BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00222 (700 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAP7G5.05 |rpl1002|rpl10-2, rpl10|60S ribosomal protein L10|Sch... 179 4e-46 SPBC18E5.04 |rpl1001|rpl10-1, rpl10|60S ribosomal protein L10|Sc... 178 5e-46 SPAC19D5.03 |cid1||poly|Schizosaccharomyces pombe|chr 1|||Manual 29 0.64 SPCC1827.04 |||ankyrin repeat protein, unknown biological role|S... 27 2.0 SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2 |Schiz... 27 3.4 SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 26 4.5 SPBC1348.02 |||S. pombe specific 5Tm protein family|Schizosaccha... 26 6.0 SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.0 SPBPB2B2.19c |||S. pombe specific 5Tm protein family|Schizosacch... 26 6.0 SPAC977.01 |||S. pombe specific 5Tm protein family|Schizosacchar... 26 6.0 SPAC750.05c |||S. pombe specific 5Tm protein family|Schizosaccha... 26 6.0 SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 25 7.9 SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 25 7.9 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 7.9 SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8 |Schizo... 25 7.9 SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|... 25 7.9 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 25 7.9 >SPAP7G5.05 |rpl1002|rpl10-2, rpl10|60S ribosomal protein L10|Schizosaccharomyces pombe|chr 1|||Manual Length = 221 Score = 179 bits (435), Expect = 4e-46 Identities = 101/215 (46%), Positives = 127/215 (59%), Gaps = 10/215 (4%) Frame = +3 Query: 21 MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 200 M RRPARCYRYCKNKPYPKSR+ R VPD KIRIFDLG+KRA VD+FPLC+HLVS+EYEQ+ Sbjct: 1 MARRPARCYRYCKNKPYPKSRYNRAVPDSKIRIFDLGRKRAGVDEFPLCIHLVSNEYEQI 60 Query: 201 SSEALEAGRICCNKYLVKTAERIS---SISA*DFTLSTLSASIKCYHALEL-------IG 350 +SEALEA RIC NKYLVK + S + A F + ++ + C A L G Sbjct: 61 TSEALEAARICANKYLVKIGGKDSFHLRVRAHPFHVVRINKMLSCAGADRLQTGMRHAFG 120 Query: 351 SRLGCVVRLASLRVL*HVFALDSPSCPCALVTGGRHRSSRLCAVPSQVPRTSKIYVSKKW 530 G V R+ +VL V DS + R C + P +I VSKKW Sbjct: 121 KPNGLVARVNIGQVLMSVRTKDSSRATAI-------EALRRCQY--KFPGQQRIIVSKKW 171 Query: 531 GFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 635 GF++Y RDE+ + R G + DGC ++ + G L Sbjct: 172 GFSQYARDEYIEKRSRGEIIPDGCYAKFLNKRGSL 206 >SPBC18E5.04 |rpl1001|rpl10-1, rpl10|60S ribosomal protein L10|Schizosaccharomyces pombe|chr 2|||Manual Length = 221 Score = 178 bits (434), Expect = 5e-46 Identities = 100/215 (46%), Positives = 127/215 (59%), Gaps = 10/215 (4%) Frame = +3 Query: 21 MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 200 M RRPARCYRYCKNKPYPKSR+ R VPD KIRIFDLG+KRA VD+FPLC+HLVS+EYEQ+ Sbjct: 1 MARRPARCYRYCKNKPYPKSRYNRAVPDSKIRIFDLGRKRAGVDEFPLCIHLVSNEYEQI 60 Query: 201 SSEALEAGRICCNKYLVKTAERIS---SISA*DFTLSTLSASIKCYHALEL-------IG 350 +SEALEA RIC NKYLVK + S + A F + ++ + C A L G Sbjct: 61 TSEALEAARICANKYLVKIGGKDSFHLRVRAHPFHVVRINKMLSCAGADRLQTGMRHAFG 120 Query: 351 SRLGCVVRLASLRVL*HVFALDSPSCPCALVTGGRHRSSRLCAVPSQVPRTSKIYVSKKW 530 G V R+ ++L V DS + R C + P +I VSKKW Sbjct: 121 KPNGLVARVNIGQILMSVRTKDSSRATAI-------EALRRCQY--KFPGQQRIIVSKKW 171 Query: 531 GFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 635 GF++Y RDE+ + R G + DGC ++ + G L Sbjct: 172 GFSQYARDEYIEKRSRGEIIPDGCYAKFLNKRGSL 206 >SPAC19D5.03 |cid1||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 405 Score = 29.1 bits (62), Expect = 0.64 Identities = 18/61 (29%), Positives = 32/61 (52%), Gaps = 1/61 (1%) Frame = +3 Query: 45 YRYCKNKPYPKSRFCRGVPDP-KIRIFDLGKKRATVDDFPLCVHLVSDEYEQLSSEALEA 221 Y+ N ++FC V + KI + +KRA +D LC+ +S + E ++ +LE+ Sbjct: 34 YQKVPNSHKEFTKFCYEVYNEIKISDKEFKEKRAALDTLRLCLKRISPDAELVAFGSLES 93 Query: 222 G 224 G Sbjct: 94 G 94 >SPCC1827.04 |||ankyrin repeat protein, unknown biological role|Schizosaccharomyces pombe|chr 3|||Manual Length = 600 Score = 27.5 bits (58), Expect = 2.0 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Frame = +3 Query: 102 DPKIRIFDLGKKRATVDDFPLCV-HLVSDEYEQLSSEALEA 221 DPK+R+F KRAT + C LV+ + + S +++A Sbjct: 320 DPKLRVFPFTTKRATHSELLRCYKELVTPKISHVDSISIKA 360 >SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1273 