Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00063
(768 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protei... 77 2e-14
At1g04520.1 68414.m00443 33 kDa secretory protein-related contai... 33 0.16
At3g04170.1 68416.m00441 germin-like protein, putative contains ... 31 0.64
At1g64410.1 68414.m07301 hypothetical protein 31 0.85
At1g52960.1 68414.m05990 hypothetical protein very low similarit... 31 0.85
At4g04910.1 68417.m00714 AAA-type ATPase family protein similar ... 29 4.5
At5g61100.1 68418.m07666 hypothetical protein 28 6.0
At4g36540.2 68417.m05189 basic helix-loop-helix (bHLH) family pr... 28 6.0
At4g36540.1 68417.m05188 basic helix-loop-helix (bHLH) family pr... 28 6.0
At1g11780.1 68414.m01352 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 6.0
>At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protein
similar to SP|P12048 Bifunctional purine biosynthesis
protein purH [Includes:
Phosphoribosylaminoimidazolecarboxamide
formyltransferase (EC 2.1.2.3) (AICAR transformylase);
IMP cyclohydrolase (EC 3.5.4.10) (Inosinicase) (IMP
synthetase) (ATIC)] {Bacillus subtilis}; contains Pfam
profiles PF01808: AICARFT/IMPCHase bienzyme, PF02142:
MGS-like domain
Length = 596
Score = 76.6 bits (180), Expect = 2e-14
Identities = 35/63 (55%), Positives = 44/63 (69%)
Frame = +3
Query: 6 LLSLAKSLSECGLQLIASGGTATALRNAGLTVQDVSDITRAPEMLGGRVKTLHPAVHAGI 185
L SL L E G ++++GGTA+ L NAG++V V +T PEML GRVKTLHP +H GI
Sbjct: 84 LASLGNGLQELGYTIVSTGGTASTLENAGVSVTKVEKLTHFPEMLDGRVKTLHPNIHGGI 143
Query: 186 LAR 194
LAR
Sbjct: 144 LAR 146
Score = 40.7 bits (91), Expect = 0.001
Identities = 18/36 (50%), Positives = 25/36 (69%), Gaps = 1/36 (2%)
Frame = +2
Query: 254 VCNLYPFVQTVSKPD-VTVADAVENIDIGGVTLLRA 358
V NLYPF + V+ P ++ D +ENIDIGG ++RA
Sbjct: 168 VVNLYPFYEKVTAPGGISFEDGIENIDIGGPAMIRA 203
Score = 35.9 bits (79), Expect = 0.030
Identities = 19/48 (39%), Positives = 27/48 (56%)
Frame = +1
Query: 361 AKNHDRVTVVCDPADYDVVVKEIKENKHHQTSLGTRQRLALKAFTHTS 504
AKNH V +V D DY V++ +K + Q R++LA KAF H +
Sbjct: 205 AKNHKDVLIVVDSGDYQAVLEYLKGGQSDQQ---FRRKLAWKAFQHVA 249
>At1g04520.1 68414.m00443 33 kDa secretory protein-related contains
Pfam PF01657: Domain of unknown function, duplicated in
33 KDa secretory proteins
Length = 307
Score = 33.5 bits (73), Expect = 0.16
Identities = 17/54 (31%), Positives = 30/54 (55%)
Frame = -1
Query: 450 LMMFVLFDFFDYNIVVGRVTDDGDPVVVLGCARRRVTPPMSMFSTASATVTSGL 289
+MM +LF D N+VV T + ++ GCAR++ + P ++S A + + L
Sbjct: 12 MMMCLLFP--DLNVVVKSATTEYTTLIYKGCARQQFSDPSGLYSQALSAMFGSL 63
>At3g04170.1 68416.m00441 germin-like protein, putative contains
Pfam profile: PF01072 germin family; similar to
germin-like protein type2 GB:CAA63023 [SP|P92996], GLP6
[SP|P92997], GLP2A [SP|P92999] [Arabidopsis thaliana]
Length = 227
Score = 31.5 bits (68), Expect = 0.64
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +3
Query: 453 VFGHKAEISPEGVHSYFGLDLAISDYFRKQYSP 551
VFG K I P+ + F LD+ I Y RK +SP
Sbjct: 188 VFGSKPPIDPKILAKAFALDINIVRYLRKVFSP 220
>At1g64410.1 68414.m07301 hypothetical protein
Length = 1231
Score = 31.1 bits (67), Expect = 0.85
Identities = 21/56 (37%), Positives = 24/56 (42%)
Frame = +1
Query: 364 KNHDRVTVVCDPADYDVVVKEIKENKHHQTSLGTRQRLALKAFTHTSDLTSPYRTT 531
