BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00178 (499 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_12239| Best HMM Match : CPSF_A (HMM E-Value=1.2) 113 8e-26 SB_47303| Best HMM Match : CPSF_A (HMM E-Value=0) 32 0.23 SB_54215| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.53 SB_30175| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 8.6 >SB_12239| Best HMM Match : CPSF_A (HMM E-Value=1.2) Length = 864 Score = 113 bits (272), Expect = 8e-26 Identities = 50/66 (75%), Positives = 59/66 (89%) Frame = +1 Query: 256 PGSPNAVWTVRRNKDEEYDSYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCHAMG 435 PG+PNAVWTV+ +E+D+YI+VSF+NATLVLSIGETVEEVTDSGFLGTTPTLSC +G Sbjct: 400 PGNPNAVWTVKHTTADEFDAYIVVSFINATLVLSIGETVEEVTDSGFLGTTPTLSCSQLG 459 Query: 436 NDALVQ 453 +ALVQ Sbjct: 460 EEALVQ 465 Score = 75.8 bits (178), Expect = 2e-14 Identities = 34/54 (62%), Positives = 43/54 (79%) Frame = +2 Query: 2 DDEPEFSSAMPLEEGDTFFFAPRPLRNLVLVDEMDSLSPILACHVADLAGEDTP 163 DDE EFSS M LEEG TFFF PR L+NLVLVDE++SL+PI+ C V+++A + P Sbjct: 347 DDELEFSSTMELEEGTTFFFQPRGLKNLVLVDELESLAPIMNCQVSEMAVSELP 400 Score = 35.9 bits (79), Expect = 0.019 Identities = 14/15 (93%), Positives = 15/15 (100%) Frame = +3 Query: 453 VYPDGIRHIRADKRV 497 +YPDGIRHIRADKRV Sbjct: 466 IYPDGIRHIRADKRV 480 >SB_47303| Best HMM Match : CPSF_A (HMM E-Value=0) Length = 1291 Score = 32.3 bits (70), Expect = 0.23 Identities = 15/66 (22%), Positives = 36/66 (54%) Frame = +1 Query: 298 DEEYDSYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCHAMGNDALVQYILMV*DI 477 +E+Y S++I+S +++++L + + EV SGF T+ GN + + + + ++ Sbjct: 180 NEKYHSFLILSREDSSMILKTEQEIMEVDQSGFSTQCATIYAGNFGNGSYILQLPCISEV 239 Query: 478 FVLTNV 495 L ++ Sbjct: 240 NQLQHI 245 >SB_54215| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 695 Score = 31.1 bits (67), Expect = 0.53 Identities = 18/70 (25%), Positives = 34/70 (48%) Frame = +2 Query: 89 LVDEMDSLSPILACHVADLAGEDTPQVYLACGKGPRSSLRALRHGLEVAEKAVSELQDHR 268 +++ +L PI+ V DL + Q+ G SLR +R+G+ + E A +L Sbjct: 289 VMETFTNLGPIVDMVVVDLERQGQGQLVTCSGAKKEGSLRIIRNGIGIHEHATIDLAGIM 348 Query: 269 MLFGQFDEIK 298 +L +E++ Sbjct: 349 VLSLSGEEVE 358 >SB_30175| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 617 Score = 27.1 bits (57), Expect = 8.6 Identities = 15/69 (21%), Positives = 31/69 (44%) Frame = +2 Query: 107 SLSPILACHVADLAGEDTPQVYLACGKGPRSSLRALRHGLEVAEKAVSELQDHRMLFGQF 286 S P+ H + ++ P + A G+G L L++ + ++ +LQ + L Sbjct: 239 STGPLPPIHPSQHRPQEHPHLPAAAGRGATVKLTGLQNRQQQLQQIQEQLQRRKQLQKMQ 298 Query: 287 DEIKMRNTI 313 +E+K R + Sbjct: 299 EELKHRKLL 307 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,624,636 Number of Sequences: 59808 Number of extensions: 341471 Number of successful extensions: 787 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 760 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 787 length of database: 16,821,457 effective HSP length: 77 effective length of database: 12,216,241 effective search space used: 1075029208 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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