BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00200 (753 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 27 2.2 SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schiz... 27 2.9 SPBC1271.06c |mug96||sequence orphan|Schizosaccharomyces pombe|c... 27 3.8 SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 26 5.0 SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccha... 26 6.6 SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 25 8.8 SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc... 25 8.8 >SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1242 Score = 27.5 bits (58), Expect = 2.2 Identities = 10/29 (34%), Positives = 20/29 (68%) Frame = +1 Query: 388 PNELYRRLLPAMLFVLTFVTVMTMLLIYM 474 P+ Y+RL+ +LF T++T+LL+++ Sbjct: 954 PSNFYKRLIALLLFCWICSTLVTVLLVFV 982 >SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schizosaccharomyces pombe|chr 3|||Manual Length = 576 Score = 27.1 bits (57), Expect = 2.9 Identities = 14/35 (40%), Positives = 21/35 (60%) Frame = +3 Query: 564 IAYVRQLHLTARSKPQDVVTTPTEQVKVLDSIFGE 668 I+Y++ L K DV+TTPT+ V V++ GE Sbjct: 83 ISYLKLLRHPHIIKLYDVITTPTDIVMVIEYAGGE 117 >SPBC1271.06c |mug96||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 146 Score = 26.6 bits (56), Expect = 3.8 Identities = 9/13 (69%), Positives = 11/13 (84%) Frame = +1 Query: 661 LERNCTMAHLWSG 699 L+ NCT+A LWSG Sbjct: 126 LQHNCTLADLWSG 138 >SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1133 Score = 26.2 bits (55), Expect = 5.0 Identities = 9/35 (25%), Positives = 19/35 (54%) Frame = -2 Query: 698 PLHKCAIVQFLSKNGIQNLHLLCRCGNNILRFGTS 594 P +++ L + G + +C +NI+RFG++ Sbjct: 277 PTEVASVISTLMEQGFWSFEAICIYSDNIIRFGSN 311 >SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 25.8 bits (54), Expect = 6.6 Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 4/62 (6%) Frame = +3 Query: 516 TRDYELARIGQGSAALIAYVRQLHLTARS-KPQDVVTTPTEQVKVLDSIFGEELYN---G 683 T + L +I G + ++Y+ + R KP +++ T QVK+ D EL N G Sbjct: 178 TGERPLGKIAFGVLSGLSYLHDRKIIHRDIKPSNILLTSKGQVKLCDFGVSGELVNSLAG 237 Query: 684 TF 689 TF Sbjct: 238 TF 239 >SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 591 Score = 25.4 bits (53), Expect = 8.8 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +1 Query: 283 PPEPKPNLIDKLVIIFAETKTHSPRFTLPAFMKAPPNELYRR 408 PP+ L+DKLV F E++ +P F + P Y R Sbjct: 440 PPQTCSRLLDKLVGEFIESECINPTFIIGHPQMMSPLAKYHR 481 >SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 489 Score = 25.4 bits (53), Expect = 8.8 Identities = 11/33 (33%), Positives = 17/33 (51%) Frame = +2 Query: 152 ISGLCPFFVFFLMQGIENKVIMAEKTDQDNPPP 250 I L P FVF I+ +V+ ++ +N PP Sbjct: 438 IQNLPPLFVFNARSVIDKEVVPEQEKSAENQPP 470 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,130,749 Number of Sequences: 5004 Number of extensions: 65728 Number of successful extensions: 165 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 163 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 165 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 359287726 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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