BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00228 (622 letters) Database: mosquito 2352 sequences; 563,979 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha ... 27 0.36 AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha ... 25 2.6 >AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha 1 chain protein. Length = 1024 Score = 27.5 bits (58), Expect = 0.36 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = +1 Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGPQ 465 G E+G+RG + G+ G PGPQ Sbjct: 591 GPQGEKGDRGDSGLMGRPGNDGLPGPQ 617 Score = 24.6 bits (51), Expect = 2.6 Identities = 13/29 (44%), Positives = 16/29 (55%), Gaps = 3/29 (10%) Frame = +1 Query: 385 GSAAERGNRGRYDSLA---PEGDSGTPGP 462 G +RG+ G SL +GD GTPGP Sbjct: 122 GGMGDRGDPGLPGSLGYPGEKGDLGTPGP 150 Score = 24.2 bits (50), Expect = 3.4 Identities = 10/26 (38%), Positives = 12/26 (46%) Frame = +1 Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGP 462 G E G +GR + G G PGP Sbjct: 27 GDKGEMGEQGRTGAQGNAGPPGAPGP 52 Score = 23.4 bits (48), Expect = 5.9 Identities = 10/27 (37%), Positives = 12/27 (44%) Frame = +1 Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGPQ 465 G + G G P+G G PGPQ Sbjct: 600 GDSGLMGRPGNDGLPGPQGQRGLPGPQ 626 Score = 23.0 bits (47), Expect = 7.8 Identities = 9/25 (36%), Positives = 12/25 (48%) Frame = +1 Query: 385 GSAAERGNRGRYDSLAPEGDSGTPG 459 G+ RG +G + GD G PG Sbjct: 110 GNPGLRGPKGERGGMGDRGDPGLPG 134 Score = 23.0 bits (47), Expect = 7.8 Identities = 10/25 (40%), Positives = 12/25 (48%) Frame = +1 Query: 385 GSAAERGNRGRYDSLAPEGDSGTPG 459 G E+G+RG G SG PG Sbjct: 242 GEPGEKGDRGEIGVKGLMGQSGPPG 266 >AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha 1 chain precursor protein. Length = 801 Score = 24.6 bits (51), Expect = 2.6 Identities = 10/27 (37%), Positives = 14/27 (51%) Frame = +1 Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGPQ 465 G E+G RG+ +G G PGP+ Sbjct: 432 GFDGEKGERGQMGPKGGQGVPGRPGPE 458 Score = 24.2 bits (50), Expect = 3.4 Identities = 11/28 (39%), Positives = 14/28 (50%) Frame = +1 Query: 382 GGSAAERGNRGRYDSLAPEGDSGTPGPQ 465 G + A G GR + P+G G GPQ Sbjct: 398 GPAGAPGGGEGRPGAPGPKGPRGYEGPQ 425 Score = 23.8 bits (49), Expect = 4.5 Identities = 11/25 (44%), Positives = 12/25 (48%) Frame = +1 Query: 385 GSAAERGNRGRYDSLAPEGDSGTPG 459 G E G +G L P G SG PG Sbjct: 625 GLRGEPGPKGEPGLLGPPGPSGEPG 649 Score = 23.0 bits (47), Expect = 7.8 Identities = 10/27 (37%), Positives = 13/27 (48%) Frame = +1 Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGPQ 465 G A +RG G +G G PGP+ Sbjct: 323 GQAGDRGQVGERGHKGEKGLPGQPGPR 349 Score = 23.0 bits (47), Expect = 7.8 Identities = 19/71 (26%), Positives = 23/71 (32%), Gaps = 1/71 (1%) Frame = +1 Query: 250 KQADVLDIENSEEFEVDEDGEQXXXXXXXXXXXXXXXXXXNEAGG-GSAAERGNRGRYDS 426 K D D E E ++ G Q E+G G +G RG Sbjct: 428 KGMDGFDGEKGERGQMGPKGGQGVPGRPGPEGMPGDKGDKGESGSVGMPGPQGPRGYPGQ 487 Query: 427 LAPEGDSGTPG 459 PEG G PG Sbjct: 488 PGPEGLRGEPG 498 Score = 23.0 bits (47), Expect = 7.8 Identities = 10/27 (37%), Positives = 12/27 (44%) Frame = +1 Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGPQ 465 G E G+ G P G G PGP+ Sbjct: 465 GDKGESGSVGMPGPQGPRGYPGQPGPE 491 Database: mosquito Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 563,979 Number of sequences in database: 2352 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 366,307 Number of Sequences: 2352 Number of extensions: 5573 Number of successful extensions: 34 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 33 length of database: 563,979 effective HSP length: 62 effective length of database: 418,155 effective search space used: 60214320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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