BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00043 (753 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 144 1e-35 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 144 1e-35 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 144 1e-35 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 53 4e-08 SPAC3H1.09c |||vacuolar amino acid transporter |Schizosaccharomy... 27 2.9 SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 8.8 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 144 bits (349), Expect = 1e-35 Identities = 62/85 (72%), Positives = 74/85 (87%) Frame = +1 Query: 1 TAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAA 180 TAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA Sbjct: 339 TAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDAC 398 Query: 181 IVNLVPSKPLCVESFQEFPPLGRFA 255 I +VPSKP+CVE+F ++ PLGRFA Sbjct: 399 IAKMVPSKPMCVEAFTDYAPLGRFA 423 Score = 36.3 bits (80), Expect = 0.005 Identities = 17/18 (94%), Positives = 17/18 (94%) Frame = +3 Query: 249 FCVRDMRQTVAVGVIKAV 302 F VRDMRQTVAVGVIKAV Sbjct: 422 FAVRDMRQTVAVGVIKAV 439 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 144 bits (349), Expect = 1e-35 Identities = 62/85 (72%), Positives = 74/85 (87%) Frame = +1 Query: 1 TAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAA 180 TAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA Sbjct: 339 TAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDAC 398 Query: 181 IVNLVPSKPLCVESFQEFPPLGRFA 255 I +VPSKP+CVE+F ++ PLGRFA Sbjct: 399 IAKMVPSKPMCVEAFTDYAPLGRFA 423 Score = 36.3 bits (80), Expect = 0.005 Identities = 17/18 (94%), Positives = 17/18 (94%) Frame = +3 Query: 249 FCVRDMRQTVAVGVIKAV 302 F VRDMRQTVAVGVIKAV Sbjct: 422 FAVRDMRQTVAVGVIKAV 439 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 144 bits (349), Expect = 1e-35 Identities = 62/85 (72%), Positives = 74/85 (87%) Frame = +1 Query: 1 TAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAA 180 TAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA Sbjct: 339 TAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDAC 398 Query: 181 IVNLVPSKPLCVESFQEFPPLGRFA 255 I +VPSKP+CVE+F ++ PLGRFA Sbjct: 399 IAKMVPSKPMCVEAFTDYAPLGRFA 423 Score = 36.3 bits (80), Expect = 0.005 Identities = 17/18 (94%), Positives = 17/18 (94%) Frame = +3 Query: 249 FCVRDMRQTVAVGVIKAV 302 F VRDMRQTVAVGVIKAV Sbjct: 422 FAVRDMRQTVAVGVIKAV 439 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 53.2 bits (122), Expect = 4e-08 Identities = 26/83 (31%), Positives = 45/83 (54%) Frame = +1 Query: 4 AQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAI 183 AQ+ +L P ++ GY+ V+ HTA FA++ K+D +T + ++ P G I Sbjct: 562 AQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLD-KTNRKSKKPPMFATKGMKII 620 Query: 184 VNLVPSKPLCVESFQEFPPLGRF 252 L P+C+E F+++ +GRF Sbjct: 621 AELETQTPVCMERFEDYQYMGRF 643 >SPAC3H1.09c |||vacuolar amino acid transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 656 Score = 27.1 bits (57), Expect = 2.9 Identities = 12/22 (54%), Positives = 13/22 (59%), Gaps = 1/22 (4%) Frame = -2 Query: 542 VSDILCITL-YKYKIMLHYKPC 480 V + CI L Y Y MLHYK C Sbjct: 605 VGSVCCIPLIYMYPPMLHYKAC 626 >SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 268 Score = 25.4 bits (53), Expect = 8.8 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = -2 Query: 182 MAASPDLMDFGLTSVDLPVRRSTFS 108 ++ +PDL D L+SVD P++ +T S Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,882,642 Number of Sequences: 5004 Number of extensions: 56383 Number of successful extensions: 152 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 141 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 151 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 359287726 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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