BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00034 (681 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g06483.1 68416.m00751 pyruvate dehydrogenase (lipoamide) kina... 60 1e-09 At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putativ... 28 5.0 >At3g06483.1 68416.m00751 pyruvate dehydrogenase (lipoamide) kinase (PDHK) nearly identical to pyruvate dehydrogenase kinase [Arabidopsis thaliana] GI:3641834 Length = 369 Score = 60.1 bits (139), Expect = 1e-09 Identities = 31/86 (36%), Positives = 53/86 (61%), Gaps = 3/86 (3%) Frame = +3 Query: 261 DAYENARFLCDRYYLASPELEVLQDGVSSLRPMPIVYVPSHLYHMLFELFKNAMRAVME- 437 +A E+AR +C R Y ++PE+ + D YVP+HL+ M++EL KN++RAV E Sbjct: 199 NASEDARSICFREYGSAPEINIYGDP-----SFTFPYVPTHLHLMMYELVKNSLRAVQER 253 Query: 438 --NHGTAPPPIQVNLVNGKEDISVKI 509 + PPI++ + +G ED+++K+ Sbjct: 254 FVDSDRVAPPIRIIVADGIEDVTIKV 279 Score = 35.9 bits (79), Expect = 0.025 Identities = 16/26 (61%), Positives = 19/26 (73%) Frame = +1 Query: 64 GTENSIQYFLDRFYMSRISIRMLINQ 141 G + I FLDRFY+SRI IRMLI + Sbjct: 142 GNLDEIHQFLDRFYLSRIGIRMLIGK 167 Score = 32.7 bits (71), Expect = 0.23 Identities = 17/35 (48%), Positives = 20/35 (57%), Gaps = 6/35 (17%) Frame = +1 Query: 553 FKYMYSTAPQPSKSDSHT------VPLAGYGYGLP 639 F Y+YSTA P + D V +AGYGYGLP Sbjct: 295 FTYLYSTARNPLEEDVDLGIADVPVTMAGYGYGLP 329 Score = 29.1 bits (62), Expect = 2.8 Identities = 13/16 (81%), Positives = 13/16 (81%) Frame = +2 Query: 632 GCLISRLYARYFHGDL 679 G ISRLYARYF GDL Sbjct: 327 GLPISRLYARYFGGDL 342 >At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putative (UBP23) identical to GI:11993486 Length = 859 Score = 28.3 bits (60), Expect = 5.0 Identities = 18/53 (33%), Positives = 29/53 (54%) Frame = +1 Query: 196 IGNGGRHIGSIDPACDVVAVLGTLTRTQGSSATDTT*PHQSWRYCRTECRVCA 354 IG G +++G+ C + +VL LT T+ +AT T HQ + + C +CA Sbjct: 105 IGAGLQNLGN---TCFLNSVLQCLTYTEPLAATLQTAAHQKYCHVAGFCALCA 154 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,724,790 Number of Sequences: 28952 Number of extensions: 338604 Number of successful extensions: 999 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 969 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 997 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1438152744 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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