Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00178
(499 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 99 2e-21
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 99 2e-21
At3g11960.2 68416.m01476 cleavage and polyadenylation specificit... 50 1e-06
At3g11960.1 68416.m01475 cleavage and polyadenylation specificit... 50 1e-06
At4g21100.1 68417.m03051 UV-damaged DNA-binding protein, putativ... 48 3e-06
At4g05420.1 68417.m00824 UV-damaged DNA-binding protein, putativ... 48 5e-06
At5g51660.1 68418.m06405 cleavage and polyadenylation specificit... 31 0.57
At4g29490.1 68417.m04208 Xaa-Pro dipeptidase, putative / prolida... 30 1.00
At5g28480.1 68418.m03462 hypothetical protein 29 1.3
At2g12100.1 68415.m01300 Ulp1 protease family protein contains P... 29 1.3
At1g45090.1 68414.m05169 Ulp1 protease family protein similar to... 29 1.3
At1g62970.1 68414.m07110 DNAJ heat shock N-terminal domain-conta... 29 1.7
At1g03670.1 68414.m00346 ankyrin repeat family protein contains ... 29 2.3
At5g65440.1 68418.m08230 expressed protein 27 9.3
At5g43440.1 68418.m05311 2-oxoglutarate-dependent dioxygenase, p... 27 9.3
>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 98.7 bits (235), Expect = 2e-21
Identities = 43/66 (65%), Positives = 56/66 (84%)
Frame = +1
Query: 256 PGSPNAVWTVRRNKDEEYDSYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCHAMG 435
PG P+AVWTV++N +E+D+YI+VSF NATLVLSIGE VEEV DSGFL TTP+L+ +G
Sbjct: 451 PGQPSAVWTVKKNVSDEFDAYIVVSFTNATLVLSIGEQVEEVNDSGFLDTTPSLAVSLIG 510
Query: 436 NDALVQ 453
+D+L+Q
Sbjct: 511 DDSLMQ 516
Score = 85.8 bits (203), Expect = 1e-17
Identities = 42/86 (48%), Positives = 58/86 (67%), Gaps = 1/86 (1%)
Frame = +2
Query: 2 DDEPEFSSAMPLEEG-DTFFFAPRPLRNLVLVDEMDSLSPILACHVADLAGEDTPQVYLA 178
D E S+ M EEG FF PR L+NLV +D+++SL P++ V ++ E+TPQ++
Sbjct: 365 DVESSSSNLMETEEGFQPVFFQPRRLKNLVRIDQVESLMPLMDMKVLNIFEEETPQIFSL 424
Query: 179 CGKGPRSSLRALRHGLEVAEKAVSEL 256
CG+GPRSSLR LR GL + E AVS+L
Sbjct: 425 CGRGPRSSLRILRPGLAITEMAVSQL 450
Score = 27.1 bits (57), Expect = 7.0
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +3
Query: 453 VYPDGIRHIRADKRV 497
V+P+GIRHIR D R+
Sbjct: 517 VHPNGIRHIREDGRI 531
>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 98.7 bits (235), Expect = 2e-21
Identities = 43/66 (65%), Positives = 56/66 (84%)
Frame = +1
Query: 256 PGSPNAVWTVRRNKDEEYDSYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCHAMG 435
PG P+AVWTV++N +E+D+YI+VSF NATLVLSIGE VEEV DSGFL TTP+L+ +G
Sbjct: 451 PGQPSAVWTVKKNVSDEFDAYIVVSFTNATLVLSIGEQVEEVNDSGFLDTTPSLAVSLIG 510
Query: 436 NDALVQ 453
+D+L+Q
Sbjct: 511 DDSLMQ 516
Score = 85.8 bits (203), Expect = 1e-17
Identities = 42/86 (48%), Positives = 58/86 (67%), Gaps = 1/86 (1%)
Frame = +2
Query: 2 DDEPEFSSAMPLEEG-DTFFFAPRPLRNLVLVDEMDSLSPILACHVADLAGEDTPQVYLA 178
D E S+ M EEG FF PR L+NLV +D+++SL P++ V ++ E+TPQ++
