Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00095
(634 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 140 1e-33
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 138 3e-33
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 132 2e-31
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 118 4e-27
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 71 8e-13
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 68 7e-12
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 55 4e-08
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 6e-04
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 38 0.009
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.78
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.4
SB_47707| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.1
SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 4.1
SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08) 28 5.5
SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.5
SB_24803| Best HMM Match : LRR_1 (HMM E-Value=0.0066) 27 9.6
SB_24762| Best HMM Match : Astacin (HMM E-Value=0) 27 9.6
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 140 bits (338), Expect = 1e-33
Identities = 64/88 (72%), Positives = 76/88 (86%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ IFDAKRLIGR+++DATVQ+DMK WPF+++SD KPKI+V YKGE KTF EE+SSM
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
VL KMKETAEAYLG+ V +AVITVPAYF
Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPAYF 150
Score = 129 bits (312), Expect = 2e-30
Identities = 58/65 (89%), Positives = 62/65 (95%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 239 MNPNN 253
+NP N
Sbjct: 61 LNPEN 65
Score = 76.2 bits (179), Expect = 2e-14
Identities = 35/42 (83%), Positives = 40/42 (95%)
Frame = +3
Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE 632
+DSQRQATKDAGTI+GLNVLR+INEPTAAA+AYGLDK +GE
Sbjct: 151 NDSQRQATKDAGTIAGLNVLRVINEPTAAALAYGLDKSLSGE 192
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 138 bits (335), Expect = 3e-33
Identities = 61/88 (69%), Positives = 73/88 (82%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ +FDAKRLIGRKF+D V DM HWPF V+ +G +PKI+V +KGE K+FFPEE+SSM
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
VLTKMKETAEAYLG V +AV+TVPAYF
Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPAYF 150
Score = 134 bits (324), Expect = 6e-32
Identities = 62/65 (95%), Positives = 63/65 (96%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 239 MNPNN 253
MNP N
Sbjct: 61 MNPEN 65
Score = 79.0 bits (186), Expect = 3e-15
Identities = 37/38 (97%), Positives = 38/38 (100%)
Frame = +3
Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 620
HDSQRQATKDAGTI+GLNVLRIINEPTAAAIAYGLDKK
Sbjct: 151 HDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKK 188
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 132 bits (320), Expect = 2e-31
Identities = 60/65 (92%), Positives = 62/65 (95%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 239 MNPNN 253
MNP N
Sbjct: 61 MNPTN 65
Score = 114 bits (275), Expect = 5e-26
Identities = 64/113 (56%), Positives = 73/113 (64%), Gaps = 25/113 (22%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEV--- 414
P IFDAKRLIGR+F+D V+ DMKHW FEVV++ G+PK+KV YKGE KTFF EEV
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122
Query: 415 ----------------------SSMVLTKMKETAEAYLGKTVQNAVITVPAYF 507
SSMVL KMKETAEAYLG V +AV+TVPAYF
Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYF 175
Score = 75.4 bits (177), Expect = 4e-14
Identities = 36/38 (94%), Positives = 37/38 (97%)
Frame = +3
Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 620
+DSQRQATKDAG ISGLNVLRIINEPTAAAIAYGLDKK
Sbjct: 244 NDSQRQATKDAGVISGLNVLRIINEPTAAAIAYGLDKK 281
Score = 54.4 bits (125), Expect = 7e-08
Identities = 25/33 (75%), Positives = 29/33 (87%)
Frame = +1
Query: 409 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAYF 507
++SSMVL KMKETAEAYLG V +AV+TVPAYF
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYF 243
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 118 bits (284), Expect = 4e-27
Identities = 55/88 (62%), Positives = 69/88 (78%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ +FDAKRLIGR ++D +VQ D+K +PF+VV KP ++V K E KTF EE+S+M
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYF 507
VLTKMKETAEAYLGK V +AV+TVPAYF
Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPAYF 848
Score = 102 bits (244), Expect = 3e-22
Identities = 47/60 (78%), Positives = 53/60 (88%), Gaps = 1/60 (1%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNPNN 253
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763
Score = 68.9 bits (161), Expect = 3e-12
Identities = 31/38 (81%), Positives = 36/38 (94%)
Frame = +3
Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 620
+D+QRQATKDAG I+GL V+RIINEPTAAAIAYG+DKK
Sbjct: 849 NDAQRQATKDAGAIAGLTVMRIINEPTAAAIAYGMDKK 886
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 70.9 bits (166), Expect = 8e-13
Identities = 35/81 (43%), Positives = 51/81 (62%), Gaps = 1/81 (1%)
Frame = +1
Query: 268 KRLIGRKFEDATVQADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 444
KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V +M+ T++K
Sbjct: 127 KRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAMLFTRLKT 186
Query: 445 TAEAYLGKTVQNAVITVPAYF 507
TAE L V + VI+VP+Y+
Sbjct: 187 TAEIALKTKVTDCVISVPSYY 207
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 232
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
Score = 40.7 bits (91), Expect = 0.001
Identities = 17/37 (45%), Positives = 26/37 (70%)
Frame = +3
Query: 510 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 620
D QR+ DA +GLN LR++N+ TA ++AYG+ K+
Sbjct: 209 DRQRRCMLDASATAGLNCLRLMNDTTAVSLAYGIYKQ 245
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 67.7 bits (158), Expect = 7e-12
Identities = 30/65 (46%), Positives = 44/65 (67%), Gaps = 2/65 (3%)
Frame = +2
Query: 65 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 239 MNPNN 253
+NP N
Sbjct: 90 INPKN 94
Score = 58.4 bits (135), Expect = 4e-09
Identities = 37/105 (35%), Positives = 53/105 (50%), Gaps = 1/105 (0%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDG-GKPKIKVAYKGEDKTFFPEEVSS 420
P+ I+DAKR IG+ F + K + F V +D G V KG PEE+
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151
Query: 421 MVLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQKMQVPSLA 555
V+ K++ AE L TV AV++VPA F +K + ++ SLA
Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMSVPAEF-DIKQRNATVKAASLA 195
Score = 52.0 bits (119), Expect = 4e-07
Identities = 23/35 (65%), Positives = 29/35 (82%)
Frame = +3
Query: 516 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 620
QR AT A +++G+ VLR+INEPTAAA+AYGL KK
Sbjct: 184 QRNATVKAASLAGITVLRVINEPTAAALAYGLHKK 218
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 55.2 bits (127), Expect = 4e-08
Identities = 25/48 (52%), Positives = 37/48 (77%)
Frame = +1
Query: 364 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYF 507
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+F
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHF 49
Score = 48.8 bits (111), Expect = 4e-06
Identities = 34/66 (51%), Positives = 39/66 (59%), Gaps = 24/66 (36%)
Frame = +3
Query: 507 HDSQRQATKDAGTISGLNVL------------------------RIINEPTAAAIAYGLD 614
+D+QRQATKDAGTI+GL+VL RIINEPTAAAIAYGLD
Sbjct: 50 NDAQRQATKDAGTIAGLDVLSRTASERPPSRFRRRRPHGRSRVLRIINEPTAAAIAYGLD 109
Query: 615 KKGTGE 632
K G+
Sbjct: 110 KARGGK 115
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 41.5 bits (93), Expect = 6e-04
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +2
Query: 128 GKVEIIANDQGNRTTPSYVAFTDTERLI 211
G IIAND G+R TP+ VAF+DTE+LI
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLI 110
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 37.5 bits (83), Expect = 0.009
Identities = 17/50 (34%), Positives = 28/50 (56%)
Frame = +1
Query: 358 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYF 507
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP +F
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFF 116
Score = 34.7 bits (76), Expect = 0.063
Identities = 16/35 (45%), Positives = 24/35 (68%)
Frame = +3
Query: 507 HDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGL 611
+ ++R+A A + GLNVL+I+N TA A+ YGL
Sbjct: 117 NQAERRALLRAAELVGLNVLQIMNSNTAVALNYGL 151
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.78
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +2
Query: 137 EIIANDQGNRTTPSYVAFT-DTERL 208
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 2.4
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -3
Query: 245 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 147
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_47707| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 627
Score = 29.1 bits (62), Expect = 3.1
Identities = 24/72 (33%), Positives = 32/72 (44%), Gaps = 3/72 (4%)
Frame = +3
Query: 423 GAYENEG-NCRSLSWQNCAECSYHGSRVLHDSQRQAT--KDAGTISGLNVLRIINEPTAA 593
GA +N + SL W C + YHG +V+ + A AG S NV+R + P A
Sbjct: 512 GATQNANESLNSLVWARCPKHKYHGYKVIRCAVAAAVCHFHAGASSRENVMRRLCVP-AG 570
Query: 594 AIAYGLDKKGTG 629
G K TG
Sbjct: 571 HTQEGHPSKRTG 582
>SB_9053| Best HMM Match : TIG (HMM E-Value=0)
Length = 2990
Score = 28.7 bits (61), Expect = 4.1
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +2
Query: 5 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 91
HVC +V T K K ++K PA GI+L
Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228
>SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08)
Length = 1011
Score = 28.3 bits (60), Expect = 5.5
Identities = 17/62 (27%), Positives = 27/62 (43%)
Frame = +1
Query: 355 KPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQK 534
+PK K + E+ EE +M ++K LGK V+ + T +TL + Q
Sbjct: 654 QPKFKKTKEEEEFDKLLEEADAMTQQEVKNVKNIKLGKNVKTQLYTCEPCNITLNSQKQM 713
Query: 535 MQ 540
Q
Sbjct: 714 SQ 715
>SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1506
Score = 28.3 bits (60), Expect = 5.5
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = -3
Query: 452 SAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMSACTVASSNLRP 279
S F+F ST + +S +KV+ SPL + + P+ G S C V + + P
Sbjct: 1108 SPAKFVFTSTGIVRASTRKVVPSPLRRSKVPPTVPAQRLFNGDRVESICNVQNLSRCP 1165
>SB_24803| Best HMM Match : LRR_1 (HMM E-Value=0.0066)
Length = 727
Score = 27.5 bits (58), Expect = 9.6
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = -2
Query: 510 HEVRGNRDNCILHSFAKISFGSFLHFRKHHGTDFLGEKGFVFTLI 376
H GN+ +CIL F+K +F H R DF G +G T +
Sbjct: 19 HLDHGNQTDCILLDFSK-AFDRVAHGRLLQKLDFYGIRGKPLTWV 62
>SB_24762| Best HMM Match : Astacin (HMM E-Value=0)
Length = 733
Score = 27.5 bits (58), Expect = 9.6
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = -2
Query: 540 LHLLWLVFESHEVRGNRDNCILHSFAKIS 454
++ LWL F + + D C+LHS A ++
Sbjct: 415 IYTLWLDFSRYYSSASSDICVLHSIAHLA 443
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,466,385
Number of Sequences: 59808
Number of extensions: 490560
Number of successful extensions: 1508
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1409
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1501
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1584657875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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