BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00069 (738 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 64 2e-11 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 51 2e-07 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 39 9e-04 SPBC342.05 |crb2|rhp9, rhp9|DNA repair protein RAD9 homolog, Rhp... 32 0.074 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 31 0.17 SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|ch... 29 0.91 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 27 2.1 SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase Ran1|Schi... 27 2.8 SPAC1296.01c ||SPAC22F3.01|phosphoacetylglucosamine mutase |Schi... 27 3.7 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 4.9 SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|c... 26 4.9 SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 26 4.9 SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr... 26 6.4 SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces pombe... 26 6.4 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 64.1 bits (149), Expect = 2e-11 Identities = 30/42 (71%), Positives = 35/42 (83%) Frame = +1 Query: 1 VISMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKSQE 126 VISML+ PNDESPAN+DAAKE+RE EFKK+V R VR+S E Sbjct: 124 VISMLSSPNDESPANIDAAKEFRENPQEFKKRVRRLVRRSIE 165 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 50.8 bits (116), Expect = 2e-07 Identities = 23/40 (57%), Positives = 29/40 (72%) Frame = +1 Query: 1 VISMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKS 120 V+SMLA+PNDES AN+DA K WRE E+ + V R RK+ Sbjct: 124 VMSMLAEPNDESGANIDACKMWREDREEYCRVVRRLARKT 163 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 38.7 bits (86), Expect = 9e-04 Identities = 18/40 (45%), Positives = 26/40 (65%) Frame = +1 Query: 7 SMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKSQE 126 S+L DPN+ SPAN +AA+ RE E+ ++V + V S E Sbjct: 111 SLLNDPNNASPANAEAAQLHRENKKEYVRRVRKTVEDSWE 150 >SPBC342.05 |crb2|rhp9, rhp9|DNA repair protein RAD9 homolog, Rhp9|Schizosaccharomyces pombe|chr 2|||Manual Length = 778 Score = 32.3 bits (70), Expect = 0.074 Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 1/54 (1%) Frame = -2 Query: 461 TTSHQSLSQSKQI-MKKGTVIRNNPLGSFSHTVLLSF*TPNRILSTLSAKMKQN 303 T S + +Q K+ +KKG V+++ LG HTV+ +F + N LS ++ N Sbjct: 404 TMSTVNSNQIKRFFLKKGDVVQSTRLGKIKHTVVKTFRSTNEQLSLIAVDALNN 457 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 31.1 bits (67), Expect = 0.17 Identities = 13/40 (32%), Positives = 24/40 (60%) Frame = +1 Query: 7 SMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKSQE 126 S+L +PN+ SP N AA+ W + E+K+ + + ++ E Sbjct: 136 SLLGEPNNASPLNAQAAELWSKDPIEYKRLLMQRYKEIDE 175 >SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 548 Score = 28.7 bits (61), Expect = 0.91 Identities = 14/41 (34%), Positives = 23/41 (56%) Frame = +2 Query: 227 NVIHLASPYDPLTPNPHTSTADVFAYSVSFLQKEWRESYSE 349 +V+HL P D LTP PH D F + + + W+ ++S+ Sbjct: 53 SVLHLRGPSDHLTPQPH---VDSFGNVLCWNGEIWQINHSD 90 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 27.5 bits (58), Expect = 2.1 Identities = 14/36 (38%), Positives = 20/36 (55%) Frame = +1 Query: 1 VISMLADPNDESPANVDAAKEWRERYSEFKKKVARC 108 +IS+L++ + SP NVDAAK R + RC Sbjct: 111 IISLLSNYDASSPLNVDAAKLLRTGDKTAYNSLVRC 146 >SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase Ran1|Schizosaccharomyces pombe|chr 2|||Manual Length = 470 Score = 27.1 bits (57), Expect = 2.8 Identities = 9/21 (42%), Positives = 14/21 (66%) Frame = +2 Query: 242 ASPYDPLTPNPHTSTADVFAY 304 A+ Y P+TP P+ + A +F Y Sbjct: 415 ANSYMPITPTPYPNNAKIFGY 435 >SPAC1296.01c ||SPAC22F3.01|phosphoacetylglucosamine mutase |Schizosaccharomyces pombe|chr 1|||Manual Length = 542 Score = 26.6 bits (56), Expect = 3.7 Identities = 18/63 (28%), Positives = 29/63 (46%), Gaps = 1/63 (1%) Frame = +2 Query: 188 IVTPANI-LLQTLKNVIHLASPYDPLTPNPHTSTADVFAYSVSFLQKEWRESYSEFKRKV 364 +++PA L+TLK V L + D + A+ L KEW + YSE ++ Sbjct: 407 VLSPAQFNALKTLKTVFELINQTDGDAITNLLLVEVILAHKNCTL-KEWNQLYSEIPSRL 465 Query: 365 AQC 373 +C Sbjct: 466 IRC 468 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 26.2 bits (55), Expect = 4.9 Identities = 12/37 (32%), Positives = 22/37 (59%) Frame = +1 Query: 10 MLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKS 120 +L DPN SPA +A +++ E++K+V R++ Sbjct: 119 LLDDPNIASPAQTEAYTMFKKDKVEYEKRVRAQAREN 155 >SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1717 Score = 26.2 bits (55), Expect = 4.9 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = -2 Query: 470 SVLTTSHQSLSQSKQIMKKGTVIRNNPLG 384 ++ T S +SKQ +K+ T ++N+P G Sbjct: 497 TIFTQGPNSTKESKQAVKEETSVKNSPKG 525 >SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Schizosaccharomyces pombe|chr 1|||Manual Length = 738 Score = 26.2 bits (55), Expect = 4.9 Identities = 15/47 (31%), Positives = 22/47 (46%) Frame = +2 Query: 2 SFQCSLIQMMRVPLMLMLQKNGENDIRNSKRKLPDVLGKVKKIASRS 142 S Q + MR+P + NS +K D L K++K+ SRS Sbjct: 331 SIQSRAFKKMRLPKISRTTDTPPASTSNSNKKNLDKLKKMRKLCSRS 377 >SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr 2|||Manual Length = 1471 Score = 25.8 bits (54), Expect = 6.4 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = +2 Query: 14 SLIQMMRVPLMLMLQKNGENDI 79 SL+ +M+ PL+ L+KN ND+ Sbjct: 1351 SLLPLMQSPLVFCLRKNDANDL 1372 >SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces pombe|chr 2|||Manual Length = 527 Score = 25.8 bits (54), Expect = 6.4 Identities = 18/46 (39%), Positives = 24/46 (52%) Frame = -3 Query: 601 KKDEKKTFNSILFIKYGSDTISLL*VFILNQLNIVSGPGGGVRSAS 464 KKDEKK N + +I +SL +F N +IV G G + AS Sbjct: 313 KKDEKKALNLLAWILKLYSYMSLFILFGSNYSDIVLLFGAGSKWAS 358 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,183,627 Number of Sequences: 5004 Number of extensions: 68576 Number of successful extensions: 216 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 199 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 216 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 349251756 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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