BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00186 (659 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC14F5.05c |sam1||S-adenosylmethionine synthetase |Schizosacch... 116 4e-27 SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|ch... 25 7.3 >SPBC14F5.05c |sam1||S-adenosylmethionine synthetase |Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 116 bits (278), Expect = 4e-27 Identities = 54/87 (62%), Positives = 62/87 (71%) Frame = +3 Query: 3 KVIKEVIPAQYLDERTVIHINPCGLFIIGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXX 182 K+IK +PA LDE+TV HI P G F++GGPQ DAGLTGRKIIVDTY Sbjct: 212 KIIKPTVPAHLLDEKTVYHIQPSGRFVVGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSG 271 Query: 183 KDFTKVDRSAAYAARWVAKSLVKAACA 263 KD++KVDRSAAYAARW+AKSLV A A Sbjct: 272 KDYSKVDRSAAYAARWIAKSLVAAGLA 298 Score = 115 bits (276), Expect = 7e-27 Identities = 52/85 (61%), Positives = 63/85 (74%) Frame = +2 Query: 254 GLCRRCMVQVAYAIGVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRA 433 GL RRC+VQ++YAIGVAEPLSI V YGTS KT +L+ I++KNFDLRPG +VK L L+ Sbjct: 296 GLARRCLVQLSYAIGVAEPLSIFVNTYGTSSKTSAELVEIIRKNFDLRPGVLVKSLKLQT 355 Query: 434 PIYQRTSTYGHFGRAGFPWESPKPL 508 P Y T++YGHF PWE PK L Sbjct: 356 PFYLSTASYGHFTDQSKPWEQPKEL 380 >SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 752 Score = 25.4 bits (53), Expect = 7.3 Identities = 14/42 (33%), Positives = 22/42 (52%) Frame = +1 Query: 352 SATTALHRTKEFRFTTGQNRQRVEPQSTNLSENKYLWTLWKG 477 SA A + F G++ ++P S++L KY+ TL KG Sbjct: 255 SAIQAFESILQNTFFNGKSNPTIDPNSSSLLSEKYI-TLDKG 295 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,750,514 Number of Sequences: 5004 Number of extensions: 56003 Number of successful extensions: 126 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 124 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 126 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 299817502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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