BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00118 (391 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.14c |btf3|egd1, btt1, nac2|nascent polypeptide-associat... 50 1e-07 SPAC977.17 |||MIP water channel|Schizosaccharomyces pombe|chr 1|... 27 0.77 SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces p... 25 3.1 SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces... 24 7.2 SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosacc... 24 7.2 SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 24 9.5 SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex al... 24 9.5 >SPAC4F10.14c |btf3|egd1, btt1, nac2|nascent polypeptide-associated complex beta subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 50.4 bits (115), Expect = 1e-07 Identities = 27/46 (58%), Positives = 32/46 (69%), Gaps = 2/46 (4%) Frame = +1 Query: 124 MNSEKLKKLQSQVRIGGKGTPRRKKKVVHVTA--ATDDKKLQSSLK 255 M+ KL KLQ+ RIGGKGTPRRK K +A A DDKK+Q +LK Sbjct: 1 MDPSKLAKLQAGARIGGKGTPRRKVKKPSKSAMSAADDKKVQGALK 46 Score = 46.4 bits (105), Expect = 2e-06 Identities = 22/44 (50%), Positives = 29/44 (65%) Frame = +3 Query: 252 QKLSVNTIPGIEEVNMIKEDGTVIHFNNPKAQASLAANTFAITG 383 +KL++ + GI+EVNM KEDG VI+F P +SL T AI G Sbjct: 46 KKLNMQNLAGIQEVNMFKEDGGVINFRAPTVHSSLPNETTAIYG 89 >SPAC977.17 |||MIP water channel|Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 27.5 bits (58), Expect = 0.77 Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +1 Query: 61 NSVIKKCPVLTLTQFTLKNSRMNSEKLKKLQSQVRIGG-KGTPRRKKKV 204 NSV++K + NSR+NS + S V + G G+P K+K+ Sbjct: 131 NSVVEKISQKNQEARSRANSRVNSRANSRANSSVSLAGMDGSPNWKRKM 179 >SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 202 Score = 25.4 bits (53), Expect = 3.1 Identities = 10/31 (32%), Positives = 18/31 (58%) Frame = +1 Query: 112 KNSRMNSEKLKKLQSQVRIGGKGTPRRKKKV 204 K + +KLKK ++++ T +RK+KV Sbjct: 148 KEKKEKKDKLKKKSKRLKLDDSHTQKRKRKV 178 >SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces pombe|chr 3|||Manual Length = 815 Score = 24.2 bits (50), Expect = 7.2 Identities = 12/49 (24%), Positives = 22/49 (44%) Frame = +1 Query: 103 FTLKNSRMNSEKLKKLQSQVRIGGKGTPRRKKKVVHVTAATDDKKLQSS 249 F +K S+ + + + SQ+ IGG P +K+ D +Q + Sbjct: 77 FAMKRSQTSVQP--SMSSQIAIGGMRNPMATRKIAGTYRDAPDNSMQQT 123 >SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 24.2 bits (50), Expect = 7.2 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = -2 Query: 204 NLLLASRCALTADAYLRLEFL*FFAVHSAVFKRK 103 N +LASR LT +LRL L + VF ++ Sbjct: 107 NTMLASRAPLTVPMFLRLSDLKLNGIVVLVFSKQ 140 >SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 339 Score = 23.8 bits (49), Expect = 9.5 Identities = 9/17 (52%), Positives = 11/17 (64%) Frame = -1 Query: 220 PQ*REQPSSCVSVCPYR 170 P EQP+ CV+ CP R Sbjct: 164 PSRNEQPAFCVNWCPSR 180 >SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex alpha subunit Egd2|Schizosaccharomyces pombe|chr 2|||Manual Length = 173 Score = 23.8 bits (49), Expect = 9.5 Identities = 13/51 (25%), Positives = 23/51 (45%) Frame = +3 Query: 231 QKTAVIAQKLSVNTIPGIEEVNMIKEDGTVIHFNNPKAQASLAANTFAITG 383 +K + QKL + + GI V M + ++ N P S + N + + G Sbjct: 25 EKAQKLIQKLGLKRVEGITRVAMRRPKNILLIINEPIVYKS-SNNAYIVLG 74 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,523,460 Number of Sequences: 5004 Number of extensions: 27673 Number of successful extensions: 69 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 67 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 69 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 128029482 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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