BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00200
(753 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 27 2.2
SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schiz... 27 2.9
SPBC1271.06c |mug96||sequence orphan|Schizosaccharomyces pombe|c... 27 3.8
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 26 5.0
SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccha... 26 6.6
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 25 8.8
SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc... 25 8.8
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 27.5 bits (58), Expect = 2.2
Identities = 10/29 (34%), Positives = 20/29 (68%)
Frame = +1
Query: 388 PNELYRRLLPAMLFVLTFVTVMTMLLIYM 474
P+ Y+RL+ +LF T++T+LL+++
Sbjct: 954 PSNFYKRLIALLLFCWICSTLVTVLLVFV 982
>SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase
Ssp2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 576
Score = 27.1 bits (57), Expect = 2.9
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +3
Query: 564 IAYVRQLHLTARSKPQDVVTTPTEQVKVLDSIFGE 668
I+Y++ L K DV+TTPT+ V V++ GE
Sbjct: 83 ISYLKLLRHPHIIKLYDVITTPTDIVMVIEYAGGE 117
>SPBC1271.06c |mug96||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 146
Score = 26.6 bits (56), Expect = 3.8
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +1
Query: 661 LERNCTMAHLWSG 699
L+ NCT+A LWSG
Sbjct: 126 LQHNCTLADLWSG 138
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 26.2 bits (55), Expect = 5.0
Identities = 9/35 (25%), Positives = 19/35 (54%)
Frame = -2
Query: 698 PLHKCAIVQFLSKNGIQNLHLLCRCGNNILRFGTS 594
P +++ L + G + +C +NI+RFG++
Sbjct: 277 PTEVASVISTLMEQGFWSFEAICIYSDNIIRFGSN 311
>SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 25.8 bits (54), Expect = 6.6
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 4/62 (6%)
Frame = +3
Query: 516 TRDYELARIGQGSAALIAYVRQLHLTARS-KPQDVVTTPTEQVKVLDSIFGEELYN---G 683
T + L +I G + ++Y+ + R KP +++ T QVK+ D EL N G
Sbjct: 178 TGERPLGKIAFGVLSGLSYLHDRKIIHRDIKPSNILLTSKGQVKLCDFGVSGELVNSLAG 237
Query: 684 TF 689
TF
Sbjct: 238 TF 239
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 25.4 bits (53), Expect = 8.8
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +1
Query: 283 PPEPKPNLIDKLVIIFAETKTHSPRFTLPAFMKAPPNELYRR 408
PP+ L+DKLV F E++ +P F + P Y R
Sbjct: 440 PPQTCSRLLDKLVGEFIESECINPTFIIGHPQMMSPLAKYHR 481
>SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 489
Score = 25.4 bits (53), Expect = 8.8
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +2
Query: 152 ISGLCPFFVFFLMQGIENKVIMAEKTDQDNPPP 250
I L P FVF I+ +V+ ++ +N PP
Sbjct: 438 IQNLPPLFVFNARSVIDKEVVPEQEKSAENQPP 470
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,130,749
Number of Sequences: 5004
Number of extensions: 65728
Number of successful extensions: 165
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -