BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00166 (684 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1105.05 |exg1||glucan 1,3-beta-glucosidase I/II precursor|Sc... 28 1.1 SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schi... 27 2.5 SPAC20G8.01 |cdc17||ATP-dependent DNA ligase Cdc17|Schizosacchar... 26 4.4 SPAC57A7.13 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 26 4.4 SPBC3H7.12 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 4.4 SPBC582.05c |brc1||BRCT domain protein Brc1|Schizosaccharomyces ... 26 4.4 SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1||... 26 5.8 SPCC16C4.08c |skb15||Shk1 kinase binding protein 15|Schizosaccha... 26 5.8 SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 25 7.7 SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 25 7.7 >SPBC1105.05 |exg1||glucan 1,3-beta-glucosidase I/II precursor|Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 28.3 bits (60), Expect = 1.1 Identities = 17/51 (33%), Positives = 24/51 (47%) Frame = -3 Query: 394 SRYFGLTGLLSPTLSMSDLFEPSLKSIRFKGNKSSDAVWGVPDVPGSPRLY 242 S Y +TG S TL+ +FE + S F G S D + G+ RL+ Sbjct: 304 SPYITVTGEWSGTLADCTIFEEGVDSSTFIGPNSGDISTWTDEYKGAVRLF 354 >SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schizosaccharomyces pombe|chr 2|||Manual Length = 451 Score = 27.1 bits (57), Expect = 2.5 Identities = 17/61 (27%), Positives = 34/61 (55%), Gaps = 2/61 (3%) Frame = +1 Query: 82 ETCVQDHDYNLTVATNEDETSCAVLEKIPRAGKRNAEA--EVSDSSSNSCEMDTRSRAEG 255 E+ + D + + +++E E+S + + +E+ E SDSSS+S + ++ S +EG Sbjct: 156 ESSSEGSDSSSSSSSSESESSSEDNDSSSSSSDSESESSSEDSDSSSSSSDSESESSSEG 215 Query: 256 S 258 S Sbjct: 216 S 216 Score = 25.4 bits (53), Expect = 7.7 Identities = 11/24 (45%), Positives = 18/24 (75%) Frame = +1 Query: 187 AEAEVSDSSSNSCEMDTRSRAEGS 258 + +E SDSSS+S + ++ S +EGS Sbjct: 139 SSSEDSDSSSSSSDSESESSSEGS 162 Score = 25.4 bits (53), Expect = 7.7 Identities = 16/61 (26%), Positives = 32/61 (52%), Gaps = 2/61 (3%) Frame = +1 Query: 82 ETCVQDHDYNLTVATNEDETSCAVLEKIPRAGKRNAEAEV--SDSSSNSCEMDTRSRAEG 255 E+ +D+D + + + +E E+S + + +E+ SDSSSNS + + S ++ Sbjct: 228 ESSSEDNDSSSSSSDSESESSSEDSDSSSSSSDSESESSSKDSDSSSNSSDSEDDSSSDS 287 Query: 256 S 258 S Sbjct: 288 S 288 >SPAC20G8.01 |cdc17||ATP-dependent DNA ligase Cdc17|Schizosaccharomyces pombe|chr 1|||Manual Length = 768 Score = 26.2 bits (55), Expect = 4.4 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +1 Query: 163 IPRAGKRNAEAEVSDSSSNSCEMDTRSRAEGSRARPV 273 I A ++ EVS SSS+S +D RS +E + V Sbjct: 32 ISSASHKSEHVEVSQSSSDSKNVDGRSTSEKRKVESV 68 >SPAC57A7.13 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 565 Score = 26.2 bits (55), Expect = 4.4 Identities = 11/24 (45%), Positives = 15/24 (62%), Gaps = 1/24 (4%) Frame = +3 Query: 447 NKALKMMENMGWN-GGALGARGMG 515 N ++KM+ +MGWN G LG G Sbjct: 492 NVSMKMLNSMGWNKGSGLGTNENG 515 >SPBC3H7.12 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 287 Score = 26.2 bits (55), Expect = 4.4 Identities = 10/21 (47%), Positives = 14/21 (66%) Frame = -2 Query: 530 PMALLSHSPRSQGSTVPSHIF 468 P + L+HSPR TV +H+F Sbjct: 172 PFSCLNHSPRQIHKTVRNHLF 192 >SPBC582.05c |brc1||BRCT domain protein Brc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 878 Score = 26.2 bits (55), Expect = 4.4 Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 2/51 (3%) Frame = +3 Query: 291 ELLLPLKR--IDFKLGSNRSDIDSVGDSKPVKPKYRLGRWQIKLLNDMSVP 437 ++ + LKR I+F L SN++ ++G + + LG W+ LLN + P Sbjct: 261 DIFIGLKRDTIEFNLASNKNT--TIGTISWLLNLFVLGSWKSPLLNALHYP 309 >SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1|||Manual Length = 753 Score = 25.8 bits (54), Expect = 5.8 Identities = 13/56 (23%), Positives = 25/56 (44%) Frame = +3 Query: 396 GRWQIKLLNDMSVPVKCNKALKMMENMGWNGGALGARGMG*QSHWYLSSEWHQAPA 563 G W++ L + P+ CN L +E++ G S + + ++W + PA Sbjct: 379 GSWRVNLDTQLIDPLPCNLGLIFLESLSEIGQIYTGYCNRQDSVFKIITKWREKPA 434 >SPCC16C4.08c |skb15||Shk1 kinase binding protein 15|Schizosaccharomyces pombe|chr 3|||Manual Length = 341 Score = 25.8 bits (54), Expect = 5.8 Identities = 15/72 (20%), Positives = 27/72 (37%) Frame = +1 Query: 49 DDLCVTFQRVLETCVQDHDYNLTVATNEDETSCAVLEKIPRAGKRNAEAEVSDSSSNSCE 228 DD +T C+ D D+NL + N E + A + V D ++ Sbjct: 249 DDYLITASSDGSVCIWDKDWNLVIEHNIPEGNRITCMVAMLADSNSEPKNVEDEAAKRQS 308 Query: 229 MDTRSRAEGSRA 264 +D+ + S + Sbjct: 309 LDSETSETSSES 320 >SPBC25H2.03 |||vacuolar protein involved in phosphoinositide metabolism|Schizosaccharomyces pombe|chr 2|||Manual Length = 811 Score = 25.4 bits (53), Expect = 7.7 Identities = 11/27 (40%), Positives = 14/27 (51%) Frame = -3 Query: 109 CSRDPGHKSPIPAETSHRGRRSDPYYT 29 CS P + + E R RR DPY+T Sbjct: 704 CSSTPRTNTILANERLPRSRRDDPYWT 730 >SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 871 Score = 25.4 bits (53), Expect = 7.7 Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Frame = -3 Query: 421 FSNFICHLPSRYFG-LTGLLSPTLSMSDLFEPSLKSIRFKG 302 F FI H+P + G +TG+L P++++S L I+F G Sbjct: 411 FLKFIDHMPPKLLGIITGIL-PSVALSILMSLVPPFIKFLG 450 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,003,489 Number of Sequences: 5004 Number of extensions: 67290 Number of successful extensions: 232 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 219 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 232 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 315915086 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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