Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00169
(381 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35530.1 68418.m04226 40S ribosomal protein S3 (RPS3C) 135 8e-33
At3g53870.1 68416.m05951 40S ribosomal protein S3 (RPS3B) riboso... 134 2e-32
At2g31610.1 68415.m03862 40S ribosomal protein S3 (RPS3A) 134 2e-32
At3g06190.2 68416.m00712 speckle-type POZ protein-related simila... 33 0.064
At3g06190.1 68416.m00711 speckle-type POZ protein-related simila... 33 0.064
At1g64070.1 68414.m07258 disease resistance protein (TIR-NBS-LRR... 29 1.0
At5g19000.1 68418.m02257 speckle-type POZ protein-related contai... 29 1.4
At4g36190.1 68417.m05149 serine carboxypeptidase S28 family prot... 29 1.4
At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE) i... 29 1.4
At2g25150.1 68415.m03008 transferase family protein similar to 1... 28 2.4
At3g09850.1 68416.m01175 D111/G-patch domain-containing protein ... 27 3.2
At5g51270.1 68418.m06356 protein kinase family protein contains ... 27 4.2
At5g62620.1 68418.m07859 galactosyltransferase family protein co... 27 5.6
At2g24860.1 68415.m02973 chaperone protein dnaJ-related similar ... 27 5.6
At1g56240.1 68414.m06465 SKP1 interacting partner 3-related cont... 27 5.6
At1g22940.1 68414.m02867 thiamin biosynthesis protein, putative ... 27 5.6
At4g36195.1 68417.m05150 serine carboxypeptidase S28 family prot... 26 7.4
At1g50430.1 68414.m05652 7-dehydrocholesterol reductase / 7-DHC ... 26 7.4
At1g18190.1 68414.m02262 expressed protein similar to golgin-84 ... 26 7.4
At5g57100.1 68418.m07129 transporter-related low similarity to G... 26 9.7
At4g24275.1 68417.m03485 expressed protein 26 9.7
At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containi... 26 9.7
At2g25590.1 68415.m03065 agenet domain-containing protein contai... 26 9.7
>At5g35530.1 68418.m04226 40S ribosomal protein S3 (RPS3C)
Length = 248
Score = 135 bits (327), Expect = 8e-33
Identities = 66/78 (84%), Positives = 72/78 (92%)
Frame = +3
Query: 24 ISKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGR 203
ISKKRKFV DGVF AELNE LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGR
Sbjct: 5 ISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGR 64
Query: 204 RIRELTSVVQKRFNIPEN 257
RIRELTS+VQKRF P++
Sbjct: 65 RIRELTSLVQKRFKFPQD 82
Score = 83.8 bits (198), Expect = 3e-17
Identities = 39/43 (90%), Positives = 42/43 (97%)
Frame = +2
Query: 251 RESVELYAEKVATRGLCAIAQAESLRYKLIGGLAVRRACYGVL 379
++SVELYAEKVA RGLCAIAQAESLRYKL+GGLAVRRACYGVL
Sbjct: 81 QDSVELYAEKVANRGLCAIAQAESLRYKLLGGLAVRRACYGVL 123
>At3g53870.1 68416.m05951 40S ribosomal protein S3 (RPS3B) ribosomal
protein S3a - Xenopus laevis, PIR:R3XL3A
Length = 249
Score = 134 bits (324), Expect = 2e-32
Identities = 66/76 (86%), Positives = 70/76 (92%)
Frame = +3
Query: 24 ISKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGR 203
ISKKRKFV DGVF AELNE LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGR
Sbjct: 5 ISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGR 64
Query: 204 RIRELTSVVQKRFNIP 251
RIRELTS+VQKRF P
Sbjct: 65 RIRELTSLVQKRFKFP 80
Score = 81.0 bits (191), Expect = 2e-16
Identities = 38/42 (90%), Positives = 40/42 (95%)
Frame = +2
Query: 254 ESVELYAEKVATRGLCAIAQAESLRYKLIGGLAVRRACYGVL 379
+SVELYAEKV RGLCAIAQAESLRYKL+GGLAVRRACYGVL
Sbjct: 82 DSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVL 123
>At2g31610.1 68415.m03862 40S ribosomal protein S3 (RPS3A)
Length = 250
Score = 134 bits (324), Expect = 2e-32
Identities = 66/76 (86%), Positives = 70/76 (92%)
Frame = +3
Query: 24 ISKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGR 203
ISKKRKFV DGVF AELNE LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGR
Sbjct: 5 ISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGR 64
Query: 204 RIRELTSVVQKRFNIP 251
RIRELTS+VQKRF P
Sbjct: 65 RIRELTSLVQKRFKFP 80
Score = 81.0 bits (191), Expect = 2e-16
Identities = 38/42 (90%), Positives = 40/42 (95%)
Frame = +2
Query: 254 ESVELYAEKVATRGLCAIAQAESLRYKLIGGLAVRRACYGVL 379
+SVELYAEKV RGLCAIAQAESLRYKL+GGLAVRRACYGVL
Sbjct: 82 DSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVL 123
>At3g06190.2 68416.m00712 speckle-type POZ protein-related similar
to SPOP (novel nuclear speckle-type protein)
(SP:O43791) [Homo sapiens]; contains Pfam PF00651 :
BTB/POZ domain; contains Pfam PF00917: MATH domain
Length = 295
Score = 33.1 bits (72), Expect = 0.064
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = -2
Query: 221 SELTDSASFLSEHTLCPGGHNNDLRADGSD 132
SEL + + LSEH+L GH +L ADG D
Sbjct: 255 SELLEYVARLSEHSLTSSGHRKELFADGCD 284
>At3g06190.1 68416.m00711 speckle-type POZ protein-related similar
to SPOP (novel nuclear speckle-type protein)
(SP:O43791) [Homo sapiens]; contains Pfam PF00651 :
BTB/POZ domain; contains Pfam PF00917: MATH domain
Length = 406
Score = 33.1 bits (72), Expect = 0.064
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = -2
Query: 221 SELTDSASFLSEHTLCPGGHNNDLRADGSD 132
SEL + + LSEH+L GH +L ADG D
Sbjct: 366 SELLEYVARLSEHSLTSSGHRKELFADGCD 395
>At1g64070.1 68414.m07258 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 997
Score = 29.1 bits (62), Expect = 1.0
Identities = 18/49 (36%), Positives = 25/49 (51%)
Frame = +2
Query: 230 TEAIQHSRESVELYAEKVATRGLCAIAQAESLRYKLIGGLAVRRACYGV 376
T+AI+ SR S+ L ++ A+ G C E L+ K G V YGV
Sbjct: 61 TQAIRESRISIVLLSKNYASSGWCLDELLEILKCKDDMGQIVMTVFYGV 109
>At5g19000.1 68418.m02257 speckle-type POZ protein-related contains
Pfam PF00651 : BTB/POZ domain; contains Pfam PF00917:
MATH domain; similar to Speckle-type POZ protein
(SP:O43791) [Homo sapiens]
Length = 442
Score = 28.7 bits (61), Expect = 1.4
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -2
Query: 221 SELTDSASFLSEHTLCPGGHNNDLRADGSD 132
+EL + LSEH++ GH ++ ADG D
Sbjct: 402 TELLQYVARLSEHSVIVSGHRKEIFADGCD 431
>At4g36190.1 68417.m05149 serine carboxypeptidase S28 family protein
contains Pfam PF05577: Serine carboxypeptidase S28
Length = 493
Score = 28.7 bits (61), Expect = 1.4
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = +1
Query: 25 FQRSENLLEMGFSRQNSMSSSLGSWPRTATPAWKCGSLPSA 147
F RS N+ F S S +L +W R P CGSL S+
Sbjct: 161 FNRSSNVENPWFFFGVSYSGALSAWFRLKFPHLTCGSLASS 201
>At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE)
identical to C2H2 zinc-finger protein SERRATE
GI:14486602 from [Arabidopsis thaliana]
Length = 720
Score = 28.7 bits (61), Expect = 1.4
Identities = 24/74 (32%), Positives = 32/74 (43%), Gaps = 2/74 (2%)
Frame = -2
Query: 299 KGHE*PPFQHTILQILWNVESLLYYGSELTDSASFLSEHTLCPGGHNNDLRADGSDPHF- 123
KG E T++ LW V L YYG T+ A L H G +D + D ++ F
Sbjct: 395 KGLEGVELLDTLVTYLWRVHGLDYYGKVETNEAKGL-RHVRAE-GKVSDAKGDENESKFD 452
Query: 122 -HAGVAVLGQLPSE 84
H + GQ P E
Sbjct: 453 SHWQERLKGQDPLE 466
>At2g25150.1 68415.m03008 transferase family protein similar to
10-deacetylbaccatin III-10-O-acetyl transferase
[gi:6746554], 2-debenzoyl-7,13-diacetylbaccatin
III-2-O-benzoyl transferase [gi:11559716] from Taxus
cuspidata; contains Pfam transferase family domain
PF00248; contains EST gb:R65039
Length = 461
Score = 27.9 bits (59), Expect = 2.4
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -2
Query: 152 LRADGSDPHFHAGVAVLGQLPSEELIEF 69
L+ SDP GV V+ +LP + ++EF
Sbjct: 418 LKPSKSDPSMEGGVKVIMKLPRDAMVEF 445
>At3g09850.1 68416.m01175 D111/G-patch domain-containing protein
contains Pfam profile PF01585: G-patch domain
Length = 781
Score = 27.5 bits (58), Expect = 3.2
Identities = 15/59 (25%), Positives = 29/59 (49%)
Frame = +3
Query: 30 KKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGRR 206
K RK G + KA + E+ ++ GYSG P+ + ++ +S+ G+K ++
Sbjct: 349 KTRKLTGIRLQKASM-EYGKKKTTRSGYSGHGKSTNPLAMDDLMFVKDPRSLSGKKNKK 406
>At5g51270.1 68418.m06356 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain
Length = 819
Score = 27.1 bits (57), Expect = 4.2
Identities = 14/29 (48%), Positives = 15/29 (51%)
Frame = +1
Query: 133 SLPSARRSLLWPPGHRVCSERKDAESVSS 219
S P RS+ W P R ERKDA S S
Sbjct: 295 SSPETSRSVSWNPQFRDFDERKDAMSSMS 323
>At5g62620.1 68418.m07859 galactosyltransferase family protein
contains Pfam profile: PF01762 galactosyltransferase
Length = 681
Score = 26.6 bits (56), Expect = 5.6
Identities = 15/57 (26%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +1
Query: 55 GFSRQNSMSSSLGSWPRTATPAWKCGSLPSARRS-LLWPPGHRVCSERKDAESVSSL 222
G R+ + SSL P T P+ K GS+ + + + W G ++ E + +++ +L
Sbjct: 99 GLRRRTRILSSLRFDPETFNPSSKDGSVELHKSAKVAWEVGRKIWEELESGKTLKAL 155
>At2g24860.1 68415.m02973 chaperone protein dnaJ-related similar to
Tsi1-interacting protein TSIP1 (GI:4337001) [Nicotiana
tabacum]
Length = 144
Score = 26.6 bits (56), Expect = 5.6
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -1
Query: 321 DSAWAIAQRPRVATFSAYNSTDSLEC 244
D AW I+Q+P S+ NS +EC
Sbjct: 62 DQAWIISQQPSPVGCSSCNSKGHVEC 87
>At1g56240.1 68414.m06465 SKP1 interacting partner 3-related
contains similarity to SKP1 interacting partner 3
GI:10716951 from [Arabidopsis thaliana]
Length = 284
Score = 26.6 bits (56), Expect = 5.6
Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Frame = +3
Query: 6 IMAVNNISKKR--KFVGDGVFKAELNEFLTRELAED--GYSGVEVRVTPIRSEIII 161
+ AV K+R K DG + EL EF+TRE +D S EV+ ++ I+I
Sbjct: 218 VEAVGGDGKRREPKARDDGWLEIELGEFVTREGEDDEVNMSLTEVKGYQLKGGIVI 273
>At1g22940.1 68414.m02867 thiamin biosynthesis protein, putative
strong similarity to hydroxymethylpyrimidine
kinase/thiamin-phosphate pyrophosphorylase (BTH1)
GI:7488455 from [Brassica napus]
Length = 522
Score = 26.6 bits (56), Expect = 5.6
Identities = 17/45 (37%), Positives = 23/45 (51%)
Frame = -2
Query: 191 SEHTLCPGGHNNDLRADGSDPHFHAGVAVLGQLPSEELIEFCLEN 57
S H L P + L++ SD F V G LPS E++E L+N
Sbjct: 78 SVHLLPPEFISEQLKSVLSD--FEFDVVKTGMLPSTEIVEVLLQN 120
