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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00184
         (729 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac...    36   0.008
SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosacc...    28   1.2  
SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease XPF...    27   2.7  
SPAC1834.11c |sec18||secretory pathway protein Sec18 |Schizosacc...    26   4.8  
SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz...    25   8.4  
SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non ca...    25   8.4  
SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma...    25   8.4  
SPCC569.06 |||S. pombe specific multicopy membrane protein famil...    25   8.4  

>SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase
           Lac1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 384

 Score = 35.5 bits (78), Expect = 0.008
 Identities = 18/68 (26%), Positives = 34/68 (50%), Gaps = 1/68 (1%)
 Frame = +1

Query: 493 RQALHLVKFCENCWRCSFYLYNFSYGMFILWEKT-WLWDIDQCYIGYPHQGVTGDVWWYY 669
           R    L +F E  + C ++    S+G++++ +   W ++ D  +  YPH    G    +Y
Sbjct: 138 RAPAKLRRFEEQAYTCLYFTVMGSWGLYVMKQTPMWFFNTDAFWEEYPHFYHVGSFKAFY 197

Query: 670 MISSAFYW 693
           +I +A YW
Sbjct: 198 LIEAA-YW 204


>SPAC1A6.09c |lag1||sphingosine N-acyltransferase
           Lag1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 13/61 (21%), Positives = 30/61 (49%)
 Frame = +1

Query: 508 LVKFCENCWRCSFYLYNFSYGMFILWEKTWLWDIDQCYIGYPHQGVTGDVWWYYMISSAF 687
           +++FCE  +   +YL  +  G++I     +  + ++ +  YP   ++     YY+I   F
Sbjct: 152 IIRFCEQGYSFFYYLCFWFLGLYIYRSSNYWSNEEKLFEDYPQYYMSPLFKAYYLIQLGF 211

Query: 688 Y 690
           +
Sbjct: 212 W 212


>SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease
           XPF|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 892

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 13/38 (34%), Positives = 18/38 (47%)
 Frame = +1

Query: 463 GKMVAFTSQPRQALHLVKFCENCWRCSFYLYNFSYGMF 576
           G + AF+  P Q L  +    +C RC F  + F Y  F
Sbjct: 152 GFIKAFSDDPEQFLMGINALSHCLRCLFLRHVFIYPRF 189


>SPAC1834.11c |sec18||secretory pathway protein Sec18
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 792

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 15/49 (30%), Positives = 27/49 (55%)
 Frame = +3

Query: 339 NTRPKKAPNNPKLENAYQASSKLKHKQICALANKWTYRRDRWKDGGVYV 485
           N R  K  N P++ N Y   S+   +++ A A +  Y RDR ++ G+++
Sbjct: 329 NAREPKIVNGPEILNKYVGQSEENVRKLFADAER-EY-RDRGEESGLHI 375


>SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 527

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 16/47 (34%), Positives = 22/47 (46%)
 Frame = -1

Query: 546 EAASPTVLAELDQVEGLSWLRRKRHHLSTCLSDKSTCSPVHISVYVS 406
           E   PT+   LD   GLSWL R R   +  L      +P+H +  +S
Sbjct: 395 EDIEPTL--RLDHASGLSWLTR-RSVFTAILESSLIINPLHSTSLLS 438


>SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non
           catalytic subunit Arm1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 811

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 9/18 (50%), Positives = 12/18 (66%)
 Frame = -1

Query: 57  IYHAMKIFKINNDTTDHF 4
           +Y  M I +I NDT DH+
Sbjct: 459 VYDTMSIKQIQNDTLDHY 476


>SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 629

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = +3

Query: 249 SVPLALVLIVLRHVLEKYWFAP 314
           S+P  ++LI L  +L+  WF P
Sbjct: 495 SIPFLVILIELFSILDSLWFHP 516


>SPCC569.06 |||S. pombe specific multicopy membrane protein family
           1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 478

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = +1

Query: 493 RQALHLVKFCENCWRCSFYLYN 558
           R  LHLV+ C  CW  SF++ N
Sbjct: 238 RTKLHLVQVCLFCW-FSFFILN 258


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,318,054
Number of Sequences: 5004
Number of extensions: 75466
Number of successful extensions: 202
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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