SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00095
         (634 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p...   138   5e-34
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ...   131   9e-32
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc...   118   9e-28
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M...   113   2e-26
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce...    83   4e-17
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch...    52   7e-08
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom...    44   2e-05
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe...    30   0.32 
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    29   0.42 
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar...    29   0.74 
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo...    27   1.7  
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar...    25   6.9  
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar...    25   6.9  
SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|...    25   9.1  
SPBC776.10c |cog6||Golgi transport complex peripheral subunit Co...    25   9.1  

>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 647

 Score =  138 bits (335), Expect = 5e-34
 Identities = 63/88 (71%), Positives = 73/88 (82%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
           P   IFDAKRLIGRKF+D  VQ+DMKHWPF+V+S  GKP ++V YKGE KTF PEE+SSM
Sbjct: 61  PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120

Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
           VL KM+ETAEAYLG  V +AV+TVPAYF
Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAYF 148



 Score =  115 bits (276), Expect = 7e-27
 Identities = 52/60 (86%), Positives = 57/60 (95%)
 Frame = +2

Query: 74  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4   SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63



 Score = 74.5 bits (175), Expect = 1e-14
 Identities = 35/42 (83%), Positives = 38/42 (90%)
 Frame = +3

Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE 632
           +DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+   GE
Sbjct: 149 NDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGE 190


>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 644

 Score =  131 bits (316), Expect = 9e-32
 Identities = 59/88 (67%), Positives = 71/88 (80%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
           P   IFDAKRLIGR+F D  VQ+DMKHWPF+V+   GKP I+V +KGE KTF PEE+SSM
Sbjct: 61  PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120

Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
           VL KM+E+AEA+LG  V +AV+TVPAYF
Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAYF 148



 Score =  115 bits (276), Expect = 7e-27
 Identities = 52/60 (86%), Positives = 57/60 (95%)
 Frame = +2

Query: 74  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4   SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63



 Score = 71.3 bits (167), Expect = 1e-13
 Identities = 34/42 (80%), Positives = 37/42 (88%)
 Frame = +3

Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE 632
           +DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+    E
Sbjct: 149 NDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHE 190


>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 613

 Score =  118 bits (283), Expect = 9e-28
 Identities = 53/88 (60%), Positives = 67/88 (76%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
           P+  +FDAKRLIGR++ED   Q D+KHWPF+V+ + G P I+V Y GE K F  +E+S+M
Sbjct: 65  PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124

Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
           VLTKMKE +EA L K V+ AVITVPAYF
Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAYF 152



 Score =  102 bits (245), Expect = 4e-23
 Identities = 46/60 (76%), Positives = 52/60 (86%)
 Frame = +2

Query: 74  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           A+GIDLGTTYSCV V++   VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N
Sbjct: 8   AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67



 Score = 68.9 bits (161), Expect = 6e-13
 Identities = 33/37 (89%), Positives = 34/37 (91%)
 Frame = +3

Query: 510 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 620
           DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K
Sbjct: 154 DSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAK 190


>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 663

 Score =  113 bits (273), Expect = 2e-26
 Identities = 50/88 (56%), Positives = 69/88 (78%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
           P+  IFD KRLIGRKF++ T+  D+K +PF +V+D  +P ++V   G+ K F PEE+S+M
Sbjct: 94  PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153

Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
           +L+KMK+TAEAYLGK V +AV+TVPAYF
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYF 181



 Score =  100 bits (240), Expect = 2e-22
 Identities = 44/59 (74%), Positives = 51/59 (86%)
 Frame = +2

Query: 77  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ   NP N
Sbjct: 38  IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPEN 96



 Score = 72.9 bits (171), Expect = 3e-14
 Identities = 33/40 (82%), Positives = 38/40 (95%)
 Frame = +3

Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGT 626
           +D+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLDK  T
Sbjct: 182 NDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT 221


>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 674

 Score = 82.6 bits (195), Expect = 4e-17
 Identities = 38/88 (43%), Positives = 55/88 (62%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
           P+   F  KRLIGR+F++  VQ D+K  P+++V            +   KT+ P ++   
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166

Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
           +L+KM+ETA  YLGK V+NAV+TVPAYF
Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYF 194



 Score = 76.2 bits (179), Expect = 4e-15
 Identities = 35/66 (53%), Positives = 46/66 (69%), Gaps = 1/66 (1%)
 Frame = +2

Query: 65  KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 241
           K P +GIDLGTT SC+ + +    ++IAN +G RTTPS VAFT D ERL+G +AK Q  +
Sbjct: 49  KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108

Query: 242 NPNNKY 259
           NP N +
Sbjct: 109 NPENTF 114



 Score = 69.3 bits (162), Expect = 4e-13
 Identities = 31/38 (81%), Positives = 36/38 (94%)
 Frame = +3

Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 620
           +DSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK
Sbjct: 195 NDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK 232


>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
           Pss1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 52.0 bits (119), Expect = 7e-08
 Identities = 22/64 (34%), Positives = 40/64 (62%)
 Frame = +2

Query: 62  AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 241
           ++   VGID G + + + V ++  +++I N+  NR+TPS V++ +  R +G+AAK+  A 
Sbjct: 3   SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62

Query: 242 NPNN 253
           N  N
Sbjct: 63  NFRN 66



 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 25/85 (29%), Positives = 44/85 (51%), Gaps = 1/85 (1%)
 Frame = +1

Query: 268 KRLIGRKFEDATVQ-ADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 444
           KRL GR ++D  ++  +      ++    G    KV Y  E+  F   ++ +   TK+K 
Sbjct: 72  KRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKA 131

