Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00201
(705 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 121 9e-29
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 121 9e-29
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 99 3e-22
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 74 2e-14
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 32 0.070
SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase |Schizosa... 27 2.6
SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 27 3.5
SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 27 3.5
SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe... 27 3.5
SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 26 4.6
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 26 4.6
SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 26 4.6
SPAC23H4.16c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 8.0
SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom... 25 8.0
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 121 bits (292), Expect = 9e-29
Identities = 59/84 (70%), Positives = 63/84 (75%)
Frame = +2
Query: 257 LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYA 436
LNEIKDSVVA F WA+KEG M EENLR RFNI DV LH DAIHRGGGQIIPT RR +YA
Sbjct: 656 LNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYA 715
Query: 437 CLLTAQPRLMEPVYLCEIQCPEVA 508
L A P + EPV+L EIQ E A
Sbjct: 716 STLLASPIIQEPVFLVEIQVSENA 739
Score = 116 bits (279), Expect = 3e-27
Identities = 53/109 (48%), Positives = 72/109 (66%), Gaps = 5/109 (4%)
Frame = +3
Query: 3 SDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEA 182
S LSKSPNKHNR+FM A+PM + L I+ G VNPRDDFK RAR + +++ +DVT+A
Sbjct: 571 SSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDA 630
Query: 183 RKIWCFGPEGTGPNILVDCSKGVQTSMKLR-----TLLWLDSSGPLRKE 314
RKIWCFGP+ TG N++VD +K V +++ W GP+ +E
Sbjct: 631 RKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEE 679
Score = 104 bits (250), Expect = 1e-23
Identities = 44/61 (72%), Positives = 50/61 (81%)
Frame = +1
Query: 511 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDH 690
GGIY VLN++RGHVF E Q GTP++ +KAYLPVNESFGFT +LR T GQAFPQ VFDH
Sbjct: 741 GGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQATAGQAFPQLVFDH 800
Query: 691 W 693
W
Sbjct: 801 W 801
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 121 bits (292), Expect = 9e-29
Identities = 59/84 (70%), Positives = 63/84 (75%)
Frame = +2
Query: 257 LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYA 436
LNEIKDSVVA F WA+KEG M EENLR RFNI DV LH DAIHRGGGQIIPT RR +YA
Sbjct: 656 LNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYA 715
Query: 437 CLLTAQPRLMEPVYLCEIQCPEVA 508
L A P + EPV+L EIQ E A
Sbjct: 716 STLLASPIIQEPVFLVEIQVSENA 739
Score = 116 bits (279), Expect = 3e-27
Identities = 53/109 (48%), Positives = 72/109 (66%), Gaps = 5/109 (4%)
Frame = +3
Query: 3 SDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEA 182
S LSKSPNKHNR+FM A+PM + L I+ G VNPRDDFK RAR + +++ +DVT+A
Sbjct: 571 SSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDA 630
Query: 183 RKIWCFGPEGTGPNILVDCSKGVQTSMKLR-----TLLWLDSSGPLRKE 314
RKIWCFGP+ TG N++VD +K V +++ W GP+ +E
Sbjct: 631 RKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEE 679
Score = 104 bits (250), Expect = 1e-23
Identities = 44/61 (72%), Positives = 50/61 (81%)
Frame = +1
Query: 511 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDH 690
GGIY VLN++RGHVF E Q GTP++ +KAYLPVNESFGFT +LR T GQAFPQ VFDH
Sbjct: 741 GGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQATAGQAFPQLVFDH 800
Query: 691 W 693
W
Sbjct: 801 W 801
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 99 bits (238), Expect = 3e-22
Identities = 42/81 (51%), Positives = 55/81 (67%)
Frame = +2
Query: 257 LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYA 436
LN +K+ + GFQW +EG + +E +R V F + DV L + I+RGGGQIIPT RR Y+
Sbjct: 772 LNSVKEYIKQGFQWGTREGPLCDETIRNVNFRLMDVVLAPEQIYRGGGQIIPTARRVCYS 831
Query: 437 CLLTAQPRLMEPVYLCEIQCP 499
LTA PRLMEPVY+ E+ P
Sbjct: 832 SFLTASPRLMEPVYMVEVHAP 852
Score = 78.6 bits (185), Expect = 8e-16
Identities = 34/63 (53%), Positives = 46/63 (73%)
Frame = +1
Query: 517 IYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQ 696
IY +L RRRGHV ++ G+P+++V+A +PV +S GF DLR +T GQA Q VFDHWQ
Sbjct: 859 IYDLLTRRRGHVLQDIPRPGSPLYLVRALIPVIDSCGFETDLRVHTQGQAMCQMVFDHWQ 918
Query: 697 VLP 705
V+P
Sbjct: 919 VVP 921
Score = 69.7 bits (163), Expect = 4e-13
Identities = 38/109 (34%), Positives = 55/109 (50%), Gaps = 9/109 (8%)
Frame = +3
Query: 15 CLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIW 194
C S +PNK NR+ M +P+ G+ DI+ G+VN K + + + Y++D+ +R IW
Sbjct: 687 CFSDTPNKKNRITMVVEPLEKGISNDIENGKVNINWPQKRISEFFQKNYDWDLLASRSIW 746
Query: 195 CFGPEGTGPNIL------VDCSKGVQTSMK---LRTLLWLDSSGPLRKE 314
FGP+ G NIL D K V S+K + W GPL E
Sbjct: 747 AFGPDDRGTNILRDDTLSTDVDKNVLNSVKEYIKQGFQWGTREGPLCDE 795
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1000
Score = 73.7 bits (173), Expect = 2e-14
Identities = 31/64 (48%), Positives = 44/64 (68%)
Frame = +1
Query: 511 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDH 690
G +YGV+++RRG V +E GTP FIVKA +PV ESFGF ++ T G A+PQ +F
Sbjct: 879 GRVYGVVSKRRGRVIDEEMKEGTPFFIVKALIPVVESFGFAVEILKRTSGAAYPQLIFHG 938
Query: 691 WQVL 702
+++L
Sbjct: 939 FEML 942
Score = 30.3 bits (65), Expect = 0.28
Identities = 21/87 (24%), Positives = 41/87 (47%), Gaps = 9/87 (10%)
Frame = +2
Query: 260 NEIKDSVVAGFQWAAKEGVMAEENLRGV-----RFNIYDVTLHTDAIHRGG----GQIIP 412
+++ + VV FQ +G + E ++G+ +F+I D + + + GQ+I
Sbjct: 786 SDLSEYVVTAFQLITHQGPLCAEPVQGICVSIDQFDISDDSEDSKLLTINNPQIPGQVIS 845
Query: 413 TTRRCLYACLLTAQPRLMEPVYLCEIQ 493
+ + L PRLM +Y C++Q
Sbjct: 846 VVKESIRHGFLGWSPRLMLAMYSCDVQ 872
Score = 27.5 bits (58), Expect = 2.0
Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 4/46 (8%)
Frame = +3
Query: 120 DDFKTRARYLTEKYEYDVTEARK----IWCFGPEGTGPNILVDCSK 245
+ F R L E+ D+ E + I FGP+ GPNIL D +K
Sbjct: 724 EKFYERLSKLLEEENSDLGELKNHLDSIIAFGPKRVGPNILFDKTK 769
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 32.3 bits (70), Expect = 0.070
Identities = 14/51 (27%), Positives = 30/51 (58%)
Frame = +1
Query: 511 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 663
GG+ G L++R+ + + F ++A +P+N F +++D+R+ T G+
Sbjct: 691 GGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFSYSSDIRALTKGK 739
>SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 27.1 bits (57), Expect = 2.6
Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 2/52 (3%)
Frame = -3
Query: 301 GPLESSHNR--VLNFIEV*TPLEQSTRMLGPVPSGPKHQILRASVTSYSYFS 152
G L SSHN V+ + E+ ++T + G KH +LRA+ +Y YFS
Sbjct: 288 GLLVSSHNHPCVVEYSEISDEACKATENV----DGHKHLLLRAANIAYHYFS 335
>SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 391
Score = 26.6 bits (56), Expect = 3.5
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = -3
Query: 472 RLHETGL-SS*QTCVQASSSCWNDLATTSMDGISMECYIIDVESNTTQI 329
++ ET L ++ Q + CW++L+TTS + + II + TT I
Sbjct: 191 QIQETSLGATTQEDESSDGICWDELSTTSPESSKVSEPIIQDNTQTTHI 239
>SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase
Cdc48|Schizosaccharomyces pombe|chr 1|||Manual
Length = 815
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/46 (26%), Positives = 23/46 (50%)
Frame = +3
Query: 93 IDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPNIL 230
++E + R+ + Y + + VT ++ + FGP GTG +L
Sbjct: 502 LEEVKRELRETVQMPVMYAEKFLRFGVTPSKGVLFFGPPGTGKTLL 547
>SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 230
Score = 26.6 bits (56), Expect = 3.5
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2
Query: 209 LGAKAPNLTGFGNIVFVLFCKIPSAS 132
+G PNL GN +F L ++PS+S
Sbjct: 169 IGILPPNLNSIGNKMFSLKSRVPSSS 194
>SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+,
L-lysine forming] |Schizosaccharomyces pombe|chr
1|||Manual
Length = 368
Score = 26.2 bits (55), Expect = 4.6
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = -1
Query: 417 VVGMIWPPPLWMASVWSVTS*MLNLTPRKFSSAITPSLAA 298
V G+ PPPL + S+ + + + + FS A+ PSL A
Sbjct: 305 VKGVTTPPPLEVISIDHLPTLLPRESSEAFSEALIPSLLA 344
>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1253
Score = 26.2 bits (55), Expect = 4.6
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +3
Query: 81 LPEDIDEGRVNPRDDFKT 134
LPE IDEG+++ DD KT
Sbjct: 194 LPEKIDEGKLSDVDDGKT 211
>SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 871
Score = 26.2 bits (55), Expect = 4.6
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +3
Query: 18 LSKSPNKHNRLFMKAQPMPDGL 83
++KS KHN+L K +P+P L
Sbjct: 243 INKSVKKHNKLVKKHKPLPSTL 264
>SPAC23H4.16c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 328
Score = 25.4 bits (53), Expect = 8.0
Identities = 23/83 (27%), Positives = 39/83 (46%), Gaps = 7/83 (8%)
Frame = -3
Query: 493 LNFTKIYRLHETGLSS*QTCVQASSSCWND-----LAT--TSMDGISMECYIIDVESNTT 335
L+++K++ LH +Q S S +ND LA ++ + + Y++ E +
Sbjct: 192 LSYSKVFFLH---------LLQHSDSSFNDKFLLLLANIPVTVSNVEVLLYLLQQEESLA 242
Query: 334 QILFSHNSFLSGPLESSHNRVLN 266
Q + SFL L S HN+V N
Sbjct: 243 QFDLNGKSFLYHMLVSLHNQVTN 265
>SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 166
Score = 25.4 bits (53), Expect = 8.0
Identities = 12/42 (28%), Positives = 21/42 (50%)
Frame = -1
Query: 393 PLWMASVWSVTS*MLNLTPRKFSSAITPSLAAHWNPATTESL 268
P W+A V ++ S + P FSS + +L A+ N + +
Sbjct: 43 PFWLAEVLAINSFVSIHMPAPFSSVVRNALKANPNSVSIRDI 84
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,189,682
Number of Sequences: 5004
Number of extensions: 69840
Number of successful extensions: 210
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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