Sbjct: 365 DVESSSSNLMETEEGFQPVFFQPRRLKNLVRIDQVESLMPLMDMKVLNIFEEETPQIFSL 424
Query: 179 CGKGPRSSLRALRHGLEVAEKAVSEL 256
CG+GPRSSLR LR GL + E AVS+L
Sbjct: 425 CGRGPRSSLRILRPGLAITEMAVSQL 450
Score = 27.1 bits (57), Expect = 7.0
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +3
Query: 453 VYPDGIRHIRADKRV 497
V+P+GIRHIR D R+
Sbjct: 517 VHPNGIRHIREDGRI 531
>At3g11960.2 68416.m01476 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to Splicing factor 3B subunit 3
(Spliceosome associatedprotein 130) (SAP 130) (SF3b130)
(Pre-mRNA splicing factor SF3b 130kDa subunit)
(SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF
A subunit region
Length = 1329
Score = 49.6 bits (113), Expect = 1e-06
Identities = 24/60 (40%), Positives = 37/60 (61%), Gaps = 1/60 (1%)
Frame = +1
Query: 277 WTVRRNKDEEYDSYIIVSFVNATLVLSIGETVEEVTDS-GFLGTTPTLSCHAMGNDALVQ 453
WTV+ + Y S++++SFV T VLS+G + ++VTDS GF T +C + + LVQ
Sbjct: 468 WTVKMKLTDVYHSFLVLSFVEETRVLSVGLSFKDVTDSVGFQSDVCTFACGLVADGLLVQ 527
Score = 44.0 bits (99), Expect = 6e-05
Identities = 29/99 (29%), Positives = 49/99 (49%)
Frame = +2
Query: 92 VDEMDSLSPILACHVADLAGEDTPQVYLACGKGPRSSLRALRHGLEVAEKAVSELQDHRM 271
+ + +++PIL V D E Q++ CG P SLR +R G+ V EK + ++
Sbjct: 405 MSSIQNIAPILDFSVMDDQNEKRDQIFACCGVTPEGSLRIIRSGINV-EKLLKTAPVYQG 463
Query: 272 LFGQFDEIKMRNTILTSLCHSLMPHLFFPSVRLLKKSLT 388
+ G + +KM+ LT + HS + F R+L L+
Sbjct: 464 ITGTW-TVKMK---LTDVYHSFLVLSFVEETRVLSVGLS 498
>At3g11960.1 68416.m01475 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to Splicing factor 3B subunit 3
(Spliceosome associatedprotein 130) (SAP 130) (SF3b130)
(Pre-mRNA splicing factor SF3b 130kDa subunit)
(SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF
A subunit region
Length = 1379
Score = 49.6 bits (113), Expect = 1e-06
Identities = 24/60 (40%), Positives = 37/60 (61%), Gaps = 1/60 (1%)
Frame = +1
Query: 277 WTVRRNKDEEYDSYIIVSFVNATLVLSIGETVEEVTDS-GFLGTTPTLSCHAMGNDALVQ 453
WTV+ + Y S++++SFV T VLS+G + ++VTDS GF T +C + + LVQ
Sbjct: 526 WTVKMKLTDVYHSFLVLSFVEETRVLSVGLSFKDVTDSVGFQSDVCTFACGLVADGLLVQ 585
Score = 44.0 bits (99), Expect = 6e-05
Identities = 29/99 (29%), Positives = 49/99 (49%)
Frame = +2
Query: 92 VDEMDSLSPILACHVADLAGEDTPQVYLACGKGPRSSLRALRHGLEVAEKAVSELQDHRM 271
+ + +++PIL V D E Q++ CG P SLR +R G+ V EK + ++
Sbjct: 463 MSSIQNIAPILDFSVMDDQNEKRDQIFACCGVTPEGSLRIIRSGINV-EKLLKTAPVYQG 521
Query: 272 LFGQFDEIKMRNTILTSLCHSLMPHLFFPSVRLLKKSLT 388
+ G + +KM+ LT + HS + F R+L L+
Sbjct: 522 ITGTW-TVKMK---LTDVYHSFLVLSFVEETRVLSVGLS 556
>At4g21100.1 68417.m03051 UV-damaged DNA-binding protein, putative
similar to UV-damaged DNA binding protein (GI:12082087)
[Oryza sativa] and damage-specific DNA binding protein
1, Homo sapiens, PIR2:I38908; contains Pfam PF03178 :
CPSF A subunit region
Length = 1088
Score = 48.4 bits (110), Expect = 3e-06
Identities = 22/62 (35%), Positives = 39/62 (62%), Gaps = 2/62 (3%)
Frame = +1
Query: 274 VWTVRRNKDEEYDSYIIVSFVNAT--LVLSIGETVEEVTDSGFLGTTPTLSCHAMGNDAL 447
+W+++ + DE +D++++VSF++ T L ++I + +EE GFL TL CH + L
Sbjct: 402 MWSLKSSIDEAFDTFLVVSFISETRILAMNIEDELEETEIEGFLSEVQTLFCHDAVYNQL 461
Query: 448 VQ 453
VQ
Sbjct: 462 VQ 463
Score = 33.1 bits (72), Expect = 0.11
Identities = 19/57 (33%), Positives = 31/57 (54%)
Frame = +2
Query: 89 LVDEMDSLSPILACHVADLAGEDTPQVYLACGKGPRSSLRALRHGLEVAEKAVSELQ 259
++++ +L PI+ V DL + QV G SLR +R+G+ + E+A ELQ
Sbjct: 341 ILEKYVNLGPIVDFCVVDLERQGQGQVVTCSGAYKDGSLRIVRNGIGINEQASVELQ 397
>At4g05420.1 68417.m00824 UV-damaged DNA-binding protein, putative
similar to UV-damaged DNA binding protein (GI:12082087)
[Oryza sativa]; contains Pfam PF03178 : CPSF A subunit
region
Length = 1088
Score = 47.6 bits (108), Expect = 5e-06
Identities = 21/62 (33%), Positives = 39/62 (62%), Gaps = 2/62 (3%)
Frame = +1
Query: 274 VWTVRRNKDEEYDSYIIVSFVNAT--LVLSIGETVEEVTDSGFLGTTPTLSCHAMGNDAL 447
+W+++ + DE +D++++VSF++ T L +++ + +EE GFL TL CH + L
Sbjct: 402 MWSLKSSIDEAFDTFLVVSFISETRILAMNLEDELEETEIEGFLSQVQTLFCHDAVYNQL 461
Query: 448 VQ 453
VQ
Sbjct: 462 VQ 463
Score = 32.7 bits (71), Expect = 0.14
Identities = 19/51 (37%), Positives = 27/51 (52%)
Frame = +2
Query: 107 SLSPILACHVADLAGEDTPQVYLACGKGPRSSLRALRHGLEVAEKAVSELQ 259
+L PI+ V DL + QV G SLR +R+G+ + E+A ELQ
Sbjct: 347 NLGPIVDFCVVDLERQGQGQVVTCSGAFKDGSLRVVRNGIGINEQASVELQ 397
>At5g51660.1 68418.m06405 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to SP|Q9EPU4 Cleavage and
polyadenylation specificity factor, 160 kDa subunit
(CPSF 160 kDa subunit) {Mus musculus}; contains Pfam
profile PF03178: CPSF A subunit region
Length = 1442
Score = 30.7 bits (66), Expect = 0.57
Identities = 12/31 (38%), Positives = 21/31 (67%)
Frame = +1
Query: 298 DEEYDSYIIVSFVNATLVLSIGETVEEVTDS 390
++EY +Y+I+S T+VL + + EVT+S
Sbjct: 600 EDEYHAYLIISLEARTMVLETADLLTEVTES 630
>At4g29490.1 68417.m04208 Xaa-Pro dipeptidase, putative / prolidase,
putative / imidodipeptidase, putative similar to
SP|P12955 Xaa-Pro dipeptidase (EC 3.4.13.9) (X-Pro
dipeptidase) (Proline dipeptidase) (Prolidase)
(Imidodipeptidase) {Homo sapiens}; contains Pfam
profiles PF00557: metallopeptidase family M24, PF05195:
Aminopeptidase P, N-terminal domain
Length = 333
Score = 29.9 bits (64), Expect = 1.00
Identities = 23/76 (30%), Positives = 35/76 (46%), Gaps = 3/76 (3%)
Frame = +2
Query: 8 EPEFSSAMPLEEGDTFFFAPR-PLRNLVLVDEMDSLSPILACHVADLA--GEDTPQVYLA 178
EP+F A+ + G + F PR P V + E+ LS ++ D+ ++ QV+
Sbjct: 73 EPDFYGAIDIGSGKSILFIPRLPDDYAVWLGEIKPLSHFKETYMVDMVFYVDEIIQVFNE 132
Query: 179 CGKGPRSSLRALRHGL 226
KG L L HGL
Sbjct: 133 QFKGSGKPLLYLLHGL 148
>At5g28480.1 68418.m03462 hypothetical protein
Length = 1230
Score = 29.5 bits (63), Expect = 1.3
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = +2
Query: 11 PEFSSAMPLEEGDTFFFAPRPLRNLVLVDEMDSLSPILACHVAD 142
P +S +P EGDT F R ++NL + + + S ILAC A+
Sbjct: 287 PSIASLLP-SEGDTAVFTERTIKNLSKLRPIKT-STILACESAE 328
