BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00189 (543 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 141 7e-35 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 94 1e-20 SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 87 1e-18 SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 83 3e-17 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 78 7e-16 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 73 2e-14 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 73 4e-14 SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 70 3e-13 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 70 3e-13 SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 62 7e-11 SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 55 6e-09 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 54 1e-08 SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 44 1e-05 SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 33 0.027 SPCPB1C11.03 |||cysteine transporter |Schizosaccharomyces pombe|... 27 1.4 SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomy... 25 9.5 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 141 bits (341), Expect = 7e-35 Identities = 67/91 (73%), Positives = 72/91 (79%) Frame = +1 Query: 259 SVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKV 438 S Y GGVFFL IHF +YPFKPPKV F TRIYH NINS G ICLDIL+D WSPALTISKV Sbjct: 43 SPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKV 102 Query: 439 LLSRCSLLTDCNPADPLVGSIATHIYRIGKS 531 LLS CSLLTD NP DPLV IA H+Y+ +S Sbjct: 103 LLSICSLLTDPNPDDPLVPEIA-HVYKTDRS 132 Score = 56.8 bits (131), Expect = 2e-09 Identities = 22/37 (59%), Positives = 30/37 (81%) Frame = +2 Query: 143 KRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGP 253 KRI +ELA++ DPP +CSAGP GD+L+ W +TI+GP Sbjct: 4 KRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGP 40 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 94.3 bits (224), Expect = 1e-20 Identities = 43/84 (51%), Positives = 56/84 (66%), Gaps = 1/84 (1%) Frame = +1 Query: 256 GSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQ-GVICLDILKDNWSPALTIS 432 G+ YEGG F +DI +YPF+PPK+ F T+IYH N++SQ G ICLDILKD WSP T+ Sbjct: 45 GTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVYTMK 104 Query: 433 KVLLSRCSLLTDCNPADPLVGSIA 504 L+S SLL P++P +A Sbjct: 105 SALISLQSLLCTPEPSNPQDAQVA 128 >SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Schizosaccharomyces pombe|chr 1|||Manual Length = 148 Score = 87.4 bits (207), Expect = 1e-18 Identities = 42/82 (51%), Positives = 51/82 (62%) Frame = +1 Query: 259 SVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKV 438 S YEGG F L++ EYP PP V F T+IYH N++ G ICL LK +WSPAL I V Sbjct: 44 SAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICLSTLKKDWSPALQIRTV 103 Query: 439 LLSRCSLLTDCNPADPLVGSIA 504 LLS +L+ NP DPL +A Sbjct: 104 LLSIQALMGAPNPDDPLDNDVA 125 Score = 39.5 bits (88), Expect = 3e-04 Identities = 19/38 (50%), Positives = 23/38 (60%) Frame = +2 Query: 143 KRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPQ 256 KRI KE+ +T DPPP A P DNL + T+ GPQ Sbjct: 5 KRIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQ 42 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 82.6 bits (195), Expect = 3e-17 Identities = 40/83 (48%), Positives = 52/83 (62%), Gaps = 1/83 (1%) Frame = +1 Query: 259 SVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDN-WSPALTISK 435 SVY GG F + F +YPF+PP + F TRIYH N +S+G +CL ILK + P++ + Sbjct: 49 SVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIKLRS 108 Query: 436 VLLSRCSLLTDCNPADPLVGSIA 504 VL LL + NP DPLV SIA Sbjct: 109 VLEQILQLLREPNPDDPLVASIA 131 Score = 37.5 bits (83), Expect = 0.001 Identities = 16/46 (34%), Positives = 26/46 (56%) Frame = +2 Query: 116 MSKALGTSAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGP 253 M+ A +S++R+ KE +++ P P+ DNL+ W T LGP Sbjct: 1 MASASPSSSRRLTKEYSDLREHPIPDIRVNLVDDNLFHWACTALGP 46 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 78.2 bits (184), Expect = 7e-16 Identities = 40/95 (42%), Positives = 57/95 (60%), Gaps = 1/95 (1%) Frame = +1 Query: 253 TGSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNIN-SQGVICLDILKDNWSPALTI 429 T + YEGG + LDIH YP PP V F+T+I H NI+ + G +C+DILK +WSPA ++ Sbjct: 45 TETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMDILKTHWSPAWSL 104 Query: 430 SKVLLSRCSLLTDCNPADPLVGSIATHIYRIGKST 534 L+ SLL++ + + PL A + R G T Sbjct: 105 QSACLAIISLLSNYDASSPL-NVDAAKLLRTGDKT 138 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 73.3 bits (172), Expect = 2e-14 Identities = 33/75 (44%), Positives = 45/75 (60%) Frame = +1 Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLL 444 YEG F + + F YP+ PP + F + ++H N++ G ICLDILKD WS + +LL Sbjct: 73 YEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNICLDILKDKWSAVYNVQTILL 132 Query: 445 SRCSLLTDCNPADPL 489 S SLL + N A PL Sbjct: 133 SLQSLLGEPNNASPL 147 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 72.5 bits (170), Expect = 4e-14 Identities = 35/88 (39%), Positives = 52/88 (59%) Frame = +1 Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLL 444 +E G F L + F +YP KPP V F + ++H N+ + G +CLDIL++ WSP ++ +L Sbjct: 48 FEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDILQNRWSPTYDVAAILT 107 Query: 445 SRCSLLTDCNPADPLVGSIATHIYRIGK 528 S SLL D N A P + A ++R K Sbjct: 108 SIQSLLNDPNNASP-ANAEAAQLHRENK 134 Score = 37.5 bits (83), Expect = 0.001 Identities = 14/40 (35%), Positives = 23/40 (57%) Frame = +2 Query: 134 TSAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGP 253 T+ +R+ ++ + DPP SA P DN+ W + I+GP Sbjct: 4 TARRRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGP 43 >SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 69.7 bits (163), Expect = 3e-13 Identities = 31/85 (36%), Positives = 47/85 (55%) Frame = +1 Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLL 444 Y+GG F I YP PPKV +IYH NI+ +G +CL+IL+ +W+P L ++ +L+ Sbjct: 65 YKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWNPVLNLNSILV 124 Query: 445 SRCSLLTDCNPADPLVGSIATHIYR 519 L N DPL A +++ Sbjct: 125 GLQFLFLSPNAEDPLNKEAAADLHK 149 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 69.7 bits (163), Expect = 3e-13 Identities = 32/76 (42%), Positives = 46/76 (60%), Gaps = 2/76 (2%) Frame = +1 Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDIL--KDNWSPALTISKV 438 +EGG++ L + F EYP +PPK F ++H N+ G +CL IL ++ W PA+TI ++ Sbjct: 53 WEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSILNEEEGWKPAITIKQI 112 Query: 439 LLSRCSLLTDCNPADP 486 LL LL D N A P Sbjct: 113 LLGIQDLLDDPNIASP 128 >SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 61.7 bits (143), Expect = 7e-11 Identities = 33/89 (37%), Positives = 49/89 (55%), Gaps = 4/89 (4%) Frame = +1 Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINS-QGVICLDILKDNWSP---ALTIS 432 Y GG++ + + EYP+K P + F RI+H NI+ G +CLD++ WSP + I Sbjct: 44 YSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFDMINIF 103 Query: 433 KVLLSRCSLLTDCNPADPLVGSIATHIYR 519 +V L + LL N +DPL G A + R Sbjct: 104 EVFLPQ--LLRYPNASDPLNGEAAALLLR 130 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 55.2 bits (127), Expect = 6e-09 Identities = 28/89 (31%), Positives = 44/89 (49%), Gaps = 13/89 (14%) Frame = +1 Query: 259 SVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILK------------ 402 ++YEGG F + F +YP PPK+ F T I+H N++ G +C+ IL Sbjct: 48 TLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDA 107 Query: 403 -DNWSPALTISKVLLSRCSLLTDCNPADP 486 + W P + +L+S S+L+ N P Sbjct: 108 GERWLPVHSPETILISVISMLSSPNDESP 136 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 54.4 bits (125), Expect = 1e-08 Identities = 26/86 (30%), Positives = 43/86 (50%), Gaps = 13/86 (15%) Frame = +1 Query: 256 GSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDIL------------ 399 G+ +EGG++ + F +YP PP + F +H N+ G +C+ IL Sbjct: 47 GTPFEGGLYPATLKFPSDYPLGPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMYES 106 Query: 400 -KDNWSPALTISKVLLSRCSLLTDCN 474 + WSP ++ K+LLS S+L + N Sbjct: 107 SSERWSPVQSVEKILLSVMSMLAEPN 132 Score = 33.9 bits (74), Expect = 0.016 Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 1/47 (2%) Frame = +2 Query: 116 MSKALGTSAKRIQKELAEITLDPPPNCSAGPKG-DNLYEWVSTILGP 253 MSKA+ + +R+ KE E+T + P +AGP D+ + W I GP Sbjct: 1 MSKAM--ALRRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGP 45 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 44.4 bits (100), Expect = 1e-05 Identities = 23/70 (32%), Positives = 37/70 (52%), Gaps = 3/70 (4%) Frame = +1 Query: 265 YEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICL---DILKDNWSPALTISK 435 YEGG + + F P+YPFKPP + T N++ +CL D +W+P+ +S Sbjct: 49 YEGGQYHGTLIFPPDYPFKPPAIRMITPSGRFQTNTR--LCLSFSDFHPKSWNPSWMVST 106 Query: 436 VLLSRCSLLT 465 +L+ S +T Sbjct: 107 ILVGLVSFMT 116 Score = 28.3 bits (60), Expect = 0.77 Identities = 13/37 (35%), Positives = 18/37 (48%) Frame = +2 Query: 143 KRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGP 253 KR+ KE + +P A P +N+ EW I GP Sbjct: 8 KRLMKEYLALQKNPVELVDAKPATENILEWHYIITGP 44 >SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizosaccharomyces pombe|chr 3|||Manual Length = 139 Score = 33.1 bits (72), Expect = 0.027 Identities = 15/31 (48%), Positives = 19/31 (61%) Frame = +1 Query: 259 SVYEGGVFFLDIHFSPEYPFKPPKVTFRTRI 351 SV+E ++ L IH YP PP VTF +RI Sbjct: 51 SVHENRIYSLKIHCDANYPDAPPIVTFVSRI 81 >SPCPB1C11.03 |||cysteine transporter |Schizosaccharomyces pombe|chr 3|||Manual Length = 570 Score = 27.5 bits (58), Expect = 1.4 Identities = 14/35 (40%), Positives = 16/35 (45%), Gaps = 1/35 (2%) Frame = +1 Query: 244 PWATGSVYEGGV-FFLDIHFSPEYPFKPPKVTFRT 345 PW + GG+ FFL I YP P K F T Sbjct: 258 PWKLFMIITGGITFFLSIFLFFYYPDNPSKARFLT 292 >SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomyces pombe|chr 2|||Manual Length = 327 Score = 24.6 bits (51), Expect = 9.5 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -2 Query: 368 LMLQ*YILVRNVTFGGLNGYSGEK*MSR 285 L+ Q YILV+ T + GY G K + R Sbjct: 171 LLTQCYILVQGTTVAVMGGYKGLKEVRR 198 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,559,721 Number of Sequences: 5004 Number of extensions: 57900 Number of successful extensions: 173 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 160 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 167 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 223909422 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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