BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00053 (546 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 148 6e-37 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 147 1e-36 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 116 2e-27 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 81 1e-16 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 71 1e-13 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 67 1e-12 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 59 4e-10 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 58 8e-10 SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 30 0.26 SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosacchar... 29 0.34 SPAC3F10.05c |mug113||DUF1766 family protein|Schizosaccharomyces... 27 1.4 SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr ... 27 2.4 SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces ... 26 3.2 SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc... 26 4.2 SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 25 5.5 SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pm... 25 5.5 SPCC74.04 |||amino acid permease, unknown 15|Schizosaccharomyces... 25 7.3 SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 25 9.6 SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce... 25 9.6 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 148 bits (358), Expect = 6e-37 Identities = 71/83 (85%), Positives = 76/83 (91%) Frame = +3 Query: 3 MVNHFVQEFKRKYKKDLATNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTS 182 +VNHF+QEFKRK KKD+ N RA+RRLRTACERAKRTLSSS QASIEIDSLFEGIDFYTS Sbjct: 235 LVNHFIQEFKRKNKKDITGNARAVRRLRTACERAKRTLSSSAQASIEIDSLFEGIDFYTS 294 Query: 183 ITRARFEELNADLFRSTMEPVEK 251 ITRARFEEL ADLFR TMEPVE+ Sbjct: 295 ITRARFEELCADLFRKTMEPVER 317 Score = 104 bits (249), Expect = 1e-23 Identities = 51/81 (62%), Positives = 60/81 (74%) Frame = +2 Query: 251 VFRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNXSINPDEXXXXXXXXXXXI 430 V RD+K+DKA +++IVLVGGSTRIP+VQKL+ DFFNGKE SINPDE + Sbjct: 318 VLRDSKVDKASVNEIVLVGGSTRIPRVQKLVSDFFNGKEPCKSINPDEAVAYGAAVQAAV 377 Query: 431 LHGDKSEEVQDLLLLDVTPLS 493 L GD SE+ QDLLLLDV PLS Sbjct: 378 LTGDTSEKTQDLLLLDVAPLS 398 Score = 25.8 bits (54), Expect = 4.2 Identities = 11/12 (91%), Positives = 11/12 (91%) Frame = +3 Query: 510 GGVMTTLIKRNT 545 GGVMT LIKRNT Sbjct: 405 GGVMTPLIKRNT 416 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 147 bits (356), Expect = 1e-36 Identities = 71/83 (85%), Positives = 76/83 (91%) Frame = +3 Query: 3 MVNHFVQEFKRKYKKDLATNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTS 182 +VNHF QEFKRK KKD+ N RA+RRLRTACERAKRTLSSS QASIEIDSL+EGIDFYTS Sbjct: 235 LVNHFAQEFKRKNKKDITGNARAVRRLRTACERAKRTLSSSAQASIEIDSLYEGIDFYTS 294 Query: 183 ITRARFEELNADLFRSTMEPVEK 251 ITRARFEEL ADLFR+TMEPVEK Sbjct: 295 ITRARFEELCADLFRNTMEPVEK 317 Score = 103 bits (246), Expect = 2e-23 Identities = 50/82 (60%), Positives = 61/82 (74%) Frame = +2 Query: 248 EVFRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNXSINPDEXXXXXXXXXXX 427 +V RD+K+DK+ +++IVLVGGSTRIP++QKL+ DFFNGKE SINPDE Sbjct: 317 KVLRDSKIDKSSVNEIVLVGGSTRIPRIQKLVSDFFNGKEPCKSINPDEAVAYGAAVQAA 376 Query: 428 ILHGDKSEEVQDLLLLDVTPLS 493 