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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00228
         (622 letters)

Database: mosquito 
           2352 sequences; 563,979 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha ...    27   0.36 
AY027891-1|AAK15783.1|  801|Anopheles gambiae collagen IV alpha ...    25   2.6  

>AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha 1
           chain protein.
          Length = 1024

 Score = 27.5 bits (58), Expect = 0.36
 Identities = 11/27 (40%), Positives = 15/27 (55%)
 Frame = +1

Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGPQ 465
           G   E+G+RG    +   G+ G PGPQ
Sbjct: 591 GPQGEKGDRGDSGLMGRPGNDGLPGPQ 617



 Score = 24.6 bits (51), Expect = 2.6
 Identities = 13/29 (44%), Positives = 16/29 (55%), Gaps = 3/29 (10%)
 Frame = +1

Query: 385 GSAAERGNRGRYDSLA---PEGDSGTPGP 462
           G   +RG+ G   SL     +GD GTPGP
Sbjct: 122 GGMGDRGDPGLPGSLGYPGEKGDLGTPGP 150



 Score = 24.2 bits (50), Expect = 3.4
 Identities = 10/26 (38%), Positives = 12/26 (46%)
 Frame = +1

Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGP 462
           G   E G +GR  +    G  G PGP
Sbjct: 27  GDKGEMGEQGRTGAQGNAGPPGAPGP 52



 Score = 23.4 bits (48), Expect = 5.9
 Identities = 10/27 (37%), Positives = 12/27 (44%)
 Frame = +1

Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGPQ 465
           G +   G  G      P+G  G PGPQ
Sbjct: 600 GDSGLMGRPGNDGLPGPQGQRGLPGPQ 626



 Score = 23.0 bits (47), Expect = 7.8
 Identities = 9/25 (36%), Positives = 12/25 (48%)
 Frame = +1

Query: 385 GSAAERGNRGRYDSLAPEGDSGTPG 459
           G+   RG +G    +   GD G PG
Sbjct: 110 GNPGLRGPKGERGGMGDRGDPGLPG 134



 Score = 23.0 bits (47), Expect = 7.8
 Identities = 10/25 (40%), Positives = 12/25 (48%)
 Frame = +1

Query: 385 GSAAERGNRGRYDSLAPEGDSGTPG 459
           G   E+G+RG        G SG PG
Sbjct: 242 GEPGEKGDRGEIGVKGLMGQSGPPG 266


>AY027891-1|AAK15783.1|  801|Anopheles gambiae collagen IV alpha 1
           chain precursor protein.
          Length = 801

 Score = 24.6 bits (51), Expect = 2.6
 Identities = 10/27 (37%), Positives = 14/27 (51%)
 Frame = +1

Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGPQ 465
           G   E+G RG+      +G  G PGP+
Sbjct: 432 GFDGEKGERGQMGPKGGQGVPGRPGPE 458



 Score = 24.2 bits (50), Expect = 3.4
 Identities = 11/28 (39%), Positives = 14/28 (50%)
 Frame = +1

Query: 382 GGSAAERGNRGRYDSLAPEGDSGTPGPQ 465
           G + A  G  GR  +  P+G  G  GPQ
Sbjct: 398 GPAGAPGGGEGRPGAPGPKGPRGYEGPQ 425



 Score = 23.8 bits (49), Expect = 4.5
 Identities = 11/25 (44%), Positives = 12/25 (48%)
 Frame = +1

Query: 385 GSAAERGNRGRYDSLAPEGDSGTPG 459
           G   E G +G    L P G SG PG
Sbjct: 625 GLRGEPGPKGEPGLLGPPGPSGEPG 649



 Score = 23.0 bits (47), Expect = 7.8
 Identities = 10/27 (37%), Positives = 13/27 (48%)
 Frame = +1

Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGPQ 465
           G A +RG  G       +G  G PGP+
Sbjct: 323 GQAGDRGQVGERGHKGEKGLPGQPGPR 349



 Score = 23.0 bits (47), Expect = 7.8
 Identities = 19/71 (26%), Positives = 23/71 (32%), Gaps = 1/71 (1%)
 Frame = +1

Query: 250 KQADVLDIENSEEFEVDEDGEQXXXXXXXXXXXXXXXXXXNEAGG-GSAAERGNRGRYDS 426
           K  D  D E  E  ++   G Q                   E+G  G    +G RG    
Sbjct: 428 KGMDGFDGEKGERGQMGPKGGQGVPGRPGPEGMPGDKGDKGESGSVGMPGPQGPRGYPGQ 487

Query: 427 LAPEGDSGTPG 459
             PEG  G PG
Sbjct: 488 PGPEGLRGEPG 498



 Score = 23.0 bits (47), Expect = 7.8
 Identities = 10/27 (37%), Positives = 12/27 (44%)
 Frame = +1

Query: 385 GSAAERGNRGRYDSLAPEGDSGTPGPQ 465
           G   E G+ G      P G  G PGP+
Sbjct: 465 GDKGESGSVGMPGPQGPRGYPGQPGPE 491


  Database: mosquito
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 563,979
  Number of sequences in database:  2352
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 366,307
Number of Sequences: 2352
Number of extensions: 5573
Number of successful extensions: 34
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33
length of database: 563,979
effective HSP length: 62
effective length of database: 418,155
effective search space used: 60214320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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