BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00186
(659 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC14F5.05c |sam1||S-adenosylmethionine synthetase |Schizosacch... 116 4e-27
SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|ch... 25 7.3
>SPBC14F5.05c |sam1||S-adenosylmethionine synthetase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 382
Score = 116 bits (278), Expect = 4e-27
Identities = 54/87 (62%), Positives = 62/87 (71%)
Frame = +3
Query: 3 KVIKEVIPAQYLDERTVIHINPCGLFIIGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXX 182
K+IK +PA LDE+TV HI P G F++GGPQ DAGLTGRKIIVDTY
Sbjct: 212 KIIKPTVPAHLLDEKTVYHIQPSGRFVVGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSG 271
Query: 183 KDFTKVDRSAAYAARWVAKSLVKAACA 263
KD++KVDRSAAYAARW+AKSLV A A
Sbjct: 272 KDYSKVDRSAAYAARWIAKSLVAAGLA 298
Score = 115 bits (276), Expect = 7e-27
Identities = 52/85 (61%), Positives = 63/85 (74%)
Frame = +2
Query: 254 GLCRRCMVQVAYAIGVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRA 433
GL RRC+VQ++YAIGVAEPLSI V YGTS KT +L+ I++KNFDLRPG +VK L L+
Sbjct: 296 GLARRCLVQLSYAIGVAEPLSIFVNTYGTSSKTSAELVEIIRKNFDLRPGVLVKSLKLQT 355
Query: 434 PIYQRTSTYGHFGRAGFPWESPKPL 508
P Y T++YGHF PWE PK L
Sbjct: 356 PFYLSTASYGHFTDQSKPWEQPKEL 380
>SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|chr
3|||Manual
Length = 752
Score = 25.4 bits (53), Expect = 7.3
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +1
Query: 352 SATTALHRTKEFRFTTGQNRQRVEPQSTNLSENKYLWTLWKG 477
SA A + F G++ ++P S++L KY+ TL KG
Sbjct: 255 SAIQAFESILQNTFFNGKSNPTIDPNSSSLLSEKYI-TLDKG 295
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,750,514
Number of Sequences: 5004
Number of extensions: 56003
Number of successful extensions: 126
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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