BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00240 (838 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g17220.1 68418.m02018 glutathione S-transferase, putative 39 0.005 At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 36 0.025 At2g30870.1 68415.m03762 glutathione S-transferase, putative sup... 36 0.044 At3g03190.1 68416.m00315 glutathione S-transferase, putative ide... 35 0.058 At1g75270.1 68414.m08744 dehydroascorbate reductase, putative si... 33 0.31 At4g02520.1 68417.m00345 glutathione S-transferase, putative 32 0.41 At2g02930.1 68415.m00241 glutathione S-transferase, putative 32 0.41 At1g49860.1 68414.m05590 glutathione S-transferase, putative sim... 32 0.41 At5g67385.1 68418.m08497 phototropic-responsive protein, putativ... 31 0.72 At4g22300.1 68417.m03225 phospholipase/carboxylesterase family p... 31 0.72 At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 31 0.72 At3g12970.1 68416.m01616 expressed protein 31 0.95 At5g41220.1 68418.m05009 glutathione S-transferase, putative sim... 31 1.3 At3g62760.1 68416.m07050 glutathione S-transferase, putative Glu... 31 1.3 At3g63400.1 68416.m07137 peptidyl-prolyl cis-trans isomerase cyc... 30 2.2 At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) iden... 30 2.2 At1g56020.1 68414.m06431 expressed protein 30 2.2 At1g06160.1 68414.m00647 ethylene-responsive factor, putative si... 29 3.8 At1g29880.1 68414.m03652 glycyl-tRNA synthetase / glycine--tRNA ... 29 5.1 At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 29 5.1 At5g41240.1 68418.m05011 glutathione S-transferase, putative sim... 28 6.7 At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiqui... 28 6.7 At1g68930.1 68414.m07889 pentatricopeptide (PPR) repeat-containi... 28 6.7 At1g19570.1 68414.m02437 dehydroascorbate reductase, putative si... 28 6.7 At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1... 28 8.8 At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1... 28 8.8 At1g06760.1 68414.m00718 histone H1, putative similar to histone... 28 8.8 >At5g17220.1 68418.m02018 glutathione S-transferase, putative Length = 214 Score = 38.7 bits (86), Expect = 0.005 Identities = 25/70 (35%), Positives = 36/70 (51%), Gaps = 6/70 (8%) Frame = +2 Query: 50 QRLLLAY--GGQEFEDNRISSENWPEFKP----KTPFGQMPVLEIDGKQYAQSTAICRYL 211 QR+LL + G EFE I + + + KP + PFGQ+P +E + +S AI RY Sbjct: 15 QRVLLCFLEKGIEFEIIHIDLDTFEQKKPEHLLRQPFGQVPAIEDGDFKLFESRAIARYY 74 Query: 212 GRKYGLAGAN 241 K+ G N Sbjct: 75 ATKFADQGTN 84 >At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / eEF-1B gamma, putative similar to elongation factor 1B gamma GI:3868758 from [Oryza sativa] Length = 413 Score = 36.3 bits (80), Expect = 0.025 Identities = 17/37 (45%), Positives = 21/37 (56%) Frame = +2 Query: 116 PEFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYG 226 PEF P G++PVLE +S AI RY+ RK G Sbjct: 43 PEFLKMNPIGKVPVLETPEGPIFESNAIARYVSRKNG 79 >At2g30870.1 68415.m03762 glutathione S-transferase, putative supported by cDNA GI:443698 GB:D17673 Length = 215 Score = 35.5 bits (78), Expect = 0.044 Identities = 25/77 (32%), Positives = 37/77 (48%), Gaps = 5/77 (6%) Frame = +2 Query: 20 YFPVKALGESQRLLLAYGGQEFEDNRIS----SENWPEFKPKTPFGQMPVLEIDGK-QYA 184 Y P+ A + + L G FE + + PE+ PFG++PVL +DG + Sbjct: 6 YAPLFASSKRAVVTLVEKGVSFETVNVDLMKGEQRQPEYLAIQPFGKIPVL-VDGDYKIF 64 Query: 185 QSTAICRYLGRKYGLAG 235 +S AI RY+ KY G Sbjct: 65 ESRAIMRYIAEKYRSQG 81 >At3g03190.1 68416.m00315 glutathione S-transferase, putative identical to glutathione S-transferase GB:AAB09584 from [Arabidopsis thaliana] Length = 214 Score = 35.1 bits (77), Expect = 0.058 Identities = 26/80 (32%), Positives = 39/80 (48%), Gaps = 6/80 (7%) Frame = +2 Query: 20 YFPVKALGESQRLLLAYGGQ--EFEDNRISSENWPEFKP----KTPFGQMPVLEIDGKQY 181 Y +KA QR+LL + + EFE + + + KP + PFGQ+P +E + Sbjct: 6 YGQIKA-ANPQRVLLCFLEKDIEFEVIHVDLDKLEQKKPQHLLRQPFGQVPAIEDGYLKL 64 Query: 182 AQSTAICRYLGRKYGLAGAN 241 +S AI RY KY G + Sbjct: 65 FESRAIARYYATKYADQGTD 84 >At1g75270.1 68414.m08744 dehydroascorbate reductase, putative similar to GI:6939839 from [Oryza sativa] Length = 213 Score = 32.7 bits (71), Expect = 0.31 Identities = 21/62 (33%), Positives = 36/62 (58%), Gaps = 3/62 (4%) Frame = +2 Query: 47 SQRLLLAYGGQE--FEDNRISSENWPE-FKPKTPFGQMPVLEIDGKQYAQSTAICRYLGR 217 SQR+LL ++ ++ + I+ + P+ F +P G++PV+++DGK A S I L Sbjct: 23 SQRVLLTLEEKKLPYKTHLINVSDKPQWFLDISPEGKVPVVKLDGKWVADSDVIVGLLEE 82 Query: 218 KY 223 KY Sbjct: 83 KY 84 >At4g02520.1 68417.m00345 glutathione S-transferase, putative Length = 212 Score = 32.3 bits (70), Expect = 0.41 Identities = 14/40 (35%), Positives = 21/40 (52%) Frame = +2 Query: 122 FKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYGLAGAN 241 F + PFGQ+P E + +S AI +Y+ +Y G N Sbjct: 46 FLSRNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTN 85 >At2g02930.1 68415.m00241 glutathione S-transferase, putative Length = 212 Score = 32.3 bits (70), Expect = 0.41 Identities = 14/40 (35%), Positives = 21/40 (52%) Frame = +2 Query: 122 FKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYGLAGAN 241 F + PFGQ+P E + +S AI +Y+ +Y G N Sbjct: 46 FLSRNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTN 85 >At1g49860.1 68414.m05590 glutathione S-transferase, putative similar to GI:860955 from [Hyoscyamus muticus] (Plant Physiol. 109 (1), 253-260 (1995)) Length = 254 Score = 32.3 bits (70), Expect = 0.41 Identities = 15/35 (42%), Positives = 20/35 (57%) Frame = +2 Query: 137 PFGQMPVLEIDGKQYAQSTAICRYLGRKYGLAGAN 241 PFG++PVLE + + AI RYL +Y G N Sbjct: 53 PFGEVPVLEDGDLKLFEPKAITRYLAEQYKDVGTN 87 >At5g67385.1 68418.m08497 phototropic-responsive protein, putative similar to root phototropism RPT2 [Arabidopsis thaliana] gi|6959488|gb|AAF33112, a signal transducer of phototropic response PMID:10662859 Length = 663 Score = 31.5 bits (68), Expect = 0.72 Identities = 16/41 (39%), Positives = 24/41 (58%) Frame = +3 Query: 129 LRLRSVRCRCWKSTASSTLRAPPSAGTSVASTGSPGPMTRR 251 ++L+ K T+SST+ PS+ S ASTG P P+ R+ Sbjct: 586 MKLKEFEKESEKKTSSSTISTNPSSPISTASTGKP-PLPRK 625 >At4g22300.1 68417.m03225 phospholipase/carboxylesterase family protein similar to acyl-protein thioesterase-1 [Homo sapiens] GI:9965372; contains Pfam profile PF02230: Phospholipase/Carboxylesterase family Length = 471 Score = 31.5 bits (68), Expect = 0.72 Identities = 13/31 (41%), Positives = 18/31 (58%), Gaps = 1/31 (3%) Frame = -1 Query: 742 ERRNISWFHPDSPPNPYSPRF-TVLPVFFTL 653 E RN W P +PPNP S + V+P +F + Sbjct: 315 EFRNTKWLFPSAPPNPVSCNYGAVMPSWFDI 345 >At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / eEF-1B gamma, putative Similar to elongation factor 1-gamma (gb|EF1G_XENLA). ESTs gb|T20564,gb|T45940,gb|T04527 come from this gene Length = 414 Score = 31.5 bits (68), Expect = 0.72 Identities = 15/37 (40%), Positives = 19/37 (51%) Frame = +2 Query: 116 PEFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYG 226 P F P G++PVLE +S AI RY+ R G Sbjct: 43 PAFLKMNPIGKVPVLETPEGSVFESNAIARYVSRLNG 79 >At3g12970.1 68416.m01616 