BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00144 (734 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 124 1e-29 SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 121 9e-29 SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 45 1e-05 SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe... 34 0.018 SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 30 0.39 SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr 3... 28 1.6 SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr... 27 2.8 SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein Utp5|Schiz... 25 8.5 >SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 124 bits (300), Expect = 1e-29 Identities = 51/92 (55%), Positives = 70/92 (76%) Frame = +1 Query: 256 VTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQELYH 435 V +V+PGDHV+ LY P+C CKFC + KTNLC K+R TQG+G+MPDGT RF C+ + L H Sbjct: 82 VINVRPGDHVILLYTPECKECKFCRSGKTNLCSKIRETQGRGLMPDGTSRFSCRDKTLLH 141 Query: 436 FMGCSTFSQYTVVLEISLCKVAEAVH*IKFVC 531 +MGCS+FSQYTVV +ISL ++ + ++ +C Sbjct: 142 YMGCSSFSQYTVVADISLVAISHSAP-LRSIC 172 Score = 106 bits (254), Expect = 4e-24 Identities = 49/69 (71%), Positives = 55/69 (79%) Frame = +2 Query: 14 MSTVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPE 193 MS GK I C AAVAW A +PLSIE+I+V PPKA EVRVK+ + VCHTDAYTLSG DPE Sbjct: 1 MSFEGKTITCKAAVAWGAKEPLSIEDIQVAPPKAHEVRVKVDWSAVCHTDAYTLSGVDPE 60 Query: 194 GVFPVVLGH 220 G FP+VLGH Sbjct: 61 GAFPIVLGH 69 Score = 67.3 bits (157), Expect = 2e-12 Identities = 33/75 (44%), Positives = 41/75 (54%) Frame = +3 Query: 510 PLDKVCLLGCGVPTGYGAALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIGVDI 689 PL +CLLGCGV TG+GA ++AKVE GS A+ +RII +DI Sbjct: 167 PLRSICLLGCGVTTGFGAVTHSAKVESGSTVAVVGCGCVGLAAMQGAVAAGASRIIAIDI 226 Query: 690 NPDKFEVAKKFGVNE 734 N DK AKKFG + Sbjct: 227 NADKEVYAKKFGATD 241 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 121 bits (292), Expect = 9e-29 Identities = 50/81 (61%), Positives = 64/81 (79%) Frame = +1 Query: 256 VTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQELYH 435 VT+V+ GD V+ LY P+C TCKFC + KTNLC ++R+TQG+G+MPDGT RF C G L H Sbjct: 85 VTTVQVGDPVIALYTPECKTCKFCKSGKTNLCGRIRTTQGKGLMPDGTSRFSCNGNTLLH 144 Query: 436 FMGCSTFSQYTVVLEISLCKV 498 FMGCSTFS+YTVV +IS+ + Sbjct: 145 FMGCSTFSEYTVVADISVVAI 165 Score = 109 bits (262), Expect = 4e-25 Identities = 46/67 (68%), Positives = 55/67 (82%) Frame = +2 Query: 20 TVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGV 199 T GK+I C AAVAW+ PLSIE ++V PP+ EVR+KI +GVCHTDAYTLSGKDPEG+ Sbjct: 6 TAGKIINCKAAVAWQPAAPLSIENVQVFPPRVHEVRIKIVNSGVCHTDAYTLSGKDPEGL 65 Query: 200 FPVVLGH 220 FPV+LGH Sbjct: 66 FPVILGH 72 Score = 68.5 bits (160), Expect = 9e-13 Identities = 32/78 (41%), Positives = 41/78 (52%) Frame = +3 Query: 501 RGCPLDKVCLLGCGVPTGYGAALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIG 680 R PLD VCLLGCG+ TGYGAA TA ++ G + A+F RI G Sbjct: 167 RLAPLDSVCLLGCGITTGYGAATITADIKEGDSVAVFGLGSVGLAVIQGAVKKRAGRIFG 226 Query: 681 VDINPDKFEVAKKFGVNE 734 +D+NP+K A FG + Sbjct: 227 IDVNPEKKNWAMSFGATD 244 >SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharomyces pombe|chr 3|||Manual Length = 350 Score = 44.8 bits (101), Expect = 1e-05 Identities = 28/64 (43%), Positives = 34/64 (53%), Gaps = 3/64 (4%) Frame = +2 Query: 38 KCLAAVAWEAGKPLSI--EEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDP-EGVFPV 208 K LAAV G P ++ EE+ V P EV V I TGVCHTD + L G P P+ Sbjct: 6 KQLAAVFHTHGGPENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQGDWPLPAKMPL 65 Query: 209 VLGH 220 + GH Sbjct: 66 IGGH 69 >SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 325 Score = 34.3 bits (75), Expect = 0.018 Identities = 15/47 (31%), Positives = 26/47 (55%) Frame = +2 Query: 77 LSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGVFPVVLG 217 + I+ + + PK GE+ VKI A + +D +G P V+P ++G Sbjct: 23 IEIQSVPIPQPKNGELLVKIEAAAINPSDLMNATGGFPYTVYPRIVG 69 >SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 360 Score = 29.9 bits (64), Expect = 0.39 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = +1 Query: 256 VTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLC 351 V+S+KPGD V C C +C + + NLC Sbjct: 80 VSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLC 111 >SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 260 Score = 27.9 bits (59), Expect = 1.6 Identities = 8/21 (38%), Positives = 16/21 (76%) Frame = +3 Query: 669 RIIGVDINPDKFEVAKKFGVN 731 R++G+D +PD + A++ G+N Sbjct: 57 RVVGIDASPDMIKAARELGLN 77 >SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr 2|||Manual Length = 1250 Score = 27.1 bits (57), Expect = 2.8 Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 1/53 (1%) Frame = +1 Query: 274 GDHVVPLY-VPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQEL 429 G HVV L V N C + KT KV + + P G+R++ C G L Sbjct: 1036 GKHVVRLLQVRDANGCAASIT-KTQPAAKVSVVEMASLAPLGSRQYYCVGDRL 1087 >SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein Utp5|Schizosaccharomyces pombe|chr 1|||Manual Length = 666 Score = 25.4 bits (53), Expect = 8.5 Identities = 15/38 (39%), Positives = 23/38 (60%), Gaps = 1/38 (2%) Frame = +2 Query: 38 KCLAAVAWEAGKPL-SIEEIEVDPPKAGEVRVKITATG 148 K ++ +AWE KPL + E+I D +GE+ V T +G Sbjct: 63 KVISCIAWEQ-KPLYASEQITTDISGSGEILVLGTNSG 99 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,841,749 Number of Sequences: 5004 Number of extensions: 55571 Number of successful extensions: 149 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 147 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 347244562 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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