>At2g12100.1 68415.m01300 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At5g28270, At2g05450,
At1g45090, At2g16180, At2g06750
Length = 1224
Score = 29.5 bits (63), Expect = 1.3
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = +2
Query: 11 PEFSSAMPLEEGDTFFFAPRPLRNLVLVDEMDSLSPILACHVAD 142
P +S +P EGDT F R ++NL + + + S ILAC A+
Sbjct: 296 PSIASLLP-SEGDTAVFTERTIKNLSKLRPIKT-STILACESAE 337
>At1g45090.1 68414.m05169 Ulp1 protease family protein similar to
At5g28270, At2g12100, At2g05450, At2g16180, At2g06750;
contains Pfam profile PF02902: Ulp1 protease family,
C-terminal catalytic domain
Length = 1210
Score = 29.5 bits (63), Expect = 1.3
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = +2
Query: 11 PEFSSAMPLEEGDTFFFAPRPLRNLVLVDEMDSLSPILACHVAD 142
P +S +P EGDT F R ++NL + + + S ILAC A+
Sbjct: 287 PSIASLLP-SEGDTAVFTERTIKNLSKLRPIKT-STILACESAE 328
>At1g62970.1 68414.m07110 DNAJ heat shock N-terminal
domain-containing protein low similarity to AHM1
[Triticum aestivum] GI:6691467; contains Pfam profile
PF00226: DnaJ domain
Length = 797
Score = 29.1 bits (62), Expect = 1.7
Identities = 12/31 (38%), Positives = 16/31 (51%)
Frame = -2
Query: 489 CQHEYVLYHQDILY*SVITHCMTTKCRCSSQ 397
C + YVLY I+Y + C T CR + Q
Sbjct: 649 CPYCYVLYEYPIIYEESVLKCQTKSCRRAYQ 679
>At1g03670.1 68414.m00346 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 616
Score = 28.7 bits (61), Expect = 2.3
Identities = 22/85 (25%), Positives = 38/85 (44%), Gaps = 4/85 (4%)
Frame = +1
Query: 253 APGSPNAV-WTVRRNKD---EEYDSYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLS 420
AP PN + TV ++ E Y + V ATLV ++ G++ + P L
Sbjct: 423 APHGPNLIPLTVSQSSKQSPERYKDSVNTLMVTATLVATVTFAAGLTLPGGYMSSAPHLG 482
Query: 421 CHAMGNDALVQYILMV*DIFVLTNV 495
A+ N + L++ +I + T+V
Sbjct: 483 MAALVNKLNFKVFLLLNNIAMCTSV 507
>At5g65440.1 68418.m08230 expressed protein
Length = 1050
Score = 26.6 bits (56), Expect = 9.3
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +2
Query: 44 GDTFFFAPRPLRNLVLVDEMDSLSPILACHVADLA 148
G+TF F P L + ++++ DS + +L VA LA
Sbjct: 347 GETFIFLPDSLGDDLIIEVRDSKAQLLGRVVAQLA 381
>At5g43440.1 68418.m05311 2-oxoglutarate-dependent dioxygenase,
putative similar to 2A6 (GI:599622) and tomato ethylene
synthesis regulatory protein E8 (SP|P10967)
Length = 365
Score = 26.6 bits (56), Expect = 9.3
Identities = 12/30 (40%), Positives = 21/30 (70%)
Frame = +1
Query: 310 DSYIIVSFVNATLVLSIGETVEEVTDSGFL 399
DS++ V+ V LV+SIG+ ++ +T+ FL
Sbjct: 261 DSWVDVTPVPGALVISIGDFMQLITNDKFL 290
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,663,935
Number of Sequences: 28952
Number of extensions: 245257
Number of successful extensions: 628
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 610
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 626
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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