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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00052
         (470 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi...   100   1e-22
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch...   100   1e-22
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|...    77   2e-15
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz...    77   2e-15
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M...    77   2e-15
SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro...    48   5e-07
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc...    36   0.004
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom...    27   1.9  
SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz...    27   1.9  
SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch...    25   5.8  

>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
           Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score =  100 bits (240), Expect = 1e-22
 Identities = 49/84 (58%), Positives = 57/84 (67%)
 Frame = +2

Query: 2   GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVXXXXXXXXXXXXXNYSTPXXXXXX 181
           GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV              Y+TP      
Sbjct: 35  GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGKKRKKKTYTTPKKIKHK 94

Query: 182 XXXXXLAVLRFYKVDENGKIHRLR 253
                LAVL++YKV+++G + RLR
Sbjct: 95  HKKVELAVLKYYKVEDDGSVKRLR 118



 Score = 37.9 bits (84), Expect = 8e-04
 Identities = 14/23 (60%), Positives = 17/23 (73%)
 Frame = +1

Query: 274 CGAGVFMAVMEDRHYCGKCHSTM 342
           CGA  FMA  +DR YCG+CH T+
Sbjct: 124 CGASTFMANHKDRLYCGRCHLTL 146


>SPAC589.10c |||ribomal-ubiquitin fusion protein
           Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score =  100 bits (240), Expect = 1e-22
 Identities = 49/84 (58%), Positives = 57/84 (67%)
 Frame = +2

Query: 2   GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVXXXXXXXXXXXXXNYSTPXXXXXX 181
           GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV              Y+TP      
Sbjct: 35  GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGKKRKKKTYTTPKKIKHK 94

Query: 182 XXXXXLAVLRFYKVDENGKIHRLR 253
                LAVL++YKV+++G + RLR
Sbjct: 95  HKKVELAVLKYYKVEDDGSVKRLR 118



 Score = 37.9 bits (84), Expect = 8e-04
 Identities = 14/23 (60%), Positives = 17/23 (73%)
 Frame = +1

Query: 274 CGAGVFMAVMEDRHYCGKCHSTM 342
           CGA  FMA  +DR YCG+CH T+
Sbjct: 124 CGASTFMANHKDRLYCGRCHLTL 146


>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
           Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score = 76.6 bits (180), Expect = 2e-15
 Identities = 35/36 (97%), Positives = 36/36 (100%)
 Frame = +2

Query: 2   GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109
           GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 35  GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70


>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
           Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score = 76.6 bits (180), Expect = 2e-15
 Identities = 35/36 (97%), Positives = 36/36 (100%)
 Frame = +2

Query: 2   GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109
           GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 35  GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70


>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 382

 Score = 76.6 bits (180), Expect = 2e-15
 Identities = 35/36 (97%), Positives = 36/36 (100%)
 Frame = +2

Query: 2   GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109
           GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 35  GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70



 Score = 76.6 bits (180), Expect = 2e-15
 Identities = 35/36 (97%), Positives = 36/36 (100%)
 Frame = +2

Query: 2   GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109
           GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 111 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 146



 Score = 76.6 bits (180), Expect = 2e-15
 Identities = 35/36 (97%), Positives = 36/36 (100%)
 Frame = +2

Query: 2   GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109
           GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 187 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 222



 Score = 76.6 bits (180), Expect = 2e-15
 Identities = 35/36 (97%), Positives = 36/36 (100%)
 Frame = +2

Query: 2   GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109
           GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 263 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 298



 Score = 76.6 bits (180), Expect = 2e-15
 Identities = 35/36 (97%), Positives = 36/36 (100%)
 Frame = +2

Query: 2   GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109
           GIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
Sbjct: 339 GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 374


>SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like
           protein modifier Ned8|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 78

 Score = 48.4 bits (110), Expect = 5e-07
 Identities = 20/36 (55%), Positives = 27/36 (75%)
 Frame = +2

Query: 2   GIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109
           GIPP+QQRLI+AGKQ+ D +    Y+++  S LHLV
Sbjct: 35  GIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLV 70


>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 354

 Score = 35.5 bits (78), Expect = 0.004
 Identities = 16/35 (45%), Positives = 23/35 (65%)
 Frame = +2

Query: 5   IPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 109
           I   +QRLI+AG+ L+D  +L  Y IQ   ++HLV
Sbjct: 39  IEKERQRLIYAGRVLKDEESLKTYKIQDGHSIHLV 73


>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
           Msp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 903

 Score = 26.6 bits (56), Expect = 1.9
 Identities = 10/29 (34%), Positives = 17/29 (58%)
 Frame = -1

Query: 89  PSGYCSPRGYVHLPAAFQQRSTSVDWEGF 3
           PS +  PRG V+ P+  ++R + +   GF
Sbjct: 17  PSSFLKPRGLVYRPSQIRRRVSLLSLSGF 45


>SPAC227.15 |||protein phosphatase regulatory subunit Reg1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 873

 Score = 26.6 bits (56), Expect = 1.9
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = -3

Query: 405 SSRERSYRLEGTYLFVVVFEDHRAVTLPAV 316
           +S + SY L  TYL    F++HR  +  AV
Sbjct: 149 NSHQNSYSLNETYLSYDFFDNHRGASSSAV 178


>SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit
           Rev3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1480

 Score = 25.0 bits (52), Expect = 5.8
 Identities = 12/24 (50%), Positives = 15/24 (62%)
 Frame = +2

Query: 11  PNQQRLIFAGKQLEDGRTLSDYNI 82
           PNQ     AGK+L+  R L DY+I
Sbjct: 476 PNQGDTRKAGKRLKYSRNLDDYHI 499


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,729,234
Number of Sequences: 5004
Number of extensions: 28821
Number of successful extensions: 79
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 72
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 79
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 180421690
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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