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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00235
         (816 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC970.06 |||cargo receptor for soluble proteins |Schizosacchar...    40   4e-04
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|...    30   0.45 
SPCC16C4.01 |sif2|SPCC5E4.09|Sad1 interacting factor 2|Schizosac...    29   0.60 
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    29   1.0  
SPAC29A4.06c |||human CCDC55 homolog|Schizosaccharomyces pombe|c...    27   3.2  
SPCC576.15c |ksg1||serine/threonine protein kinase Ksg1|Schizosa...    26   5.6  
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    26   7.4  
SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|...    26   7.4  
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc...    26   7.4  
SPBC32H8.11 |mei4||meiotic forkhead transcription factor Mei4 |S...    26   7.4  
SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor Raf2|S...    25   9.7  
SPBC16H5.11c |skb1|rmt5|type II protein arginine N-methyltransfe...    25   9.7  
SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo...    25   9.7  
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    25   9.7  
SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi...    25   9.7  

>SPCC970.06 |||cargo receptor for soluble proteins
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 302

 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 37/162 (22%), Positives = 62/162 (38%), Gaps = 5/162 (3%)
 Frame = +2

Query: 284 MFVIVNLFGQLGGCVMVLGRLKVDIACGVLFFIVVLQTFAYSILW--DVQFXXXXXXXXX 457
           +FV V L   L G  +V+ + +   A G L F+ +LQ FAY ++   ++ F         
Sbjct: 99  LFVCVVLM--LVGSTLVVFKKRQAYAIGSLLFVTLLQAFAYGLITSGEMFFRNMSVIGGL 156

Query: 458 XXXXXXXXXXXXXXXCSGRAVARREQTEDVLATRRRILLAFMFITLLRFE---ISFLQII 628
                           +G                 R+LL FMF+ LL  E   IS+ +I+
Sbjct: 157 CLVASDTFIHRRINRFAGLPAVSEHNKRTYFQLAGRVLLIFMFLGLLAKEGSGISWTRIL 216

Query: 629 QDLLGSILMILVTVGYRTKXXXXXXXXXXXXXXXYHNAWWAV 754
             +L      +V +G++ K                 N++W+V
Sbjct: 217 VHILSVTACAMVVIGFKAKFFAAVLVLILSVANFIINSFWSV 258



 Score = 34.3 bits (75), Expect = 0.021
 Identities = 12/31 (38%), Positives = 20/31 (64%)
 Frame = +3

Query: 156 LPTVARLCLISTFLEDGLRMWFQWSEQRDYM 248
           +P + R  +++T+ ED +R+  QW EQ  YM
Sbjct: 54  MPLLGRFLIVATYFEDAIRIVTQWPEQVSYM 84



 Score = 33.5 bits (73), Expect = 0.037
 Identities = 12/17 (70%), Positives = 15/17 (88%)
 Frame = +3

Query: 765 PLRDFLKYDFFQTLSVI 815
           P RDF +YDFFQTLS++
Sbjct: 263 PYRDFYRYDFFQTLSIV 279


>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
           subunit|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1752

 Score = 29.9 bits (64), Expect = 0.45
 Identities = 10/23 (43%), Positives = 14/23 (60%)
 Frame = +3

Query: 189 TFLEDGLRMWFQWSEQRDYMDMP 257
           T  +DGLR+W  W   +D  D+P
Sbjct: 181 TIRKDGLRLWGSWKRGKDESDLP 203


>SPCC16C4.01 |sif2|SPCC5E4.09|Sad1 interacting factor
           2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 446

 Score = 29.5 bits (63), Expect = 0.60
 Identities = 17/47 (36%), Positives = 24/47 (51%)
 Frame = +3

Query: 84  QIPNEYVSTAEDVADQVIRKGKNVLPTVARLCLISTFLEDGLRMWFQ 224
           QIP    STA+  A+ + ++ +  LP V   C   TF  D L  +FQ
Sbjct: 41  QIPQIEGSTAKRDAEHLGKRHREFLPRVTAYCTCDTFRVDLLFKFFQ 87


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 28.7 bits (61), Expect = 1.0
 Identities = 18/54 (33%), Positives = 26/54 (48%)
 Frame = -1

Query: 378 KNSTPQAISTLSLPSTITHPPN*PNKFTITNIVARNLPQPQACPCSLSALTTGT 217
           KN  PQ +  +   ++    PN P  F++  + +   PQP   P S S  TTGT
Sbjct: 19  KNGVPQEVIVIEDSASPRLTPNLPPPFSVHQLQSFVPPQP---PSSSSPSTTGT 69


>SPAC29A4.06c |||human CCDC55 homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 355

 Score = 27.1 bits (57), Expect = 3.2
 Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 5/56 (8%)
 Frame = -3

Query: 721 NCQYEDK---HQSRQLRPVAHRHQYHQDAPEKI--LYDLQE*DFKS*ERDEHEGQQ 569
           N   +DK   H+S   R ++   +YHQD P+K    Y L+E D    +R E E +Q
Sbjct: 256 NSMRDDKKRNHKSSYKRSLSPSTRYHQDRPDKRHGTYSLEEIDK---QRKEFENRQ 308


>SPCC576.15c |ksg1||serine/threonine protein kinase
           Ksg1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 592

 Score = 26.2 bits (55), Expect = 5.6
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = -1

Query: 414 SILYANVCNTTMKNSTPQAISTLSLPSTITH 322
           +I  +N  NTT   ST Q  S+  +PS + H
Sbjct: 48  AIPQSNALNTTPNESTSQIDSSPKIPSAVPH 78


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 25.8 bits (54), Expect = 7.4
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = -2

Query: 494  PQLGPPPTPAVALRSMPNCGE 432
            P   PPP PA +  S+PN G+
Sbjct: 1728 PSAPPPPLPASSAPSVPNPGD 1748


>SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 743

 Score = 25.8 bits (54), Expect = 7.4
 Identities = 13/25 (52%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
 Frame = -1

Query: 387 TTMKNSTPQAISTLSLPST-ITHPP 316
           T    STPQ  +T S P+T  THPP
Sbjct: 279 TPQSGSTPQMQNTTSQPTTHDTHPP 303


>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1016

 Score = 25.8 bits (54), Expect = 7.4
 Identities = 28/106 (26%), Positives = 37/106 (34%), Gaps = 2/106 (1%)
 Frame = +1

Query: 163  LW-RDCALYP-HSWRTVSACGSSGQSRETTWTCLGLRQIPRHNVCDSKFIWSIRRMCYGA 336
            LW  D   YP  S  T +   S+G    T+ T      +P  +   +    SI   C GA
Sbjct: 737  LWANDAVYYPVSSSSTTTTSTSTGSVTTTSTTATASCTLPI-SYTSTPTTTSISGTCNGA 795

Query: 337  RKAQSGYRLRRAVLHSCITDIRVQYTMGRTVPSPQFGIDRSATAGV 474
                S Y     VL   +  +  Q   G      Q+G D  A   V
Sbjct: 796  TFDASLYVCDGTVLCPIVNGVSYQNCNGACYNPSQYGCDNGALGPV 841


>SPBC32H8.11 |mei4||meiotic forkhead transcription factor Mei4
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 517

 Score = 25.8 bits (54), Expect = 7.4
 Identities = 16/57 (28%), Positives = 26/57 (45%)
 Frame = -1

Query: 423 TSHSILYANVCNTTMKNSTPQAISTLSLPSTITHPPN*PNKFTITNIVARNLPQPQA 253
           TS S   +NV      +++  +    SL S I  PP  P+     +  + N+P+P A
Sbjct: 215 TSTSGSSSNVAAEVSNDASQPSNQDSSLNSNIVKPPLPPSNVQSNSSSSENVPKPNA 271


>SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor
           Raf2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 636

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 10/18 (55%), Positives = 15/18 (83%)
 Frame = -1

Query: 390 NTTMKNSTPQAISTLSLP 337
           NTTMK+S  QA++ ++LP
Sbjct: 226 NTTMKSSLNQALTLINLP 243


>SPBC16H5.11c |skb1|rmt5|type II protein arginine
           N-methyltransferase Skb1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 645

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 7/11 (63%), Positives = 10/11 (90%)
 Frame = +3

Query: 195 LEDGLRMWFQW 227
           L DG+R+WF+W
Sbjct: 601 LTDGMRVWFEW 611


>SPBC19G7.10c |||topoisomerase associated protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 744

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 8/26 (30%), Positives = 15/26 (57%)
 Frame = -3

Query: 808 DNVWKKSYLRKSLSGCRMHRPPRVMV 731
           +++WK  Y+ +SL      RPP + +
Sbjct: 500 ESIWKALYINESLEASSKTRPPFISI 525


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 23/74 (31%), Positives = 34/74 (45%), Gaps = 3/74 (4%)
 Frame = -1

Query: 429 NCT-SHSILYANVCNTTMKNSTPQAISTLSLPSTITHPPN*PNKFTITNI--VARNLPQP 259
           NCT S S+LY +   T+   +T    ++ S+P T T  P   + +TI++   V       
Sbjct: 531 NCTTSTSVLYTSTPVTSTPLATTNCTTSTSVPYTST--PVTSSNYTISSSTPVTSTPVTT 588

Query: 258 QACPCSLSALTTGT 217
             C  S S L T T
Sbjct: 589 TNCTTSTSVLYTST 602


>SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA
           reductase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1053

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 11/40 (27%), Positives = 20/40 (50%)
 Frame = -1

Query: 435 RRNCTSHSILYANVCNTTMKNSTPQAISTLSLPSTITHPP 316
           R +C  H    +  C+   K      +S+++LPS + +PP
Sbjct: 116 RNDCIFHDA--SGACHFFFKEVGNWTVSSIALPSNLANPP 153


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,304,592
Number of Sequences: 5004
Number of extensions: 68086
Number of successful extensions: 226
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 226
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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