Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00255
(462 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B) 100 4e-22
At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B) 100 4e-22
At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A) ribo... 100 4e-22
At1g53920.1 68414.m06138 GDSL-motif lipase/hydrolase family prot... 30 0.88
At5g41840.1 68418.m05095 F-box family protein contains F-box dom... 29 1.5
At3g59190.1 68416.m06599 F-box family protein contains F-box dom... 28 3.5
At5g44490.1 68418.m05451 F-box family protein contains F-box dom... 27 4.7
At4g07670.1 68417.m01203 protease-associated (PA) domain-contain... 27 4.7
At3g59160.1 68416.m06596 F-box family protein contains F-box dom... 27 4.7
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 27 6.2
At3g59170.1 68416.m06597 F-box family protein contains F-box dom... 27 6.2
At4g02020.1 68417.m00272 zeste-like protein 1 (EZA1) identical t... 27 8.2
At3g30820.1 68416.m03953 hypothetical protein 27 8.2
>At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B)
Length = 133
Score = 100 bits (240), Expect = 4e-22
Identities = 45/72 (62%), Positives = 58/72 (80%)
Frame = +2
Query: 38 MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYG 217
MA+ P+ +VKKR+ +FIR QSDR +K +WR+P+GID+RVRR+FKG LMPN+GYG
Sbjct: 1 MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYG 59
Query: 218 SNKKTRHMLPNG 253
S+KKTRH LPNG
Sbjct: 60 SDKKTRHYLPNG 71
Score = 92.3 bits (219), Expect = 1e-19
Identities = 42/67 (62%), Positives = 50/67 (74%)
Frame = +1
Query: 238 YAPKWIRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIRVTNAAAR 417
Y P +K +VHN ELE+LMM NR YCAEIAH +S+KKRK IVERA QL + V+N R
Sbjct: 67 YLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTKKRKAIVERASQLDVVVSNKLGR 126
Query: 418 LRSQENE 438
LRSQE+E
Sbjct: 127 LRSQEDE 133
>At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B)
Length = 133
Score = 100 bits (240), Expect = 4e-22
Identities = 45/72 (62%), Positives = 58/72 (80%)
Frame = +2
Query: 38 MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYG 217
MA+ P+ +VKKR+ +FIR QSDR +K +WR+P+GID+RVRR+FKG LMPN+GYG
Sbjct: 1 MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYG 59
Query: 218 SNKKTRHMLPNG 253
S+KKTRH LPNG
Sbjct: 60 SDKKTRHYLPNG 71
Score = 92.3 bits (219), Expect = 1e-19
Identities = 42/67 (62%), Positives = 50/67 (74%)
Frame = +1
Query: 238 YAPKWIRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIRVTNAAAR 417
Y P +K +VHN ELE+LMM NR YCAEIAH +S+KKRK IVERA QL + V+N R
Sbjct: 67 YLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTKKRKAIVERASQLDVVVSNKLGR 126
Query: 418 LRSQENE 438
LRSQE+E
Sbjct: 127 LRSQEDE 133
>At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A)
ribosomal protein L32, human, PIR1:R5HU32
Length = 133
Score = 100 bits (240), Expect = 4e-22
Identities = 45/72 (62%), Positives = 58/72 (80%)
Frame = +2
Query: 38 MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYG 217
MA+ P+ +VKKR+ +FIR QSDR +K +WR+P+GID+RVRR+FKG LMPN+GYG
Sbjct: 1 MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYG 59
Query: 218 SNKKTRHMLPNG 253
S+KKTRH LPNG
Sbjct: 60 SDKKTRHYLPNG 71
Score = 95.5 bits (227), Expect = 2e-20
Identities = 45/67 (67%), Positives = 51/67 (76%)
Frame = +1
Query: 238 YAPKWIRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIRVTNAAAR 417
Y P +K +VHN ELE+LMM NR YCAEIAH VS+KKRK IVERA QL + VTN AR
Sbjct: 67 YLPNGFKKFVVHNTSELELLMMHNRTYCAEIAHNVSTKKRKAIVERASQLDVVVTNRLAR 126
Query: 418 LRSQENE 438
LRSQE+E
Sbjct: 127 LRSQEDE 133
>At1g53920.1 68414.m06138 GDSL-motif lipase/hydrolase family protein
similar to Anther-specific proline-rich proteins
SP|P40603 SP|P40602 from {Arabidopsis thaliana};
contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase
Length = 385
Score = 29.9 bits (64), Expect = 0.88
Identities = 19/55 (34%), Positives = 27/55 (49%)
Frame = +2
Query: 95 IRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLPNGSV 259
+R Q D Y K++R WR G + +R + YL I GSN + L N S+
