Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00112
(690 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 151 3e-37
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 151 3e-37
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 151 3e-37
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 151 3e-37
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 98 6e-21
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 92 4e-19
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 69 2e-12
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 66 2e-11
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 36 0.025
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 36 0.025
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 36 0.025
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 36 0.025
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 36 0.025
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 35 0.058
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 35 0.058
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 35 0.058
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 33 0.18
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.18
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.24
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.24
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 31 0.95
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.7
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.7
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.7
At5g64600.1 68418.m08118 expressed protein similar to axi 1 [Nic... 29 2.2
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.2
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 29 2.2
At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containi... 28 5.1
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 5.1
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 6.7
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 6.7
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 27 8.9
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 27 8.9
At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha s... 27 8.9
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 151 bits (367), Expect = 3e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 436
ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 437 GQTREHALLAFTLGVKQLIVGVNK 508
GQTREHALLAFTLGVKQ+I NK
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNK 154
Score = 132 bits (318), Expect = 3e-31
Identities = 62/69 (89%), Positives = 63/69 (91%)
Frame = +3
Query: 48 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 227
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 228 DKLKAERER 254
DKLKAERER
Sbjct: 61 DKLKAERER 69
Score = 90.2 bits (214), Expect = 1e-18
Identities = 37/60 (61%), Positives = 49/60 (81%)
Frame = +1
Query: 505 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 684
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 151 bits (367), Expect = 3e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 436
ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 437 GQTREHALLAFTLGVKQLIVGVNK 508
GQTREHALLAFTLGVKQ+I NK
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNK 154
Score = 132 bits (318), Expect = 3e-31
Identities = 62/69 (89%), Positives = 63/69 (91%)
Frame = +3
Query: 48 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 227
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 228 DKLKAERER 254
DKLKAERER
Sbjct: 61 DKLKAERER 69
Score = 90.2 bits (214), Expect = 1e-18
Identities = 37/60 (61%), Positives = 49/60 (81%)
Frame = +1
Query: 505 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 684
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 151 bits (367), Expect = 3e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 436
ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 437 GQTREHALLAFTLGVKQLIVGVNK 508
GQTREHALLAFTLGVKQ+I NK
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNK 154
Score = 132 bits (318), Expect = 3e-31
Identities = 62/69 (89%), Positives = 63/69 (91%)
Frame = +3
Query: 48 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 227
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 228 DKLKAERER 254
DKLKAERER
Sbjct: 61 DKLKAERER 69
Score = 90.2 bits (214), Expect = 1e-18
Identities = 37/60 (61%), Positives = 49/60 (81%)
Frame = +1
Query: 505 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 684
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 151 bits (367), Expect = 3e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 436
ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+
Sbjct: 71 ITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKD 130
Query: 437 GQTREHALLAFTLGVKQLIVGVNK 508
GQTREHALLAFTLGVKQ+I NK
Sbjct: 131 GQTREHALLAFTLGVKQMICCCNK 154
Score = 132 bits (318), Expect = 3e-31
Identities = 62/69 (89%), Positives = 63/69 (91%)
Frame = +3
Query: 48 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 227
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 228 DKLKAERER 254
DKLKAERER
Sbjct: 61 DKLKAERER 69
Score = 90.2 bits (214), Expect = 1e-18
Identities = 37/60 (61%), Positives = 49/60 (81%)
Frame = +1
Query: 505 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 684
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 97.9 bits (233), Expect = 6e-21
Identities = 45/83 (54%), Positives = 58/83 (69%)
Frame = +2
Query: 260 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 439
T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEFE G + G
Sbjct: 166 TVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYERGG 225
Query: 440 QTREHALLAFTLGVKQLIVGVNK 508
QTREH LA TLGV +LIV VNK
Sbjct: 226 QTREHVQLAKTLGVSKLIVVVNK 248
Score = 68.1 bits (159), Expect = 5e-12
Identities = 28/64 (43%), Positives = 46/64 (71%)
Frame = +3
Query: 57 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 236
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 237 KAER 248
+ ER
Sbjct: 158 EEER 161
Score = 41.5 bits (93), Expect = 5e-04
Identities = 22/63 (34%), Positives = 37/63 (58%), Gaps = 3/63 (4%)
Frame = +1
Query: 505 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAA-VAFVPISGWHGDNMLEPSTK--MPW 675
+MD +S+ R++EI++++ ++K GYN V F+PISG G NM + + PW
Sbjct: 248 KMDDPTVNWSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISGLMGKNMDQRMGQEICPW 307
Query: 676 FKG 684
+ G
Sbjct: 308 WSG 310
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 91.9 bits (218), Expect = 4e-19
Identities = 40/85 (47%), Positives = 60/85 (70%), Gaps = 1/85 (1%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK- 433
IT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEAG
Sbjct: 303 ITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGFDNL 362
Query: 434 NGQTREHALLAFTLGVKQLIVGVNK 508
GQTREHA + GV+Q+IV +NK
Sbjct: 363 KGQTREHARVLRGFGVEQVIVAINK 387
Score = 79.4 bits (187), Expect = 2e-15
Identities = 33/64 (51%), Positives = 47/64 (73%)
Frame = +3
Query: 63 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 242
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 243 ERER 254
ERER
Sbjct: 298 ERER 301
Score = 34.7 bits (76), Expect = 0.058
Identities = 15/55 (27%), Positives = 32/55 (58%), Gaps = 3/55 (5%)
Frame = +1
Query: 529 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML-EPSTK--MPWFKG 684
YS+ RF+ IK+ V S+++ + +++ ++P+S N++ PS W++G
Sbjct: 393 YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLSAMENQNLVAAPSDNRLSSWYQG 447
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 69.3 bits (162), Expect = 2e-12
Identities = 35/84 (41%), Positives = 51/84 (60%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 436
ITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ G
Sbjct: 128 ITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMP------ 181
Query: 437 GQTREHALLAFTLGVKQLIVGVNK 508
QT+EH LLA +GV ++V +NK
Sbjct: 182 -QTKEHILLAKQVGVPDMVVFLNK 204
Score = 39.1 bits (87), Expect = 0.003
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +3
Query: 45 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 179
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 66.1 bits (154), Expect = 2e-11
Identities = 35/84 (41%), Positives = 50/84 (59%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 436
ITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 116 ITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMP------ 169
Query: 437 GQTREHALLAFTLGVKQLIVGVNK 508
QT+EH LLA +GV L+ +NK
Sbjct: 170 -QTKEHILLARQVGVPSLVCFLNK 192
Score = 32.7 bits (71), Expect = 0.24
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +3
Query: 54 KEKTHINIVVIGHVDSGKSTTT 119
+ K H+N+ IGHVD GK+T T
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT 84
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 35.9 bits (79), Expect = 0.025
Identities = 24/93 (25%), Positives = 45/93 (48%)
Frame = +2
Query: 290 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 469
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 470 TLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKY 568
+ +K +I+ NK I L + + + ++ +K+
Sbjct: 178 MMRLKHIIILQNK-IDLINEKAATEQHEAIQKF 209
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 35.9 bits (79), Expect = 0.025
Identities = 24/93 (25%), Positives = 45/93 (48%)
Frame = +2
Query: 290 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 469
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 470 TLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKY 568
+ +K +I+ NK I L + + + ++ +K+
Sbjct: 178 MMRLKHIIILQNK-IDLINEKAATEQHEAIQKF 209
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 35.9 bits (79), Expect = 0.025
Identities = 26/89 (29%), Positives = 37/89 (41%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 436
ITI A Y V IID PGH DF + D A+L++ + G I+ +
Sbjct: 119 ITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVD 178
Query: 437 GQTREHALLAFTLGVKQLIVGVNKWIPLN 523
Q R + + K +G + W LN
Sbjct: 179 RQMRRYEVPRVAFINKLDRMGADPWKVLN 207
Score = 27.9 bits (59), Expect = 6.7
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 72 NIVVIGHVDSGKSTTTGHLIYKCGGI 149
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 35.9 bits (79), Expect = 0.025
Identities = 26/89 (29%), Positives = 37/89 (41%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 436
ITI A Y V IID PGH DF + D A+L++ + G I+ +
Sbjct: 119 ITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVD 178
Query: 437 GQTREHALLAFTLGVKQLIVGVNKWIPLN 523
Q R + + K +G + W LN
Sbjct: 179 RQMRRYEVPRVAFINKLDRMGADPWKVLN 207
Score = 27.9 bits (59), Expect = 6.7
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 72 NIVVIGHVDSGKSTTTGHLIYKCGGI 149
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 35.9 bits (79), Expect = 0.025
Identities = 28/99 (28%), Positives = 47/99 (47%), Gaps = 4/99 (4%)
Frame = +2
Query: 284 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 451
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 452 HALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKY 568
H + +K +I+ NK I L V+ + ++ +K+
Sbjct: 166 HLAAVEIMQLKHIIILQNK-IDLIQENVAINQHEAIQKF 203
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 34.7 bits (76), Expect = 0.058
Identities = 21/69 (30%), Positives = 33/69 (47%)
Frame = +2
Query: 302 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 481
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 482 KQLIVGVNK 508
K +I+ NK
Sbjct: 174 KDIIIIQNK 182
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 34.7 bits (76), Expect = 0.058
Identities = 18/51 (35%), Positives = 24/51 (47%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 409
+ + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 195 VPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 34.7 bits (76), Expect = 0.058
Identities = 18/51 (35%), Positives = 24/51 (47%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 409
+ + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 195 VPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 33.1 bits (72), Expect = 0.18
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = +2
Query: 287 ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 409
E S Y + +ID PGH DF + S A+L+V A G
Sbjct: 131 EASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQG 171
Score = 27.9 bits (59), Expect = 6.7
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +3
Query: 36 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 155
D K EK N +I H+D GKST L+ G I K
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 33.1 bits (72), Expect = 0.18
Identities = 16/51 (31%), Positives = 27/51 (52%)
Frame = +2
Query: 257 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 409
IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 60 ITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
Score = 32.7 bits (71), Expect = 0.24
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +3
Query: 72 NIVVIGHVDSGKSTTTGHLIYKCGG 146
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.24
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 305 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 409
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 32.7 bits (71), Expect = 0.24
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +3
Query: 60 KTHINIVVIGHVDSGKSTTTGHLIYKCG 143
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 30.7 bits (66), Expect = 0.95
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +2
Query: 284 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 409
+E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 147 YEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
Score = 28.3 bits (60), Expect = 5.1
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +3
Query: 72 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEK 170
N +I H+D GKST L+ G + R +++
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKE 120
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 311 IIDAPGHRDFIKNMITGTSQADCAVLIV 394
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 311 IIDAPGHRDFIKNMITGTSQADCAVLIV 394
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 311 IIDAPGHRDFIKNMITGTSQADCAVLIV 394
+ID PGH F G+S D A+L+V
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV 732
>At5g64600.1 68418.m08118 expressed protein similar to axi 1
[Nicotiana tabacum] GI:559921; contains Pfam profile
PF03138: Plant protein family
Length = 522
Score = 29.5 bits (63), Expect = 2.2
Identities = 19/68 (27%), Positives = 34/68 (50%), Gaps = 2/68 (2%)
Frame = +1
Query: 475 RCQTAHRRSKQMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG--DNM 648
R T+H + K ++STE E KEV ++K +GY+ + V ++ +G D +
Sbjct: 313 RESTSHWKIKSINSTEQR-EEGLCPLTPKEVGIFLKGLGYSQSTVIYIAAGEIYGGDDRL 371
Query: 649 LEPSTKMP 672
E ++ P
Sbjct: 372 SELKSRFP 379
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +2
Query: 293 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 394
++Y + +ID+PGH DF + D A+++V
Sbjct: 96 NEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
Score = 29.1 bits (62), Expect = 2.9
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +3
Query: 72 NIVVIGHVDSGKSTTTGHLIYKCG 143
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +2
Query: 305 VTIIDAPGHRDFIKNMITGTSQADCAVLIV 394
+ +ID PGH F G++ D A+L+V
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV 587
>At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containing
protein contains Pfam profile PF01388: ARID/BRIGHT DNA
binding domain
Length = 747
Score = 28.3 bits (60), Expect = 5.1
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +3
Query: 585 DWLQPSCCRFRAHFWMARRQHVGAFNQNALVQGM 686
DW+ C AHF RR +GAF A G+
Sbjct: 687 DWVNCGSCGEWAHFGCDRRPGLGAFKDYAKTDGL 720
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 5.1
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +1
Query: 532 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 621
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 27.9 bits (59), Expect = 6.7
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 538 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 648
PRF ++ ++SS ++ Y AAVAF +S W D +
Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 27.9 bits (59), Expect = 6.7
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +2
Query: 314 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 409
+D PGH F G D A+++VAA G
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG 588
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 27.5 bits (58), Expect = 8.9
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +1
Query: 514 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 627
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 27.5 bits (58), Expect = 8.9
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +1
Query: 514 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 627
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha
subunit, chloroplast / 60 kDa chaperonin alpha subunit /
CPN-60 alpha identical to SWISS-PROT:P21238- RuBisCO
subunit binding-protein alpha subunit, chloroplast
precursor (60 kDa chaperonin alpha subunit, CPN-60
alpha) [Arabidopsis thaliana]
Length = 586
Score = 27.5 bits (58), Expect = 8.9
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = -3
Query: 397 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDCDTRSRSAFSLSN 224
++D+HS +L+ D + D + ++ N+VL F P+ D T +R A L N
Sbjct: 52 AFDQHSRAALQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIAR-AIELPN 108
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,548,089
Number of Sequences: 28952
Number of extensions: 329826
Number of successful extensions: 1132
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 1067
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1128
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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