BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00134 (723 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 180 1e-47 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 176 2e-46 AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 166 2e-43 EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 143 1e-36 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003 AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.3 AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.9 D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.9 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 8.9 AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 8.9 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 180 bits (438), Expect = 1e-47 Identities = 85/86 (98%), Positives = 85/86 (98%) Frame = +2 Query: 251 G*GSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 430 G GSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 109 Query: 431 DCAVLIVAAGTGEFEAGISKNGQTRE 508 DCAVLIVAAGTGEFEAGISKNGQTRE Sbjct: 110 DCAVLIVAAGTGEFEAGISKNGQTRE 135 Score = 143 bits (347), Expect = 1e-36 Identities = 68/71 (95%), Positives = 69/71 (97%) Frame = +1 Query: 511 ALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 690 ALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGW Sbjct: 137 ALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 196 Query: 691 HGDNMLEPSTK 723 HGDNMLE S+K Sbjct: 197 HGDNMLEVSSK 207 Score = 109 bits (262), Expect = 3e-26 Identities = 50/51 (98%), Positives = 50/51 (98%) Frame = +3 Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 51 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 176 bits (429), Expect = 2e-46 Identities = 83/86 (96%), Positives = 84/86 (97%) Frame = +2 Query: 251 G*GSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 430 G GSFKYAWVLDKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQA Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQA 109 Query: 431 DCAVLIVAAGTGEFEAGISKNGQTRE 508 DCAVLIVAAG GEFEAGISKNGQTRE Sbjct: 110 DCAVLIVAAGIGEFEAGISKNGQTRE 135 Score = 138 bits (335), Expect = 4e-35 Identities = 65/71 (91%), Positives = 67/71 (94%) Frame = +1 Query: 511 ALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 690 ALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGW Sbjct: 137 ALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGW 196 Query: 691 HGDNMLEPSTK 723 HGDNMLEPS K Sbjct: 197 HGDNMLEPSPK 207 Score = 109 bits (262), Expect = 3e-26 Identities = 50/51 (98%), Positives = 50/51 (98%) Frame = +3 Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 51 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 166 bits (404), Expect = 2e-43 Identities = 78/78 (100%), Positives = 78/78 (100%) Frame = +2 Query: 275 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 454 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60 Query: 455 AGTGEFEAGISKNGQTRE 508 AGTGEFEAGISKNGQTRE Sbjct: 61 AGTGEFEAGISKNGQTRE 78 Score = 143 bits (347), Expect = 1e-36 Identities = 68/71 (95%), Positives = 69/71 (97%) Frame = +1 Query: 511 ALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 690 ALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGW Sbjct: 80 ALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 139 Query: 691 HGDNMLEPSTK 723 HGDNMLE S+K Sbjct: 140 HGDNMLEVSSK 150 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 143 bits (347), Expect = 1e-36 Identities = 68/71 (95%), Positives = 69/71 (97%) Frame = +1 Query: 511 ALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 690 ALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGW Sbjct: 64 ALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 123 Query: 691 HGDNMLEPSTK 723 HGDNMLE S+K Sbjct: 124 HGDNMLEVSSK 134 Score = 133 bits (322), Expect = 1e-33 Identities = 62/62 (100%), Positives = 62/62 (100%) Frame = +2 Query: 323 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 502 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60 Query: 503 RE 508 RE Sbjct: 61 RE 62 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 33.1 bits (72), Expect = 0.003 Identities = 16/35 (45%), Positives = 18/35 (51%) Frame = +2 Query: 362 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 466 VT +D PGH FI G D VL+VAA G Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229 Score = 25.4 bits (53), Expect = 0.55 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +3 Query: 117 KTHINIVVIGHVDSGKST 170 K H + ++GHVD GK+T Sbjct: 143 KRHPIVTIMGHVDHGKTT 160 >AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methyltransferase protein. Length = 683 Score = 24.2 bits (50), Expect = 1.3 Identities = 11/15 (73%), Positives = 11/15 (73%) Frame = +3 Query: 126 INIVVIGHVDSGKST 170 INI IGHV GKST Sbjct: 43 INIGTIGHVAHGKST 57 >AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 23.0 bits (47), Expect = 2.9 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +2 Query: 347 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 448 T KYY D P + FIKN+ ++ +D LI Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327 >D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 22.6 bits (46), Expect = 3.9 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +2 Query: 347 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 448 T KYY D P + FIKN+ ++ +D L+ Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 21.4 bits (43), Expect = 8.9 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = -1 Query: 723 FG*RLQHVVSVPSRNGHESDSSWV 652 FG L H++ V +N + + WV Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58 >AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase protein. Length = 580 Score = 21.4 bits (43), Expect = 8.9 Identities = 7/20 (35%), Positives = 13/20 (65%) Frame = +2 Query: 566 WIPLNHHTVSPDLRKSRRKY 625 W+P+N + S +L +R+Y Sbjct: 443 WLPVNENYKSLNLAAQKREY 462 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 212,611 Number of Sequences: 438 Number of extensions: 4268 Number of successful extensions: 23 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 16 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 23 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 22413960 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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