BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00159 (766 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4 |S... 52 7e-08 SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pom... 38 0.002 SPBC887.09c |||leucine-rich repeat protein Sog2 |Schizosaccharom... 35 0.011 SPAC926.06c |||leucine-rich repeat protein, unknown|Schizosaccha... 32 0.10 SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22... 31 0.24 SPCC24B10.22 ||SPCPB16A4.01|mitochondrial DNA polymerase gamma c... 27 2.2 SPAC212.08c |||GPI anchored protein |Schizosaccharomyces pombe|c... 26 6.8 SPAC750.07c |||S. pombe specific GPI anchored protein family 1|S... 26 6.8 SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 26 6.8 SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 25 9.0 >SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4 |Schizosaccharomyces pombe|chr 3|||Manual Length = 690 Score = 52.4 bits (120), Expect = 7e-08 Identities = 30/73 (41%), Positives = 39/73 (53%) Frame = +1 Query: 250 LISLPTEIGNLKNLVNLNLSYNPLNVLIPEIGDLEKLEAFWCNRIGLKEIPKEIGKLTQL 429 L LP EIG LKNLV L+ S N + + PE+G L +L + IP E+G L QL Sbjct: 193 LTRLPPEIGKLKNLVILDASGNSIKTIPPELGLLTELREVLLFDNMISVIPAELGTLFQL 252 Query: 430 DTFGARGNELKTI 468 G GN L+ + Sbjct: 253 KILGIEGNPLQDV 265 Score = 46.0 bits (104), Expect = 6e-06 Identities = 28/88 (31%), Positives = 46/88 (52%) Frame = +1 Query: 250 LISLPTEIGNLKNLVNLNLSYNPLNVLIPEIGDLEKLEAFWCNRIGLKEIPKEIGKLTQL 429 L ++ T++ L L +++N L L PEIG L+ L + +K IP E+G LT+L Sbjct: 170 LRNVSTDLFKFSFLTELYINHNNLTRLPPEIGKLKNLVILDASGNSIKTIPPELGLLTEL 229 Query: 430 DTFGARGNELKTIPEEMTSLAKLRWLSL 513 N + IP E+ +L +L+ L + Sbjct: 230 REVLLFDNMISVIPAELGTLFQLKILGI 257 Score = 25.4 bits (53), Expect = 9.0 Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 2/60 (3%) Frame = +2 Query: 29 FIRENHILALIEQAIAKKKSRLSLN--GFEITEIPGLLYTCLEVEHLYLHKNKISVFPVE 202 +I N++ L + I K K+ + L+ G I IP L E+ + L N ISV P E Sbjct: 187 YINHNNLTRLPPE-IGKLKNLVILDASGNSIKTIPPELGLLTELREVLLFDNMISVIPAE 245 >SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pombe|chr 2|||Manual Length = 1692 Score = 37.9 bits (84), Expect = 0.002 Identities = 19/81 (23%), Positives = 37/81 (45%) Frame = +1 Query: 271 IGNLKNLVNLNLSYNPLNVLIPEIGDLEKLEAFWCNRIGLKEIPKEIGKLTQLDTFGARG 450 I L N+ + ++YN + I ++ L+ C + + +GKL L Sbjct: 679 IETLVNVETVKVNYNHFTSISDAISAMQNLKYLSCTNCEMSYVSPNLGKLKHLVHLDLHA 738 Query: 451 NELKTIPEEMTSLAKLRWLSL 513 N +K PEE+ ++ L+ ++L Sbjct: 739 NNIKIFPEEVWQVSSLKVVNL 759 Score = 31.1 bits (67), Expect = 0.18 Identities = 19/48 (39%), Positives = 26/48 (54%), Gaps = 2/48 (4%) Frame = +1 Query: 259 LPTEIGNLKNLVNLNLSYNPLNVLIPEIGDLEKLEAF--WCNRIGLKE 396 LP EIG LKNL ++LSYN + I + + KL + CN + E Sbjct: 587 LPQEIGLLKNLETIDLSYNAI-TNIASLSECPKLNSINVACNLLSFYE 633 Score = 27.5 bits (58), Expect = 2.2 Identities = 16/58 (27%), Positives = 27/58 (46%), Gaps = 1/58 (1%) Frame = +2 Query: 32 IRENHILALIEQAIAKKKSR-LSLNGFEITEIPGLLYTCLEVEHLYLHKNKISVFPVE 202 + NH ++ + A + + LS E++ + L + HL LH N I +FP E Sbjct: 690 VNYNHFTSISDAISAMQNLKYLSCTNCEMSYVSPNLGKLKHLVHLDLHANNIKIFPEE 747 >SPBC887.09c |||leucine-rich repeat protein Sog2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 886 Score = 35.1 bits (77), Expect = 0.011 Identities = 20/75 (26%), Positives = 36/75 (48%) Frame = +1 Query: 289 LVNLNLSYNPLNVLIPEIGDLEKLEAFWCNRIGLKEIPKEIGKLTQLDTFGARGNELKTI 468 + L L +N + + PEI +L L+E P+ + +L L+ N++K + Sbjct: 54 IARLALGHNFIKSIGPEILKFTRLRYLNIRSNVLREFPESLCRLESLEILDISRNKIKQL 113 Query: 469 PEEMTSLAKLRWLSL 513 PE +L L+ LS+ Sbjct: 114 PESFGALMNLKVLSI 128 Score = 30.7 bits (66), Expect = 0.24 Identities = 14/53 (26%), Positives = 28/53 (52%) Frame = +3 Query: 495 IEMVELENNQIETLPDTMDRMTALVHCNLRNNRLEQFPSSILQCKELMFLPFE 653 +E++++ N+I+ LP++ + L ++ NRL + P+ I L L E Sbjct: 100 LEILDISRNKIKQLPESFGALMNLKVLSISKNRLFELPTYIAHMPNLEILKIE 152 Score = 30.3 bits (65), Expect = 0.31 Identities = 15/50 (30%), Positives = 29/50 (58%) Frame = +3 Query: 495 IEMVELENNQIETLPDTMDRMTALVHCNLRNNRLEQFPSSILQCKELMFL 644 I + L +N I+++ + + T L + N+R+N L +FP S+ + + L L Sbjct: 54 IARLALGHNFIKSIGPEILKFTRLRYLNIRSNVLREFPESLCRLESLEIL 103 >SPAC926.06c |||leucine-rich repeat protein, unknown|Schizosaccharomyces pombe|chr 1|||Manual Length = 621 Score = 31.9 bits (69), Expect = 0.10 Identities = 21/58 (36%), Positives = 28/58 (48%), Gaps = 1/58 (1%) Frame = +1 Query: 373 CNRIGLKEIPKEIG-KLTQLDTFGARGNELKTIPEEMTSLAKLRWLSLRIIKSKHCQT 543 C+ LK IPK + L L + GNEL IP + L +L L+L K C+T Sbjct: 339 CSSCKLKSIPKNVFLSLQSLVSLDLSGNELTEIPYALGELPQLCSLNLASNKITGCRT 396 >SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22 |Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 30.7 bits (66), Expect = 0.24 Identities = 28/81 (34%), Positives = 43/81 (53%), Gaps = 2/81 (2%) Frame = +1 Query: 271 IGNLKNLVNLNLSYNPLNVLIPEIGDLEKLE--AFWCNRIGLKEIPKEIGKLTQLDTFGA 444 + N+KNL L+LS+N + I I L+ LE F NRI E + + +LT L+ Sbjct: 100 LDNVKNLTYLDLSFNNIKT-IRNINHLKGLENLFFVQNRIRRIENLEGLDRLTNLE---L 155 Query: 445 RGNELKTIPEEMTSLAKLRWL 507 GN+++ I E + +L L L Sbjct: 156 GGNKIRVI-ENLDTLVNLEKL 175 >SPCC24B10.22 ||SPCPB16A4.01|mitochondrial DNA polymerase gamma catalytic subunit|Schizosaccharomyces pombe|chr 3|||Manual Length = 1018 Score = 27.5 bits (58), Expect = 2.2 Identities = 8/23 (34%), Positives = 16/23 (69%) Frame = +1 Query: 364 AFWCNRIGLKEIPKEIGKLTQLD 432 AF+C R+G+ E+P+ + + +D Sbjct: 915 AFFCQRLGINELPQSVAFFSSVD 937 >SPAC212.08c |||GPI anchored protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 278 Score = 25.8 bits (54), Expect = 6.8 Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 1/48 (2%) Frame = -2 Query: 708 AGYETRV*ISWY*GNLITIQMGGT-SALYIGVSMTETVLACCYVNYNG 568 +G T ++W G LI +G Y G M T++A C+ YNG Sbjct: 15 SGLATAFYVTWQ-GRLICAGVGLILEQAYEGGQMFNTLMAHCFETYNG 61 >SPAC750.07c |||S. pombe specific GPI anchored protein family 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 123 Score = 25.8 bits (54), Expect = 6.8 Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 1/48 (2%) Frame = -2 Query: 708 AGYETRV*ISWY*GNLITIQMGGT-SALYIGVSMTETVLACCYVNYNG 568 +G T ++W G LI +G Y G M T++A C+ YNG Sbjct: 15 SGLATAFYVTWQ-GRLICAGVGLILEQAYEGGQMFNTLMAHCFETYNG 61 >SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosaccharomyces pombe|chr 1|||Manual Length = 1168 Score = 25.8 bits (54), Expect = 6.8 Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = +1 Query: 376 NRIGLKEIPKEIGKLTQLDTFGARGNELKTIPE--EMTSLA 492 N I L EIG + L+TF + G + T PE E+ LA Sbjct: 306 NAIPLSAQNSEIGHVNPLETFTSNGRKRLTSPEIWELQQLA 346 >SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharomyces pombe|chr 2|||Manual Length = 733 Score = 25.4 bits (53), Expect = 9.0 Identities = 12/27 (44%), Positives = 18/27 (66%) Frame = -3 Query: 413 PISLGISFKPILLHQNASSFSRSPISG 333 P +LGI F P+LL +++ S+S SG Sbjct: 489 PEALGIIFGPLLLGNSSTDLSKSCPSG 515 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,908,410 Number of Sequences: 5004 Number of extensions: 56888 Number of successful extensions: 161 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 158 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 367316502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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