Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00135
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC613.08 |||CDK regulator |Schizosaccharomyces pombe|chr 3|||M... 40 3e-04
SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor eIF... 31 0.21
SPCC663.05c |cia1||histone chaperone Cia1|Schizosaccharomyces po... 30 0.37
SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac... 30 0.37
SPAC1071.11 |||NADH-dependent flavin oxidoreductase |Schizosacch... 29 0.85
SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa... 28 1.1
SPAC1486.09 |||ribosome biogenesis protein Nob1 |Schizosaccharom... 28 1.5
SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces p... 27 2.6
SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccha... 27 2.6
SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|... 27 3.4
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 26 4.5
SPAC6B12.13 |||protein phosphatase inhibitor |Schizosaccharomyce... 26 6.0
SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit |... 26 6.0
SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|S... 26 6.0
SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces ... 25 7.9
SPAC1071.08 |rpp203|rpp2-3, rla6|60S acidic ribosomal protein P2... 25 7.9
>SPCC613.08 |||CDK regulator |Schizosaccharomyces pombe|chr
3|||Manual
Length = 325
Score = 40.3 bits (90), Expect = 3e-04
Identities = 20/45 (44%), Positives = 29/45 (64%), Gaps = 2/45 (4%)
Frame = +1
Query: 124 LQADFEGRNPEDSDFHGIKQLLRQL--FLKSNVDLGGLAQIIISQ 252
+ DFE +P+ DFH K LL+QL + +NV+L LA +I+SQ
Sbjct: 69 INVDFEFFDPQPIDFHAFKNLLKQLLGYDHTNVNLSALADLILSQ 113
Score = 39.5 bits (88), Expect = 5e-04
Identities = 17/48 (35%), Positives = 30/48 (62%)
Frame = +2
Query: 509 RILNIPAAISVPLFASLQTELEKAHRKNMLYNFKYLIWISKTYTTGES 652
R++N+P + P++ L E++ A +N YNF + + +S+TYT ES
Sbjct: 188 RLINMPVQVIPPMYNMLLEEMQWAINENEPYNFTHYLLLSRTYTEIES 235
Score = 33.5 bits (73), Expect = 0.030
Identities = 15/59 (25%), Positives = 35/59 (59%), Gaps = 1/59 (1%)
Frame = +3
Query: 336 FGVTTVVNITKRKNEPSVAQIRE-LLTKLSQENADPRTKELIKYILADDSQHTGLVINE 509
+ + +V+N+ R++EP + Q+ ++++L++ N+ EL K + + GL++NE
Sbjct: 130 YAMLSVINLNTRRDEPVIKQLTSYIISRLAKSNS-RLENELQKLLEPNSGSQVGLIVNE 187
>SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor
eIF3c|Schizosaccharomyces pombe|chr 1|||Manual
Length = 918
Score = 30.7 bits (66), Expect = 0.21
Identities = 16/60 (26%), Positives = 37/60 (61%), Gaps = 5/60 (8%)
Frame = +1
Query: 1 KMPNKK----NKEIDTNSERTKEQD-SGSEKDSDFDSDGNYVGEKELQADFEGRNPEDSD 165
++P KK +++ +++SE ++E++ + SE+DS+ + E E +++ E + E+SD
Sbjct: 68 EVPKKKAVAASEDSESDSESSEEEEETESEEDSEVSDESESESESESESEEESESEEESD 127
>SPCC663.05c |cia1||histone chaperone Cia1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 262
Score = 29.9 bits (64), Expect = 0.37
Identities = 12/49 (24%), Positives = 25/49 (51%)
Frame = +1
Query: 28 IDTNSERTKEQDSGSEKDSDFDSDGNYVGEKELQADFEGRNPEDSDFHG 174
+ +++ +E++ E + +FD +G E+E + D +G D D G
Sbjct: 170 VQPDADEEEEEEEADEMEEEFDEEGEGDEEEEEEDDGDGDGEGDGDGEG 218
Score = 26.2 bits (55), Expect = 4.5
Identities = 12/49 (24%), Positives = 23/49 (46%)
Frame = +1
Query: 10 NKKNKEIDTNSERTKEQDSGSEKDSDFDSDGNYVGEKELQADFEGRNPE 156
++ +E D E +E++ + D D + DG+ GE + + E E
Sbjct: 184 DEMEEEFDEEGEGDEEEEEEDDGDGDGEGDGDGEGENDGKGSEEEEEEE 232
>SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 848
Score = 29.9 bits (64), Expect = 0.37
Identities = 25/68 (36%), Positives = 42/68 (61%), Gaps = 7/68 (10%)
Frame = +1
Query: 25 EIDTNSERTKEQDSGSEKDSDFDSDGNYVGEKELQAD--FEGR--NPEDSDFH--GIKQ- 183
+I T++++ K ++ G E SD D DGNY+ K L+++ +G+ N + S+F G+ Q
Sbjct: 22 DIATDNQKDKHENVG-ENVSDED-DGNYIASKLLESNRRTKGKKGNGKASNFQSMGLNQT 79
Query: 184 LLRQLFLK 207
LLR +F K
Sbjct: 80 LLRAIFKK 87
>SPAC1071.11 |||NADH-dependent flavin oxidoreductase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 244
Score = 28.7 bits (61), Expect = 0.85
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = -1
Query: 175 YRGSHYLLGYVLRNQPVALFHRHSCRLNQNQNLFRCL 65
+ S L Y+LR+ P + F+R+S + N N FR L
Sbjct: 9 FTSSSSLPCYILRHLPFSNFNRYSTASHDNVNAFRLL 45
>SPAC23C4.19 |spt5||transcription elongation factor
Spt5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 990