IL GD SE+ QDLLLLDV PLS Sbjct: 377 ILVGDTSEKTQDLLLLDVAPLS 398 Score = 25.8 bits (54), Expect = 4.2 Identities = 11/12 (91%), Positives = 11/12 (91%) Frame = +3 Query: 510 GGVMTTLIKRNT 545 GGVMT LIKRNT Sbjct: 405 GGVMTPLIKRNT 416 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 116 bits (280), Expect = 2e-27 Identities = 53/83 (63%), Positives = 69/83 (83%) Frame = +3 Query: 3 MVNHFVQEFKRKYKKDLATNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTS 182 +V HF+QEFKRK K D++ + RALRRLR+ACERAKR LSS TQ ++E+DSL GIDF +S Sbjct: 239 LVEHFIQEFKRKQKIDISDDPRALRRLRSACERAKRALSSVTQTTVEVDSLSNGIDFSSS 298 Query: 183 ITRARFEELNADLFRSTMEPVEK 251 ITRARFE++NA F++T++PV K Sbjct: 299 ITRARFEDINATTFKATIDPVAK 321 Score = 95.1 bits (226), Expect = 6e-21 Identities = 47/83 (56%), Positives = 60/83 (72%), Gaps = 1/83 (1%) Frame = +2 Query: 248 EVFRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNXSINPDEXXXXXXXXXXX 427 +V +D+K+ KA +HDIVLVGGSTRIPKVQ+L+ DFF+G+ LN SINPDE Sbjct: 321 KVLKDSKVPKADVHDIVLVGGSTRIPKVQRLVSDFFDGRALNKSINPDEAVAYGAAVQAA 380 Query: 428 IL-HGDKSEEVQDLLLLDVTPLS 493 +L + S++ QDLLLLDV PLS Sbjct: 381 VLTNKADSDKTQDLLLLDVVPLS 403 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 81.0 bits (191), Expect = 1e-16 Identities = 35/83 (42%), Positives = 57/83 (68%) Frame = +3 Query: 3 MVNHFVQEFKRKYKKDLATNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTS 182 ++N+ + + RK D+ + +A+ +L+ E+AKRTLSS IEI+S F G DF + Sbjct: 267 VINYLARTYNRKNNVDVTKDLKAMGKLKREVEKAKRTLSSQKSVRIEIESFFNGQDFSET 326 Query: 183 ITRARFEELNADLFRSTMEPVEK 251 ++RA+FEE+N DLF+ T++PVE+ Sbjct: 327 LSRAKFEEINMDLFKKTLKPVEQ 349 Score = 75.4 bits (177), Expect = 5e-15 Identities = 38/82 (46%), Positives = 56/82 (68%) Frame = +2 Query: 248 EVFRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNXSINPDEXXXXXXXXXXX 427 +V +D+ + K++I DIVLVGGSTRIPKVQ+LL+ FF GK+ + INPDE Sbjct: 349 QVLKDSNLKKSEIDDIVLVGGSTRIPKVQELLESFF-GKKASKGINPDEAVAYGAAVQAG 407 Query: 428 ILHGDKSEEVQDLLLLDVTPLS 493 +L G++ + +++LLDV PL+ Sbjct: 408 VLSGEEGSD--NIVLLDVIPLT 427 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 70.5 bits (165), Expect = 1e-13 Identities = 40/92 (43%), Positives = 57/92 (61%), Gaps = 4/92 (4%) Frame = +3 Query: 3 MVNHFVQEFKRKYKKDLATNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTS 182 +VN F +EF++K D N R+L +LR CE KR LS+ T AS +DSL +GIDF++S Sbjct: 261 LVNFFAKEFEKKNGIDPRKNARSLAKLRAQCEITKRVLSNGTTASAAVDSLADGIDFHSS 320 Query: 183 ITRARFE-ELNADLFRS---TMEPVEKSSVMP 266 I R R++ +A L R E VEK+++ P Sbjct: 321 INRLRYDLAASATLNRMADLVTEAVEKANMEP 352 Score = 30.3 bits (65), Expect = 0.19 Identities = 11/36 (30%), Positives = 20/36 (55%) Frame = +2 Query: 248 EVFRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFF 355 E A M+ I +++L GG++ PK+ L++ F Sbjct: 343 EAVEKANMEPFDISEVILAGGASNTPKLTSLMESIF 378 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 67.3 bits (157), Expect = 1e-12 Identities = 32/84 (38%), Positives = 50/84 (59%) Frame = +3 Query: 3 MVNHFVQEFKRKYKKDLATNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTS 182 ++++F EFK KYK D+ +N +A RL TA ER K+ LS++ A + ++ + ID + Sbjct: 243 LIDYFAAEFKEKYKIDVLSNPKATFRLATAVERLKKVLSANANAPLNVEMIMNDIDASSF 302 Query: 183 ITRARFEELNADLFRSTMEPVEKS 254 I R+ FEEL L P+EK+ Sbjct: 303 IKRSDFEELIKPLLERLTPPIEKA 326 Score = 38.3 bits (85), Expect = 7e-04 Identities = 17/44 (38%), Positives = 31/44 (70%) Frame = +2 Query: 263 AKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNXSINPDE 394 A + K ++ I +VGG TR+P V++++ ++F GK L+ ++N DE Sbjct: 330 AGIKKEDLYSIEMVGGCTRVPIVKEVIANYF-GKGLSFTLNQDE 372 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 59.3 bits (137), Expect = 4e-10 Identities = 31/79 (39%), Positives = 49/79 (62%) Frame = +2 Query: 257 RDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNXSINPDEXXXXXXXXXXXILH 436 +DA + ++I++++LVGG TR+P+V + ++ F +E S+NPDE +L Sbjct: 368 KDANLQTSEINEVILVGGMTRMPRVVETVKSIFK-REPAKSVNPDEAVAIGAAIQGGVLS 426 Query: 437 GDKSEEVQDLLLLDVTPLS 493 G V+DL+LLDVTPLS Sbjct: 427 G----HVKDLVLLDVTPLS 441 Score = 48.8 bits (111), Expect = 5e-07 Identities = 28/88 (31%), Positives = 50/88 (56%), Gaps = 4/88 (4%) Frame = +3 Query: 3 MVNHFVQEFKRKYKKDLATNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGI----D 170 +V H V+ FK+ DL+ ++ A++R+R A E+AK LSS ++ I + + Sbjct: 279 LVRHIVETFKKNEGLDLSKDRLAVQRIREAAEKAKCELSSLSKTDISLPFITADATGPKH 338 Query: 171 FYTSITRARFEELNADLFRSTMEPVEKS 254 I+RA+FE+L L R T++P +++ Sbjct: 339 INMEISRAQFEKLVDPLVRRTIDPCKRA 366 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 58.0 bits (134), Expect = 8e-10 Identities = 30/84 (35%), Positives = 47/84 (55%) Frame = +3 Query: 3 MVNHFVQEFKRKYKKDLATNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTS 182 ++ F +K+ DL+ N RAL RL R K LS++++A I+ L +GIDF Sbjct: 271 LIGFMKNSFYQKHGIDLSHNHRALARLEKEALRVKHILSANSEAIASIEELADGIDFRLK 330 Query: 183 ITRARFEELNADLFRSTMEPVEKS 254 ITR+ E L D+ + +EP+ K+ Sbjct: 331 ITRSVLESLCKDMEDAAVEPINKA 354 Score = 41.1 bits (92), Expect = 1e-04 Identities = 23/87 (26%), Positives = 45/87 (51%) Frame = +2 Query: 248 EVFRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNXSINPDEXXXXXXXXXXX 427 + + A + ++I+ I+L GG++RIP +Q L D+ + +++ ++N DE Sbjct: 353 KALKKANLTFSEINSIILFGGASRIPFIQSTLADYVSSDKISKNVNADEASVKGAAFYGA 412 Query: 428 ILHGDKSEEVQDLLLLDVTPLSSVLRL 508 L KS V+ L++ D+ +L L Sbjct: 413 SL--TKSFRVKPLIVQDIINYPYLLSL 437 >SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 29.9 bits (64), Expect = 0.26 Identities = 28/79 (35%), Positives = 37/79 (46%), Gaps = 4/79 (5%) Frame = -3 Query: 475 KQQQILHLLR-LVTVQDSSLDSCTISYG-LVRVNRXV--ELLSIKEILQELLHLGDTSGA 308 K QQ+L LL LV D+ +DS + L ++ V S+ ELL LGD S Sbjct: 932 KHQQLLDLLESLVGNNDNLIDSIKTPHTELQKITDHVLKGTTSLANHTNELLGLGDESLC 991 Query: 307 THQYNIVDLSLIHLGITED 251 + I D SL+ L T D Sbjct: 992 NLETTIEDTSLVKLETTGD 1010 >SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 29.5 bits (63), Expect = 0.34 Identities = 17/43 (39%), Positives = 23/43 (53%), Gaps = 1/43 (2%) Frame = +1 Query: 37 NTKRTSLPTRELLGVCVLHVRGQR-GPCHRPHKRALR*ILSLR 162 N K+ LP E + + + V G GP H+PH R LR + LR Sbjct: 9 NQKKNVLPKPEDIQLIICDVDGTLLGPDHKPHPRNLRALKYLR 51 >SPAC3F10.05c |mug113||DUF1766 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 326 Score = 27.5 bits (58), Expect = 1.4 Identities = 21/95 (22%), Positives = 41/95 (43%), Gaps = 9/95 (9%) Frame = +3 Query: 114 LSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSSVMPRWIRLKS-- 287 +S + + + L G F ++T E ++ + R+ + PV + + PR + S Sbjct: 106 MSEPREVTTIVKDLNNGDSFVLNVT----EPVDPEFLRANIPPVHRKHLPPRLSLVASPG 161 Query: 288 -------TILYWWVAPLVSPRCRSSCKISLMERSS 371 +L W+ PL+S RC+ + L + S Sbjct: 162 TIPTRGVVVLEDWINPLLSERCKLLLQSELCNQDS 196 >SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr 3|||Manual Length = 736 Score = 26.6 bits (56), Expect = 2.4 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = -2 Query: 521 HDTSTVSIPRKGVLHQATADPAPPQTCHRA 432 +DTS ++P LHQ+T P +T RA Sbjct: 294 NDTSFTNVPTSSPLHQSTTANHPEKTPSRA 323 >SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces pombe|chr 1|||Manual Length = 511 Score = 26.2 bits (55), Expect = 3.2 Identities = 9/29 (31%), Positives = 18/29 (62%) Frame = +2 Query: 206 AERRSVQVYHGASGEVFRDAKMDKAQIHD 292 A ++ + + G +F+D K+ K+Q+HD Sbjct: 460 AYKQELNSFQGELSSLFKDLKLVKSQLHD 488 >SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces pombe|chr 1|||Manual Length = 503 Score = 25.8 bits (54), Expect = 4.2 Identities = 19/57 (33%), Positives = 24/57 (42%) Frame = +2 Query: 221 VQVYHGASGEVFRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNXSINPD 391 VQV+ G SG R +D G S RIP + +L FNG L P+ Sbjct: 73 VQVFEGTSGVDVRKTTID--------FTGHSMRIPVSEDMLGRVFNGSGLPIDKGPN 121 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 25.4 bits (53), Expect = 5.5 Identities = 15/46 (32%), Positives = 23/46 (50%) Frame = +3 Query: 3 MVNHFVQEFKRKYKKDLATNKRALRRLRTACERAKRTLSSSTQASI 140 +++ F EFK++ K D + L+ L + C R R SS SI Sbjct: 711 ILSSFSNEFKKRLKSDGTSIAHWLQGLASFCGRVFRRYSSLDCTSI 756 >SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pmc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1292 Score = 25.4 bits (53), Expect = 5.5 Identities = 14/42 (33%), Positives = 26/42 (61%), Gaps = 1/42 (2%) Frame = -3 Query: 313 GATHQYNIVDLSLIHLGITEDFSTGSMVDLNR-SAFSSSKRA 191 G+ + ++D+S+ LG+T S S VD+ + +FSS ++A Sbjct: 634 GSKTETALLDMSVKELGLTNVDSMRSSVDIKQFFSFSSDRKA 675 >SPCC74.04 |||amino acid permease, unknown 15|Schizosaccharomyces pombe|chr 3|||Manual Length = 557 Score = 25.0 bits (52), Expect = 7.3 Identities = 9/24 (37%), Positives = 13/24 (54%) Frame = -1 Query: 147 LSQCSLVWTMTRSSLPSHMQYANA 76 +S C ++WTM+ P HM A Sbjct: 265 MSFCGVIWTMSGYDAPFHMSEETA 288 >SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1029 Score = 24.6 bits (51), Expect = 9.6 Identities = 13/30 (43%), Positives = 16/30 (53%) Frame = +3 Query: 294 LYWWVAPLVSPRCRSSCKISLMERSSTNLL 383 LYW V C+S IS + SST+LL Sbjct: 540 LYWDVQAAKDVDCKSVVDISQLRVSSTSLL 569 >SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1131 Score = 24.6 bits (51), Expect = 9.6 Identities = 14/47 (29%), Positives = 22/47 (46%) Frame = +3 Query: 201 EELNADLFRSTMEPVEKSSVMPRWIRLKSTILYWWVAPLVSPRCRSS 341 EE++ DL V+ + RW LK L ++ PL P +S+ Sbjct: 457 EEMDLDLKSDERFKVDIPTFFERWPFLKDLPLKGYLFPLCEPNLQSA 503 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,230,451 Number of Sequences: 5004 Number of extensions: 41745 Number of successful extensions: 164 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 141 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 161 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 225926624 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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