64 (45%)
Frame = +3
Query: 492 DSLVQHWVIGSCPPHATPSVPCERHLLSPSIHFRTLHRMWIIINLIILFDEPIPFWNRFI 671
DS V H+ + C H + ++HL HF L + ++ + DE + FW +
Sbjct: 282 DSFVSHFPL--CAKHLHEVLKRDKHLR----HFGRLQYGLFLKDIGLSVDEALVFWRKSF 335
Query: 672 GNLT 683
N+T
Sbjct: 336 TNVT 339
>SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1201
Score = 25.8 bits (54), Expect = 6.8
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +3
Query: 645 PIPFWNRFIGNLTIELATSKMIEMLNLPTCLPSVE 749
PI ++ + +IE+ TSK+ +L L LP+VE
Sbjct: 646 PITDSHKILFKKSIEVITSKIRRLLKLDVYLPTVE 680
>SPBC16H5.12c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 682
Score = 25.8 bits (54), Expect = 6.8
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -1
Query: 121 F*IFVYIIWCVHDNVHILNKRRILKHNI 38
F + VY++W ++VH+L K R +++I
Sbjct: 98 FSVPVYVVWGACEDVHVLEKFRSGEYSI 125
>SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 25.4 bits (53), Expect = 9.0
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +3
Query: 390 YVYNSVFTCINL*IPKRSNRY*PSVLSCGHLW*SDSLVQ 506
Y ++SVFTC + + + P ++SCGH+ +SL Q
Sbjct: 333 YHFHSVFTC-PVSKEQATEENPPMMMSCGHVIVKESLRQ 370
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,999,085
Number of Sequences: 5004
Number of extensions: 60364
Number of successful extensions: 148
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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