BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00150 (747 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr... 32 0.076 SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|ch... 30 0.30 SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom... 29 0.53 SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo... 27 2.1 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 27 2.8 SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce... 27 3.8 SPBC146.03c |cut3|smc4, smc4|condensin subunit Cut3|Schizosaccha... 26 5.0 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 26 5.0 SPAC1783.01 |||FAD binding protein|Schizosaccharomyces pombe|chr... 26 6.6 SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharo... 26 6.6 SPAC23G3.04 |||DUF1711 family protein|Schizosaccharomyces pombe|... 25 8.7 SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1 |... 25 8.7 >SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr 3|||Manual Length = 1516 Score = 32.3 bits (70), Expect = 0.076 Identities = 28/100 (28%), Positives = 51/100 (51%), Gaps = 1/100 (1%) Frame = +1 Query: 310 AKLSEASQAADESERIRKALENRTNMEDDRVAILEAQLSQANSSPRSRTRNTKRWRATCS 489 ++L E S+ D SE+ EN N +R+A LE+ LS + ++ R ++ R S Sbjct: 930 SRLFEISKQLDNSEQ-----EN--NKFRERIAELESHLSNYAEAKLAQERELEQTRVLIS 982 Query: 490 YGAGPRGAEERAEQSDCKIVELEEELRVVGN-NLKSLEVS 606 + +E E+ + ++ +EEE+R V + N + L V+ Sbjct: 983 DQSQDGELKELLEEKENALIMMEEEMRQVNDANTELLRVN 1022 >SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 30.3 bits (65), Expect = 0.30 Identities = 30/147 (20%), Positives = 55/147 (37%) Frame = +1 Query: 304 ATAKLSEASQAADESERIRKALENRTNMEDDRVAILEAQLSQANSSPRSRTRNTKRWRAT 483 A A+ EA A+ +E K +E + ++++ L + A S TK+ R Sbjct: 11 ARAETDEAVARAEAAEAKLKEVELQLSLKEQEYESLSRKSEAAESQLEELEEETKQLRLK 70 Query: 484 CSYGAGPRGAEERAEQSDCKIVELEEELRVVGNNLKSLEVSEEKATKREETYETHLKLLD 663 + AEQ K+ LEEEL L+ + + E +E ++ L+ Sbjct: 71 AD---NEDIQKTEAEQLSRKVELLEEELETNDKLLRETTEKMRQTDVKAEHFERRVQSLE 127 Query: 664 AQLKXXXXXXXXXXXSVQKLQKEVDRL 744 + K++ E+D + Sbjct: 128 RERDDMEQKLEEMTDKYTKVKAELDEV 154 >SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 29.5 bits (63), Expect = 0.53 Identities = 27/123 (21%), Positives = 51/123 (41%), Gaps = 4/123 (3%) Frame = +1 Query: 307 TAKLSEASQAADESERIRKALENRTNMEDDRVAILEAQ--LSQANSSPRSRTRNTKRWRA 480 TA L E D E ++ L+N++ + AIL + L + S P+ + ++ + Sbjct: 261 TASLEEYLNDPDFEENRKQYLQNKSGSPVEETAILNRKPTLRKKKSIPKKQNESSSTIQK 320 Query: 481 TCSYGAGPRGAEERAEQSDCKIVELEEELRVVGNNLKSLEVSEEKATKREE--TYETHLK 654 + +EE A +S + + +K E+ E+ + EE YE+ + Sbjct: 321 ENTVQQEASSSEEEAVKSLPETQRTTSRIETQEEEIKEEEMEGEEEEEEEEVPNYESENE 380 Query: 655 LLD 663 L D Sbjct: 381 LED 383 >SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 27.5 bits (58), Expect = 2.1 Identities = 35/154 (22%), Positives = 61/154 (39%), Gaps = 11/154 (7%) Frame = +1 Query: 304 ATAKLSEASQAADESERIRKALENRTNMEDDRVAILEAQLSQ---ANSSPRSRTRNTKRW 474 AT K+ A E+ + E + + + A E++++ NSS Sbjct: 27 ATEKVDRALLLQRRKEKAKARAEAKKLAKKESKAKQESKVAAYDTGNSSDNIADEENDNH 86 Query: 475 RATCSYGAGPRGAEERAEQSDCKIVELEEELRVVGNNLKSLE--------VSEEKATKRE 630 ++T +YG G +++ K+ + V LK LE + EEK K E Sbjct: 87 KSTITYGTLIVG-DDKFSNGKLKVAGKKRGPTDVFGALKHLEAKKRRIESMDEEKRRKIE 145 Query: 631 ETYETHLKLLDAQLKXXXXXXXXXXXSVQKLQKE 732 E+ + H LL A+ K S+++ +KE Sbjct: 146 ESDKWHRVLLQAEGKKLKDNEQLLKKSIRRKEKE 179 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 27.1 bits (57), Expect = 2.8 Identities = 17/51 (33%), Positives = 24/51 (47%) Frame = +1 Query: 316 LSEASQAADESERIRKALENRTNMEDDRVAILEAQLSQANSSPRSRTRNTK 468 LSE AA R + LEN T +E L A L ++ S +S + +K Sbjct: 181 LSERKSAAKPVGRTVEKLENATKVEKSAPEELFASLKKSASQKKSAAKESK 231 >SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 993 Score = 26.6 bits (56), Expect = 3.8 Identities = 14/38 (36%), Positives = 17/38 (44%) Frame = -2 Query: 194 ATVCGTPRTRPYIFPFPLSFDAFSSSKRLKSFLAACLS 81 A+V GTP RP F FP S K+ A +S Sbjct: 771 ASVDGTPMVRPLFFEFPKQISLASVDKQFMIGTALLIS 808 >SPBC146.03c |cut3|smc4, smc4|condensin subunit Cut3|Schizosaccharomyces pombe|chr 2|||Manual Length = 1324 Score = 26.2 bits (55), Expect = 5.0 Identities = 32/148 (21%), Positives = 59/148 (39%), Gaps = 11/148 (7%) Frame = +1 Query: 337 ADESERIRKALENRTNMEDDRVAIL-----EAQL-SQANSSPRS---RTRNTKRWRATC- 486 A++ R++ L + +ED + ++L E +L + N R+ TRN K Sbjct: 320 AEKESRLKLVLSEKAKLEDSKNSVLSFLKDENELFMKQNQLYRTILYETRNKKTLVQNLL 379 Query: 487 -SYGAGPRGAEERAEQSDCKIVELEEELRVVGNNLKSLEVSEEKATKREETYETHLKLLD 663 S + E+ EQ++ I E EE++ + ++ K ++YE ++ Sbjct: 380 NSLEGKLQAHLEKFEQTERDISEKNEEVKSLREKAAKVKNDCTSEKKTRQSYEQQTVKIE 439 Query: 664 AQLKXXXXXXXXXXXSVQKLQKEVDRLE 747 QLK S++ L E E Sbjct: 440 EQLKFLLNKEKKLKKSIEALSFEKSEAE 467 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 26.2 bits (55), Expect = 5.0 Identities = 20/67 (29%), Positives = 30/67 (44%), Gaps = 2/67 (2%) Frame = +1 Query: 553 LEEELRVVGNNLKSLEVSEEKATKREETYETHLKL--LDAQLKXXXXXXXXXXXSVQKLQ 726 LE+E + + LKSLE EK E E H++L L +LK + Q Sbjct: 897 LEQESAQLNSGLKSLEA--EKQLLHTENEELHIRLDKLTGKLKIEESKSSDLGKKLTARQ 954 Query: 727 KEVDRLE 747 +E+ L+ Sbjct: 955 EEISNLK 961 >SPAC1783.01 |||FAD binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 583 Score = 25.8 bits (54), Expect = 6.6 Identities = 13/41 (31%), Positives = 20/41 (48%) Frame = -3 Query: 451 DSSAMSSPVTVEPPRWQRDHPPCWCGSPAPCVFARIHRRPG 329 D+ SP + PP + + HPP + AP + R+ PG Sbjct: 140 DTVISDSPTSPHPPSFVQPHPPYGIFA-APILDVRVLTNPG 179 >SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharomyces pombe|chr 1|||Manual Length = 461 Score = 25.8 bits (54), Expect = 6.6 Identities = 11/28 (39%), Positives = 18/28 (64%) Frame = +1 Query: 592 SLEVSEEKATKREETYETHLKLLDAQLK 675 ++E EE + + ETYE + L+AQL+ Sbjct: 211 TIECLEESSNRAIETYENRIAELEAQLE 238 >SPAC23G3.04 |||DUF1711 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 194 Score = 25.4 bits (53), Expect = 8.7 Identities = 12/45 (26%), Positives = 22/45 (48%) Frame = +1 Query: 433 NSSPRSRTRNTKRWRATCSYGAGPRGAEERAEQSDCKIVELEEEL 567 +S P+ + R + + YG GPR + R + K+ + +EL Sbjct: 34 SSQPKPQERPFQMKKPRAPYGMGPRAMKRREKAEKEKLGVVNDEL 78 >SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 550 Score = 25.4 bits (53), Expect = 8.7 Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 1/49 (2%) Frame = +1 Query: 526 EQSDCKIVELEEELRVVGNNLKSLEVSEEKATKREETYETHL-KLLDAQ 669 + ++ + V E+ R G N L ++ K+++ YE H+ KLLD + Sbjct: 228 DAAEIEEVSAEKARRQFGKNGIRLSQDAQEEEKQKKKYEQHIKKLLDGE 276 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,406,664 Number of Sequences: 5004 Number of extensions: 41141 Number of successful extensions: 163 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 156 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 163 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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