BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00069 (738 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g46460.1 68416.m05037 ubiquitin-conjugating enzyme 13 (UBC13)... 78 7e-15 At5g59300.1 68418.m07430 ubiquitin-conjugating enzyme 7 (UBC7) E... 77 2e-14 At3g55380.1 68416.m06151 ubiquitin-conjugating enzyme 14 (UBC14)... 74 1e-13 At5g62540.1 68418.m07849 ubiquitin-conjugating enzyme 3 (UBC3) E... 40 0.002 At2g02760.1 68415.m00219 ubiquitin-conjugating enzyme 2 (UBC2) E... 37 0.016 At1g14400.2 68414.m01708 ubiquitin-conjugating enzyme 1 (UBC1) E... 36 0.021 At1g14400.1 68414.m01707 ubiquitin-conjugating enzyme 1 (UBC1) E... 36 0.021 At1g13920.1 68414.m01633 remorin family protein contains Pfam do... 35 0.065 At3g20520.1 68416.m02598 glycerophosphoryl diester phosphodieste... 29 2.4 At1g50490.1 68414.m05662 ubiquitin-conjugating enzyme 20 (UBC20)... 29 2.4 At1g50750.1 68414.m05707 expressed protein 29 3.2 At3g20060.1 68416.m02537 ubiquitin-conjugating enzyme 19 (UBC19)... 28 5.6 At1g64790.1 68414.m07346 translational activator family protein ... 27 9.8 At1g48490.1 68414.m05420 protein kinase, putative similar to inc... 27 9.8 >At3g46460.1 68416.m05037 ubiquitin-conjugating enzyme 13 (UBC13) E2; identical to gi:992706 Length = 166 Score = 77.8 bits (183), Expect = 7e-15 Identities = 34/42 (80%), Positives = 39/42 (92%) Frame = +1 Query: 1 VISMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKSQE 126 +ISML+ PNDESPANV+AAKEWRE+ EFKKKV+RCVRKSQE Sbjct: 123 IISMLSGPNDESPANVEAAKEWREKRDEFKKKVSRCVRKSQE 164 Score = 42.3 bits (95), Expect = 3e-04 Identities = 17/23 (73%), Positives = 20/23 (86%) Frame = +2 Query: 323 KEWRESYSEFKRKVAQCVRKSQE 391 KEWRE EFK+KV++CVRKSQE Sbjct: 142 KEWREKRDEFKKKVSRCVRKSQE 164 >At5g59300.1 68418.m07430 ubiquitin-conjugating enzyme 7 (UBC7) E2; identical to gi:992703, SP:P42747 Length = 198 Score = 76.6 bits (180), Expect = 2e-14 Identities = 33/42 (78%), Positives = 39/42 (92%) Frame = +1 Query: 1 VISMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKSQE 126 +ISML+ PNDESPANV+AAKEWR++ EFKKKV+RCVRKSQE Sbjct: 155 IISMLSGPNDESPANVEAAKEWRDKRDEFKKKVSRCVRKSQE 196 Score = 41.1 bits (92), Expect = 7e-04 Identities = 16/23 (69%), Positives = 20/23 (86%) Frame = +2 Query: 323 KEWRESYSEFKRKVAQCVRKSQE 391 KEWR+ EFK+KV++CVRKSQE Sbjct: 174 KEWRDKRDEFKKKVSRCVRKSQE 196 >At3g55380.1 68416.m06151 ubiquitin-conjugating enzyme 14 (UBC14) E2; UbcAT3; identical to gi:2129757, S46656 Length = 167 Score = 73.7 bits (173), Expect = 1e-13 Identities = 31/42 (73%), Positives = 39/42 (92%) Frame = +1 Query: 1 VISMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKSQE 126 +ISML+ PNDESPANV+AAKEWR+ +EF+KKV+RCVR+SQE Sbjct: 124 IISMLSGPNDESPANVEAAKEWRDNRAEFRKKVSRCVRRSQE 165 Score = 39.5 bits (88), Expect = 0.002 Identities = 14/23 (60%), Positives = 22/23 (95%) Frame = +2 Query: 323 KEWRESYSEFKRKVAQCVRKSQE 391 KEWR++ +EF++KV++CVR+SQE Sbjct: 143 KEWRDNRAEFRKKVSRCVRRSQE 165 >At5g62540.1 68418.m07849 ubiquitin-conjugating enzyme 3 (UBC3) E2; identical to gi:431261, SP:P42746 Length = 150 Score = 39.5 bits (88), Expect = 0.002 Identities = 17/38 (44%), Positives = 25/38 (65%) Frame = +1 Query: 7 SMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKS 120 S+L DPN +SPAN +AA+ + E E+ +KV V +S Sbjct: 111 SLLCDPNPDSPANAEAARLFSENKREYNRKVIEIVEQS 148 >At2g02760.1 68415.m00219 ubiquitin-conjugating enzyme 2 (UBC2) E2; identical to gi:2689242, SP:P42745 Length = 152 Score = 36.7 bits (81), Expect = 0.016 Identities = 16/38 (42%), Positives = 24/38 (63%) Frame = +1 Query: 7 SMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKS 120 S+L DPN SPAN +AA+ + E E+ ++V V +S Sbjct: 111 SLLCDPNPNSPANSEAARMFSESKREYNRRVREVVEQS 148 >At1g14400.2 68414.m01708 ubiquitin-conjugating enzyme 1 (UBC1) E2; identical to gi:431259, SP:P25865 Length = 152 Score = 36.3 bits (80), Expect = 0.021 Identities = 16/38 (42%), Positives = 24/38 (63%) Frame = +1 Query: 7 SMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKS 120 S+L DPN SPAN +AA+ + E E+ ++V V +S Sbjct: 111 SLLCDPNPNSPANSEAARMYSESKREYNRRVRDVVEQS 148 >At1g14400.1 68414.m01707 ubiquitin-conjugating enzyme 1 (UBC1) E2; identical to gi:431259, SP:P25865 Length = 152 Score = 36.3 bits (80), Expect = 0.021 Identities = 16/38 (42%), Positives = 24/38 (63%) Frame = +1 Query: 7 SMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKS 120 S+L DPN SPAN +AA+ + E E+ ++V V +S Sbjct: 111 SLLCDPNPNSPANSEAARMYSESKREYNRRVRDVVEQS 148 >At1g13920.1 68414.m01633 remorin family protein contains Pfam domain, PF03763: Remorin, C-terminal region Length = 345 Score = 34.7 bits (76), Expect = 0.065 Identities = 22/91 (24%), Positives = 45/91 (49%), Gaps = 2/91 (2%) Frame = +2 Query: 77 IRNSKRKLPDVLGKVKKIAS--RSKRIFRKEYIYC*KRAIVTPANILLQTLKNVIHLASP 250 I+ ++RK P K++++ S R K++ ++ + K P+N L + + S Sbjct: 5 IKQTRRKHPASQEKIREVGSSTREKKVSARKSVSF-KEDKKKPSNWLQKQFSRQMSGQS- 62 Query: 251 YDPLTPNPHTSTADVFAYSVSFLQKEWRESY 343 YDP+ H + AY+++ ++ W E+Y Sbjct: 63 YDPIGEMDHAAAVAATAYAIATFEETWLENY 93 >At3g20520.1 68416.m02598 glycerophosphoryl diester phosphodiesterase family protein contains Pfam PF03009 : Glycerophosphoryl diester phosphodiesterase family; similar to glycerophosphodiester phosphodiesterase (GI:1399038) [Borrelia hermsii] Length = 729 Score = 29.5 bits (63), Expect = 2.4 Identities = 15/33 (45%), Positives = 20/33 (60%) Frame = +1 Query: 385 PRGLFLITVPFFIICLDCESDW*DVVRTLSGRP 483 PR +FLI + FFI+ S W +TLSG+P Sbjct: 4 PRVIFLILITFFILQTAFSSSW----QTLSGKP 32 >At1g50490.1 68414.m05662 ubiquitin-conjugating enzyme 20 (UBC20) nearly identical to ubiquitin-conjugating enzyme UBC20 [Arabidopsis thaliana] GI:22530867; contains Pfam profile PF00179: Ubiquitin-conjugating enzyme Length = 180 Score = 29.5 bits (63), Expect = 2.4 Identities = 13/33 (39%), Positives = 20/33 (60%) Frame = +1 Query: 7 SMLADPNDESPANVDAAKEWRERYSEFKKKVAR 105 S+L +PN SP N AA+ W + E++K V + Sbjct: 142 SLLGEPNISSPLNTQAAQLWSNQ-EEYRKMVEK 173 >At1g50750.1 68414.m05707 expressed protein Length = 816 Score = 29.1 bits (62), Expect = 3.2 Identities = 15/60 (25%), Positives = 30/60 (50%) Frame = +2 Query: 29 MRVPLMLMLQKNGENDIRNSKRKLPDVLGKVKKIASRSKRIFRKEYIYC*KRAIVTPANI 208 + + L L K +D+RNS R + LGK+ ++ + + + + + + IV P+ I Sbjct: 510 LSIAQFLRLNKKKYSDVRNSGRDASEPLGKISRVEADNNDLGTFQKLASIRDEIVPPSEI 569 >At3g20060.1 68416.m02537 ubiquitin-conjugating enzyme 19 (UBC19) nearly identical to ubiquitin-conjugating enzyme UBC19 [Arabidopsis thaliana] GI:22530865; contains Pfam profile PF00179: Ubiquitin-conjugating enzyme Length = 181 Score = 28.3 bits (60), Expect = 5.6 Identities = 13/33 (39%), Positives = 20/33 (60%) Frame = +1 Query: 7 SMLADPNDESPANVDAAKEWRERYSEFKKKVAR 105 S+L +PN SP N AA+ W + E++K V + Sbjct: 143 SLLGEPNISSPLNNQAAQLWSNQ-EEYRKMVEK 174 >At1g64790.1 68414.m07346 translational activator family protein similar to HsGCN1 [Homo sapiens] GI:2282576 Length = 2440 Score = 27.5 bits (58), Expect = 9.8 Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 2/44 (4%) Frame = -2 Query: 467 VLTTSHQSLSQSKQI--MKKGTVIRNNPLGSFSHTVLLSF*TPN 342 VLT +H + + Q+ + G+VI+N + S T+LL+ PN Sbjct: 1319 VLTDTHPKVQSAGQLALQQVGSVIKNPEISSLVPTLLLALTDPN 1362 >At1g48490.1 68414.m05420 protein kinase, putative similar to incomplete root hair elongation (IRE) [Arabidopsis thaliana] gi|6729346|dbj|BAA89783 Length = 878 Score = 27.5 bits (58), Expect = 9.8 Identities = 16/48 (33%), Positives = 22/48 (45%), Gaps = 1/48 (2%) Frame = +1 Query: 25 NDESPANVDAAKEWRERYSEFKKKVAR-CVRKSQEDCF*EQKDI*KRI 165 + ESPAN+D W+ S K V V K Q D E + K++ Sbjct: 97 DSESPANIDKMSSWKRLPSPASKGVQEAAVSKEQNDRKVEPPQVVKKL 144 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,110,441 Number of Sequences: 28952 Number of extensions: 340425 Number of successful extensions: 858 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 822 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 858 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1624036432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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