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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00167
         (771 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g10730.1 68414.m01223 clathrin adaptor complexes medium subun...    83   1e-16
At5g46630.2 68418.m05740 clathrin adaptor complexes medium subun...    83   2e-16
At5g46630.1 68418.m05741 clathrin adaptor complexes medium subun...    83   2e-16
At1g60780.1 68414.m06842 clathrin adaptor complexes medium subun...    83   2e-16
At4g24550.2 68417.m03519 clathrin adaptor complexes medium subun...    82   5e-16
At4g24550.1 68417.m03518 clathrin adaptor complexes medium subun...    82   5e-16
At1g56590.1 68414.m06508 clathrin adaptor complexes medium subun...    64   1e-10
At4g35410.2 68417.m05030 clathrin adaptor complex small chain fa...    36   0.030
At2g17380.1 68415.m02007 clathrin assembly protein AP19 identica...    34   0.12 
At5g23700.1 68418.m02778 hypothetical protein                          31   0.84 
At4g33140.1 68417.m04721 expressed protein                             29   2.6  
At4g20060.1 68417.m02935 expressed protein  ; expression support...    29   2.6  
At5g40480.1 68418.m04909 expressed protein ; expression supporte...    29   4.5  
At5g01270.1 68418.m00036 double-stranded RNA-binding domain (DsR...    29   4.5  
At3g11460.1 68416.m01397 pentatricopeptide (PPR) repeat-containi...    29   4.5  
At5g19740.1 68418.m02347 peptidase M28 family protein ileal pept...    28   5.9  
At2g23050.1 68415.m02748 phototropic-responsive NPH3 family prot...    28   5.9  
At5g16210.1 68418.m01894 HEAT repeat-containing protein contains...    28   7.9  

>At1g10730.1 68414.m01223 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 428

 Score = 83.4 bits (197), Expect = 1e-16
 Identities = 34/81 (41%), Positives = 57/81 (70%)
 Frame = +3

Query: 267 QVRSPVTNIARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENI 446
           Q   PV      ++  ++ +NI+L   ++QN NAA +  FL +++DV + YF ++ EE++
Sbjct: 47  QSNDPVAYDNGVTYMFVQHSNIYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESL 106

Query: 447 KNNFVLIYELLDEILDFGYPQ 509
           ++NFV++YELLDE++DFGYPQ
Sbjct: 107 RDNFVVVYELLDEMMDFGYPQ 127



 Score = 42.7 bits (96), Expect = 3e-04
 Identities = 15/32 (46%), Positives = 26/32 (81%)
 Frame = +2

Query: 599 VTGQIGWRRESIKYRRNELFLDVLEYVHLLMS 694
           VT  + WR E +K+++NE+FLDV+E V++L++
Sbjct: 155 VTNSVSWRSEGLKFKKNEVFLDVIESVNILVN 186


>At5g46630.2 68418.m05740 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 441

 Score = 83.0 bits (196), Expect = 2e-16
 Identities = 38/80 (47%), Positives = 55/80 (68%), Gaps = 1/80 (1%)
 Frame = +3

Query: 279 PVTNIARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFG-KISEENIKNN 455
           PV  I   SF +++ +N+++  V   N N A  F+F+++ + + +SYFG    E+ I+NN
Sbjct: 49  PVRQIGGCSFVYMRISNVYIVIVVSSNANVACGFKFVVEAVALFKSYFGGAFDEDAIRNN 108

Query: 456 FVLIYELLDEILDFGYPQIL 515
           FVLIYELLDEI+DFGYPQ L
Sbjct: 109 FVLIYELLDEIMDFGYPQNL 128



 Score = 69.3 bits (162), Expect = 3e-12
 Identities = 33/69 (47%), Positives = 49/69 (71%), Gaps = 5/69 (7%)
 Frame = +2

Query: 503 PSNSDTGVLKTFITQQGIKS--ASKEEQAQI---TSQVTGQIGWRRESIKYRRNELFLDV 667
           P N    +LK +ITQ+G++S  +SK +   +   T QVTG +GWRRE + Y++NE+FLD+
Sbjct: 125 PQNLSPEILKLYITQEGVRSPFSSKPKDKPVPNATLQVTGAVGWRREGLAYKKNEVFLDI 184

