Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00254
         (810 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC7D4.10 |vma13||V-type ATPase subunit H|Schizosaccharomyces p...   109   5e-25
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ...    33   0.048
SPBC115.03 ||SPBC839.18c|gfo/idh/mocA family oxidoreductase |Sch...    30   0.45 
SPAC3H1.05 |||CAAX prenyl protease |Schizosaccharomyces pombe|ch...    29   0.78 
SPBC24C6.07 |cdc14||SIN component Cdc14|Schizosaccharomyces pomb...    27   2.4  
SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizos...    27   3.2  
SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr...    27   4.2  
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb...    26   5.5  
SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosac...    26   5.5  
SPBP23A10.03c |||ACN9 family mitochondrial protein|Schizosacchar...    26   7.3  
SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe...    25   9.6  
SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr 2...    25   9.6  
SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosacc...    25   9.6  

>SPAC7D4.10 |vma13||V-type ATPase subunit H|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 450

 Score =  109 bits (262), Expect = 5e-25
 Identities = 60/173 (34%), Positives = 98/173 (56%), Gaps = 1/173 (0%)
 Frame = +3

Query: 255 AVFRNLIEKPEDQQVAKEHCIAMVQCKVLKQLSILEQKRSDDEDIMNDVEYLNERLQASV 434
           A+  NLI+K       K+    M+   V K + +L++++  DEDI N ++++   L  S 
Sbjct: 271 AILVNLIDK-----APKDTISTMLLEHVDKAVQLLQKRKWADEDITNYLDFITSTLDESS 325

Query: 435 QDLSSFDQYATEVKSGAWN-GPRYTNGQVLARKRGSFERTCQELLRTLVHLLEKSRDPVV 611
           + LS+FD Y +E+ +G  +  P + +     +           LL+ L H+++ + D   
Sbjct: 326 KHLSTFDMYKSELDTGILHWSPSHRSEDFWHQNAKRLNEDNYALLKKLFHIVQYNEDNTS 385

Query: 612 LAVACYDIGEYVRHYPRGKHIIEQLGGKQRVMYLLSHDDPNVRYEALLAVRNL 770
           LAVAC+D+G Y+R YP G+ +I + G KQR+M L+SH DP VR+EAL  V+ L
Sbjct: 386 LAVACHDLGAYIRSYPEGRSLIIKYGAKQRIMDLMSHPDPEVRFEALSTVQLL 438



 Score = 55.2 bits (127), Expect = 1e-08
 Identities = 37/106 (34%), Positives = 60/106 (56%), Gaps = 3/106 (2%)
 Frame = +1

Query: 4   RCLQMMLRIDEYRFAFLSVDGIS-TLLSILASRV-NFQVQYQLVFCLWVLTFNPLLAEKM 177
           +CL  +L +  +R+A  + +  S  L  +L + + + Q+QY  +FC W LTF   +A+ +
Sbjct: 184 QCLNGVLTLKAHRYALWAENTCSFRLAELLRNSIGDTQLQYYSLFCFWQLTFESHIAQDI 243

Query: 178 NK-FNVIPILADILSDSVKEKVTRIVLQCSGI*LKNLKINRLPKST 312
           NK F++I +L  I+    K KV R+VL      L NL I++ PK T
Sbjct: 244 NKRFDLIKLLVQIIRSDTKTKVYRLVLAI----LVNL-IDKAPKDT 284


>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1044

 Score = 33.1 bits (72), Expect = 0.048
 Identities = 16/51 (31%), Positives = 32/51 (62%)
 Frame = +3

Query: 303 KEHCIAMVQCKVLKQLSILEQKRSDDEDIMNDVEYLNERLQASVQDLSSFD 455
           KE+ I+ ++ K+ ++ + L++ +S      N +E LNER+ A+  +LSS +
Sbjct: 797 KENIISDLKNKLSEESTRLQELQSQLNQDKNQIETLNERISAAADELSSME 847


>SPBC115.03 ||SPBC839.18c|gfo/idh/mocA family oxidoreductase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 368

 Score = 29.9 bits (64), Expect = 0.45
 Identities = 20/58 (34%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
 Frame = +3

Query: 348 LSILEQKRSDDEDIMNDVEYLNERLQASVQDLSSFDQYATEVKSGAW-NGPRYTNGQV 518
           L+I + +R D + +       + RL   V+  S FD+Y    KSG+W + PR  NG V
Sbjct: 122 LAIYQNRRWDGDFLTAKKIIESGRLGQVVEFESHFDRYRLGRKSGSWKDEPRPGNGMV 179


>SPAC3H1.05 |||CAAX prenyl protease |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 474

 Score = 29.1 bits (62), Expect = 0.78
 Identities = 22/84 (26%), Positives = 39/84 (46%), Gaps = 2/84 (2%)
 Frame = +3

Query: 537 SFERTCQE-LLRTLVHLLEKSRDPVVLAVACYDIGEYVRHYPRGKHIIEQLGGKQRVMYL 713
           SF   C+  +L+ L+H+L+    P  + +A + IG+Y       +  +  L  ++    L
Sbjct: 24  SFANCCKMGILQHLMHILDIPGFPWKIVIAGFSIGKYAWDLYLRRRQVPYLLREKPPAIL 83

Query: 714 LSHDDPNVRYEALLAVRN-LWFTT 782
             H D     +AL   R+  WF+T
Sbjct: 84  AEHVDEKKYQKALSYARDKSWFST 107


>SPBC24C6.07 |cdc14||SIN component Cdc14|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 240

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 13/53 (24%), Positives = 25/53 (47%)
 Frame = +3

Query: 564 LRTLVHLLEKSRDPVVLAVACYDIGEYVRHYPRGKHIIEQLGGKQRVMYLLSH 722
           LR  +HLL+ S+  ++   A   +   +   P    + EQ+ G Q++  +  H
Sbjct: 118 LRLFIHLLQPSQPSMLQVAAMKTLVCIMADRPLAIRLFEQINGLQQICVVFKH 170


>SPBP35G2.05c |cki2||serine/threonine protein kinase
           Cki2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 435

 Score = 27.1 bits (57), Expect = 3.2
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = -2

Query: 746 FVTYIRIVVTQEVHDTLFTTKLFDDVFAA 660
           ++TY+R +   E  D  F  +LFDDV  A
Sbjct: 256 YMTYVRSLEFDEEPDYAFLQELFDDVLRA 284


>SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 539

 Score = 26.6 bits (56), Expect = 4.2
 Identities = 23/72 (31%), Positives = 28/72 (38%)
 Frame = +3

Query: 573 LVHLLEKSRDPVVLAVACYDIGEYVRHYPRGKHIIEQLGGKQRVMYLLSHDDPNVRYEAL 752
           LV LL  S    +   A   +G  V        II   G       LLSH   N+R EA 
Sbjct: 295 LVELLS-SPSVNIQTPALRSVGNIVTGTDAQTQIIIDCGALNAFPSLLSHQKENIRKEAC 353

Query: 753 LAVRNLWFTTGN 788
             + N+  T GN
Sbjct: 354 WTISNI--TAGN 363


>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1207

 Score = 26.2 bits (55), Expect = 5.5
 Identities = 15/62 (24%), Positives = 31/62 (50%)
 Frame = +3

Query: 339 LKQLSILEQKRSDDEDIMNDVEYLNERLQASVQDLSSFDQYATEVKSGAWNGPRYTNGQV 518
           L++++I+ +KR+D   +  +  YLNE +++ +  L     Y     +   NG      Q+
Sbjct: 723 LQKMAIIAEKRNDTLSLFQNSLYLNE-VESRINILEQAGMYPIAYLTAKSNGLEEKAQQI 781

Query: 519 LA 524
           L+
Sbjct: 782 LS 783


>SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit
           Vps23|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 487

 Score = 26.2 bits (55), Expect = 5.5
 Identities = 12/47 (25%), Positives = 22/47 (46%)
 Frame = +3

Query: 576 VHLLEKSRDPVVLAVACYDIGEYVRHYPRGKHIIEQLGGKQRVMYLL 716
           ++L   + D  +L  AC  +  Y    P    ++++ GGK +   LL
Sbjct: 65  IYLSTPAFDKTILTSACITVRSYYPSQPPIVQLLDEKGGKHKYTSLL 111


>SPBP23A10.03c |||ACN9 family mitochondrial
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 115

 Score = 25.8 bits (54), Expect = 7.3
 Identities = 9/27 (33%), Positives = 19/27 (70%)
 Frame = +3

Query: 441 LSSFDQYATEVKSGAWNGPRYTNGQVL 521
           LSS+++YA  +++ +W   +Y+N  +L
Sbjct: 64  LSSWERYADALENESWKQEKYSNTDLL 90


>SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 570

 Score = 25.4 bits (53), Expect = 9.6
 Identities = 9/23 (39%), Positives = 17/23 (73%)
 Frame = +3

Query: 558 ELLRTLVHLLEKSRDPVVLAVAC 626
           E++  ++ L+EKS++P +L  AC
Sbjct: 203 EVVSEILELIEKSKNPSILVDAC 225


>SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 803

 Score = 25.4 bits (53), Expect = 9.6
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = +3

Query: 318 AMVQCKVLKQLSILEQKRSDDEDIMN 395
           AM+   VLKQ+ ++  K+ DD D  N
Sbjct: 264 AMIPQDVLKQIRLILTKKEDDADAEN 289


>SPBC19C2.11c |||mitochondrial outer membrane protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 452

 Score = 25.4 bits (53), Expect = 9.6
 Identities = 12/36 (33%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
 Frame = -3

Query: 577 TRVRSSSWHV-RSNEPRFRARTWPFVYRGPFQAPLF 473
           T++R++   V +SN P F +R      R P   P+F
Sbjct: 86  TKIRANPLSVQKSNVPSFSSRNTMLASRAPLTVPMF 121


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,275,072
Number of Sequences: 5004
Number of extensions: 65715
Number of successful extensions: 215
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 211
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 213
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 394431430
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)