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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00021
         (562 letters)

Database: fruitfly 
           53,049 sequences; 24,988,368 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY058721-1|AAL13950.1|  260|Drosophila melanogaster LD46306p pro...   167   9e-42
AE014298-1969|AAF48326.1|  260|Drosophila melanogaster CG10997-P...   167   9e-42
AY071648-1|AAL49270.1|  215|Drosophila melanogaster RE70889p pro...    32   0.61 
AE014297-1491|AAF54789.1|  215|Drosophila melanogaster CG11512-P...    32   0.61 
U25188-1|AAA86888.1|  277|Drosophila melanogaster initiation fac...    31   1.1  
U02461-1|AAA81888.1|  276|Drosophila melanogaster dTFIIF30 protein.    31   1.1  
AY071514-1|AAL49136.1|  277|Drosophila melanogaster RE56801p pro...    31   1.1  
AE014297-1190|AAF54548.1|  277|Drosophila melanogaster CG6538-PA...    31   1.1  
AE014297-1492|AAF54790.3|  216|Drosophila melanogaster CG12242-P...    29   3.3  
BT023794-1|AAZ41803.1|  409|Drosophila melanogaster GH05001p pro...    29   5.7  
AY058474-1|AAL13703.1|  434|Drosophila melanogaster GH27792p pro...    29   5.7  
AL009196-5|CAA15710.1|  434|Drosophila melanogaster EG:25E8.4 pr...    29   5.7  
AE014298-2746|AAF48865.1|  832|Drosophila melanogaster CG6606-PA...    29   5.7  
AE014298-381|AAF45770.1|  434|Drosophila melanogaster CG2865-PA ...    29   5.7  
AE014296-2144|AAF49916.1|  733|Drosophila melanogaster CG10663-P...    29   5.7  

>AY058721-1|AAL13950.1|  260|Drosophila melanogaster LD46306p
           protein.
          Length = 260

 Score =  167 bits (406), Expect = 9e-42
 Identities = 78/85 (91%), Positives = 84/85 (98%)
 Frame = +3

Query: 3   ELKTISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMKSVPGGHNL 182
           ELKTISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMK++PGG+NL
Sbjct: 55  ELKTISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMKNIPGGYNL 114

Query: 183 FVQDKEVASLIENLYSKLKLVLVRK 257
           FVQDKEVA+LIENLY KLKL+LV+K
Sbjct: 115 FVQDKEVATLIENLYVKLKLMLVKK 139



 Score =  147 bits (356), Expect = 1e-35
 Identities = 64/85 (75%), Positives = 72/85 (84%)
 Frame = +2

Query: 254 QDEQKSAALRAHLGRIDGLLERRETRFLTGDTMCCFDCELMPKVQHIRVGGKYFVDFQIP 433
           +DE K+ AL +HL +I+  L  R TRFLTGDTMCCFDCELMP++QHIRV GKYFVDF+IP
Sbjct: 139 KDEAKNNALLSHLRKINDHLSARNTRFLTGDTMCCFDCELMPRLQHIRVAGKYFVDFEIP 198

Query: 434 TTFRALWRYMYHMYQLDAFTQSCPA 508
           T   ALWRYMYHMYQLDAFTQSCPA
Sbjct: 199 THLTALWRYMYHMYQLDAFTQSCPA 223



 Score = 36.3 bits (80), Expect = 0.028
 Identities = 15/17 (88%), Positives = 17/17 (100%)
 Frame = +1

Query: 511 QDIINHYKLQQALKMKE 561
           QDIINHYKLQQ+LKMK+
Sbjct: 225 QDIINHYKLQQSLKMKK 241


>AE014298-1969|AAF48326.1|  260|Drosophila melanogaster CG10997-PA
           protein.
          Length = 260

 Score =  167 bits (406), Expect = 9e-42
 Identities = 78/85 (91%), Positives = 84/85 (98%)
 Frame = +3

Query: 3   ELKTISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMKSVPGGHNL 182
           ELKTISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMK++PGG+NL
Sbjct: 55  ELKTISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMKNIPGGYNL 114

Query: 183 FVQDKEVASLIENLYSKLKLVLVRK 257
           FVQDKEVA+LIENLY KLKL+LV+K
Sbjct: 115 FVQDKEVATLIENLYVKLKLMLVKK 139



 Score =  147 bits (356), Expect = 1e-35
 Identities = 64/85 (75%), Positives = 72/85 (84%)
 Frame = +2

Query: 254 QDEQKSAALRAHLGRIDGLLERRETRFLTGDTMCCFDCELMPKVQHIRVGGKYFVDFQIP 433
           +DE K+ AL +HL +I+  L  R TRFLTGDTMCCFDCELMP++QHIRV GKYFVDF+IP
Sbjct: 139 KDEAKNNALLSHLRKINDHLSARNTRFLTGDTMCCFDCELMPRLQHIRVAGKYFVDFEIP 198

Query: 434 TTFRALWRYMYHMYQLDAFTQSCPA 508
           T   ALWRYMYHMYQLDAFTQSCPA
Sbjct: 199 THLTALWRYMYHMYQLDAFTQSCPA 223



 Score = 36.3 bits (80), Expect = 0.028
 Identities = 15/17 (88%), Positives = 17/17 (100%)
 Frame = +1

Query: 511 QDIINHYKLQQALKMKE 561
           QDIINHYKLQQ+LKMK+
Sbjct: 225 QDIINHYKLQQSLKMKK 241


>AY071648-1|AAL49270.1|  215|Drosophila melanogaster RE70889p
           protein.
          Length = 215

 Score = 31.9 bits (69), Expect = 0.61
 Identities = 24/76 (31%), Positives = 39/76 (51%), Gaps = 1/76 (1%)
 Frame = +3

Query: 3   ELKTISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMKSVPGGHNL 182
           EL    L++T  +  KP    + N + T P  L+DNG AI E+  I  ++++      +L
Sbjct: 25  ELNKKQLRITEGEHLKPE-FLKLNPQHTIPT-LVDNGFAIWESRAIAVYLVEKYGKDDSL 82

Query: 183 FVQDKEVASLI-ENLY 227
           F  D +  +LI + LY
Sbjct: 83  FPNDPQKRALINQRLY 98


>AE014297-1491|AAF54789.1|  215|Drosophila melanogaster CG11512-PA
           protein.
          Length = 215

 Score = 31.9 bits (69), Expect = 0.61
 Identities = 24/76 (31%), Positives = 39/76 (51%), Gaps = 1/76 (1%)
 Frame = +3

Query: 3   ELKTISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMKSVPGGHNL 182
           EL    L++T  +  KP    + N + T P  L+DNG AI E+  I  ++++      +L
Sbjct: 25  ELNKKQLRITEGEHLKPE-FLKLNPQHTIPT-LVDNGFAIWESRAIAVYLVEKYGKDDSL 82

Query: 183 FVQDKEVASLI-ENLY 227
           F  D +  +LI + LY
Sbjct: 83  FPNDPQKRALINQRLY 98


>U25188-1|AAA86888.1|  277|Drosophila melanogaster initiation factor
           TFIIF smallsubunit protein.
          Length = 277

 Score = 31.1 bits (67), Expect = 1.1
 Identities = 16/42 (38%), Positives = 22/42 (52%)
 Frame = +3

Query: 186 VQDKEVASLIENLYSKLKLVLVRKMNRNQRRYELISDASMDF 311
           VQ  E   + +N Y KLKL  +RK +  QRR + I     +F
Sbjct: 129 VQKLECRPIADNCYMKLKLESIRKASEPQRRVQPIDKIVQNF 170


>U02461-1|AAA81888.1|  276|Drosophila melanogaster dTFIIF30 protein.
          Length = 276

 Score = 31.1 bits (67), Expect = 1.1
 Identities = 16/42 (38%), Positives = 22/42 (52%)
 Frame = +3

Query: 186 VQDKEVASLIENLYSKLKLVLVRKMNRNQRRYELISDASMDF 311
           VQ  E   + +N Y KLKL  +RK +  QRR + I     +F
Sbjct: 128 VQKLECRPIADNCYMKLKLESIRKASEPQRRVQPIDKIVQNF 169


>AY071514-1|AAL49136.1|  277|Drosophila melanogaster RE56801p
           protein.
          Length = 277

 Score = 31.1 bits (67), Expect = 1.1
 Identities = 16/42 (38%), Positives = 22/42 (52%)
 Frame = +3

Query: 186 VQDKEVASLIENLYSKLKLVLVRKMNRNQRRYELISDASMDF 311
           VQ  E   + +N Y KLKL  +RK +  QRR + I     +F
Sbjct: 129 VQKLECRPIADNCYMKLKLESIRKASEPQRRVQPIDKIVQNF 170


>AE014297-1190|AAF54548.1|  277|Drosophila melanogaster CG6538-PA
           protein.
          Length = 277

 Score = 31.1 bits (67), Expect = 1.1
 Identities = 16/42 (38%), Positives = 22/42 (52%)
 Frame = +3

Query: 186 VQDKEVASLIENLYSKLKLVLVRKMNRNQRRYELISDASMDF 311
           VQ  E   + +N Y KLKL  +RK +  QRR + I     +F
Sbjct: 129 VQKLECRPIADNCYMKLKLESIRKASEPQRRVQPIDKIVQNF 170


>AE014297-1492|AAF54790.3|  216|Drosophila melanogaster CG12242-PA
           protein.
          Length = 216

 Score = 29.5 bits (63), Expect = 3.3
 Identities = 18/69 (26%), Positives = 35/69 (50%), Gaps = 2/69 (2%)
 Frame = +3

Query: 27  VTTVDMQKPPPDFRTNFEATHP-PILIDNGLAILENEKIERHIMKSVPGGHNLFVQDKEV 203
           + T++  +  P+F       H  P L+DNG +I E+  I  ++++       LF +D + 
Sbjct: 31  LNTLEKDQLKPEF-VKLNPQHTIPTLVDNGFSIWESRAIAVYLVEKYGKDDTLFPKDPKK 89

Query: 204 ASLI-ENLY 227
            +L+ + LY
Sbjct: 90  QALVNQRLY 98


>BT023794-1|AAZ41803.1|  409|Drosophila melanogaster GH05001p
           protein.
          Length = 409

 Score = 28.7 bits (61), Expect = 5.7
 Identities = 13/34 (38%), Positives = 20/34 (58%)
 Frame = +3

Query: 18  SLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLA 119
           SL +  + +Q PPP    NF   HP  L++NG++
Sbjct: 263 SLNIQRIGLQ-PPPVGTPNFTPRHPSPLLNNGVS 295


>AY058474-1|AAL13703.1|  434|Drosophila melanogaster GH27792p
           protein.
          Length = 434

 Score = 28.7 bits (61), Expect = 5.7
 Identities = 16/46 (34%), Positives = 27/46 (58%)
 Frame = +3

Query: 162 VPGGHNLFVQDKEVASLIENLYSKLKLVLVRKMNRNQRRYELISDA 299
           +PGG  + + D    +   +L+  L+LV    MN+NQ+ +E+I DA
Sbjct: 350 MPGGKRMKLNDHHHLNHHHHLHHHLELVDF-DMNQNQKDFEVIMDA 394


>AL009196-5|CAA15710.1|  434|Drosophila melanogaster EG:25E8.4
           protein.
          Length = 434

 Score = 28.7 bits (61), Expect = 5.7
 Identities = 16/46 (34%), Positives = 27/46 (58%)
 Frame = +3

Query: 162 VPGGHNLFVQDKEVASLIENLYSKLKLVLVRKMNRNQRRYELISDA 299
           +PGG  + + D    +   +L+  L+LV    MN+NQ+ +E+I DA
Sbjct: 350 MPGGKRMKLNDHHHLNHHHHLHHHLELVDF-DMNQNQKDFEVIMDA 394


>AE014298-2746|AAF48865.1|  832|Drosophila melanogaster CG6606-PA
           protein.
          Length = 832

 Score = 28.7 bits (61), Expect = 5.7
 Identities = 13/34 (38%), Positives = 20/34 (58%)
 Frame = +3

Query: 18  SLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLA 119
           SL +  + +Q PPP    NF   HP  L++NG++
Sbjct: 263 SLNIQRIGLQ-PPPVGTPNFTPRHPSPLLNNGVS 295


>AE014298-381|AAF45770.1|  434|Drosophila melanogaster CG2865-PA
           protein.
          Length = 434

 Score = 28.7 bits (61), Expect = 5.7
 Identities = 16/46 (34%), Positives = 27/46 (58%)
 Frame = +3

Query: 162 VPGGHNLFVQDKEVASLIENLYSKLKLVLVRKMNRNQRRYELISDA 299
           +PGG  + + D    +   +L+  L+LV    MN+NQ+ +E+I DA
Sbjct: 350 MPGGKRMKLNDHHHLNHHHHLHHHLELVDF-DMNQNQKDFEVIMDA 394


>AE014296-2144|AAF49916.1|  733|Drosophila melanogaster CG10663-PA
           protein.
          Length = 733

 Score = 28.7 bits (61), Expect = 5.7
 Identities = 9/17 (52%), Positives = 14/17 (82%)
 Frame = +3

Query: 48  KPPPDFRTNFEATHPPI 98
           KPPP++ + + +THPPI
Sbjct: 153 KPPPNYASLYPSTHPPI 169


  Database: fruitfly
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 24,988,368
  Number of sequences in database:  53,049
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,911,284
Number of Sequences: 53049
Number of extensions: 502238
Number of successful extensions: 1588
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1515
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1587
length of database: 24,988,368
effective HSP length: 81
effective length of database: 20,691,399
effective search space used: 2172596895
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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