Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00124
(769 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 128 7e-31
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 122 6e-29
SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces pomb... 29 0.55
SPAC5H10.09c |||3-methyl-2-oxobutanoatehydroxymethyltransferase|... 27 3.0
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 27 3.9
SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p... 27 3.9
SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces p... 26 5.2
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 26 5.2
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 26 6.8
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 25 9.0
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 25 9.0
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 128 bits (310), Expect = 7e-31
Identities = 63/89 (70%), Positives = 77/89 (86%)
Frame = +2
Query: 497 GHRRTRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLA 676
G +R +HS + S + Y+ A EI+ A++ PTHPIRLGLALNFSVFYYEILNSPD+AC LA
Sbjct: 140 GEKR-QHSA-DQSLEGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLA 197
Query: 677 KQAFDDAIAELDTLNEDSYKDSTLIMQLL 763
KQAFD+AI+ELD+L+E+SYKDSTLIMQLL
Sbjct: 198 KQAFDEAISELDSLSEESYKDSTLIMQLL 226
Score = 120 bits (288), Expect = 3e-28
Identities = 55/87 (63%), Positives = 70/87 (80%), Gaps = 2/87 (2%)
Frame = +3
Query: 255 VGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKA 428
+GARR+SWR++SSIEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KHLIP A
Sbjct: 55 IGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNA 114
Query: 429 SNPESKVFYLKMKGDYYRYLAEVATGE 509
++ ESKVFY KMKGDYYRYLAE A GE
Sbjct: 115 ASAESKVFYYKMKGDYYRYLAEFAVGE 141
Score = 63.3 bits (147), Expect = 4e-11
Identities = 32/52 (61%), Positives = 37/52 (71%)
Frame = +1
Query: 100 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNV 255
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAYKNV
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNV 54
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 122 bits (294), Expect = 6e-29
Identities = 57/87 (65%), Positives = 69/87 (79%), Gaps = 2/87 (2%)
Frame = +3
Query: 255 VGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKA 428
+GARR+SWR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KHLIP A
Sbjct: 54 IGARRASWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAA 113
Query: 429 SNPESKVFYLKMKGDYYRYLAEVATGE 509
+ ESKVFY KMKGDYYRYLAE GE
Sbjct: 114 TTGESKVFYYKMKGDYYRYLAEFTVGE 140
Score = 116 bits (280), Expect = 3e-27
Identities = 54/79 (68%), Positives = 69/79 (87%)
Frame = +2
Query: 527 EDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAE 706
+ S +AY+ A +I+ A++ PT P+RLGLALNFSVFYYEIL+SP+ AC LAKQ FD+AI+E
Sbjct: 147 DSSLEAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISE 206
Query: 707 LDTLNEDSYKDSTLIMQLL 763
LD+L+E+SYKDSTLIMQLL
Sbjct: 207 LDSLSEESYKDSTLIMQLL 225
Score = 61.7 bits (143), Expect = 1e-10
Identities = 32/53 (60%), Positives = 39/53 (73%)
Frame = +1
Query: 97 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNV 255
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSVAYKN+
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNI 53
>SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 605
Score = 29.5 bits (63), Expect = 0.55
Identities = 16/38 (42%), Positives = 22/38 (57%)
Frame = -2
Query: 558 KASWYAFCESSTTECLVLLWPLLQGTCSNLLSF*GKTL 445
+A W AF E+S +E + L +L S+LLSF TL
Sbjct: 64 EAVWAAFSEASCSERKLALQGILNNCSSSLLSFASSTL 101
>SPAC5H10.09c
|||3-methyl-2-
oxobutanoatehydroxymethyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 267
Score = 27.1 bits (57), Expect = 3.0
Identities = 13/35 (37%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 466 KEITTGTLQKWPQENQTFCC-RGFTESIPRCF*NQ 567
K+IT TL++W N+ F C + S R F Q
Sbjct: 4 KQITISTLRQWKLANKKFACITAYDASFSRLFAEQ 38
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 26.6 bits (56), Expect = 3.9
Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = +2
Query: 467 RRLLQVPCRSGHRRTRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFS 625
R ++ + C RR ED+++A + ++ + A +QP H I L LA+N S
Sbjct: 1049 RYIVALSCELERRRLGE---EDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099
>SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 26.6 bits (56), Expect = 3.9
Identities = 21/69 (30%), Positives = 37/69 (53%), Gaps = 2/69 (2%)
Frame = +3
Query: 297 EQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM-KGD 473
E E+ Q++A+ Y+V + +LRE YD LG + +P A ++ F+ + GD
Sbjct: 43 ENPEAAREKFQKLAEAYQVLSDPKLRE-KYDKLGKVG--AVPDAGFEDAFEFFKNLFGGD 99
Query: 474 YYR-YLAEV 497
+R Y+ E+
Sbjct: 100 SFRDYVGEL 108
>SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 517
Score = 26.2 bits (55), Expect = 5.2
Identities = 8/40 (20%), Positives = 20/40 (50%)
Frame = -3
Query: 722 RSMCPVRRWHRRKPVWRVDTPCLANLISHNKRLRNLTPDP 603
R + P+ +W R++ +W + P + + ++ + P P
Sbjct: 120 RDVTPINQWKRKRSLWDIKPPGYELVTADQAKMSGVFPLP 159
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.2 bits (55), Expect = 5.2
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +3
Query: 300 QKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 425
+K + + KEY K +++LR+ D+ LL KH I +
Sbjct: 328 EKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 25.8 bits (54), Expect = 6.8
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = +2
Query: 2 EKLILCLNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNW 139
EKL+ C HF +G+ + L RC +T CNVP W
Sbjct: 683 EKLVACTT-----HFY---EGVRKSALSSLWRCATTYYKVCNVPQW 720
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 9.0
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +2
Query: 44 FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 166
FSP +K + +L LFH + PS+++ V N + SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201
>SPBC6B1.04 |mde4||monopolin-like complex subunit
Mde4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 421
Score = 25.4 bits (53), Expect = 9.0
Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +3
Query: 318 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 440
E++Q A +YR+KVE+ +I V + L+ L + SNPE
Sbjct: 85 EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,166,707
Number of Sequences: 5004
Number of extensions: 64763
Number of successful extensions: 230
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 228
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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