BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00184 (729 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g19260.1 68416.m02443 longevity-assurance (LAG1) family prote... 44 1e-04 At3g25540.1 68416.m03175 longevity-assurance (LAG1) family prote... 38 0.007 At1g13580.1 68414.m01592 longevity-assurance (LAG1) family prote... 36 0.021 At3g26935.1 68416.m03371 zinc finger (DHHC type) family protein ... 31 0.78 At4g39550.1 68417.m05592 kelch repeat-containing F-box family pr... 29 3.2 At1g28420.1 68414.m03494 homeobox transcription factor, putative... 29 4.2 At5g43100.1 68418.m05261 aspartyl protease family protein low si... 28 7.3 At3g43860.1 68416.m04692 glycosyl hydrolase family 9 protein sim... 28 7.3 At2g44140.1 68415.m05490 autophagy 4a (APG4a) identical to autop... 28 7.3 At4g39280.1 68417.m05563 phenylalanyl-tRNA synthetase, putative ... 27 9.6 >At3g19260.1 68416.m02443 longevity-assurance (LAG1) family protein similar to Alternaria stem canker resistance protein (ASC1) [Lycopersicon esculentum] GI:7688742; contains Pfam profile PF03798: Longevity-assurance protein (LAG1) Length = 296 Score = 44.0 bits (99), Expect = 1e-04 Identities = 25/107 (23%), Positives = 50/107 (46%), Gaps = 1/107 (0%) Frame = +1 Query: 412 INRYVHWRTSGLIGETGGKMVAFTSQPRQALHLVKFCENCWRCSFYLYNFSYGMFILWEK 591 ++RYV R + + TG + +A +VK E+ W+ +Y + + +++ + Sbjct: 39 LDRYVFQRIALWLLSTGSAPIKLNDAATRA-KIVKCKESLWKLLYYAACDFFVLQVIYHE 97 Query: 592 TWLWDIDQCYIGYPHQGVTGDVWWYYMISSAFY-WSLTISQFWDVKR 729 W DI + G+P+Q + + YYM FY + + W+ +R Sbjct: 98 PWARDIKLYFHGWPNQELKLSIKLYYMCQCGFYVYGVAALLAWETRR 144 >At3g25540.1 68416.m03175 longevity-assurance (LAG1) family protein similar to Alternaria stem canker resistance protein (ASC1) [Lycopersicon esculentum] GI:7688742; contains Pfam profile PF03798: Longevity-assurance protein (LAG1); supporting cDNA gi|7658238|gb|AF198179.1|AF198179 Length = 310 Score = 37.9 bits (84), Expect = 0.007 Identities = 20/78 (25%), Positives = 39/78 (50%), Gaps = 6/78 (7%) Frame = +1 Query: 514 KFCENCWRCSFYLYNFSYGMFILWEKTWLWDIDQCYIG-----YPHQGVTGDVWWYYMIS 678 KF E+ W+C +YL + + + + W + +IG +P Q + + + YM + Sbjct: 77 KFKESAWKCIYYLSAELLALSVTYNEPWFSNTLYFWIGPGDQIWPDQPMKMKLKFLYMFA 136 Query: 679 SAFY-WSLTISQFWDVKR 729 + FY +S+ FW+ +R Sbjct: 137 AGFYTYSIFALVFWETRR 154 >At1g13580.1 68414.m01592 longevity-assurance (LAG1) family protein similar to Alternaria stem canker resistance protein (ASC1) [Lycopersicon esculentum] GI:7688742; contains Pfam profile PF03798: Longevity-assurance protein (LAG1) Length = 239 Score = 36.3 bits (80), Expect = 0.021 Identities = 19/78 (24%), Positives = 36/78 (46%), Gaps = 6/78 (7%) Frame = +1 Query: 514 KFCENCWRCSFYLYNFSYGMFILWEKTWLWDIDQCYIG-----YPHQGVTGDVWWYYMIS 678 KF E+ W+C +YL + + + + W + ++G +P Q + YM Sbjct: 74 KFKESAWKCVYYLSAEILALSVTYNEPWFMNTKYFWVGPGDQTWPDQQTKLKLKLLYMFV 133 Query: 679 SAFY-WSLTISQFWDVKR 729 + FY +S+ FW+ +R Sbjct: 134 AGFYTYSIFALVFWETRR 151 >At3g26935.1 68416.m03371 zinc finger (DHHC type) family protein contains Pfam profile PF01529: DHHC zinc finger domain Length = 443 Score = 31.1 bits (67), Expect = 0.78 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -2 Query: 437 VRQCTYLFMFQFGRCLICIF*FRIVWCFLR 348 +R + FMF F L+CI+ F W ++R Sbjct: 188 MRNYRFFFMFVFSTTLLCIYVFAFCWVYIR 217 >At4g39550.1 68417.m05592 kelch repeat-containing F-box family protein similar to SKP1 interacting partner 6 [Arabidopsis thaliana] GI:10716957; contains Pfam profiles PF01344: Kelch motif, PF00646: F-box domain Length = 392 Score = 29.1 bits (62), Expect = 3.2 Identities = 15/49 (30%), Positives = 27/49 (55%), Gaps = 1/49 (2%) Frame = +1 Query: 583 WEKTWLWDIDQCYIGYPHQGVTGDVWWYYMISSAFYWSLTISQFW-DVK 726 WE+ WD+D ++ Y + V +++YY F W T+++ W D+K Sbjct: 264 WERM-KWDMDIGWVWYSYCVVENVLYYYY--KGVFKWYDTMARLWRDLK 309 >At1g28420.1 68414.m03494 homeobox transcription factor, putative similar to homeobox transcription factor Hox7 GI:19486 [Lycopersicon peruvianum] Length = 1703 Score = 28.7 bits (61), Expect = 4.2 Identities = 11/22 (50%), Positives = 18/22 (81%) Frame = +2 Query: 434 EQVDLSERQVERWWRLRRSQDK 499 E++DLS+RQ++ W+ RR +DK Sbjct: 72 EKLDLSDRQLQMWFCHRRLKDK 93 >At5g43100.1 68418.m05261 aspartyl protease family protein low similarity to CND41, chloroplast nucleoid DNA binding protein [Nicotiana tabacum] GI:2541876; contains Pfam profile PF00026: Eukaryotic aspartyl protease Length = 631 Score = 27.9 bits (59), Expect = 7.3 Identities = 13/42 (30%), Positives = 17/42 (40%) Frame = +3 Query: 471 GGVYVAAKTSPPPGQVLRXXXXXXXXXXXXXXRYVHTMGENL 596 GG V K SPPPG V + +H G++L Sbjct: 233 GGAMVLGKISPPPGMVFSHSDPFRSPYYNIDLKQMHVAGKSL 274 >At3g43860.1 68416.m04692 glycosyl hydrolase family 9 protein similar to cellulase GI:575404 from [Sambucus nigra]. Length = 486 Score = 27.9 bits (59), Expect = 7.3 Identities = 11/33 (33%), Positives = 16/33 (48%), Gaps = 2/33 (6%) Frame = +1 Query: 538 CSFYLYNFSYGMFILWEKTWLWDI--DQCYIGY 630 C FY N Y ++W TWL+ + Y+ Y Sbjct: 229 CPFYCSNSGYNDELIWAATWLYKATRNHLYLSY 261 >At2g44140.1 68415.m05490 autophagy 4a (APG4a) identical to autophagy 4a [Arabidopsis thaliana] gi|19912143|dbj|BAB88383 Length = 467 Score = 27.9 bits (59), Expect = 7.3 Identities = 10/28 (35%), Positives = 15/28 (53%) Frame = +3 Query: 363 NNPKLENAYQASSKLKHKQICALANKWT 446 ++ + Y+ SS HKQ+C N WT Sbjct: 49 SSSSVSQPYRESSTSGHKQVCTTRNGWT 76 >At4g39280.1 68417.m05563 phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative similar to SP|Q9Y285 Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20) (Phenylalanine- -tRNA ligase alpha chain) (PheRS) {Homo sapiens}; contains Pfam profile PF01409: tRNA synthetases class II core domain (F) Length = 485 Score = 27.5 bits (58), Expect = 9.6 Identities = 15/45 (33%), Positives = 24/45 (53%) Frame = -1 Query: 630 IANVALVDIPQPSFLP*YEHTVREVVQIEAASPTVLAELDQVEGL 496 +++ L + Q F+P ++ V + EA T LAE Q+EGL Sbjct: 320 VSSRMLYALAQKPFVPKKYFSIDRVFRNEAVDRTHLAEFHQIEGL 364 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,654,241 Number of Sequences: 28952 Number of extensions: 403701 Number of successful extensions: 972 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 944 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 972 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1594686376 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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