Sbjct: 146 LRTQLDHYKKVERLWRTNFGKEESKKRISRAVYL---ISIGSNDYSSIFLTNQSL 197
>At5g41840.1 68418.m05095 F-box family protein contains F-box domain
Pfam:PF00646
Length = 540
Score = 29.1 bits (62), Expect = 1.5
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = +1
Query: 229 DPSYAPKWIRKVLVHNVKELEILMMQNRKYC 321
D S+ +WI KVL V ++++ + +R YC
Sbjct: 110 DQSWVLEWIPKVLKRGVLDIDLHITSSRNYC 140
>At3g59190.1 68416.m06599 F-box family protein contains F-box domain
Pfam:PF00646
Length = 388
Score = 27.9 bits (59), Expect = 3.5
Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = +1
Query: 175 AIQGSILDAQHRLRFQ-QEDPSYAPKWIRKVLVHNVKELEILMMQNRKY 318
A+QG+ + LR + DPS W+ KVL V+EL++ + +Y
Sbjct: 87 ALQGNAPLKKFSLRCKIGSDPSRVNGWVLKVLDRGVEELDLYIASEYEY 135
>At5g44490.1 68418.m05451 F-box family protein contains F-box domain
Pfam:PF00646
Length = 455
Score = 27.5 bits (58), Expect = 4.7
Identities = 11/41 (26%), Positives = 21/41 (51%)
Frame = +1
Query: 208 RLRFQQEDPSYAPKWIRKVLVHNVKELEILMMQNRKYCAEI 330
R + ++ S +WI V H +K L++ + +K C E+
Sbjct: 98 RKEHENDNKSCVTRWIGFVATHKLKHLDVECLLWKKKCLEV 138
>At4g07670.1 68417.m01203 protease-associated (PA) domain-containing
protein similar to PF02225: PA domain; similar to
N-acetylated-alpha-linked acidic dipeptidase II
(NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to
Glutamate carboxypeptidase II (Membrane glutamate
carboxypeptidase) (mGCP) (N-acetylated-alpha-linked
acidic dipeptidase I) (NAALADase I)
(Pteroylpoly-gamma-glutamate
carboxypeptidase)(Folylpoly-gamma-glutamate
carboxypeptidase) (FGCP) (Folate hydrolase 1)
(Prostate-specific membrane antigen homolog) (SP|O77564)
{Sus scrofa}
Length = 280
Score = 27.5 bits (58), Expect = 4.7
Identities = 11/37 (29%), Positives = 22/37 (59%)
Frame = +2
Query: 41 AIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPR 151
A+ P ++ + +K +++H + R DKL++ KPR
Sbjct: 194 AVDPNSGTAVLMEASKSYLQHIAQRLDKLQKRGWKPR 230
>At3g59160.1 68416.m06596 F-box family protein contains F-box domain
Pfam:PF00646
Length = 464
Score = 27.5 bits (58), Expect = 4.7
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = +1
Query: 229 DPSYAPKWIRKVLVHNVKELEILMMQN 309
DP WI KVLV V +L +++ N
Sbjct: 127 DPDCVAPWIHKVLVRGVSDLHLVIDMN 153
>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam domain
PF04931: DNA polymerase V
Length = 1306
Score = 27.1 bits (57), Expect = 6.2
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +1
Query: 259 KVLVHNVKELEILMMQNRKYCAEIAHGVSSKK 354
K LV N+ E ++ Q RK+C I VSS K
Sbjct: 1242 KELVGNMPEAKVRRAQVRKFCGRIFQMVSSLK 1273
>At3g59170.1 68416.m06597 F-box family protein contains F-box domain
Pfam:PF00646
Length = 473
Score = 27.1 bits (57), Expect = 6.2
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = +1
Query: 229 DPSYAPKWIRKVLVHNVKELEILMMQN 309
DP WI KVLV V +L +++ N
Sbjct: 121 DPDSVVPWIHKVLVRGVSDLHLVVDMN 147
>At4g02020.1 68417.m00272 zeste-like protein 1 (EZA1) identical to
enhancer of zeste-like protein 1(EZA1) (GI:4185507)
[Arabidopsis thaliana]; similar to polycomb group
[Arabidopsis thaliana] GI:1903019 (curly leaf); contains
Pfam profile PF00856: SET domain
Length = 856
Score = 26.6 bits (56), Expect = 8.2
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +1
Query: 25 RNIQDGYKTCLQADNRQKEDE 87
RN+ G KTCL N +E+E
Sbjct: 517 RNLLSGLKTCLDVSNYMRENE 537
>At3g30820.1 68416.m03953 hypothetical protein
Length = 405
Score = 26.6 bits (56), Expect = 8.2
Identities = 19/58 (32%), Positives = 27/58 (46%), Gaps = 4/58 (6%)
Frame = +3
Query: 276 C*RAGNLDDAKQEVLRR---DR-SRCLFEEAEADRGKSPAAQHQSNECGRSPPVPGER 437
C R G +DD +E + DR + L A A ++P Q + GRS PGE+
Sbjct: 338 CTRGGGVDDGDREHRSKATEDRHAERLVPAAGASTSQTPPEQSLGHRLGRSRRQPGEQ 395
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,753,084
Number of Sequences: 28952
Number of extensions: 194839
Number of successful extensions: 681
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 681
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 772134480
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -