SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00250
         (513 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC2F12.15c |||palmitoyltransferase|Schizosaccharomyces pombe|c...    53   3e-08
SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces pom...    44   1e-05
SPBC13G1.07 |||palmitoyltransferase|Schizosaccharomyces pombe|ch...    44   1e-05
SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|ch...    41   1e-04
SPBC691.01 |||palmitoyltransferase |Schizosaccharomyces pombe|ch...    40   3e-04
SPAC4D7.12c |fet5||transcription factor Fet5|Schizosaccharomyces...    29   0.54 
SPCC306.06c |||ER membrane protein, BIG1 family |Schizosaccharom...    28   0.71 
SPBC2D10.04 |||arrestin Aly1 related|Schizosaccharomyces pombe|c...    28   0.71 
SPBC409.20c |psh3||ER chaperone SHR3 homologue Psh3|Schizosaccha...    28   0.94 
SPBP8B7.26 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    28   0.94 
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces...    27   1.6  
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa...    27   1.6  
SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|...    27   1.6  
SPBC146.11c |mug97|meu33|meiotically upregulated gene Mug97|Schi...    27   2.2  
SPCC320.08 |||membrane transporter |Schizosaccharomyces pombe|ch...    27   2.2  
SPAC31G5.10 |eta2||Myb family transcriptional regulator Eta2|Sch...    27   2.2  
SPCC16A11.03c |||DUF2009 protein|Schizosaccharomyces pombe|chr 3...    26   3.8  
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb...    25   6.7  
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    25   6.7  
SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pom...    25   8.8  
SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr...    25   8.8  

>SPBC2F12.15c |||palmitoyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 329

 Score = 52.8 bits (121), Expect = 3e-08
 Identities = 30/79 (37%), Positives = 42/79 (53%), Gaps = 1/79 (1%)
 Frame = +1

Query: 1   LKMDHHCPWVNNCVCFHNYKFFMLFLGYALLYCLFVMATCLPYFIRLWKGDFGTATSSG- 177
           L+MDHHC W  NCV F N+KFF L   Y  LY + V+ +    F+ + K    T T+ G 
Sbjct: 121 LRMDHHCMWFKNCVGFRNHKFFFLECFYLNLYSICVLYST---FVAITK----TFTAEGA 173

Query: 178 RYHIIFAFFVSLMFAISLG 234
               I+  F   +FA ++G
Sbjct: 174 NISAIYLVFWGFLFAFAVG 192



 Score = 52.8 bits (121), Expect = 3e-08
 Identities = 22/61 (36%), Positives = 39/61 (63%)
 Frame = +3

Query: 258 LVVNNRTTLEAFRAPMFRGGADKNGFSIGAFKNFKEVFGNSPNLWLVPVFTSLGDGCEYP 437
           L+++N +TLE+  +   R       F++G ++N+ ++ G SP LWL+P   S+G+G EYP
Sbjct: 207 LLIHNLSTLESMSSSWSRYTHSTQPFNVGWYENWCQIMGKSPFLWLLPFPNSIGEGVEYP 266

Query: 438 V 440
           +
Sbjct: 267 L 267


>SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 350

 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 31/111 (27%), Positives = 56/111 (50%), Gaps = 9/111 (8%)
 Frame = +1

Query: 7   MDHHCPWVNNCVCFHNYKFFMLFLGYALLYCLFVMATCLPYFIRLWKGDFGTATSSG--- 177
           +DHHC W+N C+   NY+++ +FL   +L  L++  T L ++  +  G F  +T +    
Sbjct: 208 LDHHCIWLNTCIGRRNYRYYFIFLLSVVLSALYL--TGLGFYTSI--GSFHESTDTNFAA 263

Query: 178 ---RYHIIFAFFVSL---MFAISLGSLFGYHCTSSSIIEQL*KRLERRCSE 312
              R     +FF+ +   + AI  G LF Y C   S+ + + + L  + +E
Sbjct: 264 HLRRPWAGVSFFLGIYGALGAILPGILFCYQCYLISVGQNVHEYLRAKSTE 314


>SPBC13G1.07 |||palmitoyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 356

 Score = 44.0 bits (99), Expect = 1e-05
 Identities = 15/25 (60%), Positives = 19/25 (76%)
 Frame = +1

Query: 4   KMDHHCPWVNNCVCFHNYKFFMLFL 78
           K DHHC W+NNCV  +N ++F LFL
Sbjct: 183 KFDHHCIWINNCVGLNNARYFFLFL 207


>SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 642

 Score = 40.7 bits (91), Expect = 1e-04
 Identities = 13/25 (52%), Positives = 18/25 (72%)
 Frame = +1

Query: 4   KMDHHCPWVNNCVCFHNYKFFMLFL 78
           + DHHCPW  NCV   N++ F+LF+
Sbjct: 425 RYDHHCPWTGNCVGARNHRTFLLFV 449


>SPBC691.01 |||palmitoyltransferase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 312

 Score = 39.5 bits (88), Expect = 3e-04
 Identities = 17/41 (41%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
 Frame = +1

Query: 4   KMDHHCPWVNNCVCFHNYKFFMLFLGYAL-LYCLFVMATCL 123
           K DH+C +V   VCF N KFF  FL Y     C+ +++T +
Sbjct: 138 KFDHYCSFVGKDVCFSNQKFFYQFLFYGFSAACMVLISTAI 178


>SPAC4D7.12c |fet5||transcription factor Fet5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 276

 Score = 28.7 bits (61), Expect = 0.54
 Identities = 10/17 (58%), Positives = 12/17 (70%)
 Frame = -2

Query: 275 SIIDDEVQWYPNSEPRD 225
           S IDD  QWY + EP+D
Sbjct: 247 SYIDDATQWYEDQEPKD 263


>SPCC306.06c |||ER membrane protein, BIG1 family
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 311

 Score = 28.3 bits (60), Expect = 0.71
 Identities = 13/29 (44%), Positives = 17/29 (58%)
 Frame = +3

Query: 315 GADKNGFSIGAFKNFKEVFGNSPNLWLVP 401
           G + N F +GAFK+ K V   SP   L+P
Sbjct: 71  GLEVNDFQLGAFKHLKRVSLQSPYSLLLP 99


>SPBC2D10.04 |||arrestin Aly1 related|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 658

 Score = 28.3 bits (60), Expect = 0.71
 Identities = 19/67 (28%), Positives = 32/67 (47%)
 Frame = -3

Query: 406 NTGTNQRFGLLPNTSLKFLKAPIEKPFLSAPPRNIGALNASKVVLLLTTRYNGIRIVNPE 227
           N+ +N    +LPN +L  ++    KP L+  P +      SKV  ++    N   +V P 
Sbjct: 77  NSSSNTGGNVLPNPALTPVRNMSNKPPLTWSPSSANLFGTSKVTSIIGN--NVSDVVPPV 134

Query: 226 ISRTLAT 206
           I + LA+
Sbjct: 135 IKKALAS 141


>SPBC409.20c |psh3||ER chaperone SHR3 homologue
           Psh3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 215

 Score = 27.9 bits (59), Expect = 0.94
 Identities = 15/51 (29%), Positives = 24/51 (47%)
 Frame = +1

Query: 184 HIIFAFFVSLMFAISLGSLFGYHCTSSSIIEQL*KRLERRCSEAAPTKMAS 336
           H++  F ++ +  I LG +FGYH  S    E      E+  +E A  +  S
Sbjct: 147 HVLVEFLLAGVILIQLGYVFGYHVQSIQQREYAEDMREQELAEKAKLESES 197


>SPBP8B7.26 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 262

 Score = 27.9 bits (59), Expect = 0.94
 Identities = 12/39 (30%), Positives = 21/39 (53%)
 Frame = -3

Query: 424 PSPRLVNTGTNQRFGLLPNTSLKFLKAPIEKPFLSAPPR 308
           P PR V+  ++   G +P  +     +P++KP   APP+
Sbjct: 191 PPPRRVSQNSSYASGSVPAATAASTASPVKKPPPPAPPK 229


>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 3699

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 13/35 (37%), Positives = 18/35 (51%)
 Frame = -3

Query: 376  LPNTSLKFLKAPIEKPFLSAPPRNIGALNASKVVL 272
            L + SL+F   P+ KP LS+ P      N S + L
Sbjct: 2406 LTDNSLRFAPVPMTKPILSSLPEVFSKHNGSAIPL 2440


>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1628

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 9/14 (64%), Positives = 12/14 (85%)
 Frame = +3

Query: 69  AVPWLRAAVLSLCH 110
           A+PWLR + L+LCH
Sbjct: 539 AIPWLRLSGLALCH 552


>SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 530

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 30/105 (28%), Positives = 45/105 (42%)
 Frame = +1

Query: 163 ATSSGRYHIIFAFFVSLMFAISLGSLFGYHCTSSSIIEQL*KRLERRCSEAAPTKMASQL 342
           ATS+  Y    +  V+    IS GS   Y  TSSS I      +    S +A  K  S  
Sbjct: 190 ATSTSSYVAPVSSSVASSSEISAGSATSYVPTSSSSIAL--SSVVASASVSAANKGVSTP 247

Query: 343 VLSKTSRKCSVIVRTSGWCLYSQASATAANIRYVANTSCRRSRAP 477
            +S  +   S +V +    + S A++ AA+    + TS   S +P
Sbjct: 248 AVSSAAASSSAVVSS----VVSSATSVAASSTISSATSSSASASP 288


>SPBC146.11c |mug97|meu33|meiotically upregulated gene
           Mug97|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score = 26.6 bits (56), Expect = 2.2
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = +3

Query: 375 NSPNLWLVPVFTSLGDGCEYPVRREH 452
           N P+++ V    S G  CEY +RR+H
Sbjct: 204 NRPSIFYVLQGISFGPECEYVLRRKH 229


>SPCC320.08 |||membrane transporter |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 505

 Score = 26.6 bits (56), Expect = 2.2
 Identities = 21/65 (32%), Positives = 33/65 (50%), Gaps = 3/65 (4%)
 Frame = +1

Query: 67  MLFLGYALLYCLFVMATCLPYFIRLWKGDFGTATSSGR---YHIIFAFFVSLMFAISLGS 237
           ++FL Y+L   LF M++   Y I L   DF +         YH+ + + ++ +  I LG 
Sbjct: 370 VMFLLYSLAPILFRMSSATFYNISLLTSDFWSLVIGIHVFGYHVYWLYPIAFVLII-LG- 427

Query: 238 LFGYH 252
           LF YH
Sbjct: 428 LFVYH 432


>SPAC31G5.10 |eta2||Myb family transcriptional regulator
           Eta2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 569

 Score = 26.6 bits (56), Expect = 2.2
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = +2

Query: 392 VGACIHKPRRRLRISGTSRTPAADVHE 472
           +G+ IHKP R   + GTS  P +D H+
Sbjct: 79  MGSLIHKPSRAHSLGGTSE-PVSDDHK 104


>SPCC16A11.03c |||DUF2009 protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 466

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 11/36 (30%), Positives = 19/36 (52%)
 Frame = +3

Query: 15  PLPLGEQLRLLPQLQVLHAVPWLRAAVLSLCHGDMP 122
           PL      R+L  +Q    +PW+ ++V+ L   D+P
Sbjct: 325 PLVRSTVTRILSSVQKQQEIPWMGSSVIHLGDRDVP 360


>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1207

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
 Frame = -3

Query: 316 PPRNIGALNASKVVLLLTTRYNGI-RIVNPEISRTLATRKKRI*CDNDHSMSLYRSR 149
           PPR++    A KV LL ++  NG+  +VN          K  I    D ++ + R+R
Sbjct: 374 PPRSLSYNPAEKVALLTSSADNGVYELVNVSSRSNSLPLKDNIKGPGDDAIFVARNR 430


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 25/78 (32%), Positives = 35/78 (44%), Gaps = 2/78 (2%)
 Frame = +3

Query: 168 IEWSLSHH--IRFFRVANVRDISGFTIRIPLYLVVNNRTTLEAFRAPMFRGGADKNGFSI 341
           IE S+S H  +R  + A++  +S    +  L+LV  N   L  F       G D      
Sbjct: 690 IEDSISLHRDVRDSKDAHILKVSNKLRKQILHLVEQN--CLLIFD---LHNGTDDTIVKS 744

Query: 342 GAFKNFKEVFGNSPNLWL 395
             F+ F+E FGNS   WL
Sbjct: 745 HNFQKFEEEFGNSRKEWL 762


>SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1188

 Score = 24.6 bits (51), Expect = 8.8
 Identities = 11/26 (42%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
 Frame = -3

Query: 376 LPNTSLKFLKAPIEKPFLSA-PPRNI 302
           +PN S++    P EKP+LS+  P N+
Sbjct: 85  VPNQSIQGSSLPSEKPYLSSNQPTNV 110


>SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1060

 Score = 24.6 bits (51), Expect = 8.8
 Identities = 14/43 (32%), Positives = 23/43 (53%)
 Frame = -3

Query: 331 PFLSAPPRNIGALNASKVVLLLTTRYNGIRIVNPEISRTLATR 203
           P L   P +   +++SK    L T  NG+R   PE++  ++TR
Sbjct: 517 PRLRGSPIHNQPVSSSKSTASLNTNNNGLRASTPEMA-NISTR 558


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,264,178
Number of Sequences: 5004
Number of extensions: 49470
Number of successful extensions: 159
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 206265012
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -