BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00156 (672 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 93 3e-20 SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 57 3e-09 SPAC144.06 |apl5||AP-3 adaptor complex subunit Apl5 |Schizosacch... 37 0.003 SPBC2G2.06c |apl1||AP-2 adaptor complex subunit Apl1 |Schizosacc... 34 0.016 SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyce... 27 1.9 SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces pom... 27 2.5 SPBP19A11.07c ||SPBP4H10.02c|human down-regulated in multiple ca... 27 3.3 SPBC27B12.11c |||transcription factor |Schizosaccharomyces pombe... 26 4.3 SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|... 26 4.3 SPBC2F12.08c |ceg1|pce1|mRNA guanylyltransferase Ceg1|Schizosacc... 26 5.7 SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat protei... 26 5.7 SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 26 5.7 SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 25 7.5 SPBC1734.03 ||SPBC337.19|dihydropteroatesynthase/2-amino-4-hydro... 25 7.5 SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb... 25 9.9 SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr... 25 9.9 >SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 878 Score = 93.1 bits (221), Expect = 3e-20 Identities = 46/89 (51%), Positives = 61/89 (68%) Frame = +2 Query: 26 EIKRINKELANIRSKFKGDKTLDGYQKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKC 205 E KR+N ELA IR+KF+ TL Y +KKYV KLL+I++LG+ I FGHMEA LLS K Sbjct: 25 EEKRVNVELAKIRAKFQSS-TLSAYDRKKYVSKLLYIYMLGYPITFGHMEAAKLLSGTKY 83 Query: 206 SEKQIGYLFISVLVNTTVTSLNLSFRALK 292 SEK IGYL +++L+N + L ++K Sbjct: 84 SEKLIGYLAVALLLNENHELMKLVINSIK 112 Score = 72.5 bits (170), Expect = 5e-14 Identities = 36/82 (43%), Positives = 55/82 (67%) Frame = +1 Query: 259 DLIKLIIQSIKNDLQSRNPIHVNLALQCIANIGSKDMAEAFGTEIPKLLVSGDTMDVVKQ 438 +L+KL+I SIK DL S + + +LAL IANIG +++ E +I KLL+S ++V+Q Sbjct: 102 ELMKLVINSIKKDLLSHDSLQNSLALHTIANIGGRELCETVYYDIYKLLMSASNENIVRQ 161 Query: 439 SAALCLLRLFRKSPEIIPGGEW 504 +AL LL ++RK P++I EW Sbjct: 162 KSALALLHIYRKFPDLI-NPEW 182 >SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schizosaccharomyces pombe|chr 3|||Manual Length = 865 Score = 56.8 bits (131), Expect = 3e-09 Identities = 34/97 (35%), Positives = 52/97 (53%) Frame = +2 Query: 2 RNCKSKEAEIKRINKELANIRSKFKGDKTLDGYQKKKYVCKLLFIFLLGHDIDFGHMEAV 181 R K+ E I KE A IR + D ++K V KLL++FLLG FG +E + Sbjct: 41 RASKTTAEEHTTILKESAQIRKNIRQGSN-DMRMRRKNVAKLLYLFLLGEPTHFGQIECL 99 Query: 182 NLLSSNKCSEKQIGYLFISVLVNTTVTSLNLSFRALK 292 LLSS++ +K++GYL +L++ L L +L+ Sbjct: 100 KLLSSSRFMDKRLGYLAAMLLLDENQEVLTLLTNSLQ 136 Score = 48.4 bits (110), Expect = 9e-07 Identities = 22/78 (28%), Positives = 44/78 (56%) Frame = +1 Query: 253 HSDLIKLIIQSIKNDLQSRNPIHVNLALQCIANIGSKDMAEAFGTEIPKLLVSGDTMDVV 432 + +++ L+ S++NDL+SR+ V LAL N+ ++A +I +L + + + Sbjct: 124 NQEVLTLLTNSLQNDLKSRDKFIVGLALSAFGNVAGPELARDLSNDIAEL--CSNHHNYI 181 Query: 433 KQSAALCLLRLFRKSPEI 486 + A LC LR+ +K P++ Sbjct: 182 SKKAVLCALRVIQKEPDL 199 >SPAC144.06 |apl5||AP-3 adaptor complex subunit Apl5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 834 Score = 36.7 bits (81), Expect = 0.003 Identities = 17/46 (36%), Positives = 28/46 (60%) Frame = +2 Query: 92 DGYQKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKCSEKQIGYL 229 D K + + KL ++ +LG DI + + V ++SS+K +KQ GYL Sbjct: 40 DADLKSEAILKLAYLEMLGVDISWASFQIVEVMSSSKILQKQKGYL 85 >SPBC2G2.06c |apl1||AP-2 adaptor complex subunit Apl1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 677 Score = 34.3 bits (75), Expect = 0.016 Identities = 21/74 (28%), Positives = 42/74 (56%) Frame = +1 Query: 268 KLIIQSIKNDLQSRNPIHVNLALQCIANIGSKDMAEAFGTEIPKLLVSGDTMDVVKQSAA 447 K ++ I ND+ S NP+ +LAL+ + ++ K+ A I +LL DT V+++AA Sbjct: 86 KRAVKLILNDIYSSNPMIRSLALRTLTSVNIKNFWVAAMDPIVRLL--DDTDPYVRKTAA 143 Query: 448 LCLLRLFRKSPEII 489 + + +L+ +++ Sbjct: 144 IGIAKLYSYDKKMV 157 >SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 610 Score = 27.5 bits (58), Expect = 1.9 Identities = 15/52 (28%), Positives = 26/52 (50%) Frame = +3 Query: 75 RVIKL*MDIKRRSMCVNFCLYFCSDMISTSGTWKQ*TYFHPINAPKNRLDTF 230 R+++L +D R+S+C FC D + W YF+ + + +LD F Sbjct: 311 RILRLVLDDSRKSVCFGFCYV---DTLKILQKWGSGCYFYGLGNDE-QLDEF 358 >SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces pombe|chr 1|||Manual Length = 986 Score = 27.1 bits (57), Expect = 2.5 Identities = 12/45 (26%), Positives = 24/45 (53%) Frame = +3 Query: 459 SLISQIS*DNTWRRVDFRIIHLLNDPHMGVVTAATSLIDALVKKN 593 SLI+ + + W V ++HLL + + A+ ++D LV ++ Sbjct: 115 SLIANVDYPDEWPEVVPAVLHLLQSTNENSINASLDVLDELVDES 159 >SPBP19A11.07c ||SPBP4H10.02c|human down-regulated in multiple cancers-1 homolog 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 676 Score = 26.6 bits (56), Expect = 3.3 Identities = 26/68 (38%), Positives = 32/68 (47%) Frame = +2 Query: 38 INKELANIRSKFKGDKTLDGYQKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKCSEKQ 217 INK L+N K + T G K+ V L F+FLL + E VN SSN E Sbjct: 326 INKFLSNPLKKERQIITFMGDFKRCVVESLCFLFLLLTNQPRFTDELVNFKSSN---EMM 382 Query: 218 IGYLFISV 241 + LFI V Sbjct: 383 MNLLFIHV 390 >SPBC27B12.11c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 738 Score = 26.2 bits (55), Expect = 4.3 Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 2/55 (3%) Frame = -1 Query: 543 PYGGHSKDELCESP--LSSRYYLRRFAK*AKEAKCSRLFHYIHGITRDKKFGYFS 385 P+ S++ +P ++ ++LR +A+ A S L H IHG+ ++ G S Sbjct: 452 PFNRKSRNRSFTNPVGMTEEHFLREYAQ-HSVANPSLLIHQIHGLPSEQVHGLLS 505 >SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 26.2 bits (55), Expect = 4.3 Identities = 9/20 (45%), Positives = 15/20 (75%) Frame = +2 Query: 338 NVLPTLVAKIWLKLLVLKYP 397 +++PT VA W+K+L +YP Sbjct: 258 DLVPTSVAAAWVKILAKEYP 277 >SPBC2F12.08c |ceg1|pce1|mRNA guanylyltransferase Ceg1|Schizosaccharomyces pombe|chr 2|||Manual Length = 402 Score = 25.8 bits (54), Expect = 5.7 Identities = 13/39 (33%), Positives = 22/39 (56%), Gaps = 3/39 (7%) Frame = -3 Query: 496 LQVLSQE---ICEISEGGKVQQIVSLHPWYHQRQEVWVF 389 LQ L ++ +CE S+G + ++ HP Y R V++F Sbjct: 54 LQALKEKNYFVCEKSDGIRCLLYMTEHPRYENRPSVYLF 92 >SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 945 Score = 25.8 bits (54), Expect = 5.7 Identities = 18/36 (50%), Positives = 21/36 (58%), Gaps = 4/36 (11%) Frame = -2 Query: 512 AKVH-SPPGIISGDLRNKRRRQS---AADCFTTSMV 417 A VH SP IISG L KRR++ A FT +MV Sbjct: 142 ASVHGSPKQIISGTLLKKRRKKGQGYARRYFTLNMV 177 >SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 25.8 bits (54), Expect = 5.7 Identities = 9/21 (42%), Positives = 15/21 (71%) Frame = +2 Query: 149 HDIDFGHMEAVNLLSSNKCSE 211 +D D+ + +NLL+SN+C E Sbjct: 1022 NDADYAILNILNLLTSNECDE 1042 >SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a Pol2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 2199 Score = 25.4 bits (53), Expect = 7.5 Identities = 18/69 (26%), Positives = 28/69 (40%), Gaps = 1/69 (1%) Frame = +2 Query: 17 KEAEIKRINKELANIRSKF-KGDKTLDGYQKKKYVCKLLFIFLLGHDIDFGHMEAVNLLS 193 + E+K I + I F KGD + YQ+ YV L + E + L+S Sbjct: 973 RRGELKLIKDFQSQIFKVFLKGDSLEECYQEVAYVADTWLEILFTKGSNLTDDELIELIS 1032 Query: 194 SNKCSEKQI 220 N+ K + Sbjct: 1033 ENRSMSKAL 1041 >SPBC1734.03 ||SPBC337.19|dihydropteroatesynthase/2-amino-4-hydroxy- 6- hydroxymethyldihydropteridinediphosphokinase/dihydroneop terinaldolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 25.4 bits (53), Expect = 7.5 Identities = 15/45 (33%), Positives = 21/45 (46%) Frame = -1 Query: 630 LQPMLHNPYTHQDSSLQEHQSKMSLQLLHPYGGHSKDELCESPLS 496 L P L +PYTHQ LQE K+ Q + Y ++ L+ Sbjct: 378 LSPDLVHPYTHQ--PLQEALDKLPSQGIRLYSSFDNKKIINGALT 420 >SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pombe|chr 3|||Manual Length = 1254 Score = 25.0 bits (52), Expect = 9.9 Identities = 13/47 (27%), Positives = 23/47 (48%), Gaps = 2/47 (4%) Frame = -3 Query: 511 RKSTLLQVLSQEICEISEGGKVQQIVSLHPWYHQRQEVW--VFQYQK 377 R ++ L +E E++EG + +I+ P + E W F +QK Sbjct: 777 RINSAFDQLREEFMEVAEGTLLGEIIQSAPNMKEELEAWTRAFNWQK 823 >SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr 2|||Manual Length = 1250 Score = 25.0 bits (52), Expect = 9.9 Identities = 11/32 (34%), Positives = 16/32 (50%) Frame = +1 Query: 310 NPIHVNLALQCIANIGSKDMAEAFGTEIPKLL 405 NP+H N L + D+A F + IPK + Sbjct: 164 NPLHENYCLNSLRKDQYVDLAIQFNSTIPKYI 195 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,722,371 Number of Sequences: 5004 Number of extensions: 57180 Number of successful extensions: 193 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 181 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 191 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 307866294 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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