Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00097
(698 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 44 1e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 41 7e-04
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 38 0.006
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 37 0.011
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 37 0.015
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 34 0.079
At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17... 33 0.18
At4g04990.1 68417.m00728 expressed protein contains Pfam domain ... 32 0.42
At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 29 3.0
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 3.9
At4g31070.1 68417.m04411 pentatricopeptide (PPR) repeat-containi... 28 5.2
At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 28 5.2
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 28 5.2
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 9.0
At1g34180.1 68414.m04239 no apical meristem (NAM) family protein... 27 9.0
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 43.6 bits (98), Expect = 1e-04
Identities = 30/81 (37%), Positives = 46/81 (56%), Gaps = 8/81 (9%)
Frame = +3
Query: 273 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 428
D+ EE+ V+ DG I+ + G+ +EEK D+ + R +FTRR+ LPE E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 429 SVESRLSSDGVLSVIAPRKVP 491
+++ + +GVLSV P KVP
Sbjct: 126 EIKASM-ENGVLSVTVP-KVP 144
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 41.1 bits (92), Expect = 7e-04
Identities = 28/78 (35%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +3
Query: 273 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 428
DV EE+ V+ DG I+ + G+ +EEK D + R +F RR+ LPE E
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 429 SVESRLSSDGVLSVIAPR 482
V++ + +GVLSV P+
Sbjct: 125 EVKASM-ENGVLSVTVPK 141
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 37.9 bits (84), Expect = 0.006
Identities = 27/90 (30%), Positives = 50/90 (55%), Gaps = 8/90 (8%)
Frame = +3
Query: 261 QVNLDVQHFAPEEISVKTAD-GYIVVEG---KHEEKK-DQHGYISR---QFTRRYALPEG 416
++ LD+ +E+ ++ + G + V G + EEKK DQ + R +F R++ LP+
Sbjct: 83 EIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWRQFKLPDN 142
Query: 417 CTAESVESRLSSDGVLSVIAPRKVPPAVEG 506
ESV+++L +GVL++ + P V+G
Sbjct: 143 VDMESVKAKL-ENGVLTINLTKLSPEKVKG 171
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 37.1 bits (82), Expect = 0.011
Identities = 24/78 (30%), Positives = 43/78 (55%), Gaps = 8/78 (10%)
Frame = +3
Query: 273 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 428
D+ EE+ V+ D ++ + G+ +EEK D+ + R +F RR+ LPE E
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 429 SVESRLSSDGVLSVIAPR 482
V++ + +GVL+V+ P+
Sbjct: 128 EVKATM-ENGVLTVVVPK 144
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 36.7 bits (81), Expect = 0.015
Identities = 25/78 (32%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +3
Query: 273 DVQHFAPEEISVKTA-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 428
D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE +
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 429 SVESRLSSDGVLSVIAPR 482
V++ + +GVL+V P+
Sbjct: 122 QVKAAM-ENGVLTVTVPK 138
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 34.3 bits (75), Expect = 0.079
Identities = 23/78 (29%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +3
Query: 273 DVQHFAPEEISVKTADGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 428
D+ EE+ V+ D ++ + G+ EEK+D + R QF+R++ LPE +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 429 SVESRLSSDGVLSVIAPR 482
V++ + +GVL+V P+
Sbjct: 124 QVKASM-ENGVLTVTVPK 140
>At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17.6A
(HSP17.7-CII) identical to heat shock protein 17.6A
GI:3256075 from [Arabidopsis thaliana]
Length = 156
Score = 33.1 bits (72), Expect = 0.18
Identities = 25/84 (29%), Positives = 42/84 (50%), Gaps = 9/84 (10%)
Frame = +3
Query: 270 LDVQHFAPEEISVKTA-DGYIVVEGKHE-EKKDQHGY----ISRQ---FTRRYALPEGCT 422
+D+ +EI V+ + +VV GK + + K+ G + R+ F R++ LP+
Sbjct: 61 VDMPGIKGDEIQVQIENENVLVVSGKRQRDNKENEGVKFVRMERRMGKFMRKFQLPDNAD 120
Query: 423 AESVESRLSSDGVLSVIAPRKVPP 494
E + S +DGVL V P+ PP
Sbjct: 121 LEKI-SAACNDGVLKVTIPKLPPP 143
>At4g04990.1 68417.m00728 expressed protein contains Pfam domain
PF05553: Cotton fiber expressed protein
Length = 303
Score = 31.9 bits (69), Expect = 0.42
Identities = 22/64 (34%), Positives = 31/64 (48%)
Frame = +2
Query: 89 ERPRRLMDQHFGLGLTPEDFLSAAAGPLVSREYYRPWVTLLPRLETLAPASKVTRKVPSK 268
++P L D G T +S ++ PL SR R P L +L+P+S R+ PS
Sbjct: 183 KKPNSLQDHVISRGDTK---MSTSSWPLPSRSPSRARRPT-PSLSSLSPSSSRARRPPSS 238
Query: 269 PGRP 280
P RP
Sbjct: 239 PARP 242
>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 478
Score = 29.1 bits (62), Expect = 3.0
Identities = 23/59 (38%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = -3
Query: 438 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPG 268
T I+ YS A I N A L P+ PR S PPF LPG+NAG G
Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADG 72
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.7 bits (61), Expect = 3.9
Identities = 17/54 (31%), Positives = 23/54 (42%)
Frame = +3
Query: 294 EEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 455
+EI D Y EG EE+ ++ R + L E C E+VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At4g31070.1 68417.m04411 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 613
Score = 28.3 bits (60), Expect = 5.2
Identities = 16/51 (31%), Positives = 25/51 (49%)
Frame = +2
Query: 173 VSREYYRPWVTLLPRLETLAPASKVTRKVPSKPGRPAFRPGRNLC*NGGRL 325
V+ E+Y ++ LL R + A +VT +P KP + + C GRL
Sbjct: 487 VTLEHYACYINLLGRFGKIDDAFEVTINMPMKPSARIWSSLLSACETHGRL 537
>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 28.3 bits (60), Expect = 5.2
Identities = 21/71 (29%), Positives = 33/71 (46%), Gaps = 3/71 (4%)
Frame = +3
Query: 138 RKIFSVLQPAPL*AENTTARGSPCCRGSRPWLQHQK*QGKFQ---VNLDVQHFAPEEISV 308
R F L+P L + + R + C SRPWL + +G F ++ + FA S
Sbjct: 685 RSRFLGLKPPKLFSISVRGRSAMLCLSSRPWLGYIH-RGHFHLTPLSYETLEFAAPFSSD 743
Query: 309 KTADGYIVVEG 341
+ A+G + V G
Sbjct: 744 QCAEGVVSVAG 754
>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 28.3 bits (60), Expect = 5.2
Identities = 21/71 (29%), Positives = 33/71 (46%), Gaps = 3/71 (4%)
Frame = +3
Query: 138 RKIFSVLQPAPL*AENTTARGSPCCRGSRPWLQHQK*QGKFQ---VNLDVQHFAPEEISV 308
R F L+P L + + R + C SRPWL + +G F ++ + FA S
Sbjct: 685 RSRFLGLKPPKLFSISVRGRSAMLCLSSRPWLGYIH-RGHFHLTPLSYETLEFAAPFSSD 743
Query: 309 KTADGYIVVEG 341
+ A+G + V G
Sbjct: 744 QCAEGVVSVAG 754
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = -3
Query: 459 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 316
HHL + ++ P S P S + H SS PRCS+P
Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73
>At1g34180.1 68414.m04239 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
similar to NAM-like protein GI:8809651 from (Arabidopsis
thaliana)
Length = 564
Score = 27.5 bits (58), Expect = 9.0
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = +3
Query: 333 VEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDGVLSVIAPR 482
+E E D G RQF +P+G +AE ++S L ++ I PR
Sbjct: 229 IEKLLNEIPDAPGVNQRQFDEFVGVPQGNSAEVIQSTLLNNSSGEYIDPR 278
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,511,118
Number of Sequences: 28952
Number of extensions: 344605
Number of successful extensions: 958
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 917
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 958
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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