Query: 668 LEYVHLLMS 694
           +E V+LLMS
Sbjct: 185 VESVNLLMS 193



 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 18/39 (46%), Positives = 27/39 (69%)
 Frame = +1

Query: 139 PTMIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVI 255
           P     ++  N +G+VLI+R YRDD+G N VDAFR +++
Sbjct: 2   PVAASAIYFLNLRGDVLINRTYRDDVGGNMVDAFRTHIM 40


>At5g46630.1 68418.m05741 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 438

 Score = 83.0 bits (196), Expect = 2e-16
 Identities = 38/80 (47%), Positives = 55/80 (68%), Gaps = 1/80 (1%)
 Frame = +3

Query: 279 PVTNIARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFG-KISEENIKNN 455
           PV  I   SF +++ +N+++  V   N N A  F+F+++ + + +SYFG    E+ I+NN
Sbjct: 49  PVRQIGGCSFVYMRISNVYIVIVVSSNANVACGFKFVVEAVALFKSYFGGAFDEDAIRNN 108

Query: 456 FVLIYELLDEILDFGYPQIL 515
           FVLIYELLDEI+DFGYPQ L
Sbjct: 109 FVLIYELLDEIMDFGYPQNL 128



 Score = 69.3 bits (162), Expect = 3e-12
 Identities = 33/69 (47%), Positives = 49/69 (71%), Gaps = 5/69 (7%)
 Frame = +2

Query: 503 PSNSDTGVLKTFITQQGIKS--ASKEEQAQI---TSQVTGQIGWRRESIKYRRNELFLDV 667
           P N    +LK +ITQ+G++S  +SK +   +   T QVTG +GWRRE + Y++NE+FLD+
Sbjct: 125 PQNLSPEILKLYITQEGVRSPFSSKPKDKPVPNATLQVTGAVGWRREGLAYKKNEVFLDI 184

Query: 668 LEYVHLLMS 694
           +E V+LLMS
Sbjct: 185 VESVNLLMS 193



 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 18/39 (46%), Positives = 27/39 (69%)
 Frame = +1

Query: 139 PTMIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVI 255
           P     ++  N +G+VLI+R YRDD+G N VDAFR +++
Sbjct: 2   PVAASAIYFLNLRGDVLINRTYRDDVGGNMVDAFRTHIM 40


>At1g60780.1 68414.m06842 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 428

 Score = 83.0 bits (196), Expect = 2e-16
 Identities = 33/81 (40%), Positives = 57/81 (70%)
 Frame = +3

Query: 267 QVRSPVTNIARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENI 446
           Q   PV      ++  ++ +N++L   ++QN NAA +  FL +++DV + YF ++ EE++
Sbjct: 47  QSNDPVAYDNGVTYMFVQHSNVYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESL 106

Query: 447 KNNFVLIYELLDEILDFGYPQ 509
           ++NFV++YELLDE++DFGYPQ
Sbjct: 107 RDNFVVVYELLDEMMDFGYPQ 127



 Score = 43.6 bits (98), Expect = 1e-04
 Identities = 21/64 (32%), Positives = 36/64 (56%)
 Frame = +2

Query: 503 PSNSDTGVLKTFITQQGIKSASKEEQAQITSQVTGQIGWRRESIKYRRNELFLDVLEYVH 682
           P  ++  +L  FI     +    E   +    VT  + WR E I+Y++NE+FLDV+E V+
Sbjct: 126 PQYTEARILSEFIKTDAYRM---EVTQRPPMAVTNAVSWRSEGIQYKKNEVFLDVIENVN 182

Query: 683 LLMS 694
           +L++
Sbjct: 183 ILVN 186


>At4g24550.2 68417.m03519 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 451

 Score = 81.8 bits (193), Expect = 5e-16
 Identities = 32/77 (41%), Positives = 54/77 (70%)
 Frame = +3

Query: 279 PVTNIARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNF 458
           P+ N+   ++FH+K   ++  A T+ NV+ ++V E L +I  V++ Y G ++E++ + NF
Sbjct: 50  PIFNVDGVNYFHVKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNEDSFRKNF 109

Query: 459 VLIYELLDEILDFGYPQ 509
           VL+YELLDE++DFGY Q
Sbjct: 110 VLVYELLDEVIDFGYVQ 126


>At4g24550.1 68417.m03518 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 380

 Score = 81.8 bits (193), Expect = 5e-16
 Identities = 32/77 (41%), Positives = 54/77 (70%)
 Frame = +3

Query: 279 PVTNIARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNF 458
           P+ N+   ++FH+K   ++  A T+ NV+ ++V E L +I  V++ Y G ++E++ + NF
Sbjct: 50  PIFNVDGVNYFHVKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNEDSFRKNF 109

Query: 459 VLIYELLDEILDFGYPQ 509
           VL+YELLDE++DFGY Q
Sbjct: 110 VLVYELLDEVIDFGYVQ 126


>At1g56590.1 68414.m06508 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 415

 Score = 63.7 bits (148), Expect = 1e-10
 Identities = 27/66 (40%), Positives = 43/66 (65%)
 Frame = +3

Query: 309 FHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEI 488
           F I R  I   A ++  +   M  EFL ++ DV+  Y G ++E+ IK+NF+++YELLDE+
Sbjct: 58  FQIVRDGITFLACSQVEMPPLMAIEFLCRVADVLSEYLGGLNEDLIKDNFIIVYELLDEM 117

Query: 489 LDFGYP 506
           +D G+P
Sbjct: 118 IDNGFP 123


>At4g35410.2 68417.m05030 clathrin adaptor complex small chain
           family protein contains Pfam profile: PF01217 clathrin
           adaptor complex small chain
          Length = 162

 Score = 35.9 bits (79), Expect = 0.030
 Identities = 17/59 (28%), Positives = 30/59 (50%)
 Frame = +3

Query: 324 ANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFG 500
           A+++      Q  N   V E +   ++++  YFG + E ++  NF   Y +LDE+L  G
Sbjct: 63  ASLYFCMCIDQEDNELEVLEIIHHYVEILDRYFGSVCELDLIFNFHKAYYILDELLIAG 121


>At2g17380.1 68415.m02007 clathrin assembly protein AP19 identical
           to clathrin assembly protein AP19 GI:2231698 from
           [Arabidopsis thaliana]
          Length = 161

 Score = 33.9 bits (74), Expect = 0.12
 Identities = 16/59 (27%), Positives = 30/59 (50%)
 Frame = +3

Query: 324 ANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFG 500
           A+++      +  N   V E +   ++++  YFG + E ++  NF   Y +LDE+L  G
Sbjct: 63  ASLYFCMCIDEADNELEVLEIIHHYVEILDRYFGSVCELDLIFNFHKAYYILDELLIAG 121


>At5g23700.1 68418.m02778 hypothetical protein
          Length = 572

 Score = 31.1 bits (67), Expect = 0.84
 Identities = 18/53 (33%), Positives = 27/53 (50%), Gaps = 3/53 (5%)
 Frame = -2

Query: 266 LASMITFTLNASTALRPISSR*TLEINTSPLWL*TN---KPPIIVGEASHSFK 117
           +   +T   ++ST  RP +   +   N SPLW+      KPP+I+    HSFK
Sbjct: 112 ITEQVTSVRSSSTG-RPSTFSRSSTPNASPLWMPPKASLKPPVIIPPIDHSFK 163


>At4g33140.1 68417.m04721 expressed protein
          Length = 353

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 21/62 (33%), Positives = 29/62 (46%)
 Frame = -1

Query: 588 IWACSSLEADLMPC*VMNVFRTPVSEFEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIA 409
           IW CS  EADL    V   F+T   +   +  P +H+  H+  + Y   S+  S QN I 
Sbjct: 191 IWNCSRNEADLR---VHEFFKTSYFKKGIHPLPGAHKTLHKLSK-YCDMSVVTSRQNAIK 246

Query: 408 SH 403
            H
Sbjct: 247 EH 248


>At4g20060.1 68417.m02935 expressed protein  ; expression supported by
            MPSS
          Length = 1134

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 18/67 (26%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
 Frame = -2

Query: 494  VQDLIKQL--IDQNKVI-LDVLFRNLAKIRLHHINDLQQELKYHGRVHILLCDCCQPNIC 324
            V+DL+++L  +D N    L++L      +   H+    Q L+  G+V +LL  C     C
Sbjct: 844  VEDLVRRLWKVDPNVCEKLNILVNTNESLNCFHLQSRNQVLRVCGKVKMLLSICRDALSC 903

