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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00189
         (543 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha...   141   7e-35
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch...    94   1e-20
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch...    87   1e-18
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac...    83   3e-17
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac...    78   7e-16
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2...    73   2e-14
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    73   4e-14
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac...    70   3e-13
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...    70   3e-13
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch...    62   7e-11
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac...    55   6e-09
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo...    54   1e-08
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    44   1e-05
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos...    33   0.027
SPCPB1C11.03 |||cysteine transporter |Schizosaccharomyces pombe|...    27   1.4  
SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomy...    25   9.5  

>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
           Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score =  141 bits (341), Expect = 7e-35
 Identities = 67/91 (73%), Positives = 72/91 (79%)
 Frame = +1

Query: 259 SVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKV 438
           S Y GGVFFL IHF  +YPFKPPKV F TRIYH NINS G ICLDIL+D WSPALTISKV
Sbjct: 43  SPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKV 102

Query: 439 LLSRCSLLTDCNPADPLVGSIATHIYRIGKS 531
           LLS CSLLTD NP DPLV  IA H+Y+  +S
Sbjct: 103 LLSICSLLTDPNPDDPLVPEIA-HVYKTDRS 132



 Score = 56.8 bits (131), Expect = 2e-09
 Identities = 22/37 (59%), Positives = 30/37 (81%)
 Frame = +2

Query: 143 KRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGP 253
           KRI +ELA++  DPP +CSAGP GD+L+ W +TI+GP
Sbjct: 4   KRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGP 40


>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
           Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 217

 Score = 94.3 bits (224), Expect = 1e-20
 Identities = 43/84 (51%), Positives = 56/84 (66%), Gaps = 1/84 (1%)
 Frame = +1

Query: 256 GSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQ-GVICLDILKDNWSPALTIS 432
           G+ YEGG F +DI    +YPF+PPK+ F T+IYH N++SQ G ICLDILKD WSP  T+ 
Sbjct: 45  GTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVYTMK 104

Query: 433 KVLLSRCSLLTDCNPADPLVGSIA 504
             L+S  SLL    P++P    +A
Sbjct: 105 SALISLQSLLCTPEPSNPQDAQVA 128


>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
           Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 87.4 bits (207), Expect = 1e-18
 Identities = 42/82 (51%), Positives = 51/82 (62%)
 Frame = +1

Query: 259 SVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKV 438
           S YEGG F L++    EYP  PP V F T+IYH N++  G ICL  LK +WSPAL I  V
Sbjct: 44  SAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICLSTLKKDWSPALQIRTV 103

Query: 439 LLSRCSLLTDCNPADPLVGSIA 504
           LLS  +L+   NP DPL   +A
Sbjct: 104 LLSIQALMGAPNPDDPLDNDVA 125



 Score = 39.5 bits (88), Expect = 3e-04
 Identities = 19/38 (50%), Positives = 23/38 (60%)
 Frame = +2

Query: 143 KRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPQ 256
           KRI KE+  +T DPPP   A P  DNL  +  T+ GPQ
Sbjct: 5   KRIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQ 42


>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
           Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 155

 Score = 82.6 bits (195), Expect = 3e-17
 Identities = 40/83 (48%), Positives = 52/83 (62%), Gaps = 1/83 (1%)
 Frame = +1

Query: 259 SVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDN-WSPALTISK 435
           SVY GG F   + F  +YPF+PP + F TRIYH N +S+G +CL ILK   + P++ +  
Sbjct: 49  SVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIKLRS 108

Query: 436 VLLSRCSLLTDCNPADPLVGSIA 504
           VL     LL + NP DPLV SIA
Sbjct: 109 VLEQILQLLREPNPDDPLVASIA 131



 Score = 37.5 bits (83), Expect = 0.001
 Identities = 16/46 (34%), Positives = 26/46 (56%)
 Frame = +2

Query: 116 MSKALGTSAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGP 253
           M+ A  +S++R+ KE +++   P P+       DNL+ W  T LGP
Sbjct: 1   MASASPSSSRRLTKEYSDLREHPIPDIRVNLVDDNLFHWACTALGP 46


>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
           Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 160

 Score = 78.2 bits (184), Expect = 7e-16
 Identities = 40/95 (42%), Positives = 57/95 (60%), Gaps = 1/95 (1%)
 Frame = +1

Query: 253 TGSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNIN-SQGVICLDILKDNWSPALTI 429
           T + YEGG + LDIH    YP  PP V F+T+I H NI+ + G +C+DILK +WSPA ++
Sbjct: 45  TETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMDILKTHWSPAWSL 104

Query: 430 SKVLLSRCSLLTDCNPADPLVGSIATHIYRIGKST 534
               L+  SLL++ + + PL    A  + R G  T
Sbjct: 105 QSACLAIISLLSNYDASSPL-NVDAAKLLRTGDKT 138


>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 176

 Score = 73.3 bits (172), Expect = 2e-14
 Identities = 33/75 (44%), Positives = 45/75 (60%)
 Frame = +1

Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLL 444
           YEG  F + + F   YP+ PP + F + ++H N++  G ICLDILKD WS    +  +LL
Sbjct: 73  YEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNICLDILKDKWSAVYNVQTILL 132

Query: 445 SRCSLLTDCNPADPL 489
           S  SLL + N A PL
Sbjct: 133 SLQSLLGEPNNASPL 147


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 72.5 bits (170), Expect = 4e-14
 Identities = 35/88 (39%), Positives = 52/88 (59%)
 Frame = +1

Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLL 444
           +E G F L + F  +YP KPP V F + ++H N+ + G +CLDIL++ WSP   ++ +L 
Sbjct: 48  FEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDILQNRWSPTYDVAAILT 107

Query: 445 SRCSLLTDCNPADPLVGSIATHIYRIGK 528
           S  SLL D N A P   + A  ++R  K
Sbjct: 108 SIQSLLNDPNNASP-ANAEAAQLHRENK 134



 Score = 37.5 bits (83), Expect = 0.001
 Identities = 14/40 (35%), Positives = 23/40 (57%)
 Frame = +2

Query: 134 TSAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGP 253
           T+ +R+ ++   +  DPP   SA P  DN+  W + I+GP
Sbjct: 4   TARRRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGP 43


>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
           Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 177

 Score = 69.7 bits (163), Expect = 3e-13
 Identities = 31/85 (36%), Positives = 47/85 (55%)
 Frame = +1

Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLL 444
           Y+GG F   I     YP  PPKV    +IYH NI+ +G +CL+IL+ +W+P L ++ +L+
Sbjct: 65  YKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWNPVLNLNSILV 124

Query: 445 SRCSLLTDCNPADPLVGSIATHIYR 519
               L    N  DPL    A  +++
Sbjct: 125 GLQFLFLSPNAEDPLNKEAAADLHK 149


>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score = 69.7 bits (163), Expect = 3e-13
 Identities = 32/76 (42%), Positives = 46/76 (60%), Gaps = 2/76 (2%)
 Frame = +1

Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDIL--KDNWSPALTISKV 438
           +EGG++ L + F  EYP +PPK  F   ++H N+   G +CL IL  ++ W PA+TI ++
Sbjct: 53  WEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSILNEEEGWKPAITIKQI 112

Query: 439 LLSRCSLLTDCNPADP 486
           LL    LL D N A P
Sbjct: 113 LLGIQDLLDDPNIASP 128


>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
           Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score = 61.7 bits (143), Expect = 7e-11
 Identities = 33/89 (37%), Positives = 49/89 (55%), Gaps = 4/89 (4%)
 Frame = +1

Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINS-QGVICLDILKDNWSP---ALTIS 432
           Y GG++ + +    EYP+K P + F  RI+H NI+   G +CLD++   WSP    + I 
Sbjct: 44  YSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFDMINIF 103

Query: 433 KVLLSRCSLLTDCNPADPLVGSIATHIYR 519
           +V L +  LL   N +DPL G  A  + R
Sbjct: 104 EVFLPQ--LLRYPNASDPLNGEAAALLLR 130