Score = 28.3 bits (60), Expect = 1.1
Identities = 13/56 (23%), Positives = 29/56 (51%)
Frame = +1
Query: 25 EIDTNSERTKEQDSGSEKDSDFDSDGNYVGEKELQADFEGRNPEDSDFHGIKQLLR 192
E+D + K++D + ++++ ++D N E++ + D E ED G ++ R
Sbjct: 84 EVDNVNNNDKKEDEDNVEENEEEADANEEEEEDEEDDEEDEEDEDESGGGRRKRAR 139
>SPAC1486.09 |||ribosome biogenesis protein Nob1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 388
Score = 27.9 bits (59), Expect = 1.5
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +1
Query: 10 NKKNKEIDTNSERTKEQDSGSEKDSD 87
+ NKE N+E KEQ+ E D D
Sbjct: 163 HSNNKEAHPNTEENKEQEDNEEDDED 188
>SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 773
Score = 27.1 bits (57), Expect = 2.6
Identities = 23/85 (27%), Positives = 39/85 (45%)
Frame = +1
Query: 1 KMPNKKNKEIDTNSERTKEQDSGSEKDSDFDSDGNYVGEKELQADFEGRNPEDSDFHGIK 180
K+P +K+ S+R++ SGS D++G G+K + +G N D K
Sbjct: 74 KIPLISSKQFSVTSKRSQNGSSGSNS----DANGRKNGQKNDDSKKKGLNGNDP-----K 124
Query: 181 QLLRQLFLKSNVDLGGLAQIIISQN 255
++ ++ L N LGG+ I N
Sbjct: 125 KVF-EIALNGNTILGGILVAYILYN 148
>SPBC660.15 |||mRNA cleavage factor complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 474
Score = 27.1 bits (57), Expect = 2.6
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +1
Query: 22 KEIDTNSERTKEQDSGSEKDSDFDSDGNYVGEKELQADFEGRNPED 159
K + +ERTKE E +DFD G+ +++ A+ E +D
Sbjct: 50 KSGEAGAERTKENPFREEPGADFDRSGS---DQQYSAEAEANQEDD 92
>SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit
Alp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 784
Score = 26.6 bits (56), Expect = 3.4
Identities = 16/58 (27%), Positives = 33/58 (56%), Gaps = 2/58 (3%)
Frame = +3
Query: 342 VTTVVNITKRKNEPSVA-QIRELLTK-LSQENADPRTKELIKYILADDSQHTGLVINE 509
++ VVN T+ K + Q+ LT+ L++ DP ++++ Y+L + S+ ++NE
Sbjct: 239 ISHVVNATRSKKKVCKGGQVISFLTESLTKYAGDPVARKILTYLLREASRPYTKMLNE 296
>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
Gap1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 766
Score = 26.2 bits (55), Expect = 4.5
Identities = 10/28 (35%), Positives = 19/28 (67%), Gaps = 1/28 (3%)
Frame = -1
Query: 412 VSNSLICATLGSFFLL-VIFTTVVTPKT 332
+S+S IC+ +G FF L + +++P+T
Sbjct: 324 ISDSTICSLIGGFFFLRFVNPAIISPQT 351
>SPAC6B12.13 |||protein phosphatase inhibitor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 104
Score = 25.8 bits (54), Expect = 6.0
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +1
Query: 4 MPNKKNKEIDTNSERTKEQDSGSEKDSDFDSDGN 105
M KK+K ++ K +S S+ DSD DSD +
Sbjct: 58 MNKKKSKVCCIFHKQRKFDESSSDSDSDSDSDSS 91
>SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 661
Score = 25.8 bits (54), Expect = 6.0
Identities = 13/47 (27%), Positives = 22/47 (46%)
Frame = +3
Query: 348 TVVNITKRKNEPSVAQIRELLTKLSQENADPRTKELIKYILADDSQH 488
T N+ N PSV + ++ NAD + E IK ++ +S +
Sbjct: 358 TADNMNTWDNTPSVENVESANENVNNHNADEQMDEKIKSLVEGNSAY 404
>SPAC3H1.04c |mdm31||mitochondrial inner membrane protein
Mdm31|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 25.8 bits (54), Expect = 6.0
Identities = 20/67 (29%), Positives = 30/67 (44%)
Frame = +1
Query: 7 PNKKNKEIDTNSERTKEQDSGSEKDSDFDSDGNYVGEKELQADFEGRNPEDSDFHGIKQL 186
PN K+ K DS E D++ S GNY + EL D + + F K +
Sbjct: 224 PNNKSDYEKEYMALRKRYDSNEEPDTEALSQGNYT-QFELSIDKADVSFSFARFLNGKGI 282
Query: 187 LRQLFLK 207
+++L LK
Sbjct: 283 VKELQLK 289
>SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 25.4 bits (53), Expect = 7.9
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = +1
Query: 16 KNKEIDTNSERTKEQDSGSEKDSDFDSDGNYVGEKELQADFEGRNPEDSD 165
KNK T+ + T ++ G EKD ++ + G+ + + +N DSD
Sbjct: 227 KNKSTPTDEDVTFTEEDGDEKDEEYSDIFSIKGKFNTRPN-AWQNAYDSD 275
>SPAC1071.08 |rpp203|rpp2-3, rla6|60S acidic ribosomal protein P2C
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 110
Score = 25.4 bits (53), Expect = 7.9
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +3
Query: 372 KNEPSVAQIRELLTKLSQENADPRTKELIKYILADD 479
KN PS + I +L+ + E+ R + LIK + D
Sbjct: 15 KNSPSASDIESVLSTVGIESESERVEALIKELDGKD 50
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,513,945
Number of Sequences: 5004
Number of extensions: 47644
Number of successful extensions: 209
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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