Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00163
(673 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) riboso... 123 1e-28
At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B) 121 5e-28
At1g24110.1 68414.m03042 peroxidase, putative similar to peroxid... 32 0.40
At3g24080.1 68416.m03024 KRR1 family protein contains Pfam PF051... 30 1.6
At1g21740.1 68414.m02721 expressed protein contains Pfam domains... 29 2.8
At1g67280.1 68414.m07657 lactoylglutathione lyase, putative / gl... 29 3.7
At2g16390.1 68415.m01876 SNF2 domain-containing protein / helica... 28 6.5
>At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) ribosomal
protein S6, Arabidopsis thaliana, PID:g2662469
Length = 250
Score = 123 bits (296), Expect = 1e-28
Identities = 86/222 (38%), Positives = 116/222 (52%), Gaps = 6/222 (2%)
Frame = +3
Query: 24 MKLNVSYPATGCQKLFEVVDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVAGGNDKQG 203
MK NV+ P TGCQK E+ D+ KLR FY+KR+ EV D LG+E+KGYV ++ GG DKQG
Sbjct: 1 MKFNVANPTTGCQKKLEIDDDQKLRAFYDKRISQEVSGDALGEEFKGYVFKIKGGCDKQG 60
Query: 204 FPMKQGVLTNSRVRLLMQRAT---HVTDRAAMERENVNQFVDVLLTPISRSWLLLLCAG- 371
FPMKQGVLT RVRLL+ R T R ER + ++ +S L+++ G
Sbjct: 61 FPMKQGVLTPGRVRLLLHRGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKKGE 120
Query: 372 --CPGNSWID*WKCTPPSRSQTCFQNP*AVQP*QRR*CTSLCRQTRAPAKEGKENAKPRH 545
PG + + + P R+ + + R + R+ + K+GKE +
Sbjct: 121 NDLPGLTDTEKPRMRGPKRASKIRKLFNLKKEDDVRTYVNTYRR-KFTNKKGKEVS---- 175
Query: 546 KAPKIQRLVTPVVLQXXXXXXXXXXXXXXXXXSSEAEYAKLL 671
KAPKIQRLVTP+ LQ S A+Y KLL
Sbjct: 176 KAPKIQRLVTPLTLQRKRARIADKKKKIAKANSDAADYQKLL 217
Score = 66.9 bits (156), Expect = 1e-11
Identities = 36/66 (54%), Positives = 44/66 (66%), Gaps = 3/66 (4%)
Frame = +1
Query: 367 QGAQEIPGLTDGNVPRRLGPKRASKIRKLFNLSKEDDVRRYV--VKRVLQPRKEKKMLN- 537
+G ++PGLTD PR GPKRASKIRKLFNL KEDDVR YV +R +K K++
Sbjct: 118 KGENDLPGLTDTEKPRMRGPKRASKIRKLFNLKKEDDVRTYVNTYRRKFTNKKGKEVSKA 177
Query: 538 PDIRHL 555
P I+ L
Sbjct: 178 PKIQRL 183
Score = 50.0 bits (114), Expect = 1e-06
Identities = 25/40 (62%), Positives = 31/40 (77%), Gaps = 2/40 (5%)
Frame = +2
Query: 257 KGHSCYRP--RRDGERKRKSVRGCIVDANLSVLALVIVRR 370
+G C+R RR GER+RKSVRGCIV +LSVL LVIV++
Sbjct: 79 RGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKK 118
>At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B)
Length = 249
Score = 121 bits (291), Expect = 5e-28
Identities = 85/222 (38%), Positives = 116/222 (52%), Gaps = 6/222 (2%)
Frame = +3
Query: 24 MKLNVSYPATGCQKLFEVVDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVAGGNDKQG 203
MK NV+ P TGCQK E+ D+ KLR F++KR+ EV D LG+E+KGYV ++ GG DKQG
Sbjct: 1 MKFNVANPTTGCQKKLEIDDDQKLRAFFDKRLSQEVSGDALGEEFKGYVFKIMGGCDKQG 60
Query: 204 FPMKQGVLTNSRVRLLMQRAT---HVTDRAAMERENVNQFVDVLLTPISRSWLLLLCAG- 371
FPMKQGVLT RVRLL+ R T R ER + ++ +S L+++ G
Sbjct: 61 FPMKQGVLTPGRVRLLLHRGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKKGV 120
Query: 372 --CPGNSWID*WKCTPPSRSQTCFQNP*AVQP*QRR*CTSLCRQTRAPAKEGKENAKPRH 545
PG + + + P R+ + + R + R+T K+GK+ +
Sbjct: 121 SDLPGLTDTEKPRMRGPKRASKIRKLFNLGKEDDVRKYVNTYRRT-FTNKKGKKVS---- 175
Query: 546 KAPKIQRLVTPVVLQXXXXXXXXXXXXXXXXXSSEAEYAKLL 671
KAPKIQRLVTP+ LQ S A+Y KLL
Sbjct: 176 KAPKIQRLVTPLTLQRKRARIADKKKRIAKANSDAADYQKLL 217
Score = 70.9 bits (166), Expect = 7e-13
Identities = 37/66 (56%), Positives = 45/66 (68%), Gaps = 3/66 (4%)
Frame = +1
Query: 367 QGAQEIPGLTDGNVPRRLGPKRASKIRKLFNLSKEDDVRRYV--VKRVLQPRKEKKMLN- 537
+G ++PGLTD PR GPKRASKIRKLFNL KEDDVR+YV +R +K KK+
Sbjct: 118 KGVSDLPGLTDTEKPRMRGPKRASKIRKLFNLGKEDDVRKYVNTYRRTFTNKKGKKVSKA 177
Query: 538 PDIRHL 555
P I+ L
Sbjct: 178 PKIQRL 183
Score = 50.0 bits (114), Expect = 1e-06
Identities = 25/40 (62%), Positives = 31/40 (77%), Gaps = 2/40 (5%)
Frame = +2
Query: 257 KGHSCYRP--RRDGERKRKSVRGCIVDANLSVLALVIVRR 370
+G C+R RR GER+RKSVRGCIV +LSVL LVIV++
Sbjct: 79 RGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKK 118
>At1g24110.1 68414.m03042 peroxidase, putative similar to peroxidase
ATP26a, GB:CAA72487
Length = 326
Score = 31.9 bits (69), Expect = 0.40
Identities = 19/69 (27%), Positives = 33/69 (47%)
Frame = +1
Query: 346 LGSCYCAQGAQEIPGLTDGNVPRRLGPKRASKIRKLFNLSKEDDVRRYVVKRVLQPRKEK 525
+G +C + A I +D N P + PK A+++RKL + +D + V P K
Sbjct: 192 IGFSHCKEFASRIFNKSDQNGPVEMNPKYAAELRKLC-ANYTNDEQMSAFNDVFTPGKFD 250
Query: 526 KMLNPDIRH 552
M +++H
Sbjct: 251 NMYYKNLKH 259
>At3g24080.1 68416.m03024 KRR1 family protein contains Pfam PF05178:
Krr1 family
Length = 638
Score = 29.9 bits (64), Expect = 1.6
Identities = 13/45 (28%), Positives = 28/45 (62%)
Frame = +1
Query: 391 LTDGNVPRRLGPKRASKIRKLFNLSKEDDVRRYVVKRVLQPRKEK 525
+ +G+V ++ ++A + K + KEDDVR+ +KR+ +K++
Sbjct: 288 VVEGSVRKKDNARKAQRKNKDERMKKEDDVRKEELKRLKNVKKKE 332
>At1g21740.1 68414.m02721 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 953
Score = 29.1 bits (62), Expect = 2.8
Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = +3
Query: 60 QKLF-EVVDEHKLRIFYEKRMGAEVEADQLGDE 155
+KL+ EV DE KLR+ YE++ + D LG E
Sbjct: 602 KKLYKEVKDEEKLRVVYEEKCRTLKKLDSLGAE 634
>At1g67280.1 68414.m07657 lactoylglutathione lyase, putative /
glyoxalase I, putative similar to putative
lactoylglutathione lyase SP:Q39366, GI:2494843 from
[Brassica oleracea]
Length = 350
Score = 28.7 bits (61), Expect = 3.7
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +3
Query: 45 PATGCQKLFEVVDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVAGGNDKQGFPM 212
P CQ + V D + FYEK G E+ + E+K Y + + G + FP+
Sbjct: 216 PEPLCQVMLRVGDLDRAIKFYEKAFGMELLRTRDNPEYK-YTIAMMGYGPEDKFPV 270
>At2g16390.1 68415.m01876 SNF2 domain-containing protein / helicase
domain-containing protein low similarity to RAD54
[Drosophila melanogaster] GI:1765914; contains Pfam
profiles PF00271: Helicase conserved C-terminal domain,
PF00176: SNF2 family N-terminal domain
Length = 888
Score = 27.9 bits (59), Expect = 6.5
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +1
Query: 379 EIPGLTDGNVPRRLGPKRASKIRKL 453
E+PGL D V L PK+ ++++KL
Sbjct: 620 ELPGLADFTVVLNLSPKQLNEVKKL 644
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,292,520
Number of Sequences: 28952
Number of extensions: 302705
Number of successful extensions: 885
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 852
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 885
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1422784080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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