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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00203
         (705 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g30775.1 68416.m03933 proline oxidase, mitochondrial / osmoti...    52   3e-07
At5g38710.1 68418.m04682 proline oxidase, putative / osmotic str...    38   0.009
At5g38200.1 68418.m04605 expressed protein                             29   4.0  
At1g68890.1 68414.m07884 menaquinone biosynthesis protein-relate...    29   4.0  

>At3g30775.1 68416.m03933 proline oxidase, mitochondrial / osmotic
           stress-responsive proline dehydrogenase (POX) (PRO1)
           (ERD5) nearly identical to SP|P92983 Proline oxidase,
           mitochondrial precursor (EC 1.5.3.-) (Osmotic stress-
           induced proline dehydrogenase) [Arabidopsis thaliana];
           identical to cDNA proline oxidase precursor GI:1817543
          Length = 499

 Score = 52.4 bits (120), Expect = 3e-07
 Identities = 24/84 (28%), Positives = 50/84 (59%)
 Frame = +3

Query: 255 ENVDATTASFHKCLKEILSRVKSGKTGLGIMVASHNEDTVRYAIQLMKEHNIKPEDKVVC 434
           + +  T + ++ C+  ++ +  +G +G G+++A+HN D+ R A +   +  I  ++  + 
Sbjct: 369 DTIQDTHSCYNDCMTFLMEKASNG-SGFGVVLATHNADSGRLASRKASDLGIDKQNGKIE 427

Query: 435 FGQLLGMCDHITFPLGQAGYSVYK 506
           F QL GM D ++F L +AG++V K
Sbjct: 428 FAQLYGMSDALSFGLKRAGFNVSK 451



 Score = 38.3 bits (85), Expect = 0.005
 Identities = 21/64 (32%), Positives = 33/64 (51%), Gaps = 2/64 (3%)
 Frame = +2

Query: 2   DAEQTYFQPAISRICLEMMRRYN--KDKFLVFNTYQTYLKNTYNEIVTDLEQAHRQNFFW 175
           DAE T  QPAI  +       +N  KD+ +V+NT Q YL++    +   ++ A ++N   
Sbjct: 282 DAEDTILQPAIDYMAYSSAIMFNADKDRPIVYNTIQAYLRDAGERLHLAVQNAEKENVPM 341

Query: 176 GAXL 187
           G  L
Sbjct: 342 GFKL 345



 Score = 35.9 bits (79), Expect = 0.026
 Identities = 14/22 (63%), Positives = 17/22 (77%)
 Frame = +2

Query: 509 VPYGPVVEVLPYLSRRANENRG 574
           +P+GPV   +PYL RRA ENRG
Sbjct: 453 MPFGPVATAIPYLLRRAYENRG 474


>At5g38710.1 68418.m04682 proline oxidase, putative / osmotic
           stress-responsive proline dehydrogenase, putative
           similar to proline oxidase, mitochondrial precursor
           (Osmotic stress- induced proline dehydrogenase)
           [Arabidopsis thaliana] SWISS-PROT:P92983
          Length = 476

 Score = 37.5 bits (83), Expect = 0.009
 Identities = 20/57 (35%), Positives = 31/57 (54%), Gaps = 2/57 (3%)
 Frame = +2

Query: 2   DAEQTYFQPAISRICL--EMMRRYNKDKFLVFNTYQTYLKNTYNEIVTDLEQAHRQN 166
           DAE T  QPAI  +     +M   +KD+ +V+NT Q YLK+    +   L ++ + N
Sbjct: 261 DAEDTILQPAIDYMAYWSAIMFNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMN 317



 Score = 36.3 bits (80), Expect = 0.020
 Identities = 15/22 (68%), Positives = 17/22 (77%)
 Frame = +2

Query: 509 VPYGPVVEVLPYLSRRANENRG 574
           +PYGPV   +PYL RRA ENRG
Sbjct: 432 MPYGPVDTAIPYLIRRAYENRG 453


>At5g38200.1 68418.m04605 expressed protein
          Length = 431

 Score = 28.7 bits (61), Expect = 4.0
 Identities = 21/73 (28%), Positives = 39/73 (53%), Gaps = 1/73 (1%)
 Frame = +3

Query: 252 VENVDATTASFHKCLKEILSRVKSGKTGLGIMVASHNEDTVRYAIQLMKEHNIKPEDKVV 431
           +E+  A +A     LKE+ + V++G  G  +     +ED  R A  +MK+ NI+   +++
Sbjct: 345 LESNTALSAEQEMRLKEMGATVRNG--GSYMKKLKVDEDKQRMARNMMKKMNIEQLSELM 402

Query: 432 CFGQLLG-MCDHI 467
            F  L+G +C  +
Sbjct: 403 AFYHLMGNICGEV 415


>At1g68890.1 68414.m07884 menaquinone biosynthesis protein-related
           similar to menaquinone biosynthesis protein menD
           [SP|P17109][E. coli] ; Pfam HMM hit:
           2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic
           acid synthase / 2-oxoglutarate decarboxylase
          Length = 894

 Score = 28.7 bits (61), Expect = 4.0
 Identities = 14/36 (38%), Positives = 21/36 (58%)
 Frame = +3

Query: 294 LKEILSRVKSGKTGLGIMVASHNEDTVRYAIQLMKE 401
           + EIL  +K  K GL ++ A H ED +  ++ L KE
Sbjct: 554 ITEILQVIKEAKKGLLLIGAIHTEDEIWASLLLAKE 589


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,858,226
Number of Sequences: 28952
Number of extensions: 303345
Number of successful extensions: 833
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 808
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 831
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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