BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00026 (700 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC23G7.05 |sui1|psu1|translation initiation factor eIF1|Schizo... 62 1e-10 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 29 0.85 SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3 |Schizos... 28 1.1 SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1 |Schiz... 27 3.4 SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr 1... 25 7.9 SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 25 7.9 >SPBC23G7.05 |sui1|psu1|translation initiation factor eIF1|Schizosaccharomyces pombe|chr 2|||Manual Length = 109 Score = 61.7 bits (143), Expect = 1e-10 Identities = 31/47 (65%), Positives = 36/47 (76%), Gaps = 1/47 (2%) Frame = +1 Query: 112 SIQNLNTFDPFADAIKSSEDDVQD-GLVHVRIQQRNGRKTLTTVQGL 249 +IQN NT DPFAD + +DD Q +H+RIQQRNGRKTLTTVQGL Sbjct: 3 AIQNFNTVDPFAD---TGDDDAQPLNNIHIRIQQRNGRKTLTTVQGL 46 Score = 47.2 bits (107), Expect = 2e-06 Identities = 19/43 (44%), Positives = 32/43 (74%) Frame = +2 Query: 308 GTVVEHPEYGEVLQLQGDQRENICQWLTKSGLVKPEQLKVHGF 436 GT+V+ + GEV+QLQGDQR + ++LT+ ++ + +K+HGF Sbjct: 67 GTIVKDDDLGEVIQLQGDQRIKVMEFLTQQLGLQKKNIKIHGF 109 Score = 29.5 bits (63), Expect = 0.48 Identities = 11/17 (64%), Positives = 15/17 (88%) Frame = +3 Query: 255 EYDLKKIVRACKKEFAC 305 E+D K+I+RA KK+FAC Sbjct: 49 EFDQKRILRALKKDFAC 65 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 28.7 bits (61), Expect = 0.85 Identities = 14/48 (29%), Positives = 25/48 (52%) Frame = -1 Query: 310 TLHANSFLHARTIFFRSYSRKGLAP*SASYARFVAGYGRGLNHLERRP 167 T ANSF + + F+ + P + S +F+ G+G+G+ L +P Sbjct: 2795 TAGANSFYDSMSSGFKGLKKPFTDPKNNSAGKFLKGFGKGMLGLATKP 2842 >SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 790 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/32 (37%), Positives = 21/32 (65%) Frame = -2 Query: 171 VLRAFDSIGEWVECVEILDGHTIERRVSLFQR 76 +LR S+ W+ ++ +G T+E+RV LF+R Sbjct: 25 LLRDPYSLKSWLRYIKTHEGSTLEKRVLLFER 56 >SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 451 Score = 26.6 bits (56), Expect = 3.4 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = -3 Query: 290 LACPHDLLQVIFEERPCTVVSV 225 ++CP LL VIFE RP ++++ Sbjct: 114 VSCPVGLLLVIFEARPEVIINI 135 >SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 455 Score = 25.4 bits (53), Expect = 7.9 Identities = 17/48 (35%), Positives = 26/48 (54%), Gaps = 6/48 (12%) Frame = +2 Query: 275 RAGMQEGVRVQGTVVEH-PEYGEVL-----QLQGDQRENICQWLTKSG 400 RAG + V V G++VEH P + ++L +L GD + I + K G Sbjct: 388 RAGKKSDVGVDGSLVEHYPHFVDMLREALRELIGDNEKLISIGIAKDG 435 >SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 25.4 bits (53), Expect = 7.9 Identities = 17/62 (27%), Positives = 25/62 (40%), Gaps = 1/62 (1%) Frame = +1 Query: 73 VSLKQRDPTFNRMSIQNLNTFDPFAD-AIKSSEDDVQDGLVHVRIQQRNGRKTLTTVQGL 249 V + P S++ L D AI D + + H R+Q +N R T QG+ Sbjct: 50 VEKRLTSPLMESHSVEKLFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQGI 109 Query: 250 SS 255 S Sbjct: 110 ES 111 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,730,924 Number of Sequences: 5004 Number of extensions: 54195 Number of successful extensions: 164 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 157 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 163 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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