>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
           Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 167

 Score = 55.2 bits (127), Expect = 6e-09
 Identities = 28/89 (31%), Positives = 44/89 (49%), Gaps = 13/89 (14%)
 Frame = +1

Query: 259 SVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILK------------ 402
           ++YEGG F   + F  +YP  PPK+ F T I+H N++  G +C+ IL             
Sbjct: 48  TLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDA 107

Query: 403 -DNWSPALTISKVLLSRCSLLTDCNPADP 486
            + W P  +   +L+S  S+L+  N   P
Sbjct: 108 GERWLPVHSPETILISVISMLSSPNDESP 136


>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
           Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 54.4 bits (125), Expect = 1e-08
 Identities = 26/86 (30%), Positives = 43/86 (50%), Gaps = 13/86 (15%)
 Frame = +1

Query: 256 GSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDIL------------ 399
           G+ +EGG++   + F  +YP  PP + F    +H N+   G +C+ IL            
Sbjct: 47  GTPFEGGLYPATLKFPSDYPLGPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMYES 106

Query: 400 -KDNWSPALTISKVLLSRCSLLTDCN 474
             + WSP  ++ K+LLS  S+L + N
Sbjct: 107 SSERWSPVQSVEKILLSVMSMLAEPN 132



 Score = 33.9 bits (74), Expect = 0.016
 Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
 Frame = +2

Query: 116 MSKALGTSAKRIQKELAEITLDPPPNCSAGPKG-DNLYEWVSTILGP 253
           MSKA+  + +R+ KE  E+T + P   +AGP   D+ + W   I GP
Sbjct: 1   MSKAM--ALRRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGP 45


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 23/70 (32%), Positives = 37/70 (52%), Gaps = 3/70 (4%)
 Frame = +1

Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICL---DILKDNWSPALTISK 435
           YEGG +   + F P+YPFKPP +   T       N++  +CL   D    +W+P+  +S 
Sbjct: 49  YEGGQYHGTLIFPPDYPFKPPAIRMITPSGRFQTNTR--LCLSFSDFHPKSWNPSWMVST 106

Query: 436 VLLSRCSLLT 465
           +L+   S +T
Sbjct: 107 ILVGLVSFMT 116



 Score = 28.3 bits (60), Expect = 0.77
 Identities = 13/37 (35%), Positives = 18/37 (48%)
 Frame = +2

Query: 143 KRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGP 253
           KR+ KE   +  +P     A P  +N+ EW   I GP
Sbjct: 8   KRLMKEYLALQKNPVELVDAKPATENILEWHYIITGP 44


>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
           Mms2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 139

 Score = 33.1 bits (72), Expect = 0.027
 Identities = 15/31 (48%), Positives = 19/31 (61%)
 Frame = +1

Query: 259 SVYEGGVFFLDIHFSPEYPFKPPKVTFRTRI 351
           SV+E  ++ L IH    YP  PP VTF +RI
Sbjct: 51  SVHENRIYSLKIHCDANYPDAPPIVTFVSRI 81


>SPCPB1C11.03 |||cysteine transporter |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 570

 Score = 27.5 bits (58), Expect = 1.4
 Identities = 14/35 (40%), Positives = 16/35 (45%), Gaps = 1/35 (2%)
 Frame = +1

Query: 244 PWATGSVYEGGV-FFLDIHFSPEYPFKPPKVTFRT 345
           PW    +  GG+ FFL I     YP  P K  F T
Sbjct: 258 PWKLFMIITGGITFFLSIFLFFYYPDNPSKARFLT 292


>SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 327

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = -2

Query: 368 LMLQ*YILVRNVTFGGLNGYSGEK*MSR 285
           L+ Q YILV+  T   + GY G K + R
Sbjct: 171 LLTQCYILVQGTTVAVMGGYKGLKEVRR 198


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,559,721
Number of Sequences: 5004
Number of extensions: 57900
Number of successful extensions: 173
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 167
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 223909422
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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