BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00131 (705 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal pro... 143 2e-35 SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein S4|Schizosacch... 143 2e-35 SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein S4|Schizos... 142 4e-35 SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po... 29 0.86 SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 27 2.0 SPAC806.06c |||nicotinamide mononucleotide |Schizosaccharomyces ... 27 2.0 SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 27 3.5 SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces p... 26 4.6 SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 26 6.0 SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce... 25 8.0 SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 25 8.0 SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyce... 25 8.0 SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pom... 25 8.0 SPBC18H10.10c |cwc16||complexed with Cdc5 protein Cwf16|Schizosa... 25 8.0 >SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal protein S4|Schizosaccharomyces pombe|chr 2|||Manual Length = 262 Score = 143 bits (347), Expect = 2e-35 Identities = 64/84 (76%), Positives = 73/84 (86%) Frame = +1 Query: 256 IEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPD 435 +EKT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKRV G K VP+LVTHDGRTIRYPD Sbjct: 92 VEKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKRVQLGAKGVPFLVTHDGRTIRYPD 151 Query: 436 PLIKVNDSIQLDIATTKIMDFIKF 507 PLIKVND+I+L++ T KI FIKF Sbjct: 152 PLIKVNDTIKLNLETNKIESFIKF 175 Score = 141 bits (341), Expect = 1e-34 Identities = 62/84 (73%), Positives = 70/84 (83%) Frame = +2 Query: 2 KHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVK 181 KHLKR+ AP W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G EV I+ Sbjct: 7 KHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGREVKAILM 66 Query: 182 QRLIKVDGKVRTDPTYPAGFMDVV 253 QRLIKVDGKVRTD T+P GFMDV+ Sbjct: 67 QRLIKVDGKVRTDSTFPTGFMDVI 90 Score = 79.4 bits (187), Expect = 5e-16 Identities = 34/52 (65%), Positives = 43/52 (82%) Frame = +3 Query: 525 MITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKGTK 680 M+TGGRN+GRVGTIV RE H GSF+I+H+KD+ FATRL+NVF+IG+ K Sbjct: 182 MVTGGRNMGRVGTIVHREHHLGSFEIIHVKDALDREFATRLSNVFVIGETGK 233 >SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein S4|Schizosaccharomyces pombe|chr 2|||Manual Length = 262 Score = 143 bits (347), Expect = 2e-35 Identities = 64/84 (76%), Positives = 73/84 (86%) Frame = +1 Query: 256 IEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPD 435 +EKT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKRV G K VP+LVTHDGRTIRYPD Sbjct: 92 VEKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKRVQLGAKGVPFLVTHDGRTIRYPD 151 Query: 436 PLIKVNDSIQLDIATTKIMDFIKF 507 PLIKVND+I+L++ T KI FIKF Sbjct: 152 PLIKVNDTIKLNLETNKIESFIKF 175 Score = 139 bits (337), Expect = 3e-34 Identities = 61/84 (72%), Positives = 70/84 (83%) Frame = +2 Query: 2 KHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVK 181 KHLKR+ AP W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G EV I+ Sbjct: 7 KHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGREVKAILM 66 Query: 182 QRLIKVDGKVRTDPTYPAGFMDVV 253 QRLI+VDGKVRTD T+P GFMDV+ Sbjct: 67 QRLIQVDGKVRTDSTFPTGFMDVI 90 Score = 80.2 bits (189), Expect = 3e-16 Identities = 34/52 (65%), Positives = 43/52 (82%) Frame = +3 Query: 525 MITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKGTK 680 M+TGGRN+GRVGTIV RE H GSF+I+H+KD+ FATRL+NVF+IG+ K Sbjct: 182 MVTGGRNMGRVGTIVHREHHLGSFEIIHVKDALDREFATRLSNVFVIGEAGK 233 >SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein S4|Schizosaccharomyces pombe|chr 1|||Manual Length = 262 Score = 142 bits (344), Expect = 4e-35 Identities = 63/84 (75%), Positives = 73/84 (86%) Frame = +1 Query: 256 IEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPD 435 ++KT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKRV G K VP+LVTHDGRTIRYPD Sbjct: 92 VDKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKRVQLGAKGVPFLVTHDGRTIRYPD 151 Query: 436 PLIKVNDSIQLDIATTKIMDFIKF 507 PLIKVND+I+L++ T KI FIKF Sbjct: 152 PLIKVNDTIKLNLETNKIESFIKF 175 Score = 141 bits (341), Expect = 1e-34 Identities = 62/84 (73%), Positives = 70/84 (83%) Frame = +2 Query: 2 KHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVK 181 KHLKR+ AP W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G EV I+ Sbjct: 7 KHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGREVKAILM 66 Query: 182 QRLIKVDGKVRTDPTYPAGFMDVV 253 QRLIKVDGKVRTD T+P GFMDV+ Sbjct: 67 QRLIKVDGKVRTDSTFPTGFMDVI 90 Score = 79.4 bits (187), Expect = 5e-16 Identities = 34/52 (65%), Positives = 43/52 (82%) Frame = +3 Query: 525 MITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKGTK 680 M+TGGRN+GRVGTIV RE H GSF+I+H+KD+ FATRL+NVF+IG+ K Sbjct: 182 MVTGGRNMGRVGTIVHREHHLGSFEIIHVKDALDREFATRLSNVFVIGETGK 233 >SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces pombe|chr 2|||Manual Length = 1014 Score = 28.7 bits (61), Expect = 0.86 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -2 Query: 662 YEHVVQPRGEGVSRGVLDVHNVEGAGMSLAGHD 564 Y V+ P EG LD+H+ A ++L GH+ Sbjct: 901 YRVVLHPEHEGAVVEFLDIHDAGKASLALEGHE 933 >SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1338 Score = 27.5 bits (58), Expect = 2.0 Identities = 17/55 (30%), Positives = 30/55 (54%), Gaps = 3/55 (5%) Frame = -3 Query: 238 SSRISRVSPNFPIN--LYEALFH-NFQDFVSGQSILQTIPQENHQGQALAQLVGT 83 SS+ + P+ P++ +E F NFQ S L IP++ G ++A+++GT Sbjct: 27 SSKDENLQPSIPLSPVAFELDFSGNFQFISDNSSELLDIPKDKIIGHSVAEVLGT 81 >SPAC806.06c |||nicotinamide mononucleotide |Schizosaccharomyces pombe|chr 1|||Manual Length = 365 Score = 27.5 bits (58), Expect = 2.0 Identities = 18/58 (31%), Positives = 30/58 (51%) Frame = +1 Query: 340 KYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFGL 513 K+ C V+R TG +L+ HD Y ++ + I DI++TK+ FI+ G+ Sbjct: 271 KFGCCIVER--TGSDVWAFLLAHD-IMFAYRGNILVIKQLIYNDISSTKVRLFIRRGM 325 >SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1428 Score = 26.6 bits (56), Expect = 3.5 Identities = 8/15 (53%), Positives = 12/15 (80%) Frame = +3 Query: 444 QSQRFHPVRHRNYED 488 ++++ HPVRH YED Sbjct: 5 ENEKIHPVRHSKYED 19 >SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 26.2 bits (55), Expect = 4.6 Identities = 9/15 (60%), Positives = 10/15 (66%) Frame = +3 Query: 411 WPHHPLPRPTYQSQR 455 WPHH LP + SQR Sbjct: 265 WPHHNLPSQFFTSQR 279 >SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizosaccharomyces pombe|chr 3|||Manual Length = 640 Score = 25.8 bits (54), Expect = 6.0 Identities = 20/86 (23%), Positives = 33/86 (38%) Frame = -2 Query: 317 WIVNLPLTS*IRRNSSLVFSIRQHP*IQQDK*GQS*LSHQPL*GVVSQFSGLRFRSKHTS 138 W+V + SL+ P ++ K G + + G + + L ++ HTS Sbjct: 237 WLVEFSSVCQAAESISLIAVNNNRPPVELPKSGDVLSNREWEAGKIDAMNSLIAQNLHTS 296 Query: 137 DDSSGKSPGASTRATCGDRLTVSVHT 60 SP AST ++ V HT Sbjct: 297 ASQVSLSPMASTASSSVTNSPVDTHT 322 >SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces pombe|chr 3|||Manual Length = 964 Score = 25.4 bits (53), Expect = 8.0 Identities = 9/26 (34%), Positives = 14/26 (53%) Frame = +3 Query: 414 PHHPLPRPTYQSQRFHPVRHRNYEDY 491 PH+ P + Q+ HPV+ N+ Y Sbjct: 265 PHNSFPASADRLQKNHPVQSSNFNPY 290 >SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1953 Score = 25.4 bits (53), Expect = 8.0 Identities = 16/45 (35%), Positives = 19/45 (42%) Frame = +1 Query: 571 PARDIPAPSTLCTSRTPRDTPSPRG*TTCS*SARARSVHLAAARQ 705 P IP T + P DTP+P SAR R+ ARQ Sbjct: 250 PTTSIPEHKTSINNNKPEDTPTPS--ENVHLSARQRNALKRKARQ 292 >SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1261 Score = 25.4 bits (53), Expect = 8.0 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = -1 Query: 339 SLLRGDTVDCESALNIID*TKQ 274 S RGDT D +ALNI + TK+ Sbjct: 898 STRRGDTDDATTALNIFEETKR 919 >SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 910 Score = 25.4 bits (53), Expect = 8.0 Identities = 10/40 (25%), Positives = 21/40 (52%) Frame = -3 Query: 259 QLDNIHKSSRISRVSPNFPINLYEALFHNFQDFVSGQSIL 140 Q +N S++ P I+LY L+H+ +++ Q ++ Sbjct: 399 QYENSLSLLETSKIDPRVVISLYPDLYHSELSYIAFQGVI 438 >SPBC18H10.10c |cwc16||complexed with Cdc5 protein Cwf16|Schizosaccharomyces pombe|chr 2|||Manual Length = 294 Score = 25.4 bits (53), Expect = 8.0 Identities = 18/62 (29%), Positives = 23/62 (37%), Gaps = 1/62 (1%) Frame = +1 Query: 235 WIYGCCLIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVK-RVATGPKNVPYLVTHD 411 W C I + F + G + +I K LC V T PKN Y+V Sbjct: 32 WCNNCENIIQQGTRFNAVKKEIGSYYTTKIWSFSLKCHLCSNPIDVHTDPKNTEYIVASG 91 Query: 412 GR 417 GR Sbjct: 92 GR 93 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,235,432 Number of Sequences: 5004 Number of extensions: 71374 Number of successful extensions: 209 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 194 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 209 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 327172622 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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