Query: 445 TAEAYLGKTVQNAVITVPAYFMTLK 519
            AEA L  +V + VI++PA+F  ++
Sbjct: 132 IAEAELIGSVSDVVISIPAWFTDIQ 156



 Score = 41.1 bits (92), Expect = 1e-04
 Identities = 18/36 (50%), Positives = 26/36 (72%)
 Frame = +3

Query: 510 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDK 617
           D QR+A  +A  I+GLN LR++N+  AAA+ YG+ K
Sbjct: 154 DIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITK 189


>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 566

 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 18/59 (30%), Positives = 33/59 (55%)
 Frame = +2

Query: 77  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           +GI  G   S +   + GK +++AN++GNR  PS +++   +   G  A+ Q+  N +N
Sbjct: 27  IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADN 85



 Score = 33.1 bits (72), Expect = 0.034
 Identities = 16/47 (34%), Positives = 25/47 (53%)
 Frame = +1

Query: 385 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDK 525
           ++K     E S   L ++ E+AE +LG  V   V++VP YF   + K
Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRK 180


>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 848

 Score = 29.9 bits (64), Expect = 0.32
 Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
 Frame = +2

Query: 77  VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           + ID GT ++   + + G  +EI+      R   S VAF   ER+ G  A N     P  
Sbjct: 25  LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82

Query: 254 KYSMPNV 274
            +S+ NV
Sbjct: 83  AHSIRNV 89



 Score = 26.2 bits (55), Expect = 3.9
 Identities = 19/94 (20%), Positives = 41/94 (43%), Gaps = 2/94 (2%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRK-FEDATVQADMKHWPFEVVSDGGKPKIKVAYK-GEDKTFFPEEVS 417
           P   I + K L+     E   VQ     +P   + +  +    +++   +++ +  EE+ 
Sbjct: 82  PAHSIRNVKELLDTAGLESVLVQKYQSSYPAIQLVENEETTSGISFVISDEENYSLEEII 141

Query: 418 SMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLK 519
           +M +      AE    + + + V+TVP +F  L+
Sbjct: 142 AMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQ 175


>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 29.5 bits (63), Expect = 0.42
 Identities = 26/137 (18%), Positives = 59/137 (43%), Gaps = 11/137 (8%)
 Frame = -3

Query: 614 VKTVSNRSSSRFIDDSENVQARDGTCIFCGLSLRVMKYAGTVITAFCTVLPR*ASAVSFI 435
           V ++ + S+S     S ++ +   +  F   S        + I++  T+L     + S +
Sbjct: 576 VSSILSSSTSSPSSTSLSISSSSTSSTFSSASTSSPSSISSSISSSSTILSSPTPSTSSL 635

Query: 434 FVSTMELTSSGKKVLSSPL-----------YATLILGLPPSLTTSKGQCFMSACTVASSN 288
            +S+  + S    +LSS +           Y++ ++    +L +S     +S+  VASS+
Sbjct: 636 MISSSSIISGSSSILSSSISTIPISSSLSTYSSSVIPSSSTLVSSSSSLIVSSSPVASSS 695

Query: 287 LRPMRRLASNICCWGSS 237
             P+   +S +  + +S
Sbjct: 696 SSPIPSSSSLVSTYSAS 712


>SPAC25B8.04c |||mitochondrial splicing suppressor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 378

 Score = 28.7 bits (61), Expect = 0.74
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = +3

Query: 69  HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 164
           HP   S W+P    LVS+    W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314


>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2244

 Score = 27.5 bits (58), Expect = 1.7
 Identities = 14/45 (31%), Positives = 24/45 (53%)
 Frame = +2

Query: 173  PSYVAFTDTERLIGDAAKNQVAMNPNNKYSMPNVSSDVSSKMLLC 307
            P  +A T  ER++  AAK   A+N    Y++  ++ D   K++ C
Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296


>SPAC2F7.03c |pom1||DYRK family protein kinase
           Pom1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1087

 Score = 25.4 bits (53), Expect = 6.9
 Identities = 17/66 (25%), Positives = 31/66 (46%)
 Frame = +1

Query: 55  QNGKSTRSRNRSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCRQEPG 234
           +NGK T +R +S     + W  P++E    R   G  D +    V R   +  +  +EP 
Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE----RSSNGNSDSASPPPVPRLSITRSQVSREP- 531

Query: 235 GDEPQQ 252
            ++P++
Sbjct: 532 -EKPEE 536


>SPAC823.11 |||sphingosine-1-phosphate phosphatase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 411

 Score = 25.4 bits (53), Expect = 6.9
 Identities = 11/35 (31%), Positives = 19/35 (54%)
 Frame = -3

Query: 155 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 51
           G W  SP S +  H   ++++  +F +RVL   C+
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310


>SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 330

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = -2

Query: 384 TLICYLDLRFASITDNLERPVLHVGLHSSIFELTSDETFGI 262
           T I  L   + S+TDN+ER  + +G  + I  +TS   F +
Sbjct: 142 TNIALLLATYPSVTDNIER-FIFMGGSTGIGNITSQAEFNV 181


>SPBC776.10c |cog6||Golgi transport complex peripheral subunit Cog6
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 675

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 10/28 (35%), Positives = 16/28 (57%)
 Frame = +2

Query: 218 AAKNQVAMNPNNKYSMPNVSSDVSSKML 301
           A K Q+ +NP   + +PNV  D  + +L
Sbjct: 323 ARKRQIQLNPFPPWDVPNVLEDQMNSLL 350


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,737,500
Number of Sequences: 5004
Number of extensions: 58329
Number of successful extensions: 219
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -