BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00184 (729 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac... 36 0.008 SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosacc... 28 1.2 SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease XPF... 27 2.7 SPAC1834.11c |sec18||secretory pathway protein Sec18 |Schizosacc... 26 4.8 SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 25 8.4 SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non ca... 25 8.4 SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma... 25 8.4 SPCC569.06 |||S. pombe specific multicopy membrane protein famil... 25 8.4 >SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac1|Schizosaccharomyces pombe|chr 2|||Manual Length = 384 Score = 35.5 bits (78), Expect = 0.008 Identities = 18/68 (26%), Positives = 34/68 (50%), Gaps = 1/68 (1%) Frame = +1 Query: 493 RQALHLVKFCENCWRCSFYLYNFSYGMFILWEKT-WLWDIDQCYIGYPHQGVTGDVWWYY 669 R L +F E + C ++ S+G++++ + W ++ D + YPH G +Y Sbjct: 138 RAPAKLRRFEEQAYTCLYFTVMGSWGLYVMKQTPMWFFNTDAFWEEYPHFYHVGSFKAFY 197 Query: 670 MISSAFYW 693 +I +A YW Sbjct: 198 LIEAA-YW 204 >SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 28.3 bits (60), Expect = 1.2 Identities = 13/61 (21%), Positives = 30/61 (49%) Frame = +1 Query: 508 LVKFCENCWRCSFYLYNFSYGMFILWEKTWLWDIDQCYIGYPHQGVTGDVWWYYMISSAF 687 +++FCE + +YL + G++I + + ++ + YP ++ YY+I F Sbjct: 152 IIRFCEQGYSFFYYLCFWFLGLYIYRSSNYWSNEEKLFEDYPQYYMSPLFKAYYLIQLGF 211 Query: 688 Y 690 + Sbjct: 212 W 212 >SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease XPF|Schizosaccharomyces pombe|chr 3|||Manual Length = 892 Score = 27.1 bits (57), Expect = 2.7 Identities = 13/38 (34%), Positives = 18/38 (47%) Frame = +1 Query: 463 GKMVAFTSQPRQALHLVKFCENCWRCSFYLYNFSYGMF 576 G + AF+ P Q L + +C RC F + F Y F Sbjct: 152 GFIKAFSDDPEQFLMGINALSHCLRCLFLRHVFIYPRF 189 >SPAC1834.11c |sec18||secretory pathway protein Sec18 |Schizosaccharomyces pombe|chr 1|||Manual Length = 792 Score = 26.2 bits (55), Expect = 4.8 Identities = 15/49 (30%), Positives = 27/49 (55%) Frame = +3 Query: 339 NTRPKKAPNNPKLENAYQASSKLKHKQICALANKWTYRRDRWKDGGVYV 485 N R K N P++ N Y S+ +++ A A + Y RDR ++ G+++ Sbjct: 329 NAREPKIVNGPEILNKYVGQSEENVRKLFADAER-EY-RDRGEESGLHI 375 >SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 25.4 bits (53), Expect = 8.4 Identities = 16/47 (34%), Positives = 22/47 (46%) Frame = -1 Query: 546 EAASPTVLAELDQVEGLSWLRRKRHHLSTCLSDKSTCSPVHISVYVS 406 E PT+ LD GLSWL R R + L +P+H + +S Sbjct: 395 EDIEPTL--RLDHASGLSWLTR-RSVFTAILESSLIINPLHSTSLLS 438 >SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non catalytic subunit Arm1|Schizosaccharomyces pombe|chr 2|||Manual Length = 811 Score = 25.4 bits (53), Expect = 8.4 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = -1 Query: 57 IYHAMKIFKINNDTTDHF 4 +Y M I +I NDT DH+ Sbjct: 459 VYDTMSIKQIQNDTLDHY 476 >SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 629 Score = 25.4 bits (53), Expect = 8.4 Identities = 9/22 (40%), Positives = 14/22 (63%) Frame = +3 Query: 249 SVPLALVLIVLRHVLEKYWFAP 314 S+P ++LI L +L+ WF P Sbjct: 495 SIPFLVILIELFSILDSLWFHP 516 >SPCC569.06 |||S. pombe specific multicopy membrane protein family 1|Schizosaccharomyces pombe|chr 3|||Manual Length = 478 Score = 25.4 bits (53), Expect = 8.4 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = +1 Query: 493 RQALHLVKFCENCWRCSFYLYN 558 R LHLV+ C CW SF++ N Sbjct: 238 RTKLHLVQVCLFCW-FSFFILN 258 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,318,054 Number of Sequences: 5004 Number of extensions: 75466 Number of successful extensions: 202 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 185 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 202 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 343230174 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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