Score = 26.6 bits (56), Expect = 3.4 Identities = 12/24 (50%), Positives = 13/24 (54%) Frame = +3 Query: 228 ICCNKYLVKTAERISSISA*DFTL 299 ICC KY V T R + I FTL Sbjct: 9 ICCAKYFVNTKSRFNEILPPRFTL 32 >SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 26.2 bits (55), Expect = 4.5 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = +1 Query: 136 RERPLTTFHCACTWCPTNMNS 198 R RP +T H +CT+C + S Sbjct: 459 RSRPDSTSHASCTYCVARLRS 479 >SPBC1348.02 |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 2|||Manual Length = 344 Score = 25.8 bits (54), Expect = 6.0 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = -3 Query: 590 SEAALFTQLLKLITFILCETPLL*YV 513 S+A F ++L FI C +P+L Y+ Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHYL 288 >SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1038 Score = 25.8 bits (54), Expect = 6.0 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = -1 Query: 394 STLRLAKRTTHPSLEPISSS 335 +T +LAKR T PSL P S+ Sbjct: 957 TTTKLAKRITQPSLSPARST 976 >SPBPB2B2.19c |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 2|||Manual Length = 344 Score = 25.8 bits (54), Expect = 6.0 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = -3 Query: 590 SEAALFTQLLKLITFILCETPLL*YV 513 S+A F ++L FI C +P+L Y+ Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHYL 288 >SPAC977.01 |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 316 Score = 25.8 bits (54), Expect = 6.0 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = -3 Query: 590 SEAALFTQLLKLITFILCETPLL*YV 513 S+A F ++L FI C +P+L Y+ Sbjct: 235 SKARFFRRMLNAFIFIFCASPMLHYL 260 >SPAC750.05c |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 1|||Manual Length = 344 Score = 25.8 bits (54), Expect = 6.0 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = -3 Query: 590 SEAALFTQLLKLITFILCETPLL*YV 513 S+A F ++L FI C +P+L Y+ Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHYL 288 >SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 2685 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/38 (31%), Positives = 19/38 (50%) Frame = -3 Query: 134 LTQIEDTDLGIRYTPTEPRFRIRFIFAVPVASCWPAPH 21 L Q++++ L + T T P +R V SC+P H Sbjct: 408 LHQLQNSILRVNSTLTSPSLDLRLELLHDVLSCFPKKH 445 >SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr 1|||Manual Length = 1234 Score = 25.4 bits (53), Expect = 7.9 Identities = 11/39 (28%), Positives = 21/39 (53%) Frame = -3 Query: 221 CLQSL*AQLFIFVGHQVHAQWKVVNGRSLLTQIEDTDLG 105 CL L L + H++ ++W+ +N R LL + + + G Sbjct: 940 CLLPLLPALLSELDHKLVSEWRRINDRGLLVEEDAEETG 978 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 25.4 bits (53), Expect = 7.9 Identities = 14/35 (40%), Positives = 20/35 (57%) Frame = +2 Query: 263 KDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMR 367 +D H+RM ++ FH IN MLS + D + MR Sbjct: 859 EDNSHLRMSINSFH---INDMLSKSSRDNNRQLMR 890 >SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8 |Schizosaccharomyces pombe|chr 3|||Manual Length = 569 Score = 25.4 bits (53), Expect = 7.9 Identities = 20/75 (26%), Positives = 32/75 (42%), Gaps = 8/75 (10%) Frame = -2 Query: 237 CSRYVLPP-KP-LSSAVHIRRTPSARTVESRQRS------LSSYPNRRYGSWDQVHPDRT 82 C++ + PP KP + SA+ S T E S LS +R+ +W P Sbjct: 494 CAKKIQPPFKPSVESAIDTSNFDSEFTSEIPMDSVVADSHLSETVQQRFANWSYQRPTTI 553 Query: 81 SISDTVYFCSTGSIL 37 SD + + GS++ Sbjct: 554 DTSDDINTIAPGSVI 568 >SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1174 Score = 25.4 bits (53), Expect = 7.9 Identities = 8/15 (53%), Positives = 13/15 (86%) Frame = +1 Query: 88 VGVYLIPRSVSSIWV 132 +GVYLIP++ S +W+ Sbjct: 394 IGVYLIPKAWSDLWL 408 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = +3 Query: 123 DLGKKRATVDDFPLCVHLVSDEYEQL 200 ++ KKRA DD + +VS EYE L Sbjct: 1033 EIEKKRAENDDLQSKLSVVSSEYENL 1058 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,131,161 Number of Sequences: 5004 Number of extensions: 67603 Number of successful extensions: 230 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 217 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 224 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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