BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00052 (470 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 100 1e-22 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 100 1e-22 SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 77 2e-15 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 77 2e-15 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 77 2e-15 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 48 5e-07 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 36 0.004 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 27 1.9 SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz... 27 1.9 SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 25 5.8 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 100 bits (240), Expect = 1e-22 Identities = 49/84 (58%), Positives = 57/84 (67%) Frame = +2 Query: 2 GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVXXXXXXXXXXXXXNYSTPXXXXXX 181 GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Y+TP Sbjct: 35 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGKKRKKKTYTTPKKIKHK 94 Query: 182 XXXXXLAVLRFYKVDENGKIHRLR 253 LAVL++YKV+++G + RLR Sbjct: 95 HKKVELAVLKYYKVEDDGSVKRLR 118 Score = 37.9 bits (84), Expect = 8e-04 Identities = 14/23 (60%), Positives = 17/23 (73%) Frame = +1 Query: 274 CGAGVFMAVMEDRHYCGKCHSTM 342 CGA FMA +DR YCG+CH T+ Sbjct: 124 CGASTFMANHKDRLYCGRCHLTL 146 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 100 bits (240), Expect = 1e-22 Identities = 49/84 (58%), Positives = 57/84 (67%) Frame = +2 Query: 2 GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVXXXXXXXXXXXXXNYSTPXXXXXX 181 GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Y+TP Sbjct: 35 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGKKRKKKTYTTPKKIKHK 94 Query: 182 XXXXXLAVLRFYKVDENGKIHRLR 253 LAVL++YKV+++G + RLR Sbjct: 95 HKKVELAVLKYYKVEDDGSVKRLR 118 Score = 37.9 bits (84), Expect = 8e-04 Identities = 14/23 (60%), Positives = 17/23 (73%) Frame = +1 Query: 274 CGAGVFMAVMEDRHYCGKCHSTM 342 CGA FMA +DR YCG+CH T+ Sbjct: 124 CGASTFMANHKDRLYCGRCHLTL 146 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 76.6 bits (180), Expect = 2e-15 Identities = 35/36 (97%), Positives = 36/36 (100%) Frame = +2 Query: 2 GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109 GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 35 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 76.6 bits (180), Expect = 2e-15 Identities = 35/36 (97%), Positives = 36/36 (100%) Frame = +2 Query: 2 GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109 GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 35 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 76.6 bits (180), Expect = 2e-15 Identities = 35/36 (97%), Positives = 36/36 (100%) Frame = +2 Query: 2 GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109 GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 35 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 Score = 76.6 bits (180), Expect = 2e-15 Identities = 35/36 (97%), Positives = 36/36 (100%) Frame = +2 Query: 2 GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109 GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 111 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 146 Score = 76.6 bits (180), Expect = 2e-15 Identities = 35/36 (97%), Positives = 36/36 (100%) Frame = +2 Query: 2 GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109 GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 187 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 222 Score = 76.6 bits (180), Expect = 2e-15 Identities = 35/36 (97%), Positives = 36/36 (100%) Frame = +2 Query: 2 GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109 GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 263 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 298 Score = 76.6 bits (180), Expect = 2e-15 Identities = 35/36 (97%), Positives = 36/36 (100%) Frame = +2 Query: 2 GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109 GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 339 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 374 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 48.4 bits (110), Expect = 5e-07 Identities = 20/36 (55%), Positives = 27/36 (75%) Frame = +2 Query: 2 GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109 GIPP+QQRLI+AGKQ+ D + Y+++ S LHLV Sbjct: 35 GIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLV 70 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 35.5 bits (78), Expect = 0.004 Identities = 16/35 (45%), Positives = 23/35 (65%) Frame = +2 Query: 5 IPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109 I +QRLI+AG+ L+D +L Y IQ ++HLV Sbjct: 39 IEKERQRLIYAGRVLKDEESLKTYKIQDGHSIHLV 73 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 26.6 bits (56), Expect = 1.9 Identities = 10/29 (34%), Positives = 17/29 (58%) Frame = -1 Query: 89 PSGYCSPRGYVHLPAAFQQRSTSVDWEGF 3 PS + PRG V+ P+ ++R + + GF Sbjct: 17 PSSFLKPRGLVYRPSQIRRRVSLLSLSGF 45 >SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 873 Score = 26.6 bits (56), Expect = 1.9 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = -3 Query: 405 SSRERSYRLEGTYLFVVVFEDHRAVTLPAV 316 +S + SY L TYL F++HR + AV Sbjct: 149 NSHQNSYSLNETYLSYDFFDNHRGASSSAV 178 >SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1480 Score = 25.0 bits (52), Expect = 5.8 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +2 Query: 11 PNQQRLIFAGKQLEDGRTLSDYNI 82 PNQ AGK+L+ R L DY+I Sbjct: 476 PNQGDTRKAGKRLKYSRNLDDYHI 499 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,729,234 Number of Sequences: 5004 Number of extensions: 28821 Number of successful extensions: 79 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 72 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 79 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 180421690 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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