K HDRVTV +P D D KE E K + R A +A YRTT
Sbjct: 582 KGHDRVTVTVEPNDQDTAKKEKDEVKDY---FDCRYVSACEAMWRIFKFPIHYRTT 634
>At1g52960.1 68414.m05990 hypothetical protein very low similarity
to SP|Q9UUA2 DNA repair and recombination protein pif1,
mitochondrial precursor {Schizosaccharomyces pombe}
Length = 996
Score = 31.1 bits (67), Expect = 0.85
Identities = 21/56 (37%), Positives = 24/56 (42%)
Frame = +1
Query: 364 KNHDRVTVVCDPADYDVVVKEIKENKHHQTSLGTRQRLALKAFTHTSDLTSPYRTT 531
K HDRVTV +P D D KE E K + R A +A YRTT
Sbjct: 208 KGHDRVTVTVEPNDQDTAKKEKDEVKDY---FDCRYVSACEAMWRIFKFPIHYRTT 260
>At4g04910.1 68417.m00714 AAA-type ATPase family protein similar to
SP|P18708 Vesicular-fusion protein NSF
(N-ethylmaleimide-sensitive fusion protein)
(NEM-sensitive fusion protein) {Cricetulus griseus};
contains Pfam profiles PF00004: ATPase AAA family,
PF02359: Cell division protein 48 (CDC48) N-terminal
domain; contains non-consensus AT-AC splice sites at
intron 2
Length = 742
Score = 28.7 bits (61), Expect = 4.5
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = +3
Query: 426 NQREQTSSDVFGHKAEISPEGVHSYFGLDLAISDYFRKQYS 548
NQRE SS++F HK E + E + GL +D FR+ ++
Sbjct: 195 NQREAASSNIFKHK-EFNLESL-GIGGLGAEFADIFRRAFA 233
>At5g61100.1 68418.m07666 hypothetical protein
Length = 227
Score = 28.3 bits (60), Expect = 6.0
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = +1
Query: 106 MCRTSREHRRCSEVG*KLYIQRYTLVS*LDYRTLTRKT*NVRSTR 240
MCR +REH C+ V + + + D+ ++ KT N +S+R
Sbjct: 51 MCRDTREHTYCARVMFQRVPRLWICEECRDFSSVANKTANAQSSR 95
>At4g36540.2 68417.m05189 basic helix-loop-helix (bHLH) family
protein contains Pfam domain, PF00010: Helix-loop-helix
DNA-binding domain
Length = 302
Score = 28.3 bits (60), Expect = 6.0
Identities = 14/36 (38%), Positives = 18/36 (50%)
Frame = +1
Query: 436 NKHHQTSLGTRQRLALKAFTHTSDLTSPYRTTSASN 543
N H TSLG + + TH+ L + RT S SN
Sbjct: 262 NSDHTTSLGAKDHTSSSWETHSQCLYNSLRTDSVSN 297
>At4g36540.1 68417.m05188 basic helix-loop-helix (bHLH) family
protein contains Pfam domain, PF00010: Helix-loop-helix
DNA-binding domain
Length = 304
Score = 28.3 bits (60), Expect = 6.0
Identities = 14/36 (38%), Positives = 18/36 (50%)
Frame = +1
Query: 436 NKHHQTSLGTRQRLALKAFTHTSDLTSPYRTTSASN 543
N H TSLG + + TH+ L + RT S SN
Sbjct: 264 NSDHTTSLGAKDHTSSSWETHSQCLYNSLRTDSVSN 299
>At1g11780.1 68414.m01352 oxidoreductase, 2OG-Fe(II) oxygenase
family protein low similarity to alkB proteins from Homo
sapiens [SP|Q13686], Caulobacter crescentus
[GI:2055386]; contains Pfam profile PF03171:
oxidoreductase, 2OG-Fe(II) oxygenase family
Length = 345
Score = 28.3 bits (60), Expect = 6.0
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +3
Query: 624 DSLPITTLNGAPGFINLCDALNAWQLVKELKEAL 725
DS P+ ++ PGF + DAL+ + K +KE+L
Sbjct: 74 DSSPVFCIDNRPGFYFIPDALSLKEQCKWIKESL 107
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,390,695
Number of Sequences: 28952
Number of extensions: 309236
Number of successful extensions: 1028
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1006
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1027
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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