>At4g36195.1 68417.m05150 serine carboxypeptidase S28 family protein
contains Pfam PF05577: Serine carboxypeptidase S28
Length = 488
Score = 26.2 bits (55), Expect = 7.4
Identities = 15/41 (36%), Positives = 20/41 (48%)
Frame = +1
Query: 25 FQRSENLLEMGFSRQNSMSSSLGSWPRTATPAWKCGSLPSA 147
F RS ++ F S S +L +W R P CGSL S+
Sbjct: 161 FNRSGDVENPWFFFGASYSGALSAWFRLKFPHLTCGSLASS 201
>At1g50430.1 68414.m05652 7-dehydrocholesterol reductase / 7-DHC
reductase / sterol delta-7-reductase (ST7R) / dwarf5
protein (DWF5) identical to SP|Q9LDU6
7-dehydrocholesterol reductase (EC 1.3.1.21) (7-DHC
reductase) (Sterol delta-7-reductase) (Dwarf5 protein)
{Arabidopsis thaliana}
Length = 432
Score = 26.2 bits (55), Expect = 7.4
Identities = 19/62 (30%), Positives = 26/62 (41%), Gaps = 4/62 (6%)
Frame = +1
Query: 10 WP*TIFQRSENLLEMGFSRQNSMSSSLGSWPRTATPAWK---C-GSLPSARRSLLWPPGH 177
W + Q GF +N + + WPR AWK C G+ + + LL PG
Sbjct: 30 WYTMVHQDGSVTQTFGFFWENGVQGLINIWPRPTLIAWKIIFCYGAFEAILQLLL--PGK 87
Query: 178 RV 183
RV
Sbjct: 88 RV 89
>At1g18190.1 68414.m02262 expressed protein similar to golgin-84
{Homo sapiens} (GI:4191344)
Length = 668
Score = 26.2 bits (55), Expect = 7.4
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = +2
Query: 224 RSTEAIQHSRESVELYAEKVATRGLCAIAQAESLRYKLIG 343
R + + R ++AEKVAT+ + I + E++ +L G
Sbjct: 306 RERQREERRRRRERVFAEKVATKAVAVIKERENMVARLEG 345
>At5g57100.1 68418.m07129 transporter-related low similarity to
GDP-fucose transporter [Caenorhabditis elegans]
GI:13940504, phosphoenolpyruvate/phosphate translocator
precursor [Mesembryanthemum crystallinum] GI:9295275
Length = 390
Score = 25.8 bits (54), Expect = 9.7
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +3
Query: 141 IRSEIIIMATRTQSVLGEKGRRIRELTSVVQKRFNIPE 254
+R ++ + R S GE+G+ + +L + + RF PE
Sbjct: 12 LRQDVKKILKRKDSDAGERGKALEDLRASLFNRFRSPE 49
>At4g24275.1 68417.m03485 expressed protein
Length = 126
Score = 25.8 bits (54), Expect = 9.7
Identities = 11/28 (39%), Positives = 14/28 (50%)
Frame = +1
Query: 232 RSDSTFQRICRIVC*KGGYSWPLRYRPG 315
RS S +R CRI +WPL + G
Sbjct: 62 RSSSVSERYCRIYDQSSATTWPLPFHEG 89
>At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 617
Score = 25.8 bits (54), Expect = 9.7
Identities = 15/37 (40%), Positives = 22/37 (59%)
Frame = +1
Query: 43 LLEMGFSRQNSMSSSLGSWPRTATPAWKCGSLPSARR 153
L EM +++ +S+ LGS + + KCG L SARR
Sbjct: 251 LEEMAITKKIGLSTFLGS--KLISMYGKCGDLDSARR 285
>At2g25590.1 68415.m03065 agenet domain-containing protein contains
Pfam PF05641: Agenet domain
Length = 381
Score = 25.8 bits (54), Expect = 9.7
Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 2/46 (4%)
Frame = -3
Query: 319 FGLGDSAKATSS--HLFSIQFYRFSGMLNRFCTTEVSSRILRPFSP 188
+G+ SA+ S H +++++Y F N V +I+RP P
Sbjct: 19 YGVWRSAEIISGNGHTYNVRYYSFEIANNEVVEDRVPRKIIRPCPP 64
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,301,867
Number of Sequences: 28952
Number of extensions: 158294
Number of successful extensions: 518
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 501
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 518
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 527724392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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