BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00010 (717 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC119.04 |mei3||meiosis inducing protein Mei3 |Schizosaccharom... 28 1.5 SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces pombe... 27 2.7 SPCC4B3.08 |||C-terminal domain kinase I |Schizosaccharomyces po... 27 2.7 SPBC1778.09 |||GTPase activating protein |Schizosaccharomyces po... 27 2.7 SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyce... 27 3.5 SPAC29E6.02 |prp3|SPAC30.06|U4/U6 x U5 tri-snRNP complex subunit... 27 3.5 SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 26 4.7 SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyc... 26 4.7 SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 26 6.2 SPBC4F6.10 |vps901|vps9a|guanyl-nucleotide exchange factor Vps90... 26 6.2 SPCC24B10.11c |||THO complex subunit 7 |Schizosaccharomyces pomb... 25 8.2 >SPBC119.04 |mei3||meiosis inducing protein Mei3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 148 Score = 27.9 bits (59), Expect = 1.5 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +2 Query: 185 KTVEKFAATLSDDVKKDPKKIEAGSKSSVKVQRTKKI 295 KTV+ T K + +AGS SV ++RTK++ Sbjct: 46 KTVDHAGTTFISSTSKRGRSTKAGSVHSVPMKRTKRV 82 >SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 652 Score = 27.1 bits (57), Expect = 2.7 Identities = 13/41 (31%), Positives = 23/41 (56%) Frame = +2 Query: 173 EVCVKTVEKFAATLSDDVKKDPKKIEAGSKSSVKVQRTKKI 295 E+ K+ E ++ + K+ P+K + KSS KVQ+ K+ Sbjct: 14 ELAGKSYEALLYLMNKNKKRKPEKKDDKEKSSKKVQKKNKV 54 >SPCC4B3.08 |||C-terminal domain kinase I |Schizosaccharomyces pombe|chr 3|||Manual Length = 218 Score = 27.1 bits (57), Expect = 2.7 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = +3 Query: 498 EEDSNDWDEVCDGCIEKTDFIKRIEELKPKY 590 E+ S D G I K D +KRIEE + ++ Sbjct: 138 EQASKSGDTSTSGAISKNDILKRIEEDRERH 168 >SPBC1778.09 |||GTPase activating protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 414 Score = 27.1 bits (57), Expect = 2.7 Identities = 13/30 (43%), Positives = 20/30 (66%) Frame = +1 Query: 373 SMPADKICEKLKKKDAQICDLRFDKQIDLN 462 S+P K+ +K + + QICD +D QIDL+ Sbjct: 175 SLPDKKLIDKYYQLNEQICD--YDVQIDLD 202 >SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 26.6 bits (56), Expect = 3.5 Identities = 20/89 (22%), Positives = 42/89 (47%), Gaps = 3/89 (3%) Frame = +2 Query: 41 YIEVSKFITHEFKYSSKMFKFSLFHLLFLATAVQVVLSLREGDCEVCVK---TVEKFAAT 211 Y+++SK I H +K + +F+ +F A Q + + ++ ++ T+ Sbjct: 70 YMKLSKVIVHPYK-DANVFEEHGIQDIFAALRDQTAAIASDSE-QISIQLPGTIINILEL 127 Query: 212 LSDDVKKDPKKIEAGSKSSVKVQRTKKID 298 L +D+++ KKI A VKV ++ + Sbjct: 128 LREDLREHCKKIAAEGTKGVKVVEKQRAE 156 >SPAC29E6.02 |prp3|SPAC30.06|U4/U6 x U5 tri-snRNP complex subunit Prp3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 542 Score = 26.6 bits (56), Expect = 3.5 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = +2 Query: 212 LSDDVKKDPKKIEAGSKSSVKVQRTK 289 L DD KDP KIEA + V+ +R + Sbjct: 342 LGDDAIKDPTKIEAEVRKQVEERRLR 367 >SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces pombe|chr 2|||Manual Length = 1485 Score = 26.2 bits (55), Expect = 4.7 Identities = 13/28 (46%), Positives = 18/28 (64%) Frame = +1 Query: 352 LSKPLSWSMPADKICEKLKKKDAQICDL 435 LS PL WS+ ++ E LKKKD + +L Sbjct: 1153 LSMPL-WSLTYERYVELLKKKDEVMAEL 1179 >SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 490 Score = 26.2 bits (55), Expect = 4.7 Identities = 16/60 (26%), Positives = 25/60 (41%), Gaps = 3/60 (5%) Frame = +1 Query: 262 KFCKGSKNKENRFCYYLGGLEESATGILGELS---KPLSWSMPADKICEKLKKKDAQICD 432 + S + +NR+ YY L E + ILG + +P MP + E +K D Sbjct: 90 RISSNSPSAKNRYIYYPDRLNEIPSSILGSIKSIMQPALRPMPLAMMLEPFRKSKRDSTD 149 >SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|Schizosaccharomyces pombe|chr 2|||Manual Length = 1398 Score = 25.8 bits (54), Expect = 6.2 Identities = 7/10 (70%), Positives = 9/10 (90%) Frame = +3 Query: 510 NDWDEVCDGC 539 +DWD+ CDGC Sbjct: 340 DDWDDSCDGC 349 >SPBC4F6.10 |vps901|vps9a|guanyl-nucleotide exchange factor Vps901 |Schizosaccharomyces pombe|chr 2|||Manual Length = 537 Score = 25.8 bits (54), Expect = 6.2 Identities = 22/90 (24%), Positives = 41/90 (45%), Gaps = 1/90 (1%) Frame = +2 Query: 11 YVVLH*HSQFYIEVSKFITHEFKYSSKMFKFSLFHLLFLATAVQVVLSLREGDCEVCVKT 190 +VVL + ++I F+ K+ +++L L A+ + +L DC T Sbjct: 304 FVVLQARPAHLVSNIQYI-QRFRSPEKLTGEVMYYLSTLMGAMSFIETL---DCSSLTIT 359 Query: 191 VEKFAATLSDDVKK-DPKKIEAGSKSSVKV 277 E+F A + +KK + +K+ S+S V Sbjct: 360 EEEFNAQIEKSIKKMEERKLSEKSESKTAV 389 >SPCC24B10.11c |||THO complex subunit 7 |Schizosaccharomyces pombe|chr 3|||Manual Length = 202 Score = 25.4 bits (53), Expect = 8.2 Identities = 11/35 (31%), Positives = 20/35 (57%), Gaps = 2/35 (5%) Frame = -2 Query: 230 SSHHLIAWRQTSLQFSHTLR--NHLLEVIGPPAQP 132 +++++ AW F TL+ NHL ++ PP+ P Sbjct: 142 NANYVKAWNLRKDIFDETLKQMNHLQSILHPPSNP 176 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,776,465 Number of Sequences: 5004 Number of extensions: 54896 Number of successful extensions: 152 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 149 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 152 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 335201398 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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