Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00201
(705 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 135 2e-32
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 98 4e-21
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 98 4e-21
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 98 4e-21
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 66 2e-11
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 33 0.14
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 33 0.14
At1g67560.1 68414.m07697 lipoxygenase family protein similar to ... 29 3.0
At3g57420.1 68416.m06393 expressed protein contains Pfam domain ... 29 4.0
At3g53160.1 68416.m05858 UDP-glucoronosyl/UDP-glucosyl transfera... 29 4.0
At5g23240.1 68418.m02719 DNAJ heat shock N-terminal domain-conta... 28 5.2
At5g15580.1 68418.m01824 expressed protein unknown protein F14P3... 28 5.2
At5g26210.1 68418.m03119 PHD finger family protein contains Pfam... 28 6.9
At2g41770.1 68415.m05163 expressed protein contains Pfam domain ... 28 6.9
At5g26920.1 68418.m03210 calmodulin-binding protein similar to c... 27 9.2
At3g63180.1 68416.m07097 expressed protein 27 9.2
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 135 bits (327), Expect = 2e-32
Identities = 60/84 (71%), Positives = 72/84 (85%)
Frame = +2
Query: 257 LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYA 436
LNEIKDSVVAGFQWA+KEG +AEEN+RG+ F + DV LH+DAIHRGGGQ+IPT RR +YA
Sbjct: 657 LNEIKDSVVAGFQWASKEGPLAEENMRGICFEVCDVVLHSDAIHRGGGQVIPTARRVIYA 716
Query: 437 CLLTAQPRLMEPVYLCEIQCPEVA 508
+TA+PRL+EPVY+ EIQ PE A
Sbjct: 717 SQITAKPRLLEPVYMVEIQAPEGA 740
Score = 116 bits (280), Expect = 1e-26
Identities = 56/116 (48%), Positives = 79/116 (68%), Gaps = 5/116 (4%)
Frame = +3
Query: 3 SDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEA 182
S + +SKSPNKHNRL+M+A+PM +GL E ID+GR+ PRDD K R++ L E++ +D A
Sbjct: 572 STRTVMSKSPNKHNRLYMEARPMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWDKDLA 631
Query: 183 RKIWCFGPEGTGPNILVDCSKGVQTSMKLRTLL-----WLDSSGPLRKELWLKRIC 335
+KIW FGPE TGPN++VD KGVQ +++ + W GPL +E ++ IC
Sbjct: 632 KKIWAFGPETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGPLAEEN-MRGIC 686
Score = 106 bits (254), Expect = 2e-23
Identities = 44/64 (68%), Positives = 55/64 (85%)
Frame = +1
Query: 511 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDH 690
GGIY VLN++RGHVFEE Q GTP++ +KAYLPV ESFGF++ LR+ T GQAFPQCVFDH
Sbjct: 742 GGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSQLRAATSGQAFPQCVFDH 801
Query: 691 WQVL 702
W+++
Sbjct: 802 WEMM 805
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 98.3 bits (234), Expect = 4e-21
Identities = 42/78 (53%), Positives = 54/78 (69%)
Frame = +2
Query: 266 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 445
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 762 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 821
Query: 446 TAQPRLMEPVYLCEIQCP 499
A PRLMEPVY EIQ P
Sbjct: 822 MATPRLMEPVYYVEIQTP 839
Score = 82.2 bits (194), Expect = 3e-16
Identities = 38/63 (60%), Positives = 46/63 (73%)
Frame = +1
Query: 517 IYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQ 696
IY VL+RRRG+V + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW
Sbjct: 846 IYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWA 905
Query: 697 VLP 705
++P
Sbjct: 906 IVP 908
Score = 68.9 bits (161), Expect = 3e-12
Identities = 32/78 (41%), Positives = 47/78 (60%)
Frame = +3
Query: 3 SDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEA 182
S C +++PNK N+L M A+P+ GL EDI+ G V+ + + KY++D+ A
Sbjct: 670 SSMKCFAETPNKKNKLTMIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAA 729
Query: 183 RKIWCFGPEGTGPNILVD 236
R IW FGP+ G NIL+D
Sbjct: 730 RSIWAFGPDKQGTNILLD 747
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 98.3 bits (234), Expect = 4e-21
Identities = 42/78 (53%), Positives = 54/78 (69%)
Frame = +2
Query: 266 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 445
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 776 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 835
Query: 446 TAQPRLMEPVYLCEIQCP 499
A PRLMEPVY EIQ P
Sbjct: 836 MATPRLMEPVYYVEIQTP 853
Score = 84.6 bits (200), Expect = 6e-17
Identities = 39/63 (61%), Positives = 46/63 (73%)
Frame = +1
Query: 517 IYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQ 696
IY VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW
Sbjct: 860 IYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWA 919
Query: 697 VLP 705
++P
Sbjct: 920 IVP 922
Score = 74.1 bits (174), Expect = 8e-14
Identities = 33/78 (42%), Positives = 49/78 (62%)
Frame = +3
Query: 3 SDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEA 182
S C +++PNK N++ M A+P+ GL EDI+ G V+ + K + KY++D+ A
Sbjct: 684 SSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAA 743
Query: 183 RKIWCFGPEGTGPNILVD 236
R IW FGP+ GPNIL+D
Sbjct: 744 RSIWAFGPDKQGPNILLD 761
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 98.3 bits (234), Expect = 4e-21
Identities = 42/78 (53%), Positives = 54/78 (69%)
Frame = +2
Query: 266 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 445
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 776 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 835
Query: 446 TAQPRLMEPVYLCEIQCP 499
A PRLMEPVY EIQ P
Sbjct: 836 MATPRLMEPVYYVEIQTP 853
Score = 84.6 bits (200), Expect = 6e-17
Identities = 39/63 (61%), Positives = 46/63 (73%)
Frame = +1
Query: 517 IYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQ 696
IY VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW
Sbjct: 860 IYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWA 919
Query: 697 VLP 705
++P
Sbjct: 920 IVP 922
Score = 74.1 bits (174), Expect = 8e-14
Identities = 33/78 (42%), Positives = 49/78 (62%)
Frame = +3
Query: 3 SDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEA 182
S C +++PNK N++ M A+P+ GL EDI+ G V+ + K + KY++D+ A
Sbjct: 684 SSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAA 743
Query: 183 RKIWCFGPEGTGPNILVD 236
R IW FGP+ GPNIL+D
Sbjct: 744 RSIWAFGPDKQGPNILLD 761
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 66.5 bits (155), Expect = 2e-11
Identities = 29/64 (45%), Positives = 40/64 (62%)
Frame = +1
Query: 511 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDH 690
G +Y VL+RRR + +E G+ +F V AY+PV+ESFGF +LR T G A V H
Sbjct: 891 GPMYAVLSRRRARILKEEMQEGSSLFTVHAYVPVSESFGFADELRKGTSGGASALMVLSH 950
Query: 691 WQVL 702
W++L
Sbjct: 951 WEML 954
Score = 42.3 bits (95), Expect = 3e-04
Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 9/93 (9%)
Frame = +2
Query: 251 SDLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYD-----VTLHTDAIHRGG---GQI 406
S+ ++ S+V+GFQ A G + +E + G+ F I + TD G GQ+
Sbjct: 796 SEALTLESSIVSGFQLATASGPLCDEPMWGLAFTIESHLAPAEDVETDKPENFGIFTGQV 855
Query: 407 IPTTRRCLYACLLTAQPRLMEPVYLCEIQ-CPE 502
+ + A +L PR++E +Y CE+ PE
Sbjct: 856 MTAVKDACRAAVLQTNPRIVEAMYFCELNTAPE 888
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 33.5 bits (73), Expect = 0.14
Identities = 16/51 (31%), Positives = 28/51 (54%)
Frame = +1
Query: 511 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 663
G + G +N+R+G + Q ++ A +P+N FG++ LRS T G+
Sbjct: 674 GTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQGK 722
Score = 27.9 bits (59), Expect = 6.9
Identities = 20/78 (25%), Positives = 34/78 (43%), Gaps = 3/78 (3%)
Frame = +2
Query: 275 SVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYA---CLL 445
++ GF+ AA G + + +R + L A H + +YA C
Sbjct: 597 AIEKGFKEAANSGSLIGHPVENLR-----IVLTDGASHAVDSSELAFKMAAIYAFRLCYT 651
Query: 446 TAQPRLMEPVYLCEIQCP 499
A+P ++EPV L E++ P
Sbjct: 652 AARPVILEPVMLVELKVP 669
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 33.5 bits (73), Expect = 0.14
Identities = 16/51 (31%), Positives = 28/51 (54%)
Frame = +1
Query: 511 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 663
G + G +N+R+G + Q ++ A +P+N FG++ LRS T G+
Sbjct: 674 GTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQGK 722
Score = 27.9 bits (59), Expect = 6.9
Identities = 20/78 (25%), Positives = 34/78 (43%), Gaps = 3/78 (3%)
Frame = +2
Query: 275 SVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYA---CLL 445
++ GF+ AA G + + +R + L A H + +YA C
Sbjct: 597 AIEKGFKEAANSGSLIGHPVENLR-----IVLTDGASHAVDSSELAFKMAAIYAFRLCYT 651
Query: 446 TAQPRLMEPVYLCEIQCP 499
A+P ++EPV L E++ P
Sbjct: 652 AARPVILEPVMLVELKVP 669
>At1g67560.1 68414.m07697 lipoxygenase family protein similar to
13-lipoxygenase GB:CAA65269 [Solanum tuberosum],
gi:1654140 [Lycopersicon esculentum]
Length = 917
Score = 29.1 bits (62), Expect = 3.0
Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +3
Query: 21 SKSPNKHNRLFMKAQPM-----PDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDV 173
SK+ N R+ ++QP PDG+ E ++ V+ R D K + Y+YDV
Sbjct: 204 SKNDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGERKPHERIYDYDV 259
>At3g57420.1 68416.m06393 expressed protein contains Pfam domain
PF03385: Protein of unknown function, DUF288
Length = 765
Score = 28.7 bits (61), Expect = 4.0
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = -2
Query: 284 PQQSP*FH*GLNSFGAIHQDVGAGTLGAKAPNLTGFGNIVFVLFCKIP 141
P++ P H G+ G + ++G K FGN+V ++FC P
Sbjct: 480 PRKLPSVHLGVEEIGTVSSEIGNLIKWRK-----NFGNVVLIMFCNGP 522
>At3g53160.1 68416.m05858 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 490
Score = 28.7 bits (61), Expect = 4.0
Identities = 17/46 (36%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
Frame = +3
Query: 162 EYDVTEARKIWCFGPEGTGPNILVDCSK-GVQTSM-KLRTLLWLDS 293
EY A K+WC GP + +D +K G + S+ + + L WLDS
Sbjct: 233 EYRKARAGKVWCVGPVSLCNRLGLDKAKRGDKASIGQDQCLQWLDS 278
>At5g23240.1 68418.m02719 DNAJ heat shock N-terminal
domain-containing protein low similarity to SP|O34136
Chaperone protein dnaJ (40 kDa heat shock chaperone
protein) (HSP40) {Deinococcus proteolyticus}; contains
Pfam profile PF00226: DnaJ domain
Length = 465
Score = 28.3 bits (60), Expect = 5.2
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = -1
Query: 345 LTPRKFSSAITPSLAAHWNPATTESLISLRSEL 247
+TP FS P A W +T++ LI++RS L
Sbjct: 1 MTPAIFSPTTLPPSTATWPCSTSQKLITVRSPL 33
>At5g15580.1 68418.m01824 expressed protein unknown protein F14P3.18
- Arabidopsis thaliana, EMBL:AC009755
Length = 927
Score = 28.3 bits (60), Expect = 5.2
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +2
Query: 107 RESPR*LQDSRSVSYRKVRIRCYRSP*DLV 196
+E+PR DSRS S+R R C P +LV
Sbjct: 223 KETPRLSLDSRSNSFRSARSSCSPEPQELV 252
>At5g26210.1 68418.m03119 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 255
Score = 27.9 bits (59), Expect = 6.9
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +3
Query: 6 DQLCLSKSPNKHNRLFMKAQPMPDGLPEDI 95
+ LCL PN+H + + A+ +P LPE +
Sbjct: 46 ENLCLYGHPNEHWEVNLPAEEVPPELPEPV 75
>At2g41770.1 68415.m05163 expressed protein contains Pfam domain
PF03385: Protein of unknown function, DUF288
Length = 771
Score = 27.9 bits (59), Expect = 6.9
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = -2
Query: 284 PQQSP*FH*GLNSFGAIHQDVGAGTLGAKAPNLTGFGNIVFVLFCKIP 141
P++ P H G+ G + ++G K FGN+V V+FC P
Sbjct: 487 PRKLPSVHLGVEETGTVSTEIGNLIRWRK-----NFGNVVLVMFCNGP 529
>At5g26920.1 68418.m03210 calmodulin-binding protein similar to
calmodulin-binding protein TCB60 GI:1698548 from
[Nicotiana tabacum]
Length = 492
Score = 27.5 bits (58), Expect = 9.2
Identities = 20/82 (24%), Positives = 34/82 (41%), Gaps = 2/82 (2%)
Frame = -3
Query: 559 LRKRDHDVCSAHRKYHPSYFRTLNFTKIYRLHETGLSS*QTCVQASSSCWNDLATTSMDG 380
L K D SA R +F ++Y ++ L + S WN + + +MD
Sbjct: 175 LEKIAKDGVSATRLAERKILTVKDFRRLYTVNRNELHN-IIGAGVSKKTWNTIVSHAMDC 233
Query: 379 I--SMECYIIDVESNTTQILFS 320
+ ECYI + + +LF+
Sbjct: 234 VLDETECYIYNANTPGVTLLFN 255
>At3g63180.1 68416.m07097 expressed protein
Length = 978
Score = 27.5 bits (58), Expect = 9.2
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +2
Query: 599 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSS 703
P ++ + L P+ VP+ + RP++S + +G +S
Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLAS 573
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,137,763
Number of Sequences: 28952
Number of extensions: 387441
Number of successful extensions: 1032
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 996
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1031
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -