BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00034 (681 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces pombe... 64 1e-11 SPAPB17E12.11 |||N-oligosaccharyltransferase gamma subunit |Schi... 29 0.47 SPBC106.20 |exo70|SPBC582.02|exocyst complex subunit Exo70 |Schi... 28 1.1 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 27 1.9 SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosacchar... 27 2.5 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.5 SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c... 25 7.7 SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 25 7.7 >SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 425 Score = 64.5 bits (150), Expect = 1e-11 Identities = 37/87 (42%), Positives = 55/87 (63%), Gaps = 5/87 (5%) Frame = +3 Query: 264 AYENARFLCDRYY--LASPELEVLQDGVSSLRPMPIVYVPSHLYHMLFELFKNAMRAVME 437 A ENA+++C Y +PE++++ D SL M YV SHL H +FE+ KN++RA +E Sbjct: 253 AAENAKYICRLAYGLFEAPEIQIICD--PSLEMM---YVESHLNHAVFEILKNSLRATVE 307 Query: 438 NHGTAP---PPIQVNLVNGKEDISVKI 509 HG PPI+V + G+EDI++KI Sbjct: 308 FHGVDSDFFPPIKVIVAKGQEDITIKI 334 Score = 46.8 bits (106), Expect = 3e-06 Identities = 22/37 (59%), Positives = 26/37 (70%) Frame = +1 Query: 52 RRDPGTENSIQYFLDRFYMSRISIRMLINQHTLLFGE 162 R+ +NSIQ FLDRFYMSRI IRML+ Q+ L E Sbjct: 194 RKTNQIDNSIQIFLDRFYMSRIGIRMLLGQYIALVSE 230 Score = 29.5 bits (63), Expect = 0.47 Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 7/36 (19%) Frame = +1 Query: 553 FKYMYSTAPQPSKSDSH-------TVPLAGYGYGLP 639 + YM++TA D H T P+AG+G+GLP Sbjct: 350 WSYMFTTASPTLTDDPHDIVSANSTTPMAGFGFGLP 385 Score = 25.4 bits (53), Expect = 7.7 Identities = 10/16 (62%), Positives = 12/16 (75%) Frame = +2 Query: 632 GCLISRLYARYFHGDL 679 G ++RLY RYF GDL Sbjct: 383 GLPLARLYTRYFGGDL 398 >SPAPB17E12.11 |||N-oligosaccharyltransferase gamma subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 309 Score = 29.5 bits (63), Expect = 0.47 Identities = 16/55 (29%), Positives = 28/55 (50%) Frame = +3 Query: 474 LVNGKEDISVKILIAAAASRAVSELLFQVHVQHGAAALQVRLAHGAASGLRIRAA 638 +VNGK+D + L +A +S ++ + + A A +L +G SG+R A Sbjct: 43 IVNGKQDFTTVALFSADSSTMNCDVCRLIEPEFKALANSYKLKYGLDSGIRFTYA 97 >SPBC106.20 |exo70|SPBC582.02|exocyst complex subunit Exo70 |Schizosaccharomyces pombe|chr 2|||Manual Length = 615 Score = 28.3 bits (60), Expect = 1.1 Identities = 16/48 (33%), Positives = 25/48 (52%) Frame = +3 Query: 468 VNLVNGKEDISVKILIAAAASRAVSELLFQVHVQHGAAALQVRLAHGA 611 VN V GKED+S+ ++ + AV+ L + Q A AL ++ A Sbjct: 239 VNFVTGKEDVSINLVALSRLLPAVASELLLLFDQVTAKALYPKIVKPA 286 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/27 (48%), Positives = 19/27 (70%) Frame = +1 Query: 562 MYSTAPQPSKSDSHTVPLAGYGYGLPN 642 MYS++ Q S+S S+T AG G+G P+ Sbjct: 870 MYSSSRQSSRSGSNTHSHAGPGFGGPS 896 >SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosaccharomyces pombe|chr 1|||Manual Length = 1033 Score = 27.1 bits (57), Expect = 2.5 Identities = 17/41 (41%), Positives = 22/41 (53%) Frame = +1 Query: 175 KQASVNGIGNGGRHIGSIDPACDVVAVLGTLTRTQGSSATD 297 KQASV IG+GG +G I A + ++G Q S A D Sbjct: 761 KQASVCCIGDGGNDVGMIQVANVGIGIVGK-EGQQASLAAD 800 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 27.1 bits (57), Expect = 2.5 Identities = 21/83 (25%), Positives = 30/83 (36%) Frame = +1 Query: 256 LGTLTRTQGSSATDTT*PHQSWRYCRTECRVCARCP*CMCPRTSTTCCLNYSRTP*EPSW 435 L T T +S T+ P S T C P P TST T + Sbjct: 445 LATTNCTTSTSVPYTSTPVTSTPLTTTNCTTSTSIPYTSTPVTSTPLTTTNCTTSTSVPY 504 Query: 436 RTTALPRRRYKLTSSTGKRTYPL 504 +T + Y ++SST + P+ Sbjct: 505 TSTPVTSSNYTISSSTPVTSTPV 527 >SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 25.4 bits (53), Expect = 7.7 Identities = 16/60 (26%), Positives = 27/60 (45%), Gaps = 3/60 (5%) Frame = +1 Query: 448 LPRRRYKLTSSTGKRTYPLRF*S---RRRHPAQCRSFCFKYMYSTAPQPSKSDSHTVPLA 618 LP+ RY LT + YP R S ++ RS+ + T QP+ + + P++ Sbjct: 57 LPKSRYALTKRSSSSEYPRRSASTSAKKNVIPITRSYSTTFFSKTNDQPTVTGNQDKPIS 116 >SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1953 Score = 25.4 bits (53), Expect = 7.7 Identities = 15/55 (27%), Positives = 29/55 (52%), Gaps = 2/55 (3%) Frame = +3 Query: 282 FLCDRY--YLASPELEVLQDGVSSLRPMPIVYVPSHLYHMLFELFKNAMRAVMEN 440 F DR+ YLA + +++ VS + + ++YVP+ +F ++K V +N Sbjct: 428 FALDRFGDYLADQVVAPIRESVSQVLGVALIYVPN---DSVFSMYKVLHSLVFQN 479 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,826,518 Number of Sequences: 5004 Number of extensions: 57053 Number of successful extensions: 188 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 169 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 186 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 313902888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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