expressed protein Length = 381 Score = 31.1 bits (67), Expect = 0.95 Identities = 14/33 (42%), Positives = 18/33 (54%) Frame = +3 Query: 219 STGSPGPMTRRPRDRPERGVPERHSRQCRIGPL 317 S+ SPG T PR + G+P HS RI P+ Sbjct: 289 SSSSPGNFTGGPRMKLHHGMPRSHSANVRITPV 321 >At5g41220.1 68418.m05009 glutathione S-transferase, putative similar to emb|CAA10662 Length = 590 Score = 30.7 bits (66), Expect = 1.3 Identities = 16/37 (43%), Positives = 23/37 (62%), Gaps = 1/37 (2%) Frame = +2 Query: 116 PEFKPKTPFGQMPVLEIDGK-QYAQSTAICRYLGRKY 223 PEFK P G++P + +DGK + ++S AI YL Y Sbjct: 43 PEFKDINPMGKVPAI-VDGKLKLSESHAILIYLSSAY 78 >At3g62760.1 68416.m07050 glutathione S-transferase, putative Glutathione transferase III(b) - Zea mays, EMBL:AJ010296 Length = 219 Score = 30.7 bits (66), Expect = 1.3 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = +2 Query: 116 PEFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKY 223 P F PFG++P L+ D +S AI Y+ K+ Sbjct: 43 PSFLSMNPFGKVPALQDDDLTLFESRAITAYIAEKH 78 >At3g63400.1 68416.m07137 peptidyl-prolyl cis-trans isomerase cyclophilin-type family protein similar to cyclophylin [Digitalis lanata] GI:1563719; contains Pfam profile PF00160: peptidyl-prolyl cis-trans isomerase, cyclophilin-type; contains AT-donor splice site at intron 9 Length = 570 Score = 29.9 bits (64), Expect = 2.2 Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 4/42 (9%) Frame = -3 Query: 251 PPRHWPRRARTCDRGTCRW----RCSERTACRRFPAPASDRT 138 PPRHWP R DR R+ SER+ RF +P R+ Sbjct: 438 PPRHWPDRRNFQDRNRDRYPSNRSYSERSPRGRFRSPPRRRS 479 >At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) identical to GB:X95295. Based on identical cDNA hits, the translation is now 40 AAs longer at the N-terminal, and start of exon2 is also corrected. Length = 263 Score = 29.9 bits (64), Expect = 2.2 Identities = 14/33 (42%), Positives = 18/33 (54%) Frame = +2 Query: 137 PFGQMPVLEIDGKQYAQSTAICRYLGRKYGLAG 235 PFGQ+P LE +S AI +YL +Y G Sbjct: 99 PFGQIPALEDGDLTLFESRAITQYLAEEYSEKG 131 >At1g56020.1 68414.m06431 expressed protein Length = 398 Score = 29.9 bits (64), Expect = 2.2 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = +3 Query: 219 STGSPGPMTRRPRDRPERGVPERHSRQCRIGPL 317 S+ SPG T PR + G+P +S RI P+ Sbjct: 306 SSSSPGSFTGGPRMKQHHGMPRSYSANVRITPV 338 >At1g06160.1 68414.m00647 ethylene-responsive factor, putative similar to ethylene response factor 1 GB:AAD03544 GI:4128208 from [Arabidopsis thaliana] Length = 244 Score = 29.1 bits (62), Expect = 3.8 Identities = 13/32 (40%), Positives = 22/32 (68%) Frame = +3 Query: 162 KSTASSTLRAPPSAGTSVASTGSPGPMTRRPR 257 KS++SSTL + PS+ +S +S+ S ++ R R Sbjct: 180 KSSSSSTLTSSPSSSSSYSSSSSSSSLSSRSR 211 >At1g29880.1 68414.m03652 glycyl-tRNA synthetase / glycine--tRNA ligase identical to SP|O23627 Glycyl-tRNA synthetase (EC 6.1.1.14) (Glycine--tRNA ligase) (GlyRS) {Arabidopsis thaliana} Length = 729 Score = 28.7 bits (61), Expect = 5.1 Identities = 11/17 (64%), Positives = 12/17 (70%) Frame = -1 Query: 826 QSCFVNFQTFYYYNPKK 776 Q FVNF+ YYYN KK Sbjct: 299 QGIFVNFKDLYYYNGKK 315 >At1g02940.1 68414.m00261 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus] Length = 256 Score = 28.7 bits (61), Expect = 5.1 Identities = 13/32 (40%), Positives = 17/32 (53%) Frame = +2 Query: 116 PEFKPKTPFGQMPVLEIDGKQYAQSTAICRYL 211 P F PFGQ+PV G + +S AI Y+ Sbjct: 79 PSFLAINPFGQVPVFLDGGLKLTESRAISEYI 110 >At5g41240.1 68418.m05011 glutathione S-transferase, putative similar to glutathione S-transferase, GST 10b GB:CAA10662 [Arabidopsis thaliana] 37349. Length = 591 Score = 28.3 bits (60), Expect = 6.7 Identities = 15/37 (40%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = +2 Query: 116 PEFKPKTPFGQMPVLEIDGK-QYAQSTAICRYLGRKY 223 PEFK P G++P + +DG+ + +S AI YL Y Sbjct: 43 PEFKEINPMGKVPAI-VDGRLKLFESHAILIYLSSAY 78 >At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiquitin-transferase family protein similar to SP|Q14669Thyroid receptor interacting protein 12 (TRIP12) {Homo sapiens}; contains Pfam profile PF00632: HECT-domain (ubiquitin-transferase) Length = 1888 Score = 28.3 bits (60), Expect = 6.7 Identities = 15/41 (36%), Positives = 19/41 (46%) Frame = +3 Query: 138 RSVRCRCWKSTASSTLRAPPSAGTSVASTGSPGPMTRRPRD 260 RS R R S++SS PPS+ T+ S S P D Sbjct: 35 RSKRARLSSSSSSSLAPTPPSSSTTTRSRSSRSAAAAAPMD 75 >At1g68930.1 68414.m07889 pentatricopeptide (PPR) repeat-containing protein contains INTERPRO:IPR002885 PPR repeats Length = 743 Score = 28.3 bits (60), Expect = 6.7 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +1 Query: 406 LTKNNGHIALGKLTWGDFVYAGMYDYL 486 L+ +NGH++LGK G + G YL Sbjct: 148 LSSSNGHVSLGKQIHGQVIKLGFESYL 174 >At1g19570.1 68414.m02437 dehydroascorbate reductase, putative similar to GB:BAA90672 from (Oryza sativa) Length = 213 Score = 28.3 bits (60), Expect = 6.7 Identities = 21/62 (33%), Positives = 32/62 (51%), Gaps = 3/62 (4%) Frame = +2 Query: 47 SQRLLLAYGGQE--FEDNRISSENWPE-FKPKTPFGQMPVLEIDGKQYAQSTAICRYLGR 217 SQR LL + ++ + I+ + P+ F +P G++PVL+ID K S I L Sbjct: 23 SQRALLTLEEKSLTYKIHLINLSDKPQWFLDISPQGKVPVLKIDDKWVTDSDVIVGILEE 82 Query: 218 KY 223 KY Sbjct: 83 KY 84 >At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 191 Score = 27.9 bits (59), Expect = 8.8 Identities = 23/77 (29%), Positives = 35/77 (45%), Gaps = 5/77 (6%) Frame = +2 Query: 8 VKFYYFPVKALGESQRLLLAYGGQEFED---NRISSENWP-EFKPKTPFGQMPVLEIDGK 175 +K Y + + R+ LA G ++E N + + + +FK P G +P L +DG Sbjct: 9 LKLYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDSDFKKINPMGTVPAL-VDGD 67 Query: 176 QYA-QSTAICRYLGRKY 223 S AI YL KY Sbjct: 68 VVINDSFAIIMYLDEKY 84 >At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 221 Score = 27.9 bits (59), Expect = 8.8 Identities = 23/77 (29%), Positives = 35/77 (45%), Gaps = 5/77 (6%) Frame = +2 Query: 8 VKFYYFPVKALGESQRLLLAYGGQEFED---NRISSENWP-EFKPKTPFGQMPVLEIDGK 175 +K Y + + R+ LA G ++E N + + + +FK P G +P L +DG Sbjct: 9 LKLYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDSDFKKINPMGTVPAL-VDGD 67 Query: 176 QYA-QSTAICRYLGRKY 223 S AI YL KY Sbjct: 68 VVINDSFAIIMYLDEKY 84 >At1g06760.1 68414.m00718 histone H1, putative similar to histone H1-1 GB:CAA44312 GI:16314 from [Arabidopsis thaliana]; identical to cDNA H1-1C mRNA for histone H1-1 (partial) GI:732560 Length = 274 Score = 27.9 bits (59), Expect = 8.8 Identities = 17/47 (36%), Positives = 29/47 (61%) Frame = +2 Query: 488 SDAPEAGLEQKYPAFRKPIEAVLAIPKVKAYVDAAPRTEL*KSISVQ 628 + +P+A E+ PA +KP A +A+ K K V AA + + K+I+V+ Sbjct: 135 ASSPKAAAEKSAPAKKKP--ATVAVTKAKRKVAAASKAK--KTIAVK 177 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,432,622 Number of Sequences: 28952 Number of extensions: 292841 Number of successful extensions: 936 Number of sequences better than 10.0: 27 Number of HSP's better than 10.0 without gapping: 901 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 936 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1931371200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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