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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00043
         (753 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   144   1e-35
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   144   1e-35
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   144   1e-35
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    53   4e-08
SPAC3H1.09c |||vacuolar amino acid transporter |Schizosaccharomy...    27   2.9  
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos...    25   8.8  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  144 bits (349), Expect = 1e-35
 Identities = 62/85 (72%), Positives = 74/85 (87%)
 Frame = +1

Query: 1   TAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAA 180
           TAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA 
Sbjct: 339 TAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDAC 398

Query: 181 IVNLVPSKPLCVESFQEFPPLGRFA 255
           I  +VPSKP+CVE+F ++ PLGRFA
Sbjct: 399 IAKMVPSKPMCVEAFTDYAPLGRFA 423



 Score = 36.3 bits (80), Expect = 0.005
 Identities = 17/18 (94%), Positives = 17/18 (94%)
 Frame = +3

Query: 249 FCVRDMRQTVAVGVIKAV 302
           F VRDMRQTVAVGVIKAV
Sbjct: 422 FAVRDMRQTVAVGVIKAV 439


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  144 bits (349), Expect = 1e-35
 Identities = 62/85 (72%), Positives = 74/85 (87%)
 Frame = +1

Query: 1   TAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAA 180
           TAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA 
Sbjct: 339 TAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDAC 398

Query: 181 IVNLVPSKPLCVESFQEFPPLGRFA 255
           I  +VPSKP+CVE+F ++ PLGRFA
Sbjct: 399 IAKMVPSKPMCVEAFTDYAPLGRFA 423



 Score = 36.3 bits (80), Expect = 0.005
 Identities = 17/18 (94%), Positives = 17/18 (94%)
 Frame = +3

Query: 249 FCVRDMRQTVAVGVIKAV 302
           F VRDMRQTVAVGVIKAV
Sbjct: 422 FAVRDMRQTVAVGVIKAV 439


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  144 bits (349), Expect = 1e-35
 Identities = 62/85 (72%), Positives = 74/85 (87%)
 Frame = +1

Query: 1   TAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAA 180
           TAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA 
Sbjct: 339 TAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDAC 398

Query: 181 IVNLVPSKPLCVESFQEFPPLGRFA 255
           I  +VPSKP+CVE+F ++ PLGRFA
Sbjct: 399 IAKMVPSKPMCVEAFTDYAPLGRFA 423



 Score = 36.3 bits (80), Expect = 0.005
 Identities = 17/18 (94%), Positives = 17/18 (94%)
 Frame = +3

Query: 249 FCVRDMRQTVAVGVIKAV 302
           F VRDMRQTVAVGVIKAV
Sbjct: 422 FAVRDMRQTVAVGVIKAV 439


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 53.2 bits (122), Expect = 4e-08
 Identities = 26/83 (31%), Positives = 45/83 (54%)
 Frame = +1

Query: 4   AQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAI 183
           AQ+ +L  P  ++ GY+ V+  HTA     FA++  K+D +T + ++  P     G   I
Sbjct: 562 AQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLD-KTNRKSKKPPMFATKGMKII 620

Query: 184 VNLVPSKPLCVESFQEFPPLGRF 252
             L    P+C+E F+++  +GRF
Sbjct: 621 AELETQTPVCMERFEDYQYMGRF 643


>SPAC3H1.09c |||vacuolar amino acid transporter |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 656

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 12/22 (54%), Positives = 13/22 (59%), Gaps = 1/22 (4%)
 Frame = -2

Query: 542 VSDILCITL-YKYKIMLHYKPC 480
           V  + CI L Y Y  MLHYK C
Sbjct: 605 VGSVCCIPLIYMYPPMLHYKAC 626


>SPAC631.01c |acp2||F-actin capping protein beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 268

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = -2

Query: 182 MAASPDLMDFGLTSVDLPVRRSTFS 108
           ++ +PDL D  L+SVD P++ +T S
Sbjct: 27  LSVAPDLADVLLSSVDQPLKVNTCS 51


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,882,642
Number of Sequences: 5004
Number of extensions: 56383
Number of successful extensions: 152
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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