Query: 323  TLDMEER 303
            T  ++ +
Sbjct: 904  TYGLQNQ 910


>At5g40480.1 68418.m04909 expressed protein ; expression supported by
            MPSS
          Length = 1919

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 15/43 (34%), Positives = 23/43 (53%)
 Frame = +1

Query: 172  HKGEVLISRVYRDDIGRNAVDAFRVNVIMLASRCDHLLPTLHA 300
            +KG + IS + RDD G      ++VNV+      D ++ TL A
Sbjct: 1757 NKGRLSISLIKRDDFGIAGHAQYKVNVLRSEQFTDRIIITLPA 1799


>At5g01270.1 68418.m00036 double-stranded RNA-binding domain
           (DsRBD)-containing protein contains Pfam profile
           PF00035: Double-stranded RNA binding motif
          Length = 771

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
 Frame = -1

Query: 297 VQCW*QVIAPAGEHD--HIHSECVHSITTNIISVDSRDQHLAFMVVNEQ 157
           V+C  +  AP    +  H+H+ C H + T ++ +   + HL  M   E+
Sbjct: 64  VRCKLESSAPVKSQELMHLHAVCFHELKTAVVMLGDEEIHLVAMPSKEK 112


>At3g11460.1 68416.m01397 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 623

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 22/67 (32%), Positives = 31/67 (46%), Gaps = 2/67 (2%)
 Frame = +2

Query: 509 NSDTGVLKTFITQQGIKSASKEEQAQITSQ--VTGQIGWRRESIKYRRNELFLDVLEYVH 682
           +S+  VL +FIT   +K  S E   ++  +  V G I W      Y +N L  DVLE   
Sbjct: 188 DSEVAVLNSFITMY-MKCGSVEAGRRLFDEMPVKGLITWNAVISGYSQNGLAYDVLELYE 246

Query: 683 LLMSPQV 703
            + S  V
Sbjct: 247 QMKSSGV 253


>At5g19740.1 68418.m02347 peptidase M28 family protein ileal
           peptidase I100 - Rattus norvegicus, EMBL:AF009921;
           contains Pfam profiles PF04389: Peptidase family M28,
           PF02225: PA domain
          Length = 681

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 15/40 (37%), Positives = 24/40 (60%), Gaps = 3/40 (7%)
 Frame = +1

Query: 169 NHKGEVLISR---VYRDDIGRNAVDAFRVNVIMLASRCDH 279
           N  G V+I+R   +YR DI +NA +A  V V++   + D+
Sbjct: 166 NVSGAVVIARYGQIYRGDIVKNAYEAGAVGVVIYTDKRDY 205


>At2g23050.1 68415.m02748 phototropic-responsive NPH3 family protein
           contains NPH3 family domain, Pfam:PF03000
          Length = 481

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
 Frame = +3

Query: 228 CGRIQSE-CDHARQQVRSPVTNIARTSFFHIKRAN 329
           C ++ +E C+HA Q  R P+  I +  FF   RAN
Sbjct: 418 CRKLSAEACEHAVQNERLPMRVIVQVLFFEQIRAN 452


>At5g16210.1 68418.m01894 HEAT repeat-containing protein contains
           Pfam profile PF02985: HEAT repeat
          Length = 1180

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 25/102 (24%), Positives = 47/102 (46%), Gaps = 2/102 (1%)
 Frame = -2

Query: 509 LRVTKVQDLIKQLI-DQNKVILDVLFRNLAKIRLHHINDLQQELKYHGRVHILLCDCCQP 333
           +R + +  +++QLI D   V+ +    NLA + L    +  +  K    +  L+CD    
Sbjct: 567 IRDSLILSIVQQLIEDSATVVREAAAHNLALL-LPLFPNTDKYFKVEEMMFQLICDPSGL 625

Query: 332 NI-CTLDMEERCACNVGNR*SHLLASMITFTLNASTALRPIS 210
            +  TL          GNR  H+L  +++ TL+++    P+S
Sbjct: 626 VVETTLKELLPAVIKWGNRLDHILRGLLSHTLSSAQHCPPLS 667


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,723,333
Number of Sequences: 28952
Number of extensions: 342866
Number of successful extensions: 801
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 764
